Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

XiaoYing He,LiBing Ma,Xiao-ning He,Wan-tong Si,Yue-Mao Zheng 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.2

Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%, respectively) and the transgenic group (80.8%, 26.5% and 53.4%); however, they were significantly higher than the bMGEs group (71.2%, 12.8% and 14.8%), (p < 0.05). We confirmed that hTERT-bMGEs could serve as effective target cells for improving development of somatic cell cloned cattle embryos.

Energy Efficient Sequential Sensing in Multi-User Cognitive Ad Hoc Networks: A Consideration of an ADC Device

Xiaoying Gan,Miao Xu,He Li 한국통신학회 2012 Journal of communications and networks Vol.14 No.2

Cognitive networks (CNs) are capable of enabling dynamic spectrum allocation, and thus constitute a promising technology for futurewireless communication.Whereas, the implementation of CN will lead to the requirement of an increased energyarrival rate, which is a significant parameter in energy harvesting design of a cognitive user (CU) device. A well-designed spectrumsensing scheme will lower the energy-arrival rate that is required and enable CNs to self-sustain, which will also help alleviate global warming. In this paper, spectrum sensing in a multi-user cognitive ad hoc network with a wide-band spectrum is considered. Based on the prospective spectrum sensing, we classify CN operation into two modes: Distributed and centralized. In a distributed network,each CU conducts spectrum sensing for its own data transmission,while in a centralized network, there is only one cognitive cluster header which performs spectrum sensing and broadcasts its sensing results to other CUs. Thus, a wide-band spectrum that is divided into multiple sub-channels can be sensed simultaneously in a distributed manner or sequentially in a centralized manner.We consider the energy consumption for spectrum sensing only of an analog-to-digital convertor (ADC). By formulating energy consumption for spectrum sensing in terms of the sub-channel sampling rate and whole-band sensing time, the sampling rate and whole-band sensing time that are optimal for minimizing the total energy consumption within sensing reliability constraints are obtained. A power dissipation model of an ADC, which plays an important role in formulating the energy efficiency problem, is presented. Using AD9051 as an ADC example, our numerical results show that the optimal sensing parameters will achieve a reduction in the energy-arrival rate of up to 97.7% and 50% in a distributed and a centralized network, respectively, when comparing the optimal and worst-case energy consumption for given system settings.

Coupled Discrete Crack and Porous Media Model for Hydraulic Fractures using the XFEM

Bo He,Xiaoying Zhuang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.3

A hydromechanical model for investigating fluid flow in the fractured porous media is presented in this study. The hydromechanical coupling equations are derived from the mass and momentum balance equation for the saturated porous media. The extended finite element method is employed to model the discontinuity for fluid flow and cracks inside the porous media. The Newton-Raphson method is utilized for solving the nonlinear coupling equation with an implicit time integration scheme. Finally, examples are presented to demonstrate the effectiveness of the presented model. Fracture propagation in the porous media under the influence of the preexisted pressurized zone is also studied. It is found that the cracks and preexisted pressurized region have a significant impact on the fluid flow and deformation patterns.

Energy Efficient Sequential Sensing in Multi-User Cognitive Ad Hoc Networks: A Consideration of an ADC Device

Gan, Xiaoying,Xu, Miao,Li, He The Korea Institute of Information and Commucation 2012 Journal of communications and networks Vol.14 No.2

Cognitive networks (CNs) are capable of enabling dynamic spectrum allocation, and thus constitute a promising technology for future wireless communication. Whereas, the implementation of CN will lead to the requirement of an increased energy-arrival rate, which is a significant parameter in energy harvesting design of a cognitive user (CU) device. A well-designed spectrum-sensing scheme will lower the energy-arrival rate that is required and enable CNs to self-sustain, which will also help alleviate global warming. In this paper, spectrum sensing in a multi-user cognitive ad hoc network with a wide-band spectrum is considered. Based on the prospective spectrum sensing, we classify CN operation into two modes: Distributed and centralized. In a distributed network, each CU conducts spectrum sensing for its own data transmission, while in a centralized network, there is only one cognitive cluster header which performs spectrum sensing and broadcasts its sensing results to other CUs. Thus, a wide-band spectrum that is divided into multiple sub-channels can be sensed simultaneously in a distributed manner or sequentially in a centralized manner. We consider the energy consumption for spectrum sensing only of an analog-to-digital convertor (ADC). By formulating energy consumption for spectrum sensing in terms of the sub-channel sampling rate and whole-band sensing time, the sampling rate and whole-band sensing time that are optimal for minimizing the total energy consumption within sensing reliability constraints are obtained. A power dissipation model of an ADC, which plays an important role in formulating the energy efficiency problem, is presented. Using AD9051 as an ADC example, our numerical results show that the optimal sensing parameters will achieve a reduction in the energy-arrival rate of up to 97.7% and 50% in a distributed and a centralized network, respectively, when comparing the optimal and worst-case energy consumption for given system settings.

Somatic cell reprogrammed by oocyte: process and barricade

LiBing Ma,XiaoYing He,FengMei Wang,Teng Cheng,XiYu Liu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.3

Somatic cell nuclear transfer (SCNT) is a technology in which a somatic nucleus is transferred into thecytoplasm of a matured oocyte – reconstructed embryos have the capacity to develop to term. Why somaticcells can be reprogrammed by oocytes – the answer for this question must exist in the cytoplasm of maturedoocytes. In this review, totipotent characters of matured oocytes were discussed, which may confer maturedoocytes with the capacity to reprogram somatic nucleus. Moreover, the procedure of SCNT also makes apossibility for somatic nucleus to be reprogrammed by oocytes. Compared with fertilized embryos, embryosderived from SCNT exhibit low developmental ability; the barricades in reprogramming process and theirpossible reasons were also discussed. This review maybe can benefit the mechanism research of SCNTtechnology and can make contribution for improving the efficiency of this technology.

Analysis and Accurate Modeling of a Flyback Converter on Conducted EMI

Weichang Cheng,Xiaoying He,Shen Xu,Weifeng Sun 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

In this paper an accurate EMI model of a 5W flyback converter based on discrete devices modeling is presented. To improve the accuracy of EMI model, passive devices, active devices, power IC and the parameters of PCB are all taken into consideration. The simulation results show that, errors of main system-level parameters can be controlled in 5% and errors at the EMI peak values can be controlled in ± 5dB. Based on the proposed model, the existence of NIDM noise is verified and the impacts of parasitic parameters on PCB in this flyback converter are analyzed. The model-based analysis of EMI agrees well with the theory and measurement.

Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis

Mengchao Ding,Xiaoying Kong,Weiyan Chen,Lei Yan,He Huang,Zunzhou Lv,Peng Jiang,Ali Mu,Congcong Huang,Jinsheng Shi 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Single cancer starvation therapy (ST) strategy can’t achieve satisfactory anti-tumor effect, mainly due tothe diversified nutritional sources of tumor cells. Herein, CuS@Axitinib-SiO2@2-Deoxy-D-glucose(2-DG)-CaCO3-RGD nanoparticles (CADCR NPs) were prepared for three-pathway blocking for efficient starvationtherapy as well as reinforced photothermal therapy (PTT) and chemodynamic therapy (CDT). AfterCADCR NPs were targeted to tumor cells, CaCO3 was ruptured in the acidic environment, releasingCa2+ to chelate glutamine and cutting off the glutamine metabolic pathway of the tumor. 2-DG was alsoreleased from mesoporous SiO2 and restrained the glycolytic pathway of tumor cells. In addition, underthe thermal stimulus of near-infrared irradiation, axitinib was released from CuS NPs, which inhibited theproliferation of tumor blood vessels, ultimately inhibiting the aerobic respiratory pathway of tumor cells. Interestingly, CADCR NPs also showed potential to reshape the tumor microenvironment (TME) and promotedthe transformation of macrophages from M2 to M1 type, increasing the expression of CD8+ T cellsin the tumor site. In conclusion, CADCR NPs achieve severe tumor starvation by simultaneously interferingwith three energy metabolic pathways, and further enhance tumor treatment with the aid of PTT,CDT, and TME improvement, which exhibits great potential for clinical cancer therapy.

Heterologous Expression of a Gibberellin 2-Oxidase Gene from Arabidopsis thaliana Enhanced the Photosynthesis Capacity in Brassica napus L.

Bo Zhou,Dan Peng,Jianzhong Lin,Xingqun Huang,Wusheng Peng,Reqing He,Ming Guo,Dongying Tang,Xiaoying Zhao,Xuanming Liu 한국식물학회 2011 Journal of Plant Biology Vol.54 No.1

Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing the amount of bioactive GA and hence reduced the plant height. As anticipated, the transgenic plant exhibited dwarf phenotype. Importantly, compared with the wild type, the transgenic plants had delayed the seed germination, increased the chlorophyll content (28.7–36.3%) and photosynthesis capacity (14.3–18.7%) in a single leaf. At the same time, the photosynthesis capacity of the whole plants was significantly enhanced (35.7–48.6%) due to the extra leaves and branches.