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Identification of the Gene Products Responsible for F Plasmid Partitioning
KIM, SUNG0UK,NAGAI, KAZUO,TAMURA, GAKUZO,YU, JU-HYUN,BOK, SONG-HAE 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4
DNA subfragments, sopA, sopB and sopC which help to maintain the stability of an on C plasmid, were derived from a mini-F plasmid DNA (EcoRI restriction fragment f5) after digestion with restriction endonuclease, and cloned in the vector plasmid pBR322. The recombinant plasmids obtained were introduced into E. coli KY7231 and E. coli CSR603 strains, and proteins specified by the mini-F fragments were analysed by SDS-PAGE. Two proteins encoded by the F fragments were detected, and their molecular weights were 41,000 and 37,000 daltons. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins had been overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of sopA and sopB proteins were 6.6 and 7.0, respectively.
Seasonal Patterns of Bird Migration at a Stopover Site during the Migratory Period
Yu-Seong Choi,Sung-Hyun Kim,Jong-Seong Son,Seung-Gu Kang,Wee-Haeng Hur,Sang-Hoon Han 한국조류학회II 2013 한국조류학회지 Vol.20 No.1
This study focused on the seasonal pattern of migrant birds on an island that is used as a stopover site when they cross the Korean peninsula. Surveys were conducted on Oeyeon Island (N36°13′38″, E126°04′53″), which is located in the middle area of West Sea of Korea, during the spring (from March to May) and autumn (from September to November) migration seasons of 2011 and 2012. Increased number of individuals and species were observed on the Oeyeon Island during the spring season migration compared to the autumn season migration (mean±SD; species, spring 70.0±25.1 vs. autumn 49.0±9.1; individuals, spring 606.1±259.8 vs. autumn 280.9±77.2). Furthermore, this pattern was also observed for passerine birds (species, spring 47.6±17.4 vs. autumn 30.3±6.9; individuals, spring 518.7±263.6 vs. autumn 219.8±85.8). We suggest that physiological and ecological factors cause differences in the bird migration patterns between spring and autumn on the Korean islands, such as differences in the need for refueling after long-distance flight, selective pressure during the breeding season, and seasonal variation in the migration routes.
Conformational Characteristics of p-tert-Butylcalix[6]arene Mono-Alkyl Ester Derivatives
Yu, Hyo-Yeon,Seok, Da-Hyun,Chang, Suk-Kyu,Ahn, Sang-Doo,Chung, Kee-Choo Korean Magnetic Resonance Society 2010 Journal of the Korean Magnetic Resonance Society Vol.14 No.2
Conformational properties of several mono-alkyl ester derivatives of p-tert-butylcalix[6]arene depending on temperatures have been investigated by solution NMR spectroscopy. It is found out that all derivatives can adopt a cone-like conformation around room temperature, while the conformational characteristics at high temperatures seem to be quite different each other.
Topology optimization using spline finite element method and trimmed surfaces
Sung-Kie Youn,Yu-Deok Seo,Hyun-Jung Kim 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.3
In the present work, a new topology optimization based on spline finite element method(FEM) and trimmed spline surfaces is proposed. For a new topology optimization based on spline FEM, trimming techniques are employed to represent arbitrary complex topologies in spline surfaces. Trimmed surface analysis which uses trimming information of trimmed surfaces for numerical analysis is employed to treat topologically complex spline surfaces. The boundaries are optimized by changing the positions of control points of the surface and those of trimming curves. As a reasonable measure for topological change, the selection criteria, which are defined by the ratio of the sensitivity on an objective function to that on a constraint, are employed for creation of a new inner front. Inner front merging algorithm is also implemented for high topological flexibility in the proposed approach. The proposed spline based topology optimization is demonstrated by applying some benchmarking problems.