Echinacoside Ameliorates Cyclophosphamide-Induced Bladder Damage in Mice

Yunpeng Shao,Yu Liu,Baixin Shen,Qiao Zhou,Zhongqing Wei 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.7

Interstitial cystitis (IC) is featured by apoptosis and chronic inflammation in bladder tissue. We aimed to evaluate the effect of echinacoside (ECH), which is known to modulate inflammation and apoptosis on IC using relevant models. We established a mouse model of cystitis using cyclophosphamide (CYP) and treated human urothelium cells (SV-HUC-1) with lipopolysaccharide (LPS) + ATP as in vitro model. The bladder function was tested by urodynamics. Apoptosis of bladder cells was assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay. Expressions of apoptosis-associated and inflammation-related proteins were assessed using western blotting. Treatment with ECH significantly improved bladder function, reduced inflammatory damage, and decreased apoptosis in the models. Furthermore, ECH decreased the phosphorylation levels of IκB and NF-κB(p65), and upregulated the expression of peroxisome proliferator-activated receptor gamma (PPARγ), which are related to apoptosis and inflammation in CYP-induced mouse cystitis. Moreover, ECH did not reduce apoptosis of urothelial cells after treatment with PPARγ antagonist GW9662. Our findings suggest that ECH might have protective effect against IC in bladder and be mediated through modulation of the PPARγ/NF-κB pathway.

Paleostress reconstructions and stress regimes in the Nanchuan region of Sichuan Basin, South China: implications for hydrocarbon exploration

Wei Ju,Jilin Wang,Huihuang Fang,Yunpeng Gong,Shanjin Zhang 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.4

The Longmaxi shales are the major hydrocarbon source rocks for Sichuan Basin and adjacent regions in China. Tectonic stress field is important in controling the migration and accumulation of shale gas. In the present study, natural fractures in the Nanchuan region of Sichuan Basin were extensively studied and analyzed with the Rotational Optimization Method (ROM) to reconstruct paleostress fields and their transformation processes. The dominant strikes of natural fractures were in the NW-SE, NESW, NWW-SEE and NEE-SWW directions with high-angle oblique or vertical (70~90°) dip angles. Paleostress reconstructions indicated that the Late Yanshanian and Late Himalayan stress fields controlled the tectonics in the Nanchuan region. The orientation of the maximum principal stress (σ1) during the Late Yanshanian and Late Himalayan period was ~NW-SE and ~NE-SW trending, respectively. In addition, the majority of stress regimes during the two tectonic stages were strike-slip regimes (SS). Remote effects of the westward subduction of the paleo-Pacific plate activated the Xuefengshan tectonic zone and generated the Late Yanshanian paleostress field. Later, the change in the stress field was due to the variation of tectonic activity intensity in the Xuefengshan and Dabashan tectonic zones. The paleotectonic stress fields and their transformation processes comprehensively influenced the migration and accumulation of hydrocarbons in the Nanchuan region.

Declined Preoperative Aspartate Aminotransferase to Neutrophil Ratio Index Predicts Poor Prognosis in Patients with Intrahepatic Cholangiocarcinoma after Hepatectomy

Lingyun Liu,Wei Wang,Yi Zhang,Jianting Long,Zhaohui Zhang,Qiao Li,Bin Chen,Shaoqiang Li,Yunpeng Hua,Shunli Shen,Baogang Peng 대한암학회 2018 Cancer Research and Treatment Vol.50 No.2

Purpose Various inflammation-based prognostic biomarkers such as the platelet to lymphocyte ratio and neutrophil to lymphocyte ratio, are related to poor survival in patients with intrahepatic cholangiocarcinoma (ICC). This study aims to investigate the prognostic value of the aspartate aminotransferase to neutrophil ratio index (ANRI) in ICC after hepatic resection. Materials and Methods Data of 184 patients with ICC after hepatectomy were retrospectively reviewed. The cut-off value of ANRI was determined by a receiver operating characteristic curve. Preoperative ANRI and clinicopathological variables were analyzed. The predictive value of preoperative ANRI for prognosis of ICC was identified by univariate and multivariate analyses. Results The optimal cut-off value of ANRI was 6.7. ANRI was associated with tumor size, tumor recurrence, white blood cell, neutrophil count, aspartate aminotransferase, and alanine transaminase. Univariate analysis showed that ANRI, sex, tumor number, tumor size, tumor differentiation, lymph node metastasis, resection margin, clinical TNM stage, neutrophil count, and carcinoembryonic antigen were markedly correlated with overall survival (OS) and disease-free survival (DFS) in patients with ICC. Multivariable analyses revealed that ANRI, a tumor size > 6 cm, poor tumor differentiation, and an R1 resection margin were independent prognostic factors for both OS and DFS. Additionally, preoperative ANRI also had a significant value to predict prognosis in various subgroups of ICC, including serum hepatitis B surface antigennegative and preoperative elevated carbohydrate antigen 19-9 patients. Conclusion Preoperative declined ANRI is a noninvasive, simple, and effective predictor of poor prognosis in patients with ICC after hepatectomy.

Epsilon-Fe₂O₃ is a novel intermediate for magnetite biosynthesis in magnetotactic bacteria

Tong Wen,Yunpeng Zhang,Yuanyuan Geng,Junquan Liu,Abdul Basit,Jiesheng Tian,Ying Li,Ji-Lun Li,Jing Ju,Wei Jiang 한국생체재료학회 2019 생체재료학회지 Vol.23 No.3

Background: Natural biological magnetite nanoparticles are widely distributed from microorganisms to humans. It is found to be very important in organisms, especially in navigation. Moreover, purified magnetite nanoparticles also have potential applications in bioengineering and biomedicine. Magnetotactic bacteria (MTB) is considered one of the most abundant species around the world which can form intracellular membrane enveloped magnetic nanoparticles, referred to as magnetosomes. To our knowledge, the biomineralization of magnetosome in MTB involves a serious of genes located on a large unstable genomic region named magnetosome island, which specially exists in MTB. The magnetite core of magnetosome formed via a Fe (III) ion intermediates, for instance, α-Fe2O3 and ferrihydrite. Though the biosynthesis of magnetosome represents a general biomineralization mechanism of biogenic magnetite, knowledge of magnetosome biosynthesis and biomineralization remains very limited. Method: Cells used in this study were cultured in a 7.5-L bioreactor, samples for intermediate capture were taken each certain time interval after the generation of magnetosome biosynthesis condition. High-resolution transmission electron microscopy were used to analyze the detailed structure of magnetosomes. The parameters of the crystal structures were obtained by Fast Fourier Transform analyses. Results: In this study, we identified a novel intermediate phase, ε-Fe2O3, during the magnetite maturation process in MTB via kinetic analysis. Unlike α-Fe2O3, which has been reported as a precursor during magnetosome biosynthesis in MTB before, ε-Fe2O3, due to its thermal instability, is a rare phase with scarce natural abundance. This finding confirmed that ε-Fe2O3 is an important novel intermediate during the biomineralization of magnetosome in MTB, and shed new light on the magnetosome biosynthesis pathway.

An Experimental and Numerical Study of Corona in a Cage with Sandy and Dusty Flow in High Altitude Area

Yukun Lv,Zekun Ge,Yunpeng Liu,Lei Zhu,Shaoke Wei 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.4

In order to study the effect of the high-altitude and dusty weather in northwest of China on the corona characteristics of transmission lines, a corona caged based experimental system with sandy and dusty flow condition is numerically investigated and designed. This system overcomes the difficulties caused by harsh environment and offers easy usage for off-site tests. The design parameters are mainly determined by the characteristics of strong sandstorm in northwest region and test requirements. By the comparison of numerical simulation of the particle diffusion in four programs with rectangular or circular air-duct, a practical technology, which introduces swirl to control the particle diffusion length, is obtained. Accordingly, the structure of round air-duct with swirl elbow in inlet and outlet of high level segment is selected as final program. Systems of control and measurement are designed at the same time. Field tuning results show that the test system could ensure the range of sandy and dusty coverage. The wind speed, sandy and dusty concentration could be controlled and meet the requirements of accuracy. The experimental system has many features, such as simple structure, easy to be assembled, disassembled, transported and operated, small space occupied.

Residual current fault type recognition based on S3VM and KNN cooperative training

Zhang, Xiangke,Wang, Yajing,Dou, Zhenhai,Wang, Wei,Bai, Yunpeng The Korean Institute of Power Electronics 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.11

It is difficult to detect the residual current of specific fault types in low-voltage distribution networks, which results in few labeled residual current samples. Thus, it is difficult to recognize the fault types of residual current. To solve this problem, a cooperative training classification model based on an improved squirrel search algorithm (ISSA) for a semi-supervised support vector machine (S3VM) and the k-nearest neighbor (KNN) is proposed (ISSA-S3VM-KNN). First, the residual current is decomposed into k intrinsic mode functions (IMFs) by variational mode decomposition (VMD), and the characteristic parameters of the IMFs are extracted to obtain a characteristic dataset for establishing a classification model. Second, to solve the problem where it is difficult to the select parameters (such as the penalty factors, slack variables and kernel function) of a S3VM, an ISSA parameter optimization method is proposed to self-adaptively select the optimal combination of parameters for the S3VM. Finally, the KNN is used to verify the classification results of an ISSA-S3VM through cooperative training, which further improves the classification accuracy of the S3VM for unlabeled residual current samples. Classification results of measured and simulation data show that the classification accuracy of the ISSA-S3VM-KNN is higher than that of the SVM-BPNN, WE-AE-BPNN, and PSO-SVM. The ISSA-S3VM-KNN provides a certain theoretical basis for achieving fast and accurate residual current fault type recognition.

An Experimental and Numerical Study of Corona in a Cage with Sandy and Dusty Flow in High Altitude Area

Lv, Yukun,Ge, Zekun,Liu, Yunpeng,Zhu, Lei,Wei, Shaoke The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.4

In order to study the effect of the high-altitude and dusty weather in northwest of China on the corona characteristics of transmission lines, a corona caged based experimental system with sandy and dusty flow condition is numerically investigated and designed. This system overcomes the difficulties caused by harsh environment and offers easy usage for off-site tests. The design parameters are mainly determined by the characteristics of strong sandstorm in northwest region and test requirements. By the comparison of numerical simulation of the particle diffusion in four programs with rectangular or circular air-duct, a practical technology, which introduces swirl to control the particle diffusion length, is obtained. Accordingly, the structure of round air-duct with swirl elbow in inlet and outlet of high level segment is selected as final program. Systems of control and measurement are designed at the same time. Field tuning results show that the test system could ensure the range of sandy and dusty coverage. The wind speed, sandy and dusty concentration could be controlled and meet the requirements of accuracy. The experimental system has many features, such as simple structure, easy to be assembled, disassembled, transported and operated, small space occupied.

Synthesis of mesoporous PrxZr1-xO2-δ solid solution with high thermal stability for catalytic soot oxidation

Hongcheng Li,Kongzhai Li,Xing Zhu,Yunpeng Du,Yonggang Wei,Kang Zhai,Hua Wang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-

The fabrication of mesoporous PrxZr1xO2d solid solutions with different Pr/Zr molar ratios is reported in the present work. Based on a systematic investigation, the anion of Zr precursor and template species are found to play the most important roles for the formation of mesoporous structure. The triblock copolymer Pluronic F127 template helps to generate porosity in the matrix by self-assembly into micelle, while the additional Cl1 from Zr precursor can lead to the enhancement of the interaction between non-ionic micelles. The physicochemical properties of the obtained samples were studied by means of N2 sorption analysis, TEM, XRD, H2-TPR, O2-TPD and XPS technique. It is found that, compared with the nonmesoporous counterparts, the mesoporous PrxZr1xO2d solid solutions possess much higher specific surface area (139 m2 g1) and enhanced redox ability. Also, the ability to release oxygen is improved after aging at high temperature. For the catalytic soot oxidation, the fresh mesoporous Pr0.1Zr0.9O2d catalyst (calcinated at 500 C) shows high activity in a loose contact condition (T50 = 399 C). It should be highlighted that the T50 values over the three mesoporous PrxZr1xO2d samples decrease by 8–24 C after aging at 800 C for 3 h. This suggests that the mesoporous PrxZr1xO2d solid solution is an ideal candidate for catalytic application at high temperatures.

Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats

Mengyun Li,Wu-Qing OuYang,Xiaoli Wu,Yin Zheng,Yunpeng Wei,Lei An 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.3

Kinetin (Kn) is a cytokinin growth factor that exertsseveral anti-aging and antioxidant effects on cells andorgans. To investigate the mechanism underlying apoptoticevents in aging cells induced by D-galactose (D-gal), weexamined the effect of Kn delivered via nuchalsubcutaneous injection on D-gal-induced aging andapoptosis in rats. Our results showed that interleukin (IL)-2levels and mitochondrial membrane potential (Δ Ψ m) weredecreased by Kn in aging rats while IL-6 production andapoptosis increased. In addition, the expression ofanti-apoptotic Bcl-2 was low while that of Bax was high inthe aging group. After treated with Kn, compared withaging group, there showed obvious difference in Kn groupwith elevated IL-2, proliferation index, Bcl-2, Δ Ψ m anddecreased IL-6 and Bax in splenic lymphocyte. Based onthese results, we concluded that Kn can effectively protectthe rat spleen from aging, apoptosis, and atrophy.

The role of CTNNB1 and LEF1 in feather follicles development of Anser cygnoides and Anser anser

Yue Sun,Yuxuan Zhou,Petunia Msuthwana,Jing Liu,Chang Liu,Cornelius Tlotliso Sello,Yupu Song,Ziqiang Feng,Shengyi Li,Wei Yang,Yunpeng Xu,Xiaomin Yan,Chuanghang Li,Yujian Sui,Jingtao Hu,Yongfeng Sun 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background Wingless-types/beta-catenin (Wnt/β-catenin) signaling pathway is one of the most extensively studied transcriptional cascades involved in various types of organogenesis including embryonic and postnatal development. Downy feather quantity is primarily affected by follicular development and gene regulations. Objective This research was aimed to investigate the role of catenin beta-1(CTNNB1) and lymphoid enhancerbinding factor-1 (LEF1) on feather follicles development at different developmental stages. Methods Fluorescence quantitative PCR, Western-blot and immunohistochemical methods were used in Anser cygnoides and Anser anser embryos (E12, E13 E18, and E28) and after birth gosling stages (G18, G48, G88) for gene expression analysis. Results CTNNB1 and LEF1 genes were expressed in Anser cygnoides and Anser anser at different embryonic and after-birth gosling developmental stages and the expression levels were significantly different in different stages (p < 0.05). The mRNA expression of CTNNB1 and LEF1 genes reached the highest level at D88 in Anser cygnoides, while the highest expression levels were at D18 and D88 in Anser anser, and the expression levels of CTNNB1 genes at D88 in all embryonic stages were significantly lower than after-birth stages. CTNNB1 and LEF1 protein expression were the highest at E12 and E28 for Anser cygnoides feather follicles development. While at a similar stage for Anser anser, the expression of CTNNB1 and LEF1 protein was the highest at D48 and D18. Protein expression at embryonic stages was in the epidermis (E) and the hair basal plate (P), the expression site for after-birth stages was in the dermal papilla (DP). Conclusion Our study illustrated that CTNNB1 and LEF1 has an impact on Anser cygnoides and Anser anser feather follicles growth and development.