Inhalation of panaxadiol alleviates lung infl ammation via inhibiting TNFA/ TNFAR and IL7/IL7R signaling between macrophages and epithelial cells

Yifan Wang,Hao Wei,Zhen Song,Liqun Jiang,Mi Zhang,Xiao Lu,Wei Li,Yuqing Zhao,Lei Wu,Shuxian Li,Huijuan Shen,Qiang Shu,Yicheng Xie 고려인삼학회 2024 Journal of Ginseng Research Vol.48 No.1

Background: Lung inflammation occurs in many lung diseases, but has limited effective therapeutics. Ginseng andits derivatives have anti-inflammatory effects, but their unstable physicochemical and metabolic propertieshinder their application in the treatment. Panaxadiol (PD) is a stable saponin among ginsenosides. Inhalationadministration may solve these issues, and the specific mechanism of action needs to be studied. Methods: A mouse model of lung inflammation induced by lipopolysaccharide (LPS), an in vitro macrophageinflammation model, and a coculture model of epithelial cells and macrophages were used to study the effectsand mechanisms of inhalation delivery of PD. Pathology and molecular assessments were used to evaluate efficacy. Transcriptome sequencing was used to screen the mechanism and target. Finally, the efficacy andmechanism were verified in a human BALF cell model. Results: Inhaled PD reduced LPS-induced lung inflammation in mice in a dose-dependent manner, includinginflammatory cell infiltration, lung tissue pathology, and inflammatory factor expression. Meanwhile, the dose ofinhalation was much lower than that of intragastric administration under the same therapeutic effect, which maybe related to its higher bioavailability and superior pharmacokinetic parameters. Using transcriptome analysisand verification by a coculture model of macrophage and epithelial cells, we found that PD may act by inhibitingTNFA/TNFAR and IL7/IL7R signaling to reduce macrophage inflammatory factor-induced epithelial apoptosisand promote proliferation. Conclusion: PD inhalation alleviates lung inflammation and pathology by inhibiting TNFA/TNFAR and IL7/IL7Rsignaling between macrophages and epithelial cells. PD may be a novel drug for the clinical treatment of lunginflammation.

Poly(3,4-ethylenedioxythiophene)-Coated Conductive Polyester Non-woven Fabric Prepared by Enzymatic Polymerization

Yifan Cui,Zhe Jiang,Yu Zhou,Qiang Wang,Man Zhou,Ping Wang,Yuanyuan Yu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.6

Conductive polyester non-woven fabric was prepared using horseradish peroxidase (HRP) catalytic agent, 3,4-ethylenedioxythiophene monomer and poly(p-styrenesulfonic acid) dopant. The electrical resistance of the preparedconductive polyester non-woven fabric was reduced to 1.3×104 Ω from 1014 Ω of the raw polyester non-woven fabric becauseof the addition of poly(3,4-ethylenedioxythiophene) the conductivity of conductive polyester non-woven fabric decreased byabout 60 % after 300 bending cycles. Notably, the temperature of the conductive polyester non-woven fabric increased by73.1 °C after 150 s of light irradiation from a lamp source, while that of the raw polyester non-woven fabric increased by only19.4 °C under the same conditions.

Trisodium citrate-assisted synthesis of BiOBr nanostructure catalyst for efficient activity under visible light

Weiming Zhou,Yifan Jiang,Shichang Sun,Mingxin Zhang,Ibrahim Lawan,Gerard Franklyn Fernando,Liwei Wang,Zhanhui Yuan 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.2

A simple one step wet-chemical method assisted by trisodium citrate was employed in the synthesis of BiOBr applied as a photocatalyst. Photocatalytic activity of the BiOBr was investigated for the degradation of Rhodamine B (RhB) dye under visible light irradiation (>420 nm). The results indicated that trisodium citrate is more favorable to the formation of hierarchical architectures and reduces the particle size of BiOBr photocatalyst. BiOBr with hierarchical architectures exhibiting significantly higher catalytic activity than that with ordinary nanostructure. The significant improvement could be attributed to the high specific surface area (24.14m2·g1), average pore sizes (34.09 nm) and average pore volume (0.24 cm3·g1).

Fault Detection for a Class of Closed-loop Hypersonic Vehicle System via Hypothesis Test Method

Xunhong Lv,Yifan Fang,Zehui Mao,Bin Jiang,Ruiyun Qi 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.1

This paper studies the fault detection problem for a class of hypersonic vehicle with actuator faults,disturbances and random noises. To handle the unknown disturbances, an unknown input Kalman filter (UIKF) ispresented to estimate the unknown system states and disturbances, simultaneously. Considering that the closed-loopstructure brings the robustness to the hypersonic vehicle, which could cover some faults, the Total Measurable FaultInformation Residual (ToMFIR) is employed as the fault detection residual. Moreover, to deal with the randomnoises, the hypothesis testing method is utilized to obtain the thresholds under some fault detection performances(false alarm rate and missing alarm rate). The fault detectability condition is also derived. Finally, the simulationsverify the effectiveness of the proposed fault detection method

Corrosion behavior of biodegradable magnesium alloy by MAF treatment

Xinzhe Gao,Yifan Yang,Bi Ying Shi,Chun Yu Dai,ChuanYao Zhai,Bing Cheng Zhao,Heng Bo Jiang 대한치과재료학회 2021 대한치과기재학회 학술대회 Vol.2021 No.4

Introduction. Magnesium and magnesium alloys, with mismatched degradation rates and products that are expected to support the effects being issues that need to be overcome, are hindered from clinical application. The coating preparation plays an active role in tackling this problem. The purpose of this work is to observe the corrosion control by the MAF coating in vitro and in vivo. Method. The MgF2 layer was prepared on the surface of AZ31 magnesium alloy by MAF method at 190 V DC in HF·H₂O (46%) solution. The magnesium alloy samples before and after the treatment were compared by scanning electron microscopy (SEM) observation. The coating structure and constituents were confirmed using cross-sectional SEM, energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) analysis. Potentiodynamic polarization (PDP) was utilized for electrochemical evaluation of corrosion potential and current density. 6-week-old male rats were used as experimental subjects for in vivo experiments. The volume changes and surface morphology of the corroded samples were dynamically monitored using micro-CT for 16 weeks. Results. The distribution of coating elements observed by EDS is dominated by magnesium and fluorine. The XRD analysis shows that the prepared coatings are mainly MgF2 crystals. The coating was observed by SEM to be uniformly dense and coral-like. The results of the PDP experiments showed that the coated samples had lower corrosion current density and corrosion potential. Comparison of the two groups of in vivo experiments revealed that the rate of volume change decreased in the coated samples and the density change during micro-CT observation was less than that of the uncoated group. Conclusions. Effective coatings were prepared on the surface of AZ31 magnesium alloy using the MAF method. This coating was also validated the potential to control the corrosion rate and corrosion process of magnesium alloy by both in vitro and in vivo evaluation systems.

Cell-Penetrating Peptide-Modified PLGA Nanoparticles for Enhanced Nose-to-Brain Macromolecular Delivery

Lu Yan,Huiyuan Wang,Yifan Jiang,Jinhua Liu,Zhao Wang,Yongxin Yang,Shengwu Huang,Yongzhuo Huang 한국고분자학회 2013 Macromolecular Research Vol.21 No.4

Macromolecular drugs become an essential part in neuroprotective treatment. However, the nature of ineffective delivery crossing the blood brain barrier (BBB) renders those macromolecules undruggable for clinical practice. Recently, brain target via intranasal delivery have provided a promising solution to circumventing the BBB. Despite the direct route from nose to brain (i.e. olfactory pathway), there still are big challenges for large compounds like proteins to overcome the multiple delivery barriers such as nasal mucosa penetration, intracellular transport along the olfactory neuron, and diffusion across the heterogeneous brain compartments. Herein presented is an intranasal strategy mediated by cell-penetrating peptide modified poly(lactic-co-glycolic acid) (PLGA) nanoparticles for the delivery of insulin to the brain, a potent therapeutic against Alzheimer’s disease. The results revealed that the cell-penetrating peptide can potentially deliver insulin into brain via the nasal route, showing a total brain delivery efficiency of 6%. It could serve as a potential treatment for neurodegenerative diseases.

Manganese induces neuroinflammation via NF-κB/ROS NLRP3 pathway in rat brain striatum and HAPI cells

Xinyuan Zhao,Lifeng Yin,Yifan Wu,Muxi Han,Yin Zhuang,Yewen Cong,Yiming Liu,Gang Chen,Junkang Jiang 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.2

Backgrounds: Chronic exposure to excessive Mn can result in neurodegenerative symptoms, whose precise molecular mechanism remains largely unclear. Here, we measured the role and mechanism of NLRP3 in Mninduced neuroinflammation in vivo and vitro. Methods: The effects of Mn on NLRP3 activation were investigated by Westernblot, IHC, immunofluorescence analysis, as well as ELISA. We assessed NF-κB activation through measurement of phosphorylation and nuclear translocation. The mechanisms bywhich Mn induced NLRP3 activation were assessed by specific inhibitors. Results: We found that Mn exposure facilitated the activation of NLRP3 inflammasome to promote the production of IL-1β and IL-18 in dose- and time-dependent manners in HAPI cells. In addition, the NLRP3 inflammasome was also dramatically activated in microglia of rat brain striatum after Mn exposure. We also found increased ROS and NF-κB activation. Notably, the activation of NLRP3 was significantly attenuated by pretreatment with NF-κB and ROS inhibitors. Conclusion: These findings suggest that NLRP3 activation plays an important role in Mn-induced neuroinflammation, and it is associated with NF-κB and ROS.

Effects of Sulfur Introduction on the UV and the Visible Emission Properties of ZnO

Hongying Guo,Feihong Jiang,Run Yuan,Jun Zhang,Yuanping Sun,Yifan Liu,Yongxin Qiu,Taofei Zhou,Xionghui Zeng,Baoshun Zhang,Ke Xu,Hui Yang 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.4

Hexagonal ZnO particles have been synthesized by using the hydrothermal method with differentsulfur concentrations in the reaction solutions. Structural and optical characterizations have beenconducted to study the effects of the sulfur in the reaction solutions on the properties of thesynthesized ZnO in the ultraviolet (UV) and the visible (VIS) bands. The existance of sulfur inthe solutions can help to introduce compression strain along the a axis and an opposite trend forthe parameter c with strain inside the formed ZnO particles, which means the total strain in thesamples is presented along the c axis. The average size of the ZnO particles, as calculated fromSEM images, shows the same trend as the strain in the samples. The increasing incorporation ofsulfur causes an increase in the VIS luminescence band, which can be attributed to an increase inthe number of sulfur-induced defects.

Inhibition of BETs prevents heat shock-induced cell death via upregulating HSPs in SV40 large T antigen transfected cells

Zhou Nan,Zhang Ye,Lei Gongyun,Chen Yifan,Lin Ting,Liu Qin,Zhao Yinshuang,Mao Jiahui,Jiang Yongying,Mao Renfang 한국유전학회 2022 Genes & Genomics Vol.44 No.10

Background: Heat shock response is a protected mechanism against environmental changes for the organism, which must be tightly regulated. Bromodomain and extra terminal-containing protein family (BETs) regulate numerous gene expression in many physiological and pathological conditions, including viral infection. SV40 is considered as a highly human disease-associated virus. Objective: We aimed to explore whether BETs play a role in heat shock in SV40 large T antigen transfected cells. Methods: SV40LTA was transfected in HeLa cells using the Lipofectamine 8000. BETs inhibitor JQ1 and I-BET-762 was employed to treat transfected cells and HEK-293 T cells. Heat shock treatment was performed to determine the effect of JQ1 and I-BET-762 on these cells. Western blot and quantitative RT-PCR were carried out to assess the expression of HSP70 and other HSPs. Results: We found that inhibition of BETs by JQ1 and I-BET-762 protects cells from heat shock-induced death in HEK293T cells. Both JQ1 and I-BET-762 induce the expression of HSPs and HSF1 in HEK-293 T cells. However, neither JQ1 nor I-BET-762 fail to induce the expression of HSPs in either HeLa or HBL-1 cells. When SV40 large T antigen was transfected into HeLa cells, the induction of HSP70 expressing and the protection of heat shock-induced cell death are reproduced by JQ1 and IBET treatment in these transfected cells. Conclusions: Inhibition of BETs by JQ1 and I-BET-762 prevents heat shock-induced cell death via upregulating HSPs in SV40 large T antigen transfected cells. Our data indicate a novel function of BETs in SV40 large T antigen transformed cells, affecting HSPs and HSF1 as well as its function on heat shock response.

Process optimization and synthesis of lanthanum-cobalt perovskite type nanoparticles (LaCoO3) prepared by modified proteic method: Application of response surface methodology

Zaharaddeen N. Garba,Wei Xiao,Weiming Zhou,Ibrahim Lawan,Yifan Jiang,Mingxi Zhang,Zhanhui Yuan 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.11

Due to increasing interest in the application of perovskites as promising adsorbents, the present study looks at how central composite design (CCD), a subset of response surface methodology (RSM), can statistically play a role in producing optimum lanthanum oxide-cobalt perovskite type nanoparticles (LaCoO3) by using a modified proteic synthesis method. The optimum LaCoO3 produced was tested for its capability in removing methyl orange (MO) and rhodamine B (RhB) dyes from aqueous solution. Calcination temperature and calcination time were optimized with the responses being percentage yield, MO and RhB removal. The best temperature and calcination time obtained were 775 oC and 62mins, respectively, giving good and appreciable values for the three responses. The resulting optimal LaCoO3 was characterized by Fourier transform infra-red (FTIR), ultraviolet-visible spectrophotometry (UV/vis), scanning electron microscopy (SEM), pH of zero point charge (pHpzc) as well as BET analysis, yielding a mesoporous adsorbent with surface area of 61.130m2 g−1 as well as 223.55 and 239.45mg g−1 as the monolayer adsorption capacity values for MO and RhB, respectively. Freundlich model was the best in describing the equilibrium adsorption data with respect to both MO and RhB with the kinetic data for the two dyes both obeying pseudo-second-order kinetics model.