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Nam, Sang-Ho,Kim, Yu-Na Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.6
A hexavalent chromium (Cr (VI)) is one of the hazardous substances regulated by the RoHS. The determination of Cr (VI) in various polymers and printed circuit board (PCB) has been very important. In this study, the three different analytical methods were investigated for the determination of a hexavalent chromium in Acrylonitrile Butadiene Styrene copolymer (ABS) and PCB. The results by three analytical methods were obtained and compared. An analytical method by UV-Visible spectrometer has been generally used for the determination of Cr (VI) in a sample, but a hexavalent chromium should complex with diphenylcarbazide for the detection in the method. The complexation did make an adverse effect on the quantitative analysis of Cr (VI) in ABS. The analytical method using diphenylcarbazide was also not applicable to printed circuit board (PCB) because PCB contained lots of irons. The irons interfered with the analysis of hexavalent chromium because those also could complex with diphenylcarbazide. In this study, hexavalent chromiums in PCB have been separated by ion chromatography (IC), then directly and selectively detected by inductively coupled plasma atomic emission spectrometry (ICP-AES). The quantity of Cr (VI) in PCB was 0.1 mg/kg.
Large-For-Size Syndrome after Deceased Donor Liver Transplantation: A Case Report
( Yu Mi Kim ),( Sung Ha Lee ),( Jun Suh Lee ),( Jung Hyun Kwon ),( Soon Woo Nam ),( Young Chul Yoon ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Background: Large-for-size (LFS) is a serious problem that can develop during liver transplantation (LT) and is related to morbidities such as insufficient blood supply causing graft dysfunction or impractical closure of the abdominal wall leading to graft compression. LFS is usually discussed in pediatric LT and is often managed by reducing the size of the graft before implantation. In contrast, only a few cases about managing unexpected LFS during adult LT have been reported. Case: The female patient of 44 years of age was admitted to the Incheon St. Mary’s Hospital with drug-induced acute hepatitis and managed conservatively. However, the patient underwent deceased donor liver transplantation (DDLT) due to persistent liver deterioration. Donor was a 51 year old male, weighing 60.0 kg and matched because the patient weighed 58.4 kg. Donor had a history of eating one bottle of Soju daily for 30 years, but the liver biopsy showed no fatty change at 0 %, but the liver size was shown larger than normal. The weight of donor liver was 2040 g, which was much heavier than normal but the liver transplantation was performed uneventfully. However, left lateral sectionectomy was performed because the abdomen was not be closed. On the next day, the liver function was deteriorated and blood pressure was dropped. Immediately, we took computed tomography (CT) scan and were able to observe the narrowing of the inferior vena cava (IVC) by the liver. Venogram showed significant stenosis at IVC suprahepatic portion, so IVC stent placement was performed using 24 x 80 mm Hercules stent graft (MicroPort Scientific Corporation, Shanghai, China). After stenting, blood pressure was stabilized but liver function was deteriorated more and renal replacement therapy was performed because of impaired renal function. However, liver and renal function gradually improved and discharged without any complication. Discussion & Conclusion: LFS can cause serious complication, but can be solved by various treatment modality.
Sung-Won Choi,Nam-Yun Her,Dong-Seob Kim,Sun-Jin Choi,Han-Seung Lee,Hak-Jong Choi,Ju-Hyun Yu 한국식품영양과학회 1997 Preventive Nutrition and Food Science Vol.2 No.2
CMCase produced by recombinant E. coli JM109(pCEH#4) containing CMCase gene from Cellulomonas sp. YE-5 was purified to 24.3 fold and 2.6% yield by ammonium sulfate precipitation, DEAE-cellulose column chromatography and gel filtration on Sephadex G-100. The optimum pH and temperature for CMCase activity were pH 7.0 and 50℃. The enzyme was stable between pH 5.0 and 10.0, and up to 60℃. The molecular weight of the enzyme was estimated to be approximately 40,000 daltons by SDS-PAGE. Analysis of the amino acid composition showed that the enzyme contained many glycines and acidic amino acids. The enzyme was an endo-type CMCase and the final enzyme reaction product from hydrolysis of CM-cellulose by the enzyme was cellobiose. K_M value determined with CM-cellulose was 1.28mM.
Nam, Byung Ho,Kim, Young-Woo,Reim, Daniel,Eom, Bang Wool,Yu, Wan Sik,Park, Young Kyu,Ryu, Keun Won,Lee, Young Joon,Yoon, Hong Man,Lee, Jun Ho,Jeong, Oh,Jeong, Sang Ho,Lee, Sang Eok,Lee, Sang Ho,Yoon, The Korean Gastric Cancer Association 2013 Journal of gastric cancer Vol.13 No.3
Purpose: Laparoscopy-assisted distal gastrectomy for early gastric cancer has gained acceptance and popularity worldwide. However, laparoscopy-assisted distal gastrectomy for advanced gastric cancer is still controversial. Therefore, we propose this prospective randomized controlled multi-center trial in order to evaluate the safety and feasibility of laparoscopy assisted D2-gastrectomy for advanced stage gastric cancer. Materials and Methods: Patients undergoing distal gastrectomy for advanced gastric cancer staged cT2/3/4 cN0/1/2/3a cM0 by endoscopy and computed tomography are eligible for enrollment after giving their informed consent. Patients will be randomized either to laparoscopyassisted distal gastrectomy or open distal gastrectomy. Sample size calculation revealed that 102 patients are to be included per treatment arm. The primary endpoint is the non-compliance rate of D2 dissection; relevant secondary endpoints are three-year disease free survival, surgical and postoperative complications, hospital stay and unanimity rate of D2 dissection evaluated by reviewing the intraoperative video documentation. Discussion: Oncologic safety is the major concern regarding laparoscopy-assisted distal gastrectomy for advanced gastric cancer. Therefore, the non-compliance rate of clearing the N2 area was chosen as the most important parameter for the technical feasibility of the laparoscopic procedure. Furthermore, surgical quality will be carefully reviewed, that is, three independent experts will review the video records and score with a check list. For a long-term result, disease free survival is considered a secondary endpoint for this trial. This study will offer promising evidence of the feasibility and safety of Laparoscopy-assisted distal gastrectomy for advanced gastric cancer. Trial Registration: NCT01088204 (international), NCCCTS-09-448 (Korea).
Behaviour alteration of Topoisomerase IIIβ knockout mice
Yu Rim Kim,Eun Kyoung Kim,Young Sub Byun,Hae Rim Kim,Ju Sung Kang,Ju Young Lee,Min Young Lee,Sang Mi Cho,Ki Hoon Lee,Hoyoung Ghang,Won Kee Yoon,Young Suk Won,Hyoung Chin Kim,Young Hee Lee,Ki Hoan Nam 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8