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( Xinyue Chang ),( Chengli Teng ),( Haizhen Wu ),( Jiang Ye ),( Qiyao Wang ),( Huizhan Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8
Edwardsiella piscicida is the causative agent of edwardsiellosis, which has caused enormous economic losses worldwide. In our previous research, an attenuated live vaccine known as WED and based on the virulent strain E. piscicida EIB202 can effectively protect turbots against edwardsiellosis via intraperitoneal injection, while vaccination by immersion exhibits a weaker effect. During the development of the immersion vaccine, we surprisingly found the counts of ΔpEIB202/ EIB202 colonized on zebrafish were 100 times lower than those of EIB202. However, pEIB202 carries 53 predicted ORFs and has several copies in E. piscicida EIB202, impeding the study of its function. Thus, the replication region is located at a 1,980 bp fragment (from 18,837 to 20,816 bp), containing a transcriptional repressor and a replication protein. Moreover, the minimal replication plasmid, named pRep-q77, has low copies in both E. coli and E. piscicida, but is more stable in E. piscicida than in E. coli. This work lays a foundation for further examination of the function of the virulence plasmid pEIB202.
Min Li,Lujun Song,Xiaodong Gao,Wenju Chang,Xinyu Qin 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.4
Type 1 diabetes mellitus is caused by the autoimmune destruction of β cells within the islets. In recent years,innate immunity has been proposed to play a key role in this process. High-mobility group box 1 (HMGB1), an inflammatory trigger in a number of autoimmune diseases,activates proinflammatory responses following its release from necrotic cells. Our aim was to determine the significance of HMGB1 in the natural history of diabetes in non-obese diabetic (NOD) mice. We observed that the rate of HMGB1 expression in the cytoplasm of islets was much greater in diabetic mice compared with non-diabetic mice. The majority of cells positively stained for toll-like receptor 4 (TLR4) were βcells; few α cells were stained for TLR4. Thus, we examined the effects of anti-TLR4 antibodies on HMGB1 cell surface binding, which confirmed that HMGB1 interacts with TLR4 in isolated islets. Expression changes in HMGB1 and TLR4 were detected throughout the course of diabetes. Our findings indicate that TLR4 is the main receptor on β cells and that HMGB1 may signal via TLR4 to selectively damage β cells rather than αcells during the development of type 1 diabetes mellitus.
Large-scale Field Tests of the Performance of Geogrid-reinforced Piled Embankment over Soft Soil
Gang Wang,Xianwei Zhang,Xinyu Liu,Zhixiong Chang,Zhihai Liu 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2
When constructing superstructures on soft soils, geogrid-reinforced embankments and pile-supported embankments are used widely to improve the soft foundation and prevent issues including excessive settlements and large lateral displacements, so it is crucial to understand and evaluate their performance systematically. This study focuses on a case of piled embankments for a motor-racing circuit under construction in China. Two large-scale field tests of pile-supported embankments with and without reinforcement were carried out to investigate the effect of geosynthetic reinforcement on the improvement of embankments. During long-term monitoring, instruments in the test sites measured earth pressures, settlements, lateral displacements, and pore water pressures, and the performances of the reinforced or unreinforced embankments were examined. Test results from the two test sites under similar loading are compared, and it is concluded that the geosynthetic-reinforcement considerably influences the improvement of load transfer and diminishment of total and differential settlements. The lateral restraint provided by the geogrids also reduced the lateral soil displacement and the influence of subsoil depth. The smaller excessive pore water pressures in the site with reinforcement were associated with the enhanced load transfer due to the geogrids. This study provides an important reference for the design and construction of this circuit and other related projects.
Point-to-point motions control of an electromagnetic direct-drive gas valve
Jiayu Lu,Siqin Chang,Liang Liu,Xinyu Fan 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1
Electromagnetic linear actuators are widely used as drives in gas regulating valve, which is the key factor to determine the performance of the gas valve. In order to improve the performance of Electromagnetic direct-drive gas valve (EMDGV), a compound controller for non-repetitive point-to-point motions was proposed in this paper, combining with the advantages of Active disturbance rejection control (ADRC), Time-optimal control (TOC) and Sliding mode control (SMC). Simulation and experimental results show that the proposed method has the characteristics of fast response and high precision, and can achieve soft landing control. The response time is less than 8 ms, the control accuracy is 0.02 mm, and the seating velocity is within 0.04 m/s. The proposed method also has strong robustness to actuator parameter variation and good anti-disturbance ability.
Wang Tingting,Yang Yang,Liu Xinyue,Deng Jiajun,Wu Junqi,Hou Likun,Wu Chunyan,She Yunlang,Sun Xiwen,Xie Dong,Chen Chang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.4
Objective: To investigate the association between CT imaging features and survival outcomes in patients with primary invasive mucinous adenocarcinoma (IMA). Materials and Methods: Preoperative CT image findings were consecutively evaluated in 317 patients with resected IMA from January 2011 to December 2015. The association between CT features and long-term survival were assessed by univariate analysis. The independent prognostic factors were identified by the multivariate Cox regression analyses. The survival comparison of IMA patients was investigated using the Kaplan-Meier method and propensity scores. Furthermore, the prognostic impact of CT features was assessed based on different imaging subtypes, and the results were adjusted using the Bonferroni method. Results: The median follow-up time was 52.8 months; the 5-year disease-free survival (DFS) and overall survival rates of resected IMAs were 68.5% and 77.6%, respectively. The univariate analyses of all IMA patients demonstrated that 15 CT imaging features, in addition to the clinicopathologic characteristics, significantly correlated with the recurrence or death of IMA patients. The multivariable analysis revealed that five of them, including imaging subtype (p = 0.002), spiculation (p < 0.001), tumor density (p = 0.008), air bronchogram (p < 0.001), emphysema (p < 0.001), and location (p = 0.029) were independent prognostic factors. The subgroup analysis demonstrated that pneumonic-type IMA had a significantly worse prognosis than solitary-type IMA. Moreover, for solitary-type IMAs, the most independent CT imaging biomarkers were air bronchogram and emphysema with an adjusted p value less than 0.05; for pneumonic-type IMA, the tumors with mixed consolidation and ground-glass opacity were associated with a longer DFS (adjusted p = 0.012). Conclusion: CT imaging features characteristic of IMA may provide prognostic information and individual risk assessment in addition to the recognized clinical predictors.
Baohua Wang,Rong Fu,Mi Zhang,Zhenqian Ding,Lei Chang,Xinyu Zhu,Yafeng Wang,Baoxiang Fan,Wuwei Ye,Youlu Yuan 한국유전학회 2015 Genes & Genomics Vol.37 No.8
A methylation-sensitive amplification polymorphism method based on capillary electrophoresis was used to analyze DNA methylation levels in three cotton accessions, two salt-tolerant accessions CCRI 35 and Zhong 07 and one salt-sensitive accession CCRI 12. Many categories of DNA methylation happened in the three cotton accessions under salt treatment, including hypermethylation, hypomethylation, and other patterns. Hypermethylation happened at a significantly higher rate than that of hypomethylation in salt-tolerant accessions CCRI 35 and Zhong 07. On the contrary, in salt-sensitive accession CCRI 12, hypomethylation happened at a significantly higher rate than that of hypermethylation. In general, the global DNA methylation level significantly increased under salt stress in both salt-tolerant accessions CCRI 35 and Zhong 07, whereas there was no significant difference in the salt-sensitive accessions CCRI 12. Our results suggested that salt-tolerant cotton might have a mechanism of increasing the methylation level when responding to salt stress; the increase of the global level of DNA methylation and also different methylation patterns might play important roles in tolerance to salt stress in cotton. Some interesting genes were found through cloning and analysis of differently methylated DNA sequences, which might contribute to salt tolerance in cotton.