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동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여
박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1
This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.
( Bo Xu ),( Fu Ya Yang ),( Cai Yun Xiong ),( Jun Jun Li ),( Xiang Hua Tang ),( Jun Pei Zhou ),( Zhen Rong Xie ),( Jun Mei Ding ),( Yun Juan Yang ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-amylase. AmyPL was expressed in Escherichia coli BL21 (DE3) and the purified AmyPL was enzymatically characterized. This study is the first to report the molecular and biochemical characterization of a novel α-amylase from a gastrointestinal metagenomic library.
양보경(Bo-Kyung Yang),양윤정(Yun-Jung Yang) 한국문화역사지리학회 2006 문화 역사 지리 Vol.18 No.1
본 논문은 조선후기에 제작된 木版本 조선전도인 《海左全圖》의 내용과 판본 분석을 통해 지도의 유형과 특정을 파악하고, 조선후기 목판본 지도 제작의 보급과 그 중요성을 밝히는데 목적이 있다. 목판본 해좌전도 의 전체 윤곽은 농포자 鄭尙驥의 《東國地圖》 계열의 조선전도와 유사하며, 여러 종의 지도를 종합하여 1857년에서 1859년 사이에 제작된 것으로 추정된다. 이 지도는 유사한 크기의 다른 지도에 비해 많은 정보를 포함하고 있으며, 이를 가능하게 한 가장 큰 특징은 지도 여백에 판각된 註記이다. 《海左全圖》는 세로 1m 정도의 크기로서 휴대용으로 적절하며, 많은 정보가 수록되어 있어 地誌的 요소가 풍부해 지역에 관한 풍부한 자료를 제공하는 특징을 지녔다. 또한 다량 제작이 가능한 木版本 地圖로서, 비슷한 판본이 여러 차례 제작된 것은 조선시대에 대중적인 지도로 널리 이용되었음을 보여 준다. 총 17장의 《海左全圖》를 대상으로 판본을 검토 분석한 결과 지도의 크기나 형태ㆍ내용은 유사하지만 전혀 다른 3종류의 판본이 있음을 발견했다. 이는 목판본으로 제작된 《海左全圖》가 지도의 대중성과 지도의 내용성을 동시에 만족시켰던 지도임을 보여 준다. 이를 통해 《海左全圖》를 조선 후기의 지도 특히 목판본 지도의 이용과 제작의 확대를 보여주는 대표적인 지도로 자리매김할 수 있다. This paper aims to study of the types of the general map Haejwajeondo printed from wood blocks and the importance of woodcut print maps in the late Joseon Period. The one meter length of wooden plates of Haejwajeondo are thought to be made between 1857 and 1859. The overall style is similar to Jeong Sanggi(1678~1752)-style maps. Haejwajeondo was supposed to be made by assembling different maps. The explanatory notes written in the spaces of the map is one of the most outstanding features of the map, and they make the Haejwajeondo includes more information than other maps that are similar in size. A one-meter Haejwajeondo was small enough to carry around and it contained not only a lot of geographic information. Comparing seventeen woodblock print maps of Haejwajeondo led to a finding that they are divided into completely different three types. It shows the map seemed to be used widely during the nineteenth century. It also demonstrates the importance and increase of the woodblock print maps in late Joseon Period.
The effects of benzene and toluene on leukotactin-1-induced migration of EoL-1 cells
Song, Bo-Bae,Yang, Eun-Ju,Kim, Bo-Mi,Lee, Ji-Sook,Yun, Chi-Young,Im, Yang-Bin,Kim, In-Sik 대한독성유전단백체학회 2011 Molecular & cellular toxicology Vol.7 No.2
Benzene and toluene are volatile organic compounds (VOCs) that are produced from various building materials and may lead to sick building syndrome. In addition, these materials exert cytotoxic and carcinogenic effects in immune cells. To understand the effects of VOCs on immunological regulation, we investigated the effects of VOCs on cell migration in response to CC chemokines such as leukotactin-1 (Lkn -1), monocyte chemoattractant protein-1 (MCP-1), eotaxin, $MIP-1{\alpha}$ and RANTES in human eosinophilic leukemia EoL-1 cells. VOCs exerted no cytotoxicity against EoL-1 cells at concentrations ranging from $0.1\;{\mu}M$ to $50\;{\mu}M$. A chemotaxis assay was conducted to evaluate the cell movement. The assay revealed that benzene and toluene differentially increased the migration of EoL-1 cells in response to Lkn-1, but not MCP-1, eotaxin, $MIP-1{\alpha}$ or RANTES. The expression of CCR1 and CCR3 binding to Lkn-1 were not altered by benzene and toluene. Additionally, the increased cell migration due to benzene and toluene was inhibited by PD98059, SB202190 and SP600125. Benzene and toluene also induced the phosphorylation of ERK, p38 mitogen-activated protein kinase (p38 MAPK) and JNK in a time-dependent manner. Overall, benzene and toluene influenced Lkn-1-induced migration of human eosinophilic cells via activation of MAPKs. These results suggest that benzene and toluene play a role as risk factors in the regulation of immune response. Furthermore, these findings provide a role for VOCs in the immunological processes involved in indoor air pollution-induced diseases.
Reliability Evaluation of Power System Operations Considering Time-Varying Features of Components
Bo Hu,Ying Zheng,Hejun Yang,Yun Xia 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.4
The reliability of power system components can be affected by a numbers of factors such as the health level of components, external environment and operation environment of power systems. These factors also affect the electrical parameters of power system components for example the thermal capacity of a transmission element. The relationship of component reliability and power system is, therefore, a complex nonlinear function related to the above-mentioned factors. Traditional approaches for reliability assessment of power systems do not take the influence of these factors into account. The assessment results could not, therefore, reflect the short-term trend of the system reliability performance considering the influence of the key factors and provide the system dispatchers with enough information to make decent operational decisions. This paper discusses some of these important operational issues from the perspective of power system reliability. The discussions include operational reliability of power systems, reliability influence models for main performance parameters of components, time-varying reliability models of components, and a reliability assessment algorithm for power system operations considering the time-varying characteristic of various parameters. The significance of these discussions and applications of the proposed techniques are illustrated by case study results using the IEEE-RTS.
P237 : A study of histamine content in Korean traditional foods
( Yun Sun Byun ),( Yong Se Cho ),( Yoon Seok Yang ),( Jin Hye Kim ),( Bo Young Chung ),( Hye One Kim ),( Chun Wook Park ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Ingestion of foods that contain much histamine can provoke not only signs and symptoms of histamine poisoning such as nausea, vomiting, and diarrhea, but also allergic reactions such as rash and pruritus. Objectives: The purpose of this study is to present a list of Korean traditional foods containing great amounts of histamine. Methods: The histamine level of foods was measured by using enzyme-linked immunosorbent assay (ELISA). Results: Fermented pastes, such as soybean paste (1164 ng/g) and red pepper paste (1448 ng/g), showed high histamine levels. There wasn’t no difference in histamine level depending on cooking methods, such as boiling. Fermented cabbage (378 ng/g) and radish (500 ng/g), such as kimchi, showed 30 to 50 times higher histamine levels than the ones of fresh cabbage (11 ng/g) and fresh radish (10 ng/g). Salty fermented anchovies showed high histamine levels (1157 ng/g), but salty fermented shrimps (27 ng/g) showed low histamine levels, as did fresh shrimps (24 ng/g). Conclusion: We suggest that this study is used as a guideline for educational dietary interventions for patients with chronic urticaria or histamine intolerance.