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Characteristics of isolated lactic acid bacteria and their effects on the silage quality
Siran Wang,Xianjun Yuan,Zhihao Dong,Junfeng Li,Gang Guo,Yunfeng Bai,Junyu Zhang,Tao Shao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6
Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures (10°C, 15°C, and 25°C). Results: All isolated strains (LCG3, LTG7, I5, and LI3) could grow at 5°C to 20°C, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/ total nitrogen (AN/TN) compared with uninoculated silages at various temperatures (10°C, 15°C, and 25°C). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at 10°C and 15°C, indicated by similar pH, LA content and AN/TN. Conclusion: All inoculants could improve the silage fermentation quality at various temperatures (10°C, 15°C, and 25°C). At the temperature of 10°C and 15°C, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.
Cellular U-Shaped Steel Combination Column Seismic Performance Analysis
Yunfeng Li,Cheng Zhao,Qianqian Lu 한국강구조학회 2021 International Journal of Steel Structures Vol.21 No.5
In this paper, a new form of structural design of the combination column—Cellular U-shaped steel combination column, as vertical force components applied to steel structure buildings. By changing the diameter size of honeycomb holes, the thickness of U-shaped steel and embellished plates, and the steel strength, the seismic properties of this new combination steel column are studied by using ABAQUS fi nite element software. Results the setting of honeycomb hole is reduced by part, the delay of the test piece and its energy consumption capacity are improved to varying degrees, the stiff ness degradation rate is improved, and the setting of honeycomb steel plays a certain role in the seismic performance of the steel column. The experiment is used as a reference, and the destruction form and carrying capacity are compared, so that the numerical model has some credibility. Results the strength and stiff ness of the cell U-shaped steel grid composition column are analyzed to meet the standards, the seismic performance is good, and can be applied to engineering practice.
Yan Li,Naisen Yu,Haiou Li,Dedi Liu,Yunfeng Wu 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.6
In this paper, a novel individual multiple-junction network structure is fabricated using low temperature aqueous method. In comparison with the same growth method without NaF adding, the Fluorine doped ZnO photodetector presents multiplejunctionnetwork structure and ultraviolet (UV)/Blue dual band photoresponse performances. The results indicate the method can simultaneously promote self-assembled growth and improve the photoresponse properties. Moreover, it will provide a simple and low cost way to assemble multiple nanowires for photoresponse applications.
Li, Wenting,Zhang, Mengmeng,Wang, Kejun,Lu, Yunfeng,Tang, Hui,Wu, Keliang Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: The objective of a conservation program is to maintain maximum genetic diversity and preserve the viability of a breed. However, the efficiency of a program is influenced by the ability to accurately measure and predict genetic diversity. Methods: To examine this question, we conducted a simulation in which common measures (i.e. heterozygosity) and novel measures (identity-by-descent probabilities and parental genomic components) were used to estimate genetic diversity within a conserved population using double-labeled single nucleotide polymorphism markers. Results: The results showed that the accuracy and sensitivity of identity-by-state probabilities and heterozygosity were close to identity by descent (IBD) probabilities, which reflect the true genetic diversity. Expected heterozygosity most closely aligned with IBD. All common measures suggested that practices used in the current Chinese pig conservation program result in a ~5% loss in genetic diversity every 10 generations. Parental genomic components were also analyzed to monitor real-time changes in genomic components for each male and female ancestor. The analysis showed that ~7.5% of male families and ~30% of female families were lost every 5 generations. After 50 generations of simulated conservation, 4 male families lost ~50% of their initial genomic components, and the genomic components for 24.8% of the female families were lost entirely. Conclusion: In summary, compared with the true genetic diversity value obtained using double-labeled markers, expected heterozygosity appears to be the optimal indicator. Parental genomic components analysis provides a more detailed picture of genetic diversity and can be used to guide conservation management practices.
Feasibility study of wide-band low-profile ultrasonic sensor with flexible piezoelectric paint
Li, Xin,Zhang, Yunfeng Techno-Press 2008 Smart Structures and Systems, An International Jou Vol.4 No.5
This paper presents a feasibility study of flexible piezoelectric paint for use in wide-band low-profile surface-mount or embeddable ultrasonic sensor for in situ structural health monitoring. Piezoelectric paint is a piezoelectric composite with 0-3 connectivity. Because of its ease of application, piezoelectric paint can be readily fabricated into sensing element with complex pattern. This study examines the characteristics of piezoelectric paint in acoustic emission signal and ultrasonic guided wave sensing. A series of ultrasonic tests including pitch catch and pencil break tests were performed to validate the ultrasonic wave sensing capability of piezoelectric paint. The results of finite element simulation of ultrasonic wave propagation, and acoustic emission generated by a pencil lead break on an aluminum plate are also presented in this paper along with corresponding experimental data. Based on the preliminary experimental results, the piezoelectric paint appears to offer a promising sensing material for use in real-time monitoring of crack initiation and propagation in both metallic and composite structures.
LI HOU,Yunfeng Niu,Yan Wang,YANG JIANG,Rongna Chen,Tianran Ma,FAMING GAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.6
Here, we present a simple and novel method based on using insulin amyloid fibrils (INSAFs) as sacrificial templates for the construction of Pt–Pd nanoparticle chains (Pt–PdNPCs) under mild conditions. By incubating INSAFs in metal salt solution and then reduction, Pt–Pd nanoparticles with an uniform particle size of around 2.5 nm nucleated and grew along the axial direction of INSAFs, and thus formed a long chain structure with length up to several micrometers. The as-prepared Pt–PdNPCs exhibit an improved catalytic activity for lowtemperature CO and methanol oxidation and possess greater CO tolerance compared with the commercial Pt/C catalyst, which makes them promising for a variety of possible catalytic applications.
Shen Yunfeng,Li Xiucun,Zhang Chao,Zhong Hai,Dou Weiqiang 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.8
Objective: To investigate the clinical application of differential subsampling with Cartesian ordering (DISCO) contrastenhanced (CE) magnetic resonance angiography for anterolateral thigh (ALT) flap transplantation, using operative findings as a reference. Materials and Methods: Thirty patients (21 males and nine females; mean age ± standard deviation, 45.5 ± 15.6 years) who were scheduled to undergo reconstruction with ALT flaps between June 2020 and June 2021 were included in the prospective study. Before ALT flap transplantation, patients were scanned using CE-DISCO imaging. All acquired DISCO images of the 60 lower limbs (both sides from each patient) were analyzed using maximum intensity projection and volume rendering methods. Two experienced radiologists were employed to examine the patterns of the lateral circumflex femoral artery (LCFA), its branches, and perforators and their skin termini, which were compared with the operative findings. Results: Using CE-DISCO, the patterns of the LCFA and its branches were clearly identified in all patients. Four different origins of the LCFA were found among the 60 blood vessels: type I (44/60, 73.3%), type II (6/60, 10.0%), type III (8/60, 13.3%), and type IV (2/60, 3.3%). Owing to a lack of perforators entering the skin, two patients did not undergo ALT flap transplantation. For the remaining 28 patients, the ALT flaps in 26 patients were successfully operated without flap reselection during the operation, while the remaining two patients underwent other surgical procedures due to the thin diameter of the perforator or injury of the perforator during the operation. The success rate of flap transplantation was 92.8% (26/28). All transplanted flaps exhibited good blood supply and achieved primary healing without infection or delayed healing. Conclusion: CE-DISCO imaging can be an effective method for preoperative perforator imaging before ALT flap transplantation.