Determination of Parameters for Expolinear Growth Equation of Single-node Cutting Roses in a Plant Factory

Yong-Beom Lee,Kyung-Hwan Yeo,Yong Yeol Cho 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2

A strategy using MSH6, PMS2 immunohistochemistry to identify Lynch syndrome in patients with endometrial cancer (SPICH)

( Yeo Rae Kim ),( Dong Hoon Suh ),( Jae Hong No ),( Yong Beom Kim ),( Ki Dong Kim ) 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-

Antireflective silicon nanostructures with hydrophobicity by metal-assisted chemical etching for solar cell applications

Yeo, ChanIl,Kim, Joon Beom,Song, Young Min,Lee, Yong Tak Springer 2013 Nanoscale research letters Vol.8 No.1

<P>We present broadband antireflective silicon (Si) nanostructures with hydrophobicity using a spin-coated Ag ink and by subsequent metal-assisted chemical etching (MaCE). Improved understanding of MaCE, by conducting parametric studies on optical properties, reveals a design guideline to achieve considerably low solar-weighted reflectance (SWR) in the desired wavelength ranges. The resulting Si nanostructures show extremely low SWR (1.96%) and angle-dependent SWR (<4.0% in the range of 0° to 60°) compared to that of bulk Si (SWR, 35.91%; angle-dependent SWR, 37.11%) in the wavelength range of 300 to 1,100 nm. Relatively large contact angle (approximately 102°) provides a self-cleaning capability on the solar cell surface.</P>

Biosynthesis of Nonimmunosuppressive FK506 Analogues with Antifungal Activity

Beom, Ji Yoon,Jung, Jin A,Lee, Kyung-Tae,Hwangbo, Areum,Song, Myoung Chong,Lee, Yeonseon,Lee, Soo Jung,Oh, Ji Hoon,Ha, Sang-Jun,Nam, Sang-Jip,Cheong, Eunji,Bahn, Yong-Sun,Yoon, Yeo Joon American Chemical Society and American Society of 2019 Journal of natural products Vol.82 No.8

<P>A reduction in the strong immunosuppressive activity of FK506 (<B>1</B>) is essential for developing this compound as an antifungal agent. Seven new FK506 analogues modified at both the FK506-binding protein 12- and the calcineurin-binding regions were biosynthesized. 9-DeoxoFK520 (<B>7</B>) exhibited a >900-fold reduction in the <I>in vitro</I> immunosuppressive activity but maintained significant antifungal activity, indicating that the C-9 and C-21 positions are critical for separation of immunosuppressive and antifungal activities. <B>7</B> exhibited robust synergistic antifungal activity with fluconazole. FK506 (<B>1</B>) is a 23-membered macrolide produced by several <I>Streptomyces</I> species and is used as an immunosuppressive drug to prevent the rejection of transplanted organs. FK506 has also exhibited antifungal, neuroprotective, and neuroregenerative activities. In humans, FK506 binds to FK506-binding protein (FKBP) 12, and the resulting FKBP12-FK506 complex interacts with a Ca<SUP>2+</SUP>-calmodulin-dependent phosphatase, calcineurin (CaN). Inactivation of CaN by forming the FKBP12-FK506-CaN ternary complex prevents the activation of nuclear factor of activated T cells (NF-AT), inhibiting the production of interleukin-2 and subsequent T-cell proliferation. This CaN signaling pathway also plays a critical role in the growth and pathogenesis of major fungal pathogens such as <I>Cryptococcus neoformans</I>, <I>Candida albicans</I>, and <I>Aspergillus fumigatus</I>. Therefore, the synthesis of FK506 analogues that can discriminate human FKBP12/CaN from its fungal counterparts may separate antifungal activity from the immunosuppressive activity, thereby allowing the development of a novel antifungal agent.</P> [FIG OMISSION]</BR>

알츠하이머질병 모델동물인 Tg2576 마우스를 이용한 미나리 알코올추출물의 기억력 개선 효능

원범영(Beom Young Won),신기영(Ki Young Shin),하현지(Hyun Jee Ha),장근아(Keun-A Chang),윤여상(Yeo Sang Yun),김예리(Ye Ri Kim),박용진(Yong Jin Park),이형근(Hyung Gun Lee) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.6

본 연구는 천연 식물이 기억력 개선에 미치는 영향을 검토하기 위하여 총 7가지 식물에 대하여 아세틸콜린분해효소 활성 억제력을 측정하였다. 특히 미나리 알코올추출물(18.76%)의 억제력이 가장 우수하였으며, 미나리 알코올추출물에 대한 추가 연구를 수행하였다. Tg2576 마우스의 기억력에 미치는 영향을 검토하기 위하여 미나리 알코올추출물 50 mg/kg으로 3개월간 경구투여 후 수동회피테스트로 인지기능변화를 측정하였고, 뇌 속의 아세틸콜린분해효소 활성 억제, 베타아밀로이드1-42 단백질 생성 억제력을 측정하였다. 그 결과, 수동회피 테스트에서 미나리추출물을 투여한 Tg 마우스군은 181.77초로 생리식염수를 투여한 Tg 마우스군과 비교하여 머무름 시간이 유의적으로 증가하게 나타났다. 베타아밀로이드1-42 단백질 농도 측정 시 미나리 알코올추출물에 의하여 축적 농도가 985.19 pmol/g으로 감소하였으며, 생리식염수를 투여한 Tg 마우스군과 유의적 차이가 있었다. 추가적인 효소억제력 실험 결과, 미나리 알코올추출물의 아세틸콜린분해효소 활성억제에 대한 50% 활성억제농도(IC<SUB>50</SUB>)값은 991.77 μg/mL로 나타났으며, Lineweaver-Burk Plot 결과, 무경쟁적 저해로 나타났다. 따라서 미나리 알코올추출물은 Tg2576 형질전환 마우스의 인지기능을 개선시키며, 콜린성 신경시스템을 보호하는 물질로 판단된다. 미나리 알코올추출물은 기억 및 학습 증진에 효과적으로 작용하는 천연식물로써 이상의 결과를 근거로 한 미나리 소재의 다양한 기능성제품 개발과 부가가치 향상이 가능할 것으로 판단된다. This study was conducted to investigate the effect of herbs on memory improvement by focusing on their cholinergic functions in Tg2576 mice. Seven herbs were used to obtain extracts by using alcohol and water. In screening test for cholinergic activities of the extracts, acetylcholinesterase (AChE) activity was highly inhibited in Oenanthe javanica alcohol extract (OJAE, 18.76%) as compared with the others. The OJAE-treated Tg2576 (Tg-OJAE) groups showed the statistically significant increases of latency time in passive avoidance test. Also, it was found that the concentration of Aβ1-42 was significantly reduced in Tg-OJAE groups compared to non-treated Tg2576 groups. In the additional enzyme test, it was found that IC<SUB>50</SUB> of OJAE was 991.77 μg/mL and OJAE acted as an uncompetitive inhibitor of AChE. Therefore, it seemed that OJAE can be used for the development of processed foods for memory improvement.

딜의 水耕栽培에 適合한 培養液 開發

여경환(Yeo Kyung-Hwan),이용범(Lee Yong-Beom) (사)한국생물환경조절학회 1997 시설원예‧식물공장 Vol.6 No.4

Effect of ethanol and glycerol on suppression rates of various crop pathogens

Kim, Yeo Hyeon,Jang, Se Ji,Yun, Young Beom,Kim, Sang Soo,Kuk, Yong In Gaurav Publications 2017 Research on Crops Vol.18 No.1

미나리 추출물의 기억력 손상 억제와 산화스트레스 억제 효과 및 Isorhamnetin 분석

원범영(Beom Young Won),신기영(Ki Young Shin),하현지(Hyun Jee Ha),위지향(Ji-Hyang Wee),윤여상(Yeo Sang Yun),김예리(Ye Ri Kim),박용진(Yong Jin Park),정경옥(Kyoung Ok Jung),성혜미(Hea Mi Sung),이형근(Hyung Gun Lee) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.1

본 연구는 한국인이 일상생활에서 주로 상용하는 미나리에 대해 기억력 관련 효과와 항산화 효과, 미나리 추출물의 flavonoid 중 isorhamnetin을 분석하여 기능성식품 개발 및 이용에 기여하고자 실시하였다. Acetylcholinesterase의 활성 억제를 측정한 결과 화순미나리 추출물이 28.59%로 가장 높게 나타났으며, 나주미나리 추출물 25.11%와 유의적인 차이가 없었다. SH-SY5Y 세포사멸에 미치는 영향을 측정한 결과 DMSO와 비교하여 화순미나리 추출물 37.23%와 나주미나리 추출물 36.68%로 세포사멸이 유의적으로 감소하였다. 수동회피실험 결과 scopolamine 처리에 의해 기억력이 손상된 동물모델에서 미나리 추출물을 투여하였을 때 vehicle control보다 latency time이 높게 나타났다. DPPH 및 ABTS 라디칼 소거능 측정 결과 화순, 나주, 의령 미나리 추출물 모두 미나리 처리 그룹군 간에도 유의적 차이가 있었고(P<0.05), 화순미나리 추출물이 총 폴리페놀 함량 117 mg/g, 총 플라보노이드 함량 30 mg GAE/g으로 가장 높게 측정되었다. TBARS 측정 결과 미나리 추출물 440μg/mL의 농도에서 화순미나리 추출물이 50.7%의 억제력으로 가장 높았으며, 나주와 의령미나리 추출물보다 유의하게 높았다. 미나리 추출물의 플라보노이드 중 isorhamnetin을 분석한 결과 표준용액과 미나리 추출물의 머무름 시간이 14분대로 피크 유지시간이 일치하였으며, 동일한 spectrum을 나타내어 정확한 isorhamnetin의 분리를 확인하였다. 또한 LC/MS/MS를 통한 분석 결과 표준용액과 미나리 추출물에서 동일 시간대에 TIC를 확인하였고 precursor ion은 317 [M-H]+ m/z, product ion은 302 [M-H]+ m/z로 확인할 수 있었다. 이러한 연구 결과에 기초하여 미나리추출물은 천연 항산화제와 기억력 개선제로써의 활용 가치를 시사한다고 사료된다. 또한 건강기능성 소재로서 식품산업 분야의 이용률 향상에 도움이 될 것으로 판단된다. This study was conducted to investigate effect of the acetylcholinesterase inhibitor activity, the protective effect of the extract on SH-SY5Y cell death by H₂O₂, the memory improvement from scopolamine-induced rat. Moreover, the antioxidant activity of isorhamnetin from the dropwort (Oenanthe javanica) was investigated. Acetylcholinesterase inhibitor activity was highest (28.59%) in Hwasun O. javanica extract (H-OJE). H-OJE and Naju O. javanica extract (N-OJE) were not significantly different. SH-SY5Y cell death deceased to 37.23% and 36.68% for H-OJE and N-OJE, respectively, following treatment with the extracts. O. javanica extracts showed a protective effect against H₂O₂-induced neurotoxicity. Treatment with O. javanica extracts slightly improved scopolamine-induced (1 mg/kg, i.p.) memory impairment in rats. H-OJE contained the highest total phenolic and flavonoid contents of 117 mg/g and 30 mg gallic acid equivalents/g, respectively, and had a DPPH radical scavenging activity (SC50) of 113.8 μg/mL and ABTS radical scavenging activity of 48.2 μg/mL, which was higher than the other extracts. The thiobarbituric acid reactive substances value was highest (50.2%) in H-OJE. Antioxidant activity differed significantly among dropwort extracts. Isorhamnetin was known as one of the flavonoid and for having neuroprotective effect. So we analyzed acid-hydrolyzed O. javanica extract HPLC. The results were that peak at 14 min and spectrum of the extracts was consistent with standard solution. The results of LC/MS/MS analysis were that the extract and standard solution were confirmed total ion chromatogram at identical time, precursor ion was 317 [M-H]+ m/z, product ion was 302 [M-H]+ m/z. Overall, the results showed that the dropwort extract led to memory improvement and had antioxidant activity. Based on these finding, further research to investigate the production of ethanol extract of dropwort as a processed food is warranted.

당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.

그레이브스병 환자에서 방사선옥소 치료가 혈청 가용성 Interleukin-2 Receptor (IL-2R)에 미치는 영향

여준기 ( Yeo Jun Gi ),박정모 ( Park Jeong Mo ),조성래 ( Jo Seong Lae ),남복현 ( Nam Bog Hyeon ),한승범 ( Han Seung Beom ),박근용 ( Park Geun Yong ),이인규 ( Lee In Gyu ),안성훈 ( An Seong Hun ),이재태 ( Lee Jae Tae ),이규보 ( Lee 대한내과학회 1993 대한내과학회지 Vol.44 No.5

연구배경 : 그레이브스병은 각종 자가 항체들의 존재 및 면역조절에 관여하는 T임파구 아형의 수적변화 또는 기능장애가 동반되는 것으로 알려져 있다. 치료받지 않은 갑상선 기능항진 상태의 그레이브스병에서 활성화된 T임파구와 활성화된 T임파구에서 유리되는 혈청 가용성 IL-2R는 정상인보다 증가되어 있으며, 방사선옥소 또는 항갑상선제제로 치료함에 따라 감소된다고 한다. 따라서 혈청 가용성 IL-2R는 T임파구의 활성화 및 그레이브스병의 활동도를 반영하는 면역학적 지표로 이용할 수 있다. 방법 : 저자들은 갑상선 기능항진상태의 그레이브스병환자 35명을 대상으로 방사선옥소 또는 항갑상선제제와 방사선 옥소를 병용 투여하여 이들의 치료전, 후의 혈청 가용성 IL-2R 측정하여 비교하였다. 혈청 가용성 IL-2R는 2 noncompeting monoclonal antibody를 이용하는 sandwich enzyme immunoassay법으로 측정하였다. 결과 : 혈청 가용성 IL-2R치는 전체 대상환자에서 치료전 1169.0±691.6U/ml, 치료 1개월후 945.7±86.5U/ml, 치료 3개월후 600.2±362.0U/ml로 치료전과 치료후 1개월, 치료전과 치료후 3개월, 치료후 1개월과 치료후 3개월치를 비교하여 각각 통계학적으로 유의한 감소를 보였다(p<0.05). 혈청 T3 및 가용성 IL-2R치는 치료전(r=0.4, p<0.05), 치료후 1개월(r=0.7, p<0.01), 치료후 3개월치(r=0.7, p<0.01)에서 각각 통계학적으로 유의한 상관관계를 나타내었다. 또한 혈청 T4 및 가용성 IL-2R치도 치료전(r=0.4, p<0.05), 치료후 1개월(r=0.4, p<0.05), 치료후 3개월(r=0.8, p<0.01)에서 각각 통계학적으로 유의한 상관관계를 나타내었다. 결론 : 이상의 결과로서 혈청 가용성 IL-2R치의 측정은 그레이브스병의 활동도를 판정하는데 좋은 지표가 된다고 하겠다. Backgrounds: Graves` disease is associated with the presence of autoantibodies, alterations of T cell subsets, and abnormalities of T cell function. Activated T cells and the level of serum soluble IL-2R, which was released from activated T cells, were increased in untreated hyperthyroid Graves` disease compared to those in normal individuals and decreased with radioiodine and/or antithyroid drug treatment. Thus, soluble IL-2R can be utilized as immunological marker for activity of Graves` disease and activation of T cells. Methods: We studied 35 patients with hyperthyroid Graves` disease and administered radioiodine and/or antithyroid drugs. We measured serum soluble IL-2R by enzyme-linked immunosorbent assay, before and after treatment, respectively. Results: Serum soluble IL-2R levels showed statistically significant decrement in accordance with the decline of thyroid hormone levels during radioiodine treatment. There were statistically significant correlation between slouble IL-2R released from activated T cells is a useful immunological marker of disease activity in hyperthyroid Graves` disease. Conclusions: Thus, in vivo measurement of serum soluble IL-2R released from activated T cells is a useful immunological marker of disease activity in hyperthyroid Graves` disease.