Anti-inflammatory effect of hispidin on LPS induced macrophage inflammation through MAPK and JAK1/STAT3 signaling pathways

Han Ying-Hao,Chen Dong-Qin,Jin Mei-Hua,Jin Ying-Hua,Li Jing,Shen Gui-Nan,Li Wei-Long,Gong Yi-Xi,Mao Ying-Ying,Xie Dan-Ping,Lee Dong-Seok,Yu Li-Yun,Kim Sun-Uk,김지수,권태호,Cui Yu-Dong,Sun Hu-Nan 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

Severe inflammatory reactions caused by macrophage activation can trigger a systemic immune response. In the present study, we observed the anti-inflammatory properties of hispidin on LPS induced RAW264.7 macrophage cells. Our results showed that hispidin treatment significantly reduced the production of cellular NO, IL-6 and reactive oxygen species (ROS) while has not inhibitory effect on TNF-α productions. Excitingly, hispidin treatment retains the phagocytosis ability of macrophages which enabling them to perform the function of removing foreign invaders. Signaling studies showed, hispidin treatment dramatic suppressed the LPS induced mitogen activated protein kinases (MAPK) and JAK/STAT activations. In conclusion, our findings suggest that hispidin may be a new therapeutic target for clinical treatment of macrophages-mediated inflammatory responses.

RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Han Ying-Hao,Mao Ying-Ying,Yu Nan-Nan,Jin Mei-Hua,Jin Ying-Hua,Wang Ai-Guo,Zhang Yong-Qing,Shen Gui-Nan,Cui Yu-Dong,Yu Li-Yun,Lee Dong-Seok,Jo Yu-Jin,Sun Hu-Nan,Kwon Jeongwoo,권태호 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII+/+ (WT) and PrxII−/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII−/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII−/− vs. PrxII+/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

A study on the Ningbo-Zhoushan Port to Improve Korea-China Logistics Efficiency

Ying-yue Cui(최영열),Jae-Wook Lim(임재욱) 한국무역학회 2023 貿易學會誌 Vol.48 No.6

Ningbo-Zhoushan Port, located very close to the Yangtze River Delta, the most dynamic economic region in China, has a unique geographical advantage, but its economic development is very uncoordinated, however. In spite of it’s good and deep-water environments, the development speed is relatively backward due to the several reasons. In this paper, we analyze the current logistics situation of the Port of Ningbo-Zhoushan and the conditions for its development. We can identify the key factors using AHP analysis and suggest some strategies for the development of the port logistics by SWOT analysis. The regional logistics centering on Ningbo- Zhoushan port should be carried out first, and the efficiency of regional logistics should be maximized to improve the network and the function of logistics points of branch ports. It is necessary to integrate port, to accelerate the construction of hinterland cities and port logistics core areas, to gather resources from all over the country, and to expand the brand influence of the port. Logistics cooperation and strengthen exchanges with inland economic areas, expanding the ports influence to the inland and building a sustainable port system is also needed.

Numerical study on Wells turbine with penetrating blade tip treatments for wave energy conversion

Ying Cui,현범수 대한조선학회 2016 International Journal of Naval Architecture and Oc Vol.8 No.5

In order to optimize the performance of a Wells turbine with fixed guide vanes, the designs of an end plate and a ring on the tip of the turbine rotor are proposed as penetrating blade tip treatments. In this study, numerical investigations are made using computational fluid dynamics (CFD)-based ANSYS Fluent software, and validated by corresponding experimental data. The flow fields are analyzed and non-dimensional coefficients CA, CT and h are calculated under steady-state conditions. Numerical results show that the stalling phenomenon on a ring-type Wells turbine occurs at a flow coefficient of f ¼ 0.36, and its peak efficiency can reach 0.54, which is 16% higher than that of an unmodified turbine and 9% higher than in the case of an endplate-type turbine. In addition, quasi-steady analysis is used to calculate the mean efficiency and output work of a wave cycle under sinusoidal flow conditions. As a result, it has been found that the ring-type turbine is superior to other types of Wells turbines.

The Goal of Advertisement Translation: With Reference to C-E/E-C Advertisements

Ying Cui 세종대학교 언어연구소 2009 Journal of Universal Language Vol.10 No.2

Advertisements have a clear goal to promote a product or service by appealing to the audience, so the conditions of target readers must be considered. Thus, there are various restrictions for advertising, including people’s habits and customs, religion and beliefs, morals and ethics, laws in the target place, and linguistic or stylistic requirement. A translated advertisement is supposed to promote a product or service in the target culture. In this sense Skopos theory can be applied, for it pays great attention to purpose of translation, and holds that, as long as the purpose of translation can be realized, the translation is successful. Guided by this theory, we will summarize five points to be considered in advertisement translation. First, there is no need to stick to linguistic equivalence, for the dominant rule is the skopos rule. Second, the translated advertisements should be both textually and intertextually coherent in order that the readers can understand and trust them. Third, habits and customs, religion and beliefs, morals and ethics of the target audience should be considered and respected. Fourth, the relevant laws and regulations in the target culture should be observed. Fifth, the stylistic requirement of advertisements should be met.

Malignant Rhabdoid Tumor of the Kidney Combined with Multicystic Dysplasia in a 5-year-old Child

Ying Cui,엄민섭,정순희,김광진,정우희 대한의학회 2010 Journal of Korean medical science Vol.25 No.5

Multicystic dysplastic kidney (MCDK) is a relatively common developmental anomaly in infants and children and has a good prognosis. In contrast, a malignant rhabdoid tumor of the kidney (MRTK) is one of the most lethal neoplasms of early life. However,the presentation of such a lethal tumor combined with multicystic dysplasia has not been reported to date. In this report, we describe a case of MRTK in a 5-yr-old girl who also had multicystic dysplasia. She was previously diagnosed with MCDK at birth due to a huge palpable mass on the right side of the abdomen. The right kidney was extensively replaced by numerous grossly dilated, variable-sized cysts. Microscopically,the tumor cells show a diffusely infiltrative growth pattern, which revealed large non-cohesive, round-to-polygonal tumor cells with vesicular nuclei. Some tumor cells had eccentric nuclei and large, round, eosinophilic cytoplasmic inclusions. There were metanephrons present, with the central ureteric bud and peripheral branches surrounded by condensing mesenchyma, immature glomeruli, and metaplastic cartilage in the adjacent parenchyma. To our knowledge, this is the first combined case of the two aforementioned diseases and this case may, in fact, suggest a new disease entity.

Numerical study on Wells turbine with penetrating blade tip treatments for wave energy conversion

Cui, Ying,Hyun, Beom-Soo The Society of Naval Architects of Korea 2016 International Journal of Naval Architecture and Oc Vol.8 No.5

In order to optimize the performance of a Wells turbine with fixed guide vanes, the designs of an end plate and a ring on the tip of the turbine rotor are proposed as penetrating blade tip treatments. In this study, numerical investigations are made using computational fluid dynamics (CFD)-based ANSYS Fluent software, and validated by corresponding experimental data. The flow fields are analyzed and non-dimensional coefficients $C_A$, $C_T$ and ${\eta}$ are calculated under steady-state conditions. Numerical results show that the stalling phenomenon on a ring-type Wells turbine occurs at a flow coefficient of ${\phi}=0.36$, and its peak efficiency can reach 0.54, which is 16% higher than that of an unmodified turbine and 9% higher than in the case of an endplate-type turbine. In addition, quasi-steady analysis is used to calculate the mean efficiency and output work of a wave cycle under sinusoidal flow conditions. As a result, it has been found that the ring-type turbine is superior to other types of Wells turbines.

Upregulation of PITX2 Promotes Letrozole Resistance Via Transcriptional Activation of IFITM1 Signaling in Breast Cancer Cells

Ying-ying Xu,Hai-ru Yu,Jia-yi Sun,Zhao Zhao,Shuang Li,Xin-feng Zhang,Zhi-xuan Liao,Ming-ke Cui,Juan Li,Chan Li,Qiang Zhang 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2

Purpose Although the interferon  (IFN) signaling and the paired-like homeodomain transcription factor 2 (PITX2) have both been implicated in the progression of breast cancer (BCa), it remains obscure whether these two pathways act in a coordinated manner. We therefore aimed to elucidate the expression and function of PITX2 during the pathogenesis of endocrine resistance in BCa. Materials and Methods PITX2 expression was assessed in BCa tissues using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunohistochemistry and in experimentally induced letrozole-resistant BCa cells using RT-qPCR and immunoblotting. Effects of PITX2 deregulation on BCa progression was determined by assessing MTT, apoptosis and xenograft model. Finally, using multiple assays, the transcriptional regulation of interferon-inducible transmembrane protein 1 (IFITM1) by PITX2 was studied at both molecular and functional levels. Results PITX2 expression was induced in letrozole-resistant BCa tissues and cells, and PITX2 induction by IFN signaling powerfully protected BCa cells against letrozole insult and potentiated letrozole-resistance. Mechanistically, PITX2 enhanced IFN-induced AKT activation by transactivating the transcription of IFITM1, thus rendering BCa cells unresponsive to letrozoleelicited cell death. Additionally, ablation of IFITM1 expression using siRNA substantially abolished IFN-elicited AKT phosphorylation, even in the presence of PITX2 overexpression, thus sensitizing BCa cells to letrozole treatment. Conclusion These results demonstrate that constitutive upregulation of PITX2/IFITM1 cascade is an intrinsic adaptive mechanism during the pathogenesis of letrozole-resistance, and modulation of PITX2/IFITM1 level using different genetic and pharmacological means would thus have a novel therapeutic potential against letrozole resistance in BCa.

Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea

Cui, Ying-Shun,Im, Wan-Taek,Yin, Cheng-Ri,Lee, Jung-Sook,Lee, Keun Chul,Lee, Sung-Taik Microbiology Society 2007 International journal of systematic and evolutiona Vol.57 No.4

An Orbital Design Method for Satellite Formation Flying

Cui Hai-Ying,Li Jun-Feng,Gao Yun-Feng The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.2

An orbital design method of the formation initialization based on the relative orbital element method is presented. It firstly constructed the relative motion equation of the satellite formation flying in terms of the leader and followers' orbital elements. Then the equation was simplified when the orbit eccentricity of the leader satellite was small. And according to the satellites' mission, a general design method for the relative trajectory was proposed. The advantage of this method is that one can get a very simple analytical formula of each follower satellite's orbital elements when the orbital eccentricity of the leader satellite is zero. The simulation results show that the method is effective.