STUDY ON DIGITAL SIMULATION FORMING TECHNOLOGY OF ETHNIC TRADITIONAL WEAVING TECHNOLOGY

Chunshan Deng,Tingyu Xu,Yue Xin,Longlin Zhang 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06

The aim of this research is to expand application areas of the advanced manufacturing concept and technology based on combining the traditional weaving techniques with digital forming technology. Through the man-machine interface of traditional weaving techniques of processing machines and craftsmanship complex data visualization and interactive operation, Virtual Reality Technology can realize computer model images, which is a kind of new form of artistic language and the key of VR art presenting its unique advantages. Combined with digital technology, the production of raw materials can generate 2D graphics, and 3D object can also be generated by using rapid solidification laser material preparation and forming technology (3-Dimension Printing). In one word, the traditional weaving techniques of processing machine and craftsmanship realized the complete inheritance. In this paper, main content are 4 parts including the analysis of ethnic traditional weaving technology in southwest areas of China, the usage of fashion digital simulation forming technology, the combination of traditional weaving technology with digital forming technology, and expanding of digital customized technology. We started our research work using grass linen and its weaving machine. In the end, the conclusion of study will be used to build the digital museum online for Southwest Ethnic Traditional Weaving Technology Museum, which is also bridging around world marketing for traditional culture.

Structural and Functional Analyses of Periplasmic 5′-Methylthioadenosine/<i>S</i>-Adenosylhomocysteine Nucleosidase from <i>Aeromonas hydrophila</i>

Xu, Yongbin,Wang, Lulu,Chen, Jinli,Zhao, Jing,Fan, Shengdi,Dong, Yuesheng,Ha, Nam-Chul,Quan, Chunshan American Chemical Society 2017 Biochemistry Vol.56 No.40

<P>The Gram-negative, rod-shaped bacterium Aeromonas hydrophila has two multifunctional 5/-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) enzymes, MtaN-1 and MtaN-2, that differ from those in other bacteria. These proteins are essential for several metabolic pathways, including biological methylation, polyamine biosynthesis, methionine recycling, and bacterial quorum sensing. To gain insight into how these two proteins function, we determined four high-resolution crystal structures of MtaN-1 in its apo form and in complex with the substrates S-adenosyl-L-homocysteine, S'-methylthioadenosine, and 5'-deoxyadenosine. We found that the domain structures were generally similar, although slight differences were evident. The crystal structure demonstrates that AhMtaN-1 has an extension of the binding pocket and revealed that a tryptophan in the active site (Trp199) may playa major role in substrate binding, unlike in other MTAN proteins. Mutation of the Trp199 residue completely abolished the enzyme activity. Trp199 was identified as an active site residue that is essential for catalysis. Furthermore, biochemical characterization of AhMtaN-1 and AhMtaN-2 demonstrated that AhMtaN-1 exhibits inherent trypsin resistance that is higher than that of AhMtaN-2. Additionally, the thermally unfolded AhMtaN-2 protein is capable of refolding into active forms, whereas the thermally unfolded AhMtaN-1 protein does not have this ability. Examining the different biochemical characteristics related to the functional roles of AhMtaN-1 and AhMtaN-2 would be interesting. Indeed, the biochemical characterization of these structural features would provide a structural basis for the design of new antibiotics against A. hydrophila.</P>

A Wrapper for Extracting Information Records from Forums based on Page Segmentation

Chunshan Li,Jingjing Chen,Dianhui Chu,Ge Song,Haijun Zhang,Yunming Ye,Jianliang Xu 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.4

Hexameric assembly of membrane fusion protein YknX of the sporulation delaying efflux pump from <i>Bacillus amyloliquefaciens</i>

Xu, Yongbin,Jo, Inseong,Wang, Lulu,Chen, Jinli,Fan, Shengdi,Dong, Yuesheng,Quan, Chunshan,Ha, Nam-Chul Elsevier 2017 Biochemical and biophysical research communication Vol.493 No.1

<P><B>Abstract</B></P> <P>Membrane fusion proteins (MFPs) play an essential role in the action of the drug efflux pumps and protein secretion systems in bacteria. The sporulation delaying protein (SDP) efflux pump YknWXYZ has been identified in diverse <I>Bacillus</I> species. The MFP YknX requires the ATP-binding cassette (ABC) transporter YknYZ and the Yip1 family protein YknW to form a functional complex. To date, the crystal structure, molecular function and mechanism of action of YknX remain unknown. In this study, to characterize the structural and biochemical roles of YknX in the functional assembly of YknWXYZ from <I>B. amyloliquefaciens</I>, we successfully obtained crystals of the YknX protein that diffracted X-rays to a resolution of 4.4 Å. We calculated an experimentally phased map using single-wavelength anomalous diffraction (SAD), revealing that YknX forms a hexameric assembly similar to that of MacA from Gram-negative bacteria. The hexameric assembly of YknX exhibited a funnel-like structure with a central channel and a conical mouth. Functional studies <I>in vitro</I> suggest that YknX can bind directly to peptidoglycan. Our study provides an improved understanding of the assembly of the YknWXYZ efflux pump and the role of YknX in the complex.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The functional and structural properties of the YknX from <I>B. amyloliquefaciens.</I> </LI> <LI> The three-dimensional structure of MFP YknX and it has a strong affinity toward peptidoglycan. </LI> <LI> The YknX may forms a hexameric assembly in the crystal packing. </LI> <LI> The YknX displayed a similar upside-down, funnel-shaped orientation as <I>Aa</I> MacA. </LI> </UL> </P>

Synergy of ionic liquid and confinement in the design of supported palladium catalyst for efficient selective hydrogenation of acetylene

Qingtao Wang,Yiqi Xu,Jing Zhou,Longyu Xu,Lu Yu,Dahao Jianga,Chunshan Lu,Zhiyan Pan,Qunfeng Zhang,Xiaonian Li 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-

We present here a novel fabrication of highly effective supported Pd catalysts (Pd@S-1@IL) derived fromPd@S-1 core–shell material coated with ionic liquids (ILs), [Prmim][Cl], for the selective hydrogenation ofacetylene. Catalytic testing on Pd@S-1@IL catalyst illustrated a remarkable selectivity towards semihydrogenation(over 90% for ethylene, irrespective of conversion level). Moreover, no indications ofdeactivation were observed after 800 h on stream even under relatively severe conditions. Detailedcharacterization indicated that the selectivity enhancement was ascribed to thefilter effect along withelectronic effect on the adsorption/desorption processes arising from the ionic liquids layer. The stability,which has ranked the top reported values over advanced Pd-based catalysts, is attributed to theconfinement effect of Pd NPs within the supports thus restricting the migration of metals. The fabricationof core–shell architecture combined with the advantages of ionic liquid layer offers a promising guidelinefor efficient catalytic transfer of chemical resource.

Efficient degradation of imidacloprid in wastewater by a novel p-n heterogeneous Ag2O/BiVO4/diatomite composite under hydrogen peroxide

Jing Chen,Qifang Ren,Chunshan Xu,Bin Chen,Shaohua Chen,Yi Ding,Zhen Jin,Wanmi Guo,Xinyu Jia 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

In this study, Ag2O/BiVO4/diatomite composite with p-n heterojunction structure was synthesized bysimple hydrothermal method with diatomite as carrier. It was found that under the action of hydrogenperoxide (H2O2), the imidacloprid (IMD) could be effectively degraded by the composite. Systemic evaluationswas conducted on effects of pH value, catalyst dosage, H2O2 concentration, initial IMD concentration,and anion on how effeciently this new photocatalytic composite can degrade IMD. The results showthat the p-n heterojunction formed between the two contact surfaces of Ag2O nanoparticle and BiVO4promotes the charge transfer between the interfaces, inhibits recombination of electrons and holes,and thus significantly improves the catalytic performance. The stability and reusability of Ag2O/BiVO4/diatomite composites were tested with ICP experiment and cyclic experiment. Active substancecapture experiment and ESR reveal the photocatalytic reaction mechanism and confirm that superoxideradical (O2), hydroxyl radical (OH), and h+ can promote the degradation of IMD. The degradation productsand the intermediates of IMD were identified through LC-MS, on a basis of which the possible transformationpathway was proposed. In situ synthesis of p-type Ag2O and n-type BiVO4 heterojunctioncomposites has opened a new direction for photocatalytic degradation of organic pollutants.

Study on preparation of inorganic binder stabilized material with large dosage of phosphogypsum

Sun Qiqi,Tao Liangjing,Li Xin,Xu Wei,Yao Shuo,Li Jinpeng,Ren Qi-Fang,Chen Yue’e,Xu Chunshan,Wu Zilong,Zhu Yuelei,Ding Yi,오원춘 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.6

With the rapid development of the phosphorus chemical industry, a large amount of phosphogypsum (PG) is discharged. The impurities in PG pollute the surrounding soil, the groundwater and the air in the long-term storage. In this paper, PG as the main raw material is used to study the stable material of inorganic binder in the condition of large dosage of PG. With PG and graded gravel as stabilized materials, and cement and fly ash as binder, materials are mixed to determine the influence of PG ratio and the amount of activator on the mixture. The results show that the mechanical strength of the mixture increased first and then decreased with the increase in the proportion of PG replacing graded gravel in the mixture. The activator significantly improves the properties of the material system and makes a significant contribution to the curing of PG. The best ratio of pretreated PG: cement: fly ash: aggregate is 50:6:5:39. The amount of activator added by the external mixing method is 0.3%, which can make the unconfined compressive strength reach 4.1 MPa after 7d. Finally, water-soluble phosphorus leaching experiment is conducted for all the samples. The results show that phosphorus leaching is so little that no secondary pollution of the environment is caused.

NADH-dependent butanol dehydrogenase from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis

Xue Bai,Jing Lan,Shanru He,Tingting Bu,Jie Zhang,Lulu Wang,Chunshan Quan,Ki Hyun Nam,Nam-Chul Ha,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.2

Fusobacterium nucleatum is a dangerous pathogen, and it has been linked to a variety of health problems, including cancer, periodontal disease, and pregnancy complications. F. nucleatum butanol dehydrogenase (FnYqdH) is a group of dehydrogenase enzymes that facilitate interconversion between butyraldehyde and butanol at the expenditure of a cofactor NAD (P)H. In this study, FnYqdH was successfully expressed and purified using Ni-NTA affinity, Q anionexchange, and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.0 Å. The crystal belonged to the orthorhombic space group of I222, with unit-cell parameters of a = 64.77, b = 78.85, and c = 215.22 Å. The Matthews coefficient and solvent content were estimated to be 3.19 Å3 Da–1 and 61.49%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule. Size-exclusion chromatography suggested that FnYqdH prefers to exist as a dimer in the solution.

Pyridoxal phosphate homeostasis protein FnYggS from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis

Shanru He,Chenyuan Yuan,Xue Bai,Tingting Bu,Jie Zhang,Lulu Wang,Chunshan Quan,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.2

Anaerobic gram-negative bacterium Fusobacterium nucleatum (F. nucleatum) has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer. In F. nucleatum, YggS (FnYggS) is an important part of bacterial cell walls and belongs to the COG0325 gene family. In this study, YggS from FnYggS was successfully expressed and purified using Ni-NTA affinity and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.08 Å. The preliminary crystallographic analysis suggested that FnYggS crystal belongs to the monoclinic space group P21 with a = 37.93 Å, b = 146.38 Å, and c = 74.13 Å, α = γ = 90.00° and β = 93.36°. The asymmetric unit contained approximately three monomer of FnYggS, giving a crystal volume per mass (VM) of 2.64 Å3 Da–1 and a solvent content of 53.50%.

Clostridium beijerinckii glyceraldehyde-3-phosphate dehydrogenase GAPDH: purification, crystallization, and X-ray crystallographic analysis

Jie Zhang,Yuanyuan Chen,Tingting Bu,Xue Bai,Shanru He,Lulu Wang,Chunshan Quan,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.3

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly abundant glycolytic enzyme. It plays a pivotal role for the energy and carbon metabolism of most organisms including industrial bacteria. It catalyzes the two step oxidative phosphorylation of D-glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate using inorganic phosphate and nicotinamide adenine dinucleotide (NAD+) as cofactor. In this study, GAPDH from C. beijerinckii (CbGAPDH) was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 1.60 Å. The crystal belonged to the hexagonal space group P6222, with unit-cell parameters of a = 120.6, b = 120.6, and c = 122.1 Å. The Matthews coefficient and solvent content were estimated to be 3.50 Å3 Da–1 and 64.90%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule.