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Wang, Xue-Mei,Cui, Jiu-Wei,Li, Wei,Cai, Lu,Song, Wei,Wang, Guan-Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
The COPS3 gene has stimulating effect on cell proliferation and progression of osteosarcomas and related cells. However, the features of COPS3 and its potential application as a therapeutic target in other cancers has not yet been studied. In this study, therefore, the effect of COPS3 silencing via COPS3 siRNA on lung cancer cell proliferation was examined. Expression levels of COPS3 gene in COPS3 siRNA infected cells and control siRNA infected cells were compared with real time PCR and Western blot analysis. Cell proliferation levels were comprehensively analyzed by MTT, BrdU incorporationy, and colony formation assays. For mechanistic assessment the effects of COPS3 silencing on cell cycle and apoptosis were analyzed using flow cytometry. Results showed that successful silencing of the COPS3 gene at both translational and transcriptional levels significantly reduced the proliferation and colony formation by lung cancer cells (p<0.01). Flow cytometry showed cell cycle arrest in the G0/G1 phase after COPS3 silencing, and more importantly, apoptosis was induced as a result of COPS3 knockdown, which negatively affected cell survival. Therefore, these results provide another piece of important evidence that the COPS3 gene expressed in lung cancer cells may play a critical role in stimulating proliferation. Down-regulation of COPS3 could significantly inhibit lung cancer cell growth, which was most likely mediated via induction of cell cycle arrest in G0/G1 phase and apoptosis.
Xue-Wei Wang,Shen-Ao Zhang,Cui Guo,Zhi-hao Yuan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.4
In this paper, it is described that the motion of water droplets on the hydrophobic surface of ZnO nanorod array impregnated lubricant, is called slippery liquid-infused porous surface (SLIPS). The energy gradient required to induce the motion of the droplets is created on the boundary of superhydrophobic ZnO nanorod array and SLIPS. Because of the lower viscous force of SLIPS, the water droplet can rapidly move for longer distance on the surface. In view of changing the release distance of water droplet, the mechanism for the rapid movement is discussed. The results indicate that the movement distance of water droplet markedly increases with the increasing of the release distance. Because the potential energy of the height is converted into kinetic energy, the water droplet intensively collides the interface between ZnO nanorod array and SLIPS. This impact makes the water droplet distort, which enhances the driving force. These new findings will not only deepen our understanding of the relationship between surface structure and dynamic wetting properties, but also afford the new notion and beneficial reference for designing liquid droplet transportation devices in micro-fluidic systems.
( Wei Wang ),( Hua Sheng Wang ),( Hong Bo Hu ),( Hua Song Peng ),( Xue Hong Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5
As a global regulatory gene in Streptomyces, afsR can activate the biosynthesis of many secondary metabolites. The effect of afsR on the biosynthesis of a phenazine metabolite, lomofungin, was studied in Streptomyces lomondensis S015. There was a 2.5-fold increase of lomofungin production in the afsR-overexpressing strain of S. lomondensis S015 N1 compared with the wild-type strain. Meanwhile, the transcription levels of afsR and two important genes involved in the biosynthesis of lomofungin (i.e., phzC and phzE) were significantly upregulated in S. lomondensis S015 N1. The optimization of metal chlorides was investigated to further increase the production of lomofungin in the afsR-overexpressing strain. The addition of different metal chlorides to S. lomondensis S015 N1 cultivations showed that CaCl2, FeCl2, and MnCl2 led to an increase in lomofungin biosynthesis. The optimum concentrations of these metal chlorides were obtained using response surface methodology. CaCl2 (0.04 mM), FeCl2 (0.33 mM), and MnCl2 (0.38 mM) gave a maximum lomofungin production titer of 318.0 ± 10.7 mg/l, which was a 4.1-fold increase compared with that of S. lomondensis S015 N1 without the addition of a metal chloride. This work demonstrates that the biosynthesis of phenazine metabolites can be induced by afsR. The results also indicate that metal chlorides addition might be a simple and useful strategy for improving the production of other phenazine metabolites in Streptomyces.
NEW FORM OF INDUSTRIAL FREQUENCY WATTMETER BASED ON DOUBLE THREE SLOPE PRINCIPLE
Wei, Wang Xue,Sheng, Fei Zheng,Chen, Liu Bei,Lin, Wang 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
This paper proposes a double three slope multiplying A/D converter and introduces the new A/D converter application with sample/hold technique and microprocessor in the field of industrial power measurement. The new A/D converter has low price. The wattmeter based on the new A/D converter and microprocessor now is used, its precision is better than 0.1%.
Wei Bin,Wang Ya-Kun,Yu Jin-Biao,Wang Si-Jia,Yu Yan-Lei,Xu Xue-Wei,Wang Hong 한국미생물학회 2021 The journal of microbiology Vol.59 No.10
C-Glycosides are an important type of natural product with significant bioactivities, and the C-glycosidic bonds of C-glycosides can be cleaved by several intestinal bacteria, as exemplified by the human faeces-derived puerarin-degrading bacterium Dorea strain PUE. However, glycoside hydrolases in these bacteria, which may be involved in the C-glycosidic bond cleavage of C-glycosides, remain largely unknown. In this study, the genomes of the closest phylogenetic neighbours of five puerarin-degrading intestinal bacteria (including Dorea strain PUE) were retrieved, and the protein-coding genes in the genomes were subjected to sequence similarity network (SSN) analysis. Only four clusters of genes were annotated as glycoside hydrolases and observed in the genome of D. longicatena DSM 13814T (the closest phylogenetic neighbour of Dorea strain PUE); therefore, genes from D. longicatena DSM 13814T belonging to these clusters were selected to overexpress recombinant proteins (CG1, CG2, CG3, and CG4) in Escherichia coli BL21(DE3). In vitro assays indicated that CG4 efficiently cleaved the O-glycosidic bond of daidzin and showed moderate β-D-glucosidase and β-D-xylosidase activity. CG2 showed weak activity in hydrolyzing daidzin and pNP- β-D-fucopyranoside, while CG3 was identified as a highly selective and efficient α-glycosidase. Interestingly, CG3 and CG4 could be selectively inhibited by daidzein, explaining their different performance in kinetic studies. Molecular docking studies predicted the molecular determinants of CG2, CG3, and CG4 in substrate selectivity and inhibition propensity. The present study identified three novel and distinctive glycoside hydrolases, highlighting the potential of SSN in the discovery of novel enzymes from genomic data.
Abnormal resistanceetemperature characteristic of the melting Bi nanowires
Xue-wei Wang,Chao Ma,Bing-cheng Fang,Zhi-hao Yuan 한국물리학회 2014 Current Applied Physics Vol.14 No.11
There are no reports about the electronic transport behavior of the melting metal nanostructures because the morphology of nanostructures cannot be kept under the melting condition. Here, the electronic properties of the melting Bi nanowires are investigated using the pore confinement of anodic aluminum oxide template. The results indicate that with the increase of temperature the resistance of Bi nanowires has a transition from the positive temperature coefficient of resistance before fusion to the negative one after fusion. Moreover, as the temperature gradually increases, the resistance of the melting Bi nanowires rapidly decreases at first, and then tardily decreases. This research provides fundamental and valuable information for exploring and designing the new electronic devices under the high temperature.
Wei Xue,Hang Wang,Zuodi Pei,Wei Zhao,Yong-Sheng Tian,Ri-He Peng,Quan-Hong Yao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it difficult to obtain a large number of transformants necessary to thoroughly cover a large library. We report here that pre-coating DNA with polyethyleneimine and dextran sulfate increased gene integration significantly in Pichia electroporation. This improvement on integrative efficiency has been proved in different voltage, resistance, cell phase, and DNA concentrations. The condensed DNA nanoparticles make Pichia available as a host for screening large libraries of random mutants.
Wei Xue,Zhang Jixing,Wang Nan,Sun Mengdi,Ding Xue,Xu Hui,Yu Xiumin,Yue Wenran,Huo Hongyan,Yu Lili,Wang Xiaoyu 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.3
REVEILLE (RVEs) transcription factors play an important role in regulating circadian clock, plant growth and development, and stress responses. While RVEs have been identifi ed in various plant species, comprehensive genome exploration and analysis of RVEs in castor ( Ricinus communis L.) have not been previously reported. In this study, we identifi ed RcRVEs in the castor genome through sequence alignment and conducted an analysis encompassing gene structures, protein motifs, physicochemical properties, transcription patterns under cold stress, diff erent tissues, and diurnal rhythm. A total of fi ve RcRVEs were pinpointed in the castor genome. Evolutionary tree analysis categorized these RcRVEs into three distinct groups, implying that diff erent RcRVEs may fulfi ll distinct biological functions. Furthermore, several abiotic stresses and hormone-responsive cis -acting elements were found in the promoters of the RcRVEs . RNA-sequencing (RNA-seq) analysis revealed that RcRVE1 and RcRVE7 were induced by cold stress and exhibited upregulation over time, indicating their involvement in regulating cold adaptation in castor plants. Five RcRVEs have notably higher expression levels in leaves compared to other tissues. The expression of all RcRVEs displayed diurnal rhythms under light/dark cycles. This study provides valuable insights into the potential functions of RcRVEs in regulating castor’s growth and development, and responses to cold stress.