The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

Critical role of bile acid (BA) in the cellular entry and permissiveness of Hepatitis B virus in vitro

Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.

단상 멀티레벨 능동전력필터를 위한 고조파 검지 기법 비교

김윤호(Yoon-Ho Kim),김수홍(Soo-Hong Kim),김성민(Sung-Min Kim),서강문(Kang-Moon Seo) 전력전자학회 2005 전력전자학술대회 논문집 Vol.- No.-

In this paper, harmonic detecting methods for the active power application are investigated. They are RDFT, Kalman Filter, Adaptive predictive filter, Instantaneous reactive power detecting method, Improved adaptive filter detecting method. The 5 harmonic detecting methods are simulated and their characteristics for the active filter application are compared using simulation results.

Floristic characteristics of vascular plants and first distributional report of Pseudostellaria baekdusanensis M. Kim in Yongneup wetland protected area

Young-Chul Kim,Hyun-Hee Chae,Sang-Heock Oh,Seung-Ho Choi2,Moon-Pyo Hong3,Gi-Heum Nam,Jae-Yoon Choi,Hyun-Sook Choi,Kyu-Song Lee 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.2

Yongneup wetland protected area, the only high moor in Korea, is a core area to conserve biodiversity. Even though the Yongneup wetland protected area is relatively small, various plant species are distributed in the Yongneup wetland protected area because it includes various habitats showing different environmental gradients. Vascular plants distributed in the Yongneup wetland protected area were identified as a total of 376 taxa that is composed of 73 families, 217 genera, 322 species, 3 subspecies, 44 varieties and 7 forms. For endangered plants designated by the Ministry of Environment, 5 species including Trientalis europaea var. arctica, Lilium dauricum, Halenia corniculata, Lychnis wilfordii and Menyanthes trifoliata were found and 34 taxa were confirmed to be distributed only in the mountainous wetland habitats. Regarding naturalized plants, a total of 11 taxa were distributed, but most of them were distributed in the areas where artificial interference has occurred. And in areas inside the wetlands that are relatively well preserved, 2 species of Bidens frondosa and Erigeron annuus were observed. In this study, the occurrence and distribution of Pseudostellaria baekdusanensis M. Kim, which was recently found in Mt. Baekdu and reported as a new species, were identified in the Yongneup wetland protected area. A wetland is a very vulnerable area to drastic environmental changes and damages to its ecosystem could cause the extinction of rare plant species which are distributed only in the wetlands. Therefore, it is mandatory that current status of the Yongneup wetland protected area is evaluated and actions to prevent rapid environmental changes are taken. Fourteen separate investigations were conducted in 2013 and another four in 2014, to evaluate current status of the Yongneup wetland protected area. These investigations have provided us the basic information for future actions of conservation and restoration.

Ergonomic Evaluation of Active Forward Collision Avoidance System Based on Required Deceleration Concept

Hong-Jun Yoon,Ji-Yong Lee,Woon-Sung Lee 대한인간공학회 2012 대한인간공학회 학술대회논문집 Vol.2012 No.11

Objective: The aim of this study is to conduct the ergonomic evaluation of AFCAS(Active Forward Collision Avoidance System) based on required deceleration. It is also to develop a low-cost AFCAS that uses a typical CCD camera. Background: This System detects vehicle and calculates distance from preceding vehicle and TTC(Time to Collision) to monitor and analyze accidental risk of a driver. Conventional system can cause suddenly brake depending on TTC. Accordingly, the driver could get a shock or discomfort. In order to minimize the discomfort of driver, the AFCAS was applied to the concept of required deceleration. Method: To evaluate the performance and discomfort AFCAS, driving simulator experiment was conducted. To evaluate performance, we used the following four performance : Distance from preceding vehicle when stopped, Intervention point of AFCAS, Deceleration of comparing AFCAS and Conventional system, Conflict with the preceding vehicle. And to evaluate discomfort, we used the following three parameters : RMS(Root-Mean-Square) of longitudinal deceleration, Participant’s Subjective uncomfortable index using Lickertis scale criterion, change rate of physiological state. Results: Experimental result of performance and discomfort evaluation showed that AFCAS is better than conventional system in all respects. And through a statistical analysis, the significance of measured variable was confirmed. Conclusion: AFCAS based on required deceleration performed better than conventional system and reduced discomfort during braking.

Comparison of Gefitinib and Erlotinib in Patients with Squamous Cell Lung Cancer (초)

Hong Joon Shin,Tae Ok Kim,Hyun Wook Kang,Yoon Hee Kim,Choel Kyu Park,Eun Young Kim,Su Young Chi,Hee Jung Ban,Yong Soo Kwon,In Jae Oh,Kyu Sik Kim,Sung Chul Lim,Young Chul Kim 대한결핵 및 호흡기학회 2010 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.110 No.-

Clinical Characteristics of Primary Peritoneal Carcinoma

Sang Young Roh,Sook Hee Hong,Yoon Ho Ko,Tae Hee Kim,Myung Ah Lee,Byoung Yong Shim,Jae Ho Byun,In Sook Woo,강진형,Young Seon Hong,Kyung Shik Lee 대한암학회 2007 Cancer Research and Treatment Vol.39 No.2

Purpose: The goal of this study was to determine the clinical and therapeutic characteristics of women with a primary peritoneal carcinoma (PPC).Materials and Methods: A retrospective clinical study was conducted to evaluate 22 women diagnosed with a PPC from 1993 to 2007 at the Hospitals of The Catholic University of Korea. Diagnoses were based on the Gynecologic Oncology Group criteria and clinical data. We collected patient clinicopathological data including age, presenting symptoms, pretreatment CA-125 values (U/ml), clinical stage (based on the FIGO stage), performance status (using the Eastern Cooperative Oncology Group scale), whether cytoreductive surgery was optimal or not, types of chemotherapy and response to treatment. We evaluated the clinical characteristics and response to treatment, time to treatment failure and overall survival.Results: The median overall survival of all patients was 23.1 months. The estimated 3-year survival rate was 29% (SE, 13%). The response rate to first-line platinum-based chemotherapy was 79% and the median time to treatment failure was 9.9 months (95% confidence interval, 1.38~18.4 months). By univariate and multivariate analysis, performance status was the only significant factor associated with overall survival (p〈0.05).Conclusion: We evaluated the clinical characteristics and treatment response of patients with a primary peritoneal carcinoma. Our results showed that it is possible to achieve long-term survival in patients with PPC. A further clinical study is to need to establish clinical characteristics and treatment outcomes. (Cancer Res Treat. 2007;39:65-68)

Clinical Characteristics of Ovarian Metastasis in Patients Who Underwent Curative Resection for Gastric Cancer

Hong Man Yoon,Young-Woo Kim,Myong Cheol Lim,Jun Ho Lee,Keun Won Ryu,Bang Ul Eom,Ji Yeon Park,Sang Yoon Park 대한종양외과학회 2011 대한임상종양학회 학술대회지 Vol.2011 No.9

Hydroxyl Radical Generation on Bubble Surface of Aqua-Plasma Discharge

Sung-Young Yoon,Sang-Heun Lee,Jung Wha Hong,Woo Seok Kang,Youn-Chang Jang,Gon-Ho Kim IEEE 2011 IEEE transactions on plasma science Vol.39 No.11

<P>The location of hydroxyl radical (OH<SUP>*</SUP>) generation in aqua plasma is investigated with the images of vapor bubble discharge in a conductive electrolyte (saline). A comparison of the visible-and UV-ray data taken from the Na<SUP>+*</SUP> and radical OH<SUP>*</SUP> reveals that Na<SUP>+*</SUP> is generated in both volume and surface of bubble while most of OH<SUP>*</SUP> is observed at the vapor surface. This result implies that the steamer-type of aqua plasma was generated in the vapor bubble covered over a metal electrode tip, and the charged particles disperse along the surface of the vapor bubble.</P>

The Effect of Pretreatment for Cemented Carbide Substrate Using Wet Blasting

Hong Sung-Pill,Kim Soo-Hyun,Kang Jae-Hoon,Yoon Yeo-Kyun,Kim Hak-Kyu 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

The pretreatment for substrate was carried out in change of gun pressure of bar using wet blasting. The size of powder was about . As the results, the surface roughness of cemented carbide substrate was improved with increment of gun pressure of wet blasting. A new surface layer was formed and Co particles were uniformly distributed over the entire surface after pretreatment. The adhesion of the pretreated substrate in same PVD-TiAlN film was improved and in approximately shown the best adhesion value.