Genetic characterization of bovine viral diarrhea virus strains in Beijing, China and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle

Xiao Gang Weng,Quan Jiang Song,Qiong Wu,Ming Chao Liu,Meng Ling Wang,Jiu Feng Wang 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4

To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5’-untranslated region (5’-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-a (IFN-a), IFN-b, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-a, and IFN-b mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.

Grouting to Prevent Sulfate Corrosion on Coal Mine Shaft

Gang-Gang Xu,Shuan-Cheng Gu,Xiao-Dong Wang,Hai Wang,Shi-Bin Zhu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11

In the area of high sulfate concentration in China, the problem of sulfate corrosion in coal mine shaft is increasingly prominent. Currently, shaft repair methods are limited to diversion, interception, and backwall grouting. However, after conventional cement stabilization, shafts still must contend with poor durability and weak resistance to sulfate corrosion. To solve these problems, this study combined theoretical research, laboratory tests, and field tests to reveal the corrosion mechanism of shafts, explored novel anti-sulfate corrosion grout, and built a model for the migration of sulfate ions (SO42−) in strata and shafts. According to the results of this study, shaft corrosion was a process of continuous penetration towards the concrete interior following a cycle of concrete compaction, expansion, and cracking. Laboratory tests show that the specimens of ordinary Portland cement mortar with 20 – 30% fly ash not only have good long-term strength, but also have a significantly improved resistance capacity to sulfate solution. Based on the laboratory test results, the HSR42.5 mixed with 20% fly ash was used for wall grouting, and the water-cement ratio of the slurry was 1:1. After wall grouting, the water inflow was effectively controlled in the grouting area in the main shaft, auxiliary shaft, and air shaft, reducing from 18.5 m3/h, 20.9 m3/h, and 10.0 m3/h to 3.5 m3/h, 4.6 m3/h, and 3.2 m3/h, respectively. Moreover, after nearly three years of continuous monitoring, the water inflow did not show any significant increase in the shafts. Based on the migration law of SO42− in concrete, a shaft geological model and a salt solution migration model after grouting reinforcement under sulfate corrosion conditions were constructed. In addition, the analysis of the migration law of sulfate ion in the shaft and grouting reinforcement formation revealed that the service period of the grouting shaft can be extended for approximately 6 – 8 years.

Inhibition of RIPK1-dependent regulated acinar cell necrosis provides protection against acute pancreatitis via the RIPK1/NF- κB/AQP8 pathway

Gang Wang,Peng-yu Duan,Yuan Ma,Xi-na Li,Feng-zhi Qu,Liang Ji,Xiao-yu Guo,Wang-jun Zhang,Fan Xiao,Le Li,Ji-sheng Hu,Bei Sun 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Currently, preliminary results have confirmed the existence of receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis of pancreatic acinar cells during early acute pancreatitis (AP), which might be a potential target for the effective regulation of necroinflammatory injury. However, the exact effect of receptor-interacting protein kinase 1 (RIPK1)-dependent regulated acinar cell necrosis on AP is still uncertain. In our study, we first explored the changes in the degree of local and systemic inflammation in AP rats when the activation of acinar cell RIPK1 was inhibited. The RIPK1 inhibitor Nec-1 was used to treat rats, and the levels of related inflammatory markers, necrosis indicators and apoptotic indicators were measured. Changes in pancreatic nuclear factor κB (NF-κB) and aquaporin 8 (AQP8) expression were noted. Next, the expression of AQP8 in AR42J cells was inhibited, and the degree of cell necrosis and inflammatory damage was found to be significantly reduced. Most importantly, we demonstrated that the RIPK1/NF-ĸB/AQP8 axis might be a potential regulatory pathway mediating RIPK1-dependent regulated acinar cell necrosis in early AP. Finally, we used the NF-κB inhibitor PDTC and Nec-1 to treat rats in different groups and measured the degree of pathological pancreatic injury, the activation of RIPK1, and the expression of NF-κB and AQP8. In summary, we hypothesized that there might be a RIPK1/NF-ĸB/AQP8 pathway controlling RIPK1-dependent regulated necrosis of acinar cells in AP, which might be a promising therapeutic target against AP-related injury.

Exploring the role and mechanisms of diallyl trisulfide and diallyl disulfide in chronic constriction-induced neuropathic pain in rats

( Gang Wang ),( Yan Yang ),( Chunfeng Wang ),( Jianzhong Huang ),( Xiao Wang ),( Ying Liu ),( Hao Wang ) 대한통증학회 2020 The Korean Journal of Pain Vol.33 No.3

Background: Garlic oil is a rich source of organosulfur compounds including diallyl disulfide and diallyl trisulfide. There have been studies showing the neuroprotective actions of these organosulfur compounds. However, the potential of these organosulfur compounds in neuropathic pain has not been explored. The present study was aimed at investigating the pain attenuating potential of diallyl disulfide and diallyl trisulfide in chronic constriction injury (CCI)-induced neuropathic pain in rats. The study also explored their pain-attenuating mechanisms through modulation of H₂S, brain-derived neurotrophin factor (BDNF) and nuclear factor erythroid 2-related factor 2 (Nrf2). Methods: The rats were subjected to CCI injury by ligating the sciatic nerve in four places. The development of neuropathic pain was measured by assessing mechanical hyperalgesia (Randall-Selittotest), mechanical allodynia (Von Frey test), and cold allodynia (acetone drop test) on 14th day after surgery. Results: Administration of diallyl disulfide (25 and 50 mg/kg) and diallyl trisulfide (20 and 40 mg/kg) for 14 days led to a significant reduction in pain in CCI-subjected rats. Moreover, treatment with these organosulfur compounds led to the restoration of H₂S, BDNF and Nrf2 levels in the sciatic nerve and dorsal root ganglia. Coadministration of ANA-12 (BDNF blocker) abolished pain attenuating actions as well as BDNF and the Nrf2 restorative actions of diallyl disulfide and diallyl trisulfide, without modulating H₂S levels. Conclusions: Diallyl disulfide and diallyl trisulfide have the potential to attenuate neuropathic pain in CCI-subjected rats possibly through activation of H₂S-BDNF-Nrf2 signaling pathway.

Effect of Catalytic Properties on the Thermal Loads and Radiation Characteristics for Re-entry Capsules

Song Xiao-Xiao,Shuai Zhang,Gang Wang,Zhang Ji-Fa,Zheng Yao 한국항공우주학회 2024 International Journal of Aeronautical and Space Sc Vol.25 No.3

For the spectral characteristics of thermochemical nonequilibrium flow field gases and wall coating radiation, as well as the aerodynamic thermal loads of re-entry capsule Fire II, a finite-rate catalytic model combined with the multi-component chemical nonequilibrium N–S equation is developed. Based on this model, the effects of dissociation and recombination reactions of gas components on the wall coating temperature and heating flux are simulated. In addition, we investigate and analyze further the influence of the coating’s catalytic properties on the radiation spectral characteristic of the high-temperature nonequilibrium flow field and the wall coating radiation. The results show that the catalytic properties intensify the non-homogeneous recombination and exothermic processes near the wall surface. It results in a significant decrease in the number of component particles contributing to the radiative spectral intensity near the wall, which slightly reduces the flow field gas spectral radiation intensity. Meanwhile, the catalytic properties also increase the wall temperature and heating flux, significantly increasing the intensity of the wall radiation spectral in the near-infrared region.

Causes, Features, and Outcomes of Drug-Induced Liver Injury in 69 Children from China

( Yun Zhu ),( Yong Gang Li ),( Jia Bo Wang ),( Shu Hong Liu ),( Li Fu Wang ),( Yan Ling Zhao ),( Yun Feng Bai ),( Zhong Xia Wang ),( Jian Yu Li ),( Xiao He Xiao ) 대한소화기학회 2015 Gut and Liver Vol.9 No.4

Background/Aims: Drug-induced liver injury (DILI) is a frequent cause of pediatric liver disease; however, the data on DILI are remarkably limited. Methods: All 69 children hospitalized with DILI between January 2009 and December 2011 were retrospectively studied. Results: A total of 37.7% of the children had medical histories of respiratory infection. The clinical injury patterns were as follows: hepatocellular 89.9%, cholestatic 2.9%, and mixed 7.2%. Liver biopsies from 55 children most frequently demonstrated chronic (47.3%) and acute (27.3%) hepatitis. Hypersensitivity features, namely, fever (31.9%), rash (21.7%), and eosinophilia (1.4%), were found. Twenty-four children (34.8%) developed chronic DILI. Antibiotics (26.1%) were the most common Western medicines (WMs) causing DILI, and the major implicated herbs were Ephedra sinica and Polygonum multiflorum. Compared with WM, the children whose injuries were caused by Chinese herbal medicine (CHM) showed a higher level of total bilirubin (1.4 mg/dL vs 16.6 mg/dL, p=0.004) and a longer prothrombin time (11.8 seconds vs 17.3 seconds, p=0.012), but they exhibited less chronic DILI (2/15 vs 18/39, p=0.031). Conclusions: Most cases of DILI in children are caused by antibiotics or CHM used to treat respiratory infection and present with hepatocellular injury. Compared with WM, CHM is more likely to cause severe liver injury, but liver injury caused by CHM is curable. (Gut Liver 2015;9:525-533)

Long-Term of the Consecutively Monocultured Peanut Obviously Alters the Community Composition of Soil Nematodes in the Red Soil Region of Southern China

Xiao-Gang Li,Xing-Xiang Wang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Wang, Xiao-bo,Gao, Hui-Yuan,Hou, Bai-ling,Huangi, Jian,Xi, Rong-gang,Wu, Li-Jun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

School of Traditional Chinese Medicines, Shenyang Pharmaceutical University , Department of Pharmacy, Department of traditional Chinese MeNanoparticle realqar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibiter (z-DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3 poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In audition, NRP Increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor(SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.

Integrated Guidance and Control Design for the Near Space Interceptor

WANG Fei,LIU Gang,LIANG Xiao-Geng 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.2

Considering the guidance and control problem of the near space interceptor (NSI) during the terminal course, this paper proposes a three-channel independent integrated guidance and control (IGC) scheme based on the backstepping sliding mode and finite time disturbance observer (FTDO). Initially, the three-channel independent IGC model is constructed based on the interceptor-target relative motion and nonlinear dynamic model of the interceptor, in which the channel coupling term and external disturbance are regarded as the total disturbances of the corresponding channel. Then, the FTDO is introduced to estimate the target acceleration and control system loop disturbances, and the feed-forward compensation term based on the estimated values is employed to effectively remove the effect of disturbances in finite time. Subsequently, the IGC algorithm based on the backstepping sliding mode is also given to obtain the virtual control moment. Furthermore, a robust least-squares weighted control allocation (RLSWCA) algorithm is employed to distribute the previous virtual control moment among the corresponding aerodynamic fins and reaction jets, which also takes into account the uncertainty in the control effectiveness matrix. Finally, simulation results show that the proposed IGC method can obtain the small miss distance and smooth interceptor trajectories.

A Multi-group Multi-agent System Based on Reinforcement Learning and Flocking

Gang Wang,Jian Xiao,Rui Xue,Yongting Yuan 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.7

In this paper, we present an inter-group confrontation and intra-group cooperation method for a predator group and prey group, and construct a multi-group multi-agent system. We model the motion of the prey group using the flocking control algorithm. The prey group can cooperatively avoid predators and maintain the integrity of the group after the predators have been detected. The autonomous decision-making of the predator group is implemented based on the distributed reinforcement learning algorithm. To efficiently share the learning experience among agents in the predator group, a distributed cooperative reinforcement learning algorithm with variable weights is proposed to accelerate the convergence of the learning algorithm. Simulations show the feasibility of this proposed method.