A novel method for identifying rotor-stator rubbing positions using the cepstrum analysis technique

Guo Chen,Yong-Quan Liu,Guang-Yi Jiang,Cheng-Gang Li,Guo-Quan Feng,De-You Wang 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.9

A novel method of aero-engine rubbing positions identification based on cepstrum analysis is proposed, and the transfer path characteristicswhich reflect the transfer characteristics information from rubbing points to casing measuring points are separated from the vibrationacceleration signals of casing by means of cepstrum analysis. Therefore, there is different transfer characteristics information atdifferent rubbing positions, and in view of this, twenty rubbing positions identification features from the cepstrum are extracted. A largenumber of rubbing experiments of different positions are simulated with the rotor experiment rig of aero-engine, and the characteristicanalysis of experimental samples at different rubbing positions is carried out, and the results indicate the consistency of features to thesame rubbing position and the difference of the features to the different rubbing positions. Finally, the aero-engine rubbing positionsidentification is carried out using the nearest neighbor classification method, the recognition rate reaches 100%, and the effectiveness ofthe method is full verified.

Metagenomic Insight into Lignocellulose Degradation of the Thermophilic Microbial Consortium TMC7

( Yi Wang ),( Chen Wang ),( Yonglun Chen ),( Beibei Chen ),( Peng Guo ),( Zongjun Cui ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

Biodegradation is the key process involved in natural lignocellulose biotransformation and utilization. Microbial consortia represent promising candidates for applications in lignocellulose conversion strategies for biofuel production; however, cooperation among the enzymes and the labor division of microbes in the microbial consortia remains unclear. In this study, metagenomic analysis was performed to reveal the community structure and extremozyme systems of a lignocellulolytic microbial consortium, TMC7. The taxonomic affiliation of TMC7 metagenome included members of the genera Ruminiclostridium (42.85%), Thermoanaerobacterium (18.41%), Geobacillus (10.44%), unclassified_f__Bacillaceae (7.48%), Aeribacillus (2.65%), Symbiobacterium (2.47%), Desulfotomaculum (2.33%), Caldibacillus (1.56%), Clostridium (1.26%), and others (10.55%). The carbohydrate-active enzyme annotation revealed that TMC7 encoded a broad array of enzymes responsible for cellulose and hemicellulose degradation. Ten glycoside hydrolases (GHs) endoglucanase, 4 GHs exoglucanase, and 6 GHs β-glucosidase were identified for cellulose degradation; 6 GHs endo-β-1,4-xylanase, 9 GHs β-xylosidase, and 3 GHs β-mannanase were identified for degradation of the hemicellulose main chain; 6 GHs arabinofuranosidase, 2 GHs α-mannosidase, 11 GHs galactosidase, 3 GHs α-rhamnosidase, and 4 GHs α-fucosidase were identified as xylan debranching enzymes. Furthermore, by introducing a factor named as the contribution coefficient, we found that Ruminiclostridium and Thermoanaerobacterium may be the dominant contributors, whereas Symbiobacterium and Desulfotomaculum may serve as “sugar cheaters” in lignocellulose degradation by TMC7. Our findings provide mechanistic profiles of an array of enzymes that degrade complex lignocellulosic biomass in the microbial consortium TMC7 and provide a promising approach for studying the potential contribution of microbes in microbial consortia.

Efficient degradation of imidacloprid in wastewater by a novel p-n heterogeneous Ag2O/BiVO4/diatomite composite under hydrogen peroxide

Jing Chen,Qifang Ren,Chunshan Xu,Bin Chen,Shaohua Chen,Yi Ding,Zhen Jin,Wanmi Guo,Xinyu Jia 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

In this study, Ag2O/BiVO4/diatomite composite with p-n heterojunction structure was synthesized bysimple hydrothermal method with diatomite as carrier. It was found that under the action of hydrogenperoxide (H2O2), the imidacloprid (IMD) could be effectively degraded by the composite. Systemic evaluationswas conducted on effects of pH value, catalyst dosage, H2O2 concentration, initial IMD concentration,and anion on how effeciently this new photocatalytic composite can degrade IMD. The results showthat the p-n heterojunction formed between the two contact surfaces of Ag2O nanoparticle and BiVO4promotes the charge transfer between the interfaces, inhibits recombination of electrons and holes,and thus significantly improves the catalytic performance. The stability and reusability of Ag2O/BiVO4/diatomite composites were tested with ICP experiment and cyclic experiment. Active substancecapture experiment and ESR reveal the photocatalytic reaction mechanism and confirm that superoxideradical (O2), hydroxyl radical (OH), and h+ can promote the degradation of IMD. The degradation productsand the intermediates of IMD were identified through LC-MS, on a basis of which the possible transformationpathway was proposed. In situ synthesis of p-type Ag2O and n-type BiVO4 heterojunctioncomposites has opened a new direction for photocatalytic degradation of organic pollutants.

Construction and Characterization of a Full-length cDNA Library and Identification of Genes Involved in Salinity Stress in Wild Eggplant (Solanum torvum Swartz)

Gang Chen,Hua Wang,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Qian-Qian Liu,Gong-Chen Zhang,Guo-Hu Chen 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2

The objectives of this paper were to construct a full-length cDNA library and to isolate genes that confer salt tolerance from the leaves of salinity-tolerant wild eggplant variety, ‘Torvum Vigor’ (Solanum torvum Swartz). A full-length cDNA library from the leaves was successfully constructed by a switching mechanism at 5’-end of RNA transcript (SMART) approach and a long-distance PCR (LD-PCR) technique. The titer of the primary cDNA library was 3.6 ×106 cfu・mL-1 and that of the amplified library was 1.2 × 1010 cfu・mL-1. Gel electrophoresis results showed that most of the cDNA inserts ranged from 0.40 to 2.5 kb, with a recombination rate of 99%. A total of 427 randomly selected positive clones were sequenced. After removing the unsuccessful reads, 364 datasets were obtained and have been submitted to the NCBI Nucleotide Sequence Database under GenBank accession numbers JK265131-JK265494. Among the 364 submitted sequences, 74.45% of them contained full-length coding regions. BLASTX analysis revealed that 62.36% of the ‘Torvum Vigor’ expressed sequence tags (ESTs) possessed homology to known or putative proteins of other organisms. Seven genes that might be responsible for the encoding of known proteins in other organisms were identified to confer salt tolerance. This evidence demonstrated that the cDNA library constructed was a full-length library of high quality. It could be a useful resource for further research in the cloning of stress-related genes, which could be utilized in the genetic improvement of vegetable crops using transgenic technology.

Inflammatory Endotypes and Tissue Remodeling Features in Antrochoanal Polyps

Chen Cai-Ling,Wang Yu-Ting,Yao Yin,Pan Li,Guo Bei,Zhu Ke-Zhang,Ma Jin,Wang Nan,Li Xue-Li,Deng Yi-Ke,Liu Zheng 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.6

Purpose: The pathogenic mechanisms of antrochoanal polyps (ACPs) remain largely unknown. This study aimed to characterize inflammatory patterns and tissue remodeling features in ACPs. Methods: Inflammatory cell infiltration and tissue edema severity as well as fibrin deposition in ACPs and bilateral eosinophilic and noneosinophilic nasal polyps (NPs) were studied with immunohistochemical and immunofluorescence staining. Cytokine levels in sinonasal tissues were detected with the Bio-Plex assay. The expression of coagulation and fibrinolytic markers was measured using reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assays. Results: Compared to control tissues and bilateral eosinophilic and noneosinophilic NPs, ACPs had higher levels of neutrophil infiltration and expression of myeloperoxidase (MPO), interleukin (IL)-8 and interferon (IFN)-γ. In total, 94.4% of ACPs demonstrated an eosinophil cationic protein/MPO ratio of < 1, compared to 79.0% of noneosinophilic and 26% of eosinophilic NPs. Principle component and multiple correspondence analyses revealed a neutrophilic and type 1 inflammation pattern in ACPs. Compared to control tissues, edema scores and fibrin deposition were increased, whereas d-dimer and tissue plasminogen activator (tPA) levels were decreased in ACPs and bilateral NPs, with more prominent changes in ACPs even than in eosinophilic NPs. The tPA levels were negatively correlated with IFN-γ, IL-8, and MPO levels in ACPs. Neutrophils were the major cellular source of IFN-γ in ACPs, and the number of IFN-γ+ neutrophils was elevated in ACPs than in control tissues and bilateral eosinophilic and noneosinophilic NPs. Conclusions: ACPs are characterized by the neutrophilic and type 1 inflammation endotype. Neutrophil-derived IFN-γ is associated with reduced tPA production in ACPs.

Research Progress of Central and Peripheral Corticotropin-Releasing Hormone in Irritable Bowel Syndrome with Comorbid Dysthymic Disorders

( Yi Feng Liang ),( Xiao Qi Chen ),( Meng Ting Zhang ),( He Yong Tang ),( Guo Ming Shen ) 대한소화기기능성질환·운동학회 2024 Gut and Liver Vol.18 No.3

Irritable bowel syndrome (IBS) is considered a stress disorder characterized by psychological and gastrointestinal dysfunction. IBS patients not only suffer from intestinal symptoms such as abdominal pain, diarrhea, or constipation but also, experience dysthymic disorders such as anxiety and depression. Studies have found that corticotropin-releasing hormone plays a key role in IBS with comorbid dysthymic disorders. Next, we will summarize the effects of corticotropin-releasing hormone from the central nervous system and periphery on IBS with comorbid dysthymic disorders and relevant treatments based on published literatures in recent years. (Gut Liver 2024;18:391-403)

Impact performance for high frequency hydraulic rock drill drifter with sleeve valve

Guo, Yong,Yang, Shu Yi,Liu, De Shun,Zhang, Long Yan,Chen, Jian Wen Korean Society for Fluid machinery 2016 International journal of fluid machinery and syste Vol.9 No.1

A high frequency hydraulic rock drill drifter with sleeve valve is developed to use on arm of excavator. In order to ensure optimal working parameters of impact system for the new hydraulic rock drill drifter controlled by sleeve valve, the performance test system is built using the arm and the hydraulic source of excavator. The evaluation indexes are gained through measurement of working pressure, supply oil flow and stress wave. The relations of working parameters to impact system performance are analyzed. The result demonstrates that the maximum impact energy of the drill drifter is 98.34J with impact frequency of 71HZ. Optimal pressure of YZ45 rock drill is 12.8 MPa-13.6MPa, in which the energy efficiency reaches above 58.6%, and feature moment of energy distribution is more than 0.650.

Robust Segmentation for Low Quality Cell Images from Blood and Bone Marrow

Chen Pan,Yi Fang,Xiang-guo Yan,Chong-xun Zheng 대한전기학회 2006 International Journal of Control, Automation, and Vol.4 No.5

Biomedical image is often complex. An applied image analysis system should deal with the images which are of quite low quality and are challenging to segment. This paper presents a framework for color cell image segmentation by learning and classification online. It is a robust two-stage scheme using kernel method and watershed transform. In first stage, a two-class SVM is employed to discriminate the pixels of object from background; where the SVM is trained on the data which has been analyzed using the mean shift procedure. A real-time training strategy is also developed for SVM. In second stage, as the post-processing, local watershed transform is used to separate clustering cells. Comparison with the SSF (Scale space filter) and classical watershed-based algorithm (those are often employed for cell image segmentation) is given. Experimental results demonstrate that the new method is more accurate and robust than compared methods.

Overexpression of StNHX1, a Novel Vacuolar Na+/H+ Antiporter Gene from Solanum torvum, Enhances Salt Tolerance in Transgenic Vegetable Soybean

Guo-Hu Chen,Wenyan Wang,Li-Fei Yang,Jun-Yi Gai,Yue-Lin Zhu 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.3

Soil salinity is the primary limiting factor affecting the growth and production of vegetable soybean[Glycine max (L.) Merrill] worldwide. Plants can survive in salinity conditions through the mechanism of sodium ion(Na+) compartmentalization, during which the vacuolar Na+/H+ antiporter (NHX) genes play a critical role. In the presentstudy, a novel vacuolar NHX gene from Solanum torvum Swartz ‘Torvum Vigor’ (StNHX1; GenBank accessionnumber: JN606860.1) was isolated and transferred into the vegetable soybean ‘Xinliaoxian’ through an Agrobacteriummediatedprotocol. The PCR and southern blot analyses confirmed the successful integration of the exogenous genes. The GUS assay and semi-quantitative RT-PCR analysis showed that the alien genes were inherited by the progeniesand that the StNHX1 gene was overexpressed in the T3 generation. The overexpression of this gene conferred hightolerance to salt stress. Under 100 mM NaCl conditions, the scorch scores and the Na+ and malondialdehyde (MDA)contents of the leaves of the transgenic lines were significantly lower as compared with the wild-type (WT) plants,while the K+/Na+ ratio, the contents of K+, relative chlorophyll and relative water, and some morphological traits of thetransgenic plants were significantly higher than those of the WT. These results indicate that the overexpression ofStNHX1 enhances the salt tolerance of vegetable soybean and that StNHX1 is one of the promising target genes thatcan be manipulated to improve the salinity tolerance of crops.

Microwave absorption and photocatalytic activity of MgxZn1−x ferrite/diatomite composites

Guo Wanmi,Wang Sulei,Ren Qifang,Jin Zhen,Ding Yi,Xiong Chun-Yu,Li Jinpeng,Chen Jing,Zhu Yuelei,오원춘 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.2

Mg x Zn 1−x ferrite/diatomite composites were synthesized by a two-step combination process (hydrothermal method and mechanical mixing method). The products were characterized by X-ray diff raction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR), UV–visible absorption spectrum (UV–Vis), X-ray photoelectron spectroscope (XPS), and microwave vector network analyzer. The photocatalytic results showed that the degradation rate of Rhodamine B (RhB) was 50–90% after 90 min of visible light. At 2–18 GHz frequency, when the Mg–Zn ratio increases to 0.8, the refl ection loss of a single MgxZn 1−x ferrite reaches − 7.23 dB at 8.75 GHz frequency in 3.5 mm thickness while the ratio is 0.2, the refl ection loss at 10.23 GHz reaches − 4.07 dB. The photocatalytic performance of Mgx Zn 1−x ferrite /diatomite composite for the degradation of RhB and its microwave absorption performance as absorbent are studied. The importance of developing new functional coatings to improve human environment is discussed.