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Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.
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Yan-Ni Wu,Hai-Fu Guo,Peng Hu,Xiao-Peng Xiao,Zhao-Wang Xiao,Shi-Jun Liao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.7
Three types of ternary low-platinum nanocatalysts, alloy PdPtIr/C, core–shell PdPt@PtIr/C and Pd@PtIr/C, have been prepared, and their catalytic behaviors toward methanol oxidation reaction (MOR)/oxygen reduction reaction (ORR) are comparatively investigated via cyclic voltammetry and chronoamperometry analysis in an acidic medium. Through a two-step colloidal technique, the synthesized core–shell structured catalyst PtPd@PtIr/C with alloy core and alloy shell show the best catalytic activity toward MOR and the best poisoning tolerance. The alloy PdPtIr/C catalyst prepared via a one-step colloidal technique exhibits the best performance toward ORR among the three catalysts. All the three catalysts are characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and other characterization techniques.
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Peng Chen,Lei Yan,Shuang Zhang,Zhengrong Wu,Suyue Li,Xiaojuan Yan,Ningbo Wang,Ning Liang,Hong Yu Li 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1
A stirring bioreactor packed with a carbon fiber textiles (FT) biofilm formed by Bacillus subtilis was used to produce vanillin from ferulic acid. Biofilm formation was characterized by scanning electron microscopy. The interactive effects of three variables on vanillin molar yield (M) and conversion efficiency of ferulic acid (E) were evaluated by response surface methodology (RSM) with a Box-Behnken design (BBD). The optimal conversion conditions with a maximum overall desirability D of 0.983 were obtained by a desirability function. Considering the actual operation, the confirmation tests were performed using the slightly modified optimal conditions (initial ferulic acid concentration 1.55 g/ L, temperature 35oC, stirring speed 220 rpm). The results showed that M and E were 57.42 and 93.53%, respectively. This was only 1.03% and 1.87%, respectively, different from the predicted values, confirming the validity of the predicted models. These revealed that the stirred packed reactor could be successfully used in vanillin bioconversion from ferulic acid.
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Kinetostatic Modeling of Bridge-Type Amplifiers Based on Timoshenko Beam Constraint Model
Peng-Bo Liu,Peng Yan 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.9
In this paper, we develop an accurate kinetostatic modeling method for bridge-type amplifiers by incorporating the impacts of nonlinear shear effect, center-shifting and load-stiffening. To account for the nonlinear stress-stiffening and shear effects, a Timoshenko Beam Constraint Model (TBCM) is first investigated to accurately predict the deformations of the short beam flexure hinges popularly employed in the bridge-type amplifiers. Accordingly, a flexible branched chain model is established for bridge-type amplifiers, where the input and output displacements, as well as the displacement amplification ratio and the input stiffness, are derived. The results indicate significant nonlinearities of the amplification ratio and the input stiffness with respect to the driving forces, which are further verified by the finite element analysis (FEA) results. The proposed modeling method can derive a more accurate model of the bridgetype amplification mechanism to capture its kinetostatic behaviors, which can better support the design and control of flexure mechanism based nano-manipulating systems.
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HOW CHINESE FIRMS RESPONSE TO FOOD NUTRITION LABEL REGULATION
Peng Zou,Yan Liu 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7
Recent decades have seen a number of policies have been launched to promote information disclosure on food products marketed in developed countries. There has also been increasing efforts to promote healthy eating in developing economies (Cowburn & Stockley 2005). A good example appears in China, where, in an effort to improve health education, nutrition labels became mandatory under the Nutrition Labeling Act (NLA) since 2013. Additionally, food labeling regulations were issued by India, Mercosur members and South Africa. While nutritional labeling has received a lot of attention both in academia and from the press, there are some key gaps in the nutrition labeling literature. One issue of how standardized information requirements affect firm marketing responses (e.g., advertising levels. R&D investment, and corporate social responsibility programs) and performance has been understudied. Another clear gap in the literature is how nutritional labeling requirements affect developing economies.
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Mammary Cell Turnover under High Temperature during the Dry Period in Dairy Cows
Peng, Xiaoqing,Lu, Lin,Li, Yan,Yan, Peishi Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4
The influence of high temperature on mammary cell turnover during the dry period is still unclear. The objective of this study was to investigate mammary cell turnover and p53 protein expression in the mammary tissue under high temperature conditions. Mammary gland biopsy samples from 8 dairy cows were obtained at 7, 25, 40, and 53 d during the dry period in summer or spring (n = 4, each season). Cell cycle, cell turnover, and p53 protein expression were analyzed by flow cytometry. During the dry period in summer, the percentage of mammary epithelial cells in the G0/G1 phase was the highest, but those in the S and G2/M phases were lower. However, the proportion of cells in the different stages of the cell cycle was not significantly different among the different biopsy time points, except in the G2/M phase. Under different temperature conditions, the cells were significantly different in their apoptotic rate and proliferation index; moreover, the tendencies of these indicators to change significantly differed. In general, the samples under high temperature conditions showed significantly lower apoptotic rates and proliferation indices. Under high temperature conditions, the apoptotic rate and proliferation index were the lowest (2.17% and 3.26%, respectively) at day 40, and the highest at day 53 (3.67% and 4.61%, respectively). However, under normal temperature conditions, the values of these indicators were the lowest (7.60% and 5.54%, respectively) at day 7, and almost the highest at day 25 (12.85% and 6.47%, respectively). Moreover, p53 protein expression was significantly higher under high temperature conditions than under normal temperature conditions, except at day 25. The level of p53 protein was the lowest (13.10%) under high temperature conditions at day 25, but was the highest (26.07%) under normal temperature conditions. Our findings suggest that high temperature delayed the G2/M phase of the cell cycle and the cell turnover rate, but remarkably increased p53 protein expression. Thus, the results indicate that high temperature extends the recovery period of mammary epithelial cells.
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Flexure-Hinges Guided Nano-Stage for Precision Manipulations: Design, Modeling and Control
Peng-Bo Liu,Peng Yan,Zhen Zhang,Tong-Tong Leng 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
In this paper, a novel piezoelectric actuator driven nano-stage with bridge type mechanism is studied from the perspectives of design optimization, dynamical modeling, as well as controller synthesis for high precision manipulation purposes. FEM (Finite Element Method) analysis and dynamical modeling are provided to derive the system model including the hysteresis nonlinearity. Considering the complexities of dynamical uncertainties and hysteresis nonlinearity, an active disturbance rejection controller is developed consisting of extended state observer (ESO), state feedback controller and profile generator. With the proposed algorithm, the nonlinear dynamics, system uncertainties and external disturbances can be treated as part of the “total disturbances”, such that the extended state observer can be used to estimate and suppress the effects of these complex dynamics. The proposed control algorithm is deployed in real time implementations on the designed nano-stage, where experimental results demonstrate good control performance in terms of high precision positioning, hysteresis compensation and disturbance rejection.
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Inhibition of Bone Resorption by Econazole in Rat Osteoclast-like Cells through Suppressing TRPV5
Peng Yan,Tang Li,Meng Bo,Liu Die,Liang Xing 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6
Osteoclasts are primary bone resorption cells and intervention in osteoclast activation is considered an effective therapeutic approach to treatment of bone diseases involving osteoclasts. TRPV5 was detected in osteoclasts and it has been thought to take part in the transportation of the degraded calcium in the resorption lacuna, which is essential for bone resorption. The aim of the present study was to examine the effects of a modulator of calcium dynamics, econazole, on the expression of TRPV5 and bone resorption activity in rat osteoclast-like cells (OLCs). OLCs were obtained by co-culturing rat bone marrow cells with osteoblasts and then culturing with different concentrations of econazole (0.01, 0.1, 1.0, 10.0 μmol/L). Cell counting and staining protocols were used to determine whether econazole influenced the survival of OLCs. Expression of TRPV5 in response to econazole treatment was assessed by western blotting. Bone resorption activity of OLCs was determined by measuring the resorption area of dentin slices with a microscope and a digital image analysis system. Additionally, Ca^2+ inside OLCs was tested. We found that econazole inhibited expression of TRPV5 in a dose dependent manner while it had no influence on the survival of OLCs and it therefore inhibited bone resorption activity in rat OLCs. Ca^2+ inside OLCs increased, suggesting a limited compensatory mechanism to make up for inhibition of TRPV5 effects.
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