Effect of Trichokonins on the Growth Characteristics of Paeonia ostii ``Fengdan Seedling Roots

( Peng-fei Chu ),( Xing Cao ),( Zhen-jing Yang ),( Xiu-sheng Zhang ),( Yong-ji Piao ),( Hyun-ju Jo ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.8

The growth characteristics of Paeonia ostii ``Fengdan`` seedlings roots in response to trichokonins-spray treatment were investigated in this study. One-year-old seedlings of P. ostii ``Fengdan`` were potted in plastic cups containing garden mold and grown under field conditions. The results showed that application of trichokonins significantly promoted root growth in P. ostii ``Fengdan`` seedlings. The total root projection area, total root surface area, total root volume, total root length, root number and root diameter of seedlings treated with 0.25 mg/L trichokonins were higher by 141.70, 116.59, 119.44, 55.97, 348.88 and 127.78%, respectively, than that of the control. Thus, the results supported the hypothesis that good growth condition for roots could directly improve their nutrient absorption and utilization efficiency, promoting plant growth and development.

A Novel Molecular Grading Model: Combination of Ki67 and VEGF in Predicting Tumor Recurrence and Progression in Non-invasive Urothelial Bladder Cancer

Chen, Jun-Xing,Deng, Nan,Chen, Xu,Chen, Ling-Wu,Qiu, Shao-Peng,Li, Xiao-Fei,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Purpose: To assess efficacy of Ki67 combined with VEGF as a molecular grading model to predict outcomes with non-muscle invasive bladder cancer (NMIBC). Materials: 72 NMIBC patients who underwent transurethral resection (TUR) followed by routine intravesical instillations were retrospectively analyzed in this study. Univariate and multivariate analyses were performed to confirm the prognostic values of the Ki67 labeling index (LI) and VEGF scoring for tumor recurrence and progression. Results: The novel molecular grading model for NMIBC contained three molecular grades including mG1 (Ki67 $LI{\leq}25%$, VEGF $scoring{\leq}8$), mG2 (Ki67 LI>25%, VEGF $scoring{\leq}8$; or Ki67 $LI{\leq}25%$, VEGF scoring > 8), and mG3 (Ki67 LI > 25%, VEGF scoring > 8), which can indicate favorable, intermediate and poor prognosis, respectively. Conclusions: The described novel molecular grading model utilizing Ki67 LI and VEGF scoring is helpful to effectively and accurately predict outcomes and optimize personal therapy.

Application of Near-Infrared Fluorescence Imaging with Indocyanine Green in Totally Laparoscopic Distal Gastrectomy

Maoxing Liu,Jiadi Xing,Kai Xu,Peng Yuan,Ming Cui,Chenghai Zhang,Hong Yang,Zhendan Yao,Nan Zhang,Fei Tan,Xiangqian Su 대한위암학회 2020 Journal of gastric cancer Vol.20 No.3

aPurpose: Recently, totally laparoscopic gastrectomy has been gradually accepted by surgeons worldwide for gastric cancer treatment. Complete dissection of the lymph nodes and the establishment of the surgical margin are the most important considerations for curative gastric cancer surgery. Previous studies have demonstrated that indocyanine green (ICG)-traced laparoscopic gastrectomy significantly improves the completeness of lymph node dissection. However, it remains difficult to identify the tumor location intraoperatively for gastric cancers that are staged ≤T3. Here, we investigated the feasibility of ICG fluorescence for lymph node mapping and tumor localization during totally laparoscopic distal gastrectomy. Materials and Methods: Preoperative and perioperative data from consecutive patients with gastric cancer who underwent a totally laparoscopic distal gastrectomy were collected and analyzed. The patients were categorized into the ICG (n=61) or the non-ICG (n=75) group based on whether preoperative endoscopic mucosal ICG injection was performed. Results: The ICG group had a shorter operation time and less intraoperative blood loss. Moreover, significantly more lymph nodes were harvested in the ICG group than the non-ICG group. No pathologically positive margin was found and there was no significant difference in either the proximal or distal surgical margins between the 2 groups. Conclusions: Near-infrared fluorescence imaging with ICG can be successfully used in totally laparoscopic distal gastrectomy, and it contributes to both the completeness of D2 lymph node dissection and confirmation of the gastric transection line. Well-designed prospective randomized studies are needed in the future to fully validate our findings.

Branched polymeric prodrug/programmed cell death 4 complexes for combinational cancer therapy

Yu‑Jing He,Lei Xing,Peng‑Fei Cui,Jia‑Liang Zhang,Jian‑Bin Qiao,Cheng‑Qiong Luo,Ge Jiang,Hu‑Lin Jiang 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.2

Here, we demonstrate a co-delivery system constructed by integrating chemotherapeutic molecules into branched polymeric prodrug which can condense nucleic acids. Demethylcantharidate (DCA) was chosen as a model drug and premodified through nucleophilic substitution reaction by its two carboxylic groups with allyl chloride. The synthesized intermediate (DCA-dially) was then used to polymerize with tris (2-aminoethyl) amine (TAEA) through progressively ammonolysis reaction. The obtained poly (DCA-alt-TAEA) (DCAT) was used to pack PDCD4 into spherical-like nanoparticles through electrostatic interaction. Gel retardation assays implied that DCAT could integrate DNA at the weight ratio of 1 and protect it from digestion by nuclease. Acid-base titration experiments showed that DCAT obtained preferable buffering capability which was beneficial for the endosomal escape of DCAT/PDCD4 complexes. Cellular tests involving gene transfection efficiency and cytotoxicity indicated that DCA and PDCD4 co-delivered by the complexes significantly and synergistically suppressed the viability of SMMC-7721 cells. These results suggest that integrating chemotherapeutic molecules into nucleic acid-packing polymeric prodrug as cationic polymer/PDCD4 complexes is a highly efficient way to co-deliver chemotherapeutic drugs and nucleic acids for cancer therapy.

Upregulation and biological function of transmembrane protein 119 in osteosarcoma

Zhen-Huan Jiang,Jun Peng,Hui-Lin Yang,Xing-Li Fu,Jin-Zhi Wang,Lei Liu,Jian-Nong Jiang,Yong-Fei Tan,Zhi-Jun Ge 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Osteosarcoma is suggested to be caused by genetic and molecular alterations that disrupt osteoblast differentiation. Recent studies have reported that transmembrane protein 119 (TMEM119) contributes to osteoblast differentiation and bone development. However, the level of TMEM119 expression and its roles in osteosarcoma have not yet been elucidated. In the present study, TMEM119 mRNA and protein expression was found to be up-regulated in osteosarcoma compared with normal bone cyst tissues. The level of TMEM119 protein expression was strongly associated with tumor size, clinical stage, distant metastasis and overall survival time. Moreover, gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset revealed TMEM119 expression in osteosarcoma tissues to be positively correlated with cell cycle, apoptosis, metastasis and TGF-β signaling. We then knocked down TMEM119 expression in U2OS and MG63 cells using small interfering RNA, which revealed that downregulation of TMEM119 could inhibit the proliferation of osteosarcoma cells by inducing cell cycle arrest in G0/G1 phase and apoptosis. We also found that TMEM119 knockdown significantly inhibited cell migration and invasion, and decreased the expression of TGF-β pathway-related factors (BMP2, BMP7 and TGF-β). TGF-β application rescued the inhibitory effects of TMEM119 knockdown on osteosarcoma cell migration and invasion. Further in vitro experiments with a TGF-β inhibitor (SB431542) or BMP inhibitor (dorsomorphin) suggested that TMEM119 significantly promotes cell migration and invasion, partly through TGF-β/BMP signaling. In conclusion, our data support the notion that TMEM119 contributes to the proliferation, migration and invasion of osteosarcoma cells, and functions as an oncogene in osteosarcoma.

중국 수이둥거우(水洞沟)유적의 성격과 특징

최철민(Cheolmin CHOI):펑페이(彭菲)(Fei PENG),까오싱(高星)(Xing GAO) 한국구석기학회 2017 한국구석기학보 Vol.- No.35

본고는 중국 영하회족자치구(宁夏回族自治区)에 위치하는 수이둥거우(水洞沟,SDG) 유적의 특징을 고찰하고 더 나아가 중국 북방 지역 후기 구석기시대를 이해하는 것이 목적이다. 수이둥거우 유적은 1923년 중국에서 처음 확인된 구석기 유적으로 단구, 황토 고원과 같은 지형 환경, 석기 제작 기술 및 기원 그리고 수렵채집민 이동 및 환경 적응 등 다양한 연구가 이루어지고 있다. 지금까지 수이둥거우 유적군에서 모두 12개 지점이 발굴조사 되었다. 과거에는 처음 확인된 1지점을 중심으로 연구되었으나 최근에는 2지점에서 매년 발굴조사가 진행되고 있으며 이에 따라 새로운 자료가 확인되고 있다. 그 외 지점들은 조사기간 및 면적에 있어서 소규모 조사가 진행되었으며 1, 2지점과 비교하면 자료가 빈약하다. 수이둥거우 유적의 단구 및 지층 연구에 있어서 연구자마다 의견 차이가 존재한다. 하지만 유적군이 제2단구에 분포한다는 것과 플라이스토세 후기 늦은 시기에 형성된 황토층에서 후기 구석기시대 유물이 출토되고 홀로세 시기에 형성된 하천 퇴적층에서 신석기시대 유물층이 확인된다는 것에는 이견이 없다. 반면 각 지점마다 석기 유형의 차이가 존재하는데 1, 9, 12지점에서는 돌날 또는 잔돌날 유형이 나타나고 그 외 지점에서는 작고 불규칙적인 격지, 잔손질 도구와 같은 중국 북방 지역의 후기 구석기시대 특징이 나타난다. 수이둥거우 유적군에서는 이러한 석기 유물뿐 만 아니라 다량의 타조알조각을 투공한 장식품도 확인되며 고인류가 불을 사용한 유구 주변에서는 동물뼈가 다량으로 출토되기도 한다. 유적군 연대값에 있어서 측정 방법과 표본의 차이로 불확실한 점은 존재하지만 하층 유물층이 후기 구석기시대 유물층이라는 것에는 논쟁이 없다. 수이둥거우 유적군의 대략적인 유물층 시기는 3만 5천 년 전에서 2만 년 전으로 추정된다. 최근 한반도와 북중국 지역에서 새로운 돌날 및 잔돌날 관련 유적이 꾸준히 확인되고 있다. 하지만 한반도에서 돌날 제작 기술의 기원 및 현생인류의 확산을 직접 설명할 수 있는 증거가 많지 않다. 따라서 중국에서 확인되는 구석기시대 자료를 활용한다면 넓은 공간적 배경에서 두 지역의 비교 연구가 가능해지고 이에 따라 당시의 기후 환경, 수렵채집민의 이동성, 유물 분포 범위 등과 같은 종합적인 분석이 가능해진다. The purpose of this article is to examine the characteristics of Shuidonggou site, which is located at the Ningxia Autonomous Region of China, and to understand the Upper Paleolithic in North China. The Shuidonggou site was first discovered in China in 1923, and various researches have been carried out to date including the lithic manufacturing techniques, geomorphological environment such as loess plateaus, mobility of hunter-gathers; and adaptation to environment. So far, all 12 localities have been excavated. In the past, SDG1(locality 1) was given focus, but recently, excavation has been conducted mainly in SDG2(locality 2) every year. Although there is a disagreement among the topographical researchers of the Shuidonggou site, there is no dispute as to how the sites were distributed on terrace 2(T2), how the Upper Paleolithic artifacts were excavated in the loess layers in the late Pleistocene period, and how the Neolithic artifacts were found in the river sediments during the Holocene period. There is a different lithic assemblage for each locality. Blades and microblades assemblages appear in locality 12. In other localities, the features of the Upper Paleolithic of North China appear such as small, irregular flakes, and retouch tools. Not only these lithic artifacts, but ornaments made by piercing a large amount of ostrich eggshells were also identified, and animal bones were also excavated in large quantities around functional areas where hunter-gatherers used hearth. There is uncertainty in regard to its period due to the difference in measurement methods and materials in age-dating techniques, but there is no controversy that the lower cultural layer is considered remains of the Upper Paleolithic. The approximate cultural layer dating is estimated to be 35,000 to 20,000 years ago. Despite blade and microblade artifacts have been found in North China. There is not much evidence to explain the origins of blade production technology and the proliferation of modern humans in the Korean Peninsula. Therefore, the use of Paleolithic data in China makes it possible to carry out comparative studies on a wide spatial background, and enables a comprehensive analysis on climate environment, mobility of hunter-gatherers, distribution range of artifacts, etc.

Security Analysis of Speech Perceptual Hashing Authentication Algorithm

Zhang Qiu-yu,Ren Zhan-wei,Xing Peng-fei,Huang Yi-bo,Yu Shuang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.1

Speech perceptual hashing authentication algorithm is an efficient method for content integrity authentication and identity authentication. But the algorithm becomes transparent under the principle of Kerckhoffs which makes the algorithm unsafe. In this paper, the algorithm is encrypted under the principle of Kerckhoffs to protect the security of the algorithm. Then the specific link that won’t affect the performance of the algorithm is identified through the experiments. Next, this paper analyzes the security of the encrypted algorithm based on the concept of Shannon unicity distance. The unicity distance is figured out finally based on the experiments. That is to say, the algorithm loses its security even though the algorithm has been encrypted. Under this circumstances, the most important thing is to continue keeping the safety of the algorithm. Therefore, this paper proposed an efficient random secret key method to guarantee the safety of the algorithm after the unicity distance is figured out.

Prognostic Significance of Tumor Mutation Burden among Patients with Non-small Cell Lung Cancer Who Received Platinum-based Adjuvant Chemotherapy: An Exploratory Study

Wei-Xi Shen,Guang-Hua Li,Yu-Jia Li,Peng-Fei Zhang,Jia-Xing Yu,Di Shang,Qiu-Shi Wang 대한암예방학회 2023 Journal of cancer prevention Vol.28 No.4

This study aimed to investigate the prognostic significance of tumor mutation burden (TMB) among patients with non-small cell lung cancer (NSCLC) who received platinum-based adjuvant chemotherapy. Tumor tissue specimens after surgical resection were collected for DNA extraction. Somatic mutation detection and TMB analysis were conducted using next-generation sequencing (NGS). Recurrence status of the patients was assessed in the hospital during the adjuvant chemotherapy period, and long-term survival data of patients were obtained by telephone follow-up. Univariate analysis between TMB status and prognosis was carried out by survival analysis. A retrospective review of 78 patients with non-squamous NSCLC who received platinum-based adjuvant chemotherapy showed a median disease-free survival of 3.6 years and median overall survival (OS) of 5.3 years. NGS analysis exhibited that the most common mutated somatic genes among the 78 patients were tumor suppressor protein p53 (TP53), epidermal growth factor receptor, low-density lipoprotein receptor related protein 1B, DNA methyltransferase 3 alpha and FAT atypical cadherin 3, and their prevalence was 56.4%, 48.7%, 37.2%, 30.7%, and 25.6%, respectively. TMB status was divided into TMB-L (≤ 4.5/Mb) and TMB-H (> 4.5/Mb) based on the median TMB threshold. Relevance of TMB to prognosis suggested that the median OS of patients with TMB-L was significantly longer than that of patients with TMB-H (NR vs. 4.6, P = 0.014). Higher TMB status conferred a worse implication on OS among patients with non-squamous NSCLC who received platinum-based adjuvant chemotherapy.

Long-term Survivals, Toxicities and the Role of Chemotherapy in Early-Stage Nasopharyngeal Carcinoma Patients Treated with Intensity-modulated Radiation Therapy: A Retrospective Study with 15-year Follow-up

Lin Wang,Jingjing Miao,Huageng Huang,Boyu Chen,Xiao Xiao,Manyi Zhu,Yingshan Liang,Weiwei Xiao,Shaomin Huang,Yinglin Peng,Xiaowu Deng,Xing Lv,Weixiong Xia,Yanqun Xiang,Xiang Guo,Fei Han,Chong Zhao 대한암학회 2022 Cancer Research and Treatment Vol.54 No.1

Purpose This study was aimed to investigate long-term survivals and toxicities of early-stage nasopharyngeal carcinoma (NPC) in endemic area, evaluating the role of chemotherapy in stage II patients. Materials and Methods Totally 187 patients with newly diagnosed NPC and restaged American Joint Committee on Cancer/ International Union Against Cancer 8th T1-2N0-1M0 were retrospectively recruited. All received intensity-modulated radiotherapy (IMRT)±chemotherapy (CT) from 2001 to 2010. Results With 15.7-year median follow-up, 10-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), disease-specific survival (DSS), and overall survival (OS) were 93.3%, 93.5%, 92.9% and 88.2%, respectively. Multivariable analyses showed cervical lymph nodes positive and pre-treatment prognostic nutritional index ≥ 52.0 could independently predict DMFS (p=0.036 and p=0.011), DSS (p=0.014 and p=0.026), and OS (p=0.002 and p < 0.001); Charlson comorbidity index < 3 points could predict DSS (p=0.011); age > 45 years (p=0.002) and pre-treatment lactate dehydrogenase ≥ 240 U/L (p < 0.001) predicted OS. No grade 4 late toxicity happened; grade 3 late toxicities included subcutaneous fibrosis (4.3%), deafness or otitis (4.8%), skin dystrophy (2.1%), and xerostomia (1.1%). No differences on survivals were shown between IMRT+CT vs. IMRT alone in stage II patients, even in T2N1M0 (p > 0.05). Unsurprising, patients in IMRT+CT had more acute gastrointestinal reaction, myelosuppression, mucositis, late ear toxicity, and cranial nerve injury (all p < 0.05) than IMRT alone group. Conclusion Superior tumor control and satisfying long-term outcomes could be achieved with IMRT in early-stage NPC with mild late toxicities. As CT would bring more toxicities, it should be carefully performed to stage II patients.