Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

Recent advances of bioactive proteins/polypeptides in the treatment of breast cancer

Qi-Zhang Li,Ze-Rong Zhou,Cui-Yu Hu,Xian-Bin Li,Yu-Zhou Chang,Yan Liu,Yu-Liang Wang,Xuan-Wei Zhou 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.3

Proteins do not only serve as nutrients to fulfill the demand for food, but also are used as a source of bioactive proteins/polypeptides for regulating physical functions and promoting physical health. Female breast cancer has the highest incidence in the world and is a serious threat to women’s health. Bioactive proteins/polypeptides exert strong anti-tumor effects and exhibit inhibition of multiple breast cancer cells. This review discussed the suppressing effects of bioactive proteins/polypeptides on breast cancer in vitro and in vivo, and their mechanisms of migration and invasion inhibition, apoptosis induction, and cell cycle arrest. This may contribute to providing a basis for the development of bioactive proteins/polypeptides for the treatment of breast cancer.

Expression analysis of HSP70 gene in response to environmental stress in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Zhou Lv,Meng Jian-Yu,Ruan Hong-Yun,Zhang Chang-Yu 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.3

This study aimed to investigate the role of heat shock proteins in the response of Spodoptera frugiperda to environmental stress. Four Hsp70 genes of S. frugiperda were cloned using reverse transcription–polymerase chain reaction (RT-PCR) and their sequence characteristics were characterized using bioinformatics analysis. RTqPCR was used to detect Hsp70 at different developmental stages, in different tissues, and under varying environmental stresses (45 ◦ C, 36 ◦ C, 4 ◦ C, ultraviolet-A, and emamectin benzoate) to quantify the relative expression of this gene. Four Hsp70 genes were obtained by cloning, namely SfHsp70-1, SfHsp70-2, SfHsp70-3, and SfHsp70-like, which encode 653, 686, 638, and 627 amino acids, respectively, and all contain the three Hsp70 family signature sequences. Neighbor-joining (NJ) method was used to construct phylogenetic tree, and four Hsp70 of the S. frugiperda species were clustered with Lepidoptera. The results of real-time PCR showed that the SfHsp70s gene was specifically expressed in different tissues at different developmental stages and its expression increased considerably under nearly all environmental stresses in adults. This study shows that the SfHsp70 gene contributes to a large extent to the resistance of S. frugiperda to environmental stress.

Enhancing densification and electrical properties of KNN-based lead-free ceramics via two-step sintering

Zhou Yu,Fang Bijun,Zhang Shuai,Lu Xiaolong,Ding Jianning 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.5

0.95(Li 0.02 Na 0.50 K 0.48 )(Nb 0.95 Sb 0.05 )O 3 -0.05AgTaO 3 (0.95LNKNS-0.05AT) lead-free piezoelectric ceramics were prepared by conventional ceramic processing via the two-step sintering process (abbreviated as TSS). The influences of sintering condi- tions in the first stage of the TSS process on densification, dielectric and piezoelectric properties of the 0.95LNKNS-0.05AT ceramics prepared by the TSS were deeply studied. By introducing TSS for preparing the 0.95LNKNS-0.05AT ceramics, the volatilization of alkali metal elements can be decreased and all the 0.95LNKNS-0.05AT ceramics show high densifica- tion. By optimizing the T 1 sintering temperature and t 1 holding time in the first stage of the TSS, the relative permittivity of the 0.95LNKNS-0.05AT ceramics sintered at 1140 °C for 0.5 h and 1050 °C for 8 h is increased to 5957 at 1 kHz, while the relative permittivity of the 0.95LNKNS-0.05AT ceramics prepared by the conventional sintering method (abbreviated as CS) is only 5205. The d 33 , g 33 , and K p values are increased from 134 pC/N, 14.6 × 10 –3  Vm/N, and 17.4% to 224 pC/N, 25.5 × 10 –3  Vm/N, and 31.9%, respectively. The 0.95LNKNS-0.05AT ceramics exhibit thermal activation relaxation behav- ior, and the high-temperature Cole–Cole impedance performance can be fitted well by the equivalent circuit (RQC) and equivalent circuit (RC) connected in series. The space charge effect conforms to the Arrhenius relationship, which presents a thermally activated hopping type process and relates to the oxygen vacancies generated by the evaporation of alkali metals during the TSS process.

Improved Method for Calculating Armature-Reaction Field of Surface-Mounted Permanent Magnet Machines Accounting for Opening Slots

Zhou, Yu,Li, Huaishu,Wang, Qingyu,Xue, Zhiqiang,Cao, Qing,Zhou, Shi The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.4

This paper presented an improved analytical method for calculating armature-reaction field in the surface-mounted permanent magnet machines accounting for opening slots. The analytical model is divided into two types of subdomains. The current of the armature is centralized in the center of the slots. The field solution of each subdomain is obtained by applying the interface and boundary conditions of the model. Two 30-pole/36-slot prototype machines with different slot-opening width are used for validation. The FE (finite element) results confirm the validity of the analytical results with the proposed model. The investigation shows that the wider the slot-opening width is, the smaller the peak value of radial and circumferential components of flux density, and the analytical armature-reaction field produced by centralized current in the slots is similar with the armature-reaction field produced by distributed current in the slots in the FE.

중국 영상 문화에 끼친 한류의 영향 : 영화와 드라마의 경우를 중심으로

Zhou Yu bo(주오유보) 한국언론학회 2005 한국언론학회 연구보고서 및 기타간행물 Vol.- No.-

As a fresh impact wave of the popular culture, the success and affection to "Korean wave" can not be neglected whatever the neighbour countries like it or not. It is more important that this wave has been beyond the scope of the public culture and developed into a more realistic economy benefit, which contributes to the Korean economy, meanwhile attributes a new world acknowledgement Korea and it"s people and even changes the impression of Korean substantially. "Korean wave" was first occurred in China, from this point; China could be an original basement of this wave. If the passion singing in the Seoul Olympic games in 1988 gave the Chinese people a new image of Korea, a series of Korea TV programs make the Chinese people know that the Korea is a nation full of confidence, moral courage, value emotion and dare to chase what they want. The hot show of Korean TV play brought about culture effects, and gave a lot of new concepts to Chinese audiences and TV program workers. Chinese TV program making industry began to use the "Korean wave", borrow the successful experiences to use it in home-made movies, try to make the program that match the taste of Chinese audiences and fulfill the needs of domestic audiences. They conduct two applicable methods. One is co-operate with Korea TV making company, the other is to follow the lead of the "Korean wave", made domestic "Korea" TV play. Usually it is called youth idol TV play. The writer will analyze the get and lost of the TV play "Beijing, My Love"and "Apartment of Whitecollar". Why does the "Korean wave" have achieved so much success in the Chinese scream culture market? In my opinion, the main reasons are as follows: Firstly, it is partly due to the characteristics of the transform period in Chinese politics, economy, and culture. Secondly, the visual culture of "Korean wave" has its obvious advantages. Thirdly, after reform open, the consciousness of the Chinese young generation variety has played an important role in the spread of the "Korean wave". Fourthly, the "Korean wave" has comparison advantage in contrast with other substitute cultures from Japan, Hong Kong and Taiwan etc. Fifthly, with the promotion and participation from the Korea government, Korea play promote circulation in world culture trade. Sixthly, multinational culture capital takes part in, makes use of the "Korean wav e". and obtains enormous market success. In some aspects, the success of Korean visual culture lies in its dialectic attitude of own culture inheritance and development. Korea is not the simple culture receiver in the past any more, but a culture broadcaster at present, which produce considerable influence on neighbour countries. Then China ought to adapt to this new situation and should neither completely accept Korean wave nor treat it as virus. Culture lives in its mobility and its interaction. and a civilization would be more powerful only when it accepts outside stimulations and challenges now and then. Pluralism and mutual beneficial interaction in culture be the only final direction that "Korean wave" went.

New Permeable Structure for Controlling Debris Flows in the Wenjiagou Gully

Yu-feng Liang,Chuan Liang,Hongwei Zhou,Feng-rong Yang,Miao Huo,Jia-wen Zhou 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.11

Aimed to control debris flows more effectively, we attempt to develop a new structure called a “sloping roof grill dam”, which consists of guidance walls, separation grills and lateral deposit areas. A series of flume tests were performed to verify the function of this new structure, focusing on the trapping efficiency (β ), deceleration efficiency (μ) and coarse-fine sediment separation extents (η) under different conditions of structure and debris flows. Experimental results show that both the trapping and deceleration efficiency increase with a decrease in permeability, whereas the separation extent shown the opposite effect. Multiple regression analysis indicates that β and η are linearly dependent on the permeability, deceleration efficiency and magnitudes of debris flow. In addition, an increase in beam gap can lead to an increase in the impact area downstream for the debris flow after the control of the structure. Finally, a two-level installation of the structure is tested to determine whether there is an enhancement of the debris control, which indicated that although the trapping effect of the two-level structure is considerably greater than that of a single-level structure (2.11 times at maximum), the separation extent of the two-level structure is not distinct and may be even lower.

Steady-State Analaysis of an Interleaved Buck-Boost Converter using Discrete and Coupled Inductors

Zhou Yu(주우 ),Tuan-Vu Le(레동부),Sung-Jun Park(박성준) 대한전기학회 2016 대한전기학회 학술대회 논문집 Vol.2016 No.7

Comprehensive Assessment of Associations between ERCC2 Lys751Gln/Asp312Asn Polymorphisms and Risk of Non-Hodgkin Lymphoma

Zhou, Jue-Yu,He, Li-Wen,Liu, Jie,Yu, Hai-Lang,Wei, Min,Ma, Wen-Li,Shi, Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Excision repair crossing-complementing group 2 (ERCC2), also called xeroderma pigmentosum complementary group D (XPD), plays a crucial role in the nucleotide excision repair (NER) pathway. Previous epidemiological studies have reported associations between ERCC2 polymorphisms and non-Hodgkin lymphoma (NHL) risk, but the results have remained controversial. Materials and Methods: We conducted this meta-analysis based on eligible case-control studies to investigate the role of two ERCC2 polymorphisms (Lys751Gln and Asp312Asn) in determining susceptibility to NHL. Ten case-control studies from several electronic databases were included in our study up to August 14, 2014. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using fixed- or random-effects models to estimate the association strength. Results: The combined results based on all studies did not show any association between Lys751Gln/Asp312Asn polymorphisms and NHL risk for all genetic models. Stratified analyses by histological subtype and ethnicity did not indicate any significant association between Lys751Gln polymorphism and NHL risk. However, a significant reduced risk of NHL was found among population-based studies (Lys/Gln versus Lys/Lys: OR=0.87, 95% CI=0.77-0.99, P=0.037) but not hospital-based studies. As for Asp312Asn polymorphism, there was no evidence for the association between this polymorphism and the risk of NHL in all subgroup analyses. Conclusions: This meta-analysis suggests that there may be no association between Lys751Gln/Asp312Asn polymorphism and the risk of NHL and its two subtypes, whereas ERCC2 Lys751Gln heterozygote genotype may provide protective effects against the risk of NHL in population-based studies. Therefore, large-scale and well-designed studies are needed to clarify the effects of haplotypes, gene-gene, and gene-environment interactions on these polymorphisms and the risk of NHL and its different histological subtypes in an ethnicity specific population.

Improved Method for Calculating Magnetic Field of Surface-Mounted Permanent Magnet Machines Accounting for Slots and Eccentric Magnet Pole

Zhou, Yu,Li, Huaishu,Wang, Wei,Cao, Qing,Zhou, Shi The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

This paper presented an improved analytical method for calculating the open-circuit magnetic field in the surface-mounted permanent magnet machines accounting for slots and eccentric magnet pole. Magnetic field produced by radial and parallel permanent magnet is equivalent to that produced by surface current according to equivalent surface-current method of permanent magnet. The model is divided into two types of subdomains. The field solution of each subdomain is obtained by applying the interface and boundary conditions. The magnet field produced by equivalent surface current is superposed according to superposition principle of vector potential. The investigation shows harmonic contents of radial flux density can be reduced a lot by changing eccentric distance of eccentric magnet poles compared with conventional surface-mounted permanent-magnet machines with concentric magnet poles. The FE(finite element) results confirm the validity of the analytical results with the proposed model.