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Pro‐apoptotic role of integrin β3 in glioma cells
Kim, Jongx2010,Heon,Zheng, Long Tai,Lee, Wonx2010,Ha,Suk, Kyoungho Blackwell Publishing Ltd 2011 Journal of Neurochemistry Vol.117 No.3
<P> <I>J. Neurochem.</I> (2011) <B>117</B>, 494–503.</P><P><B>Abstract</B></P><P>Malignant gliomas are the most destructive type of brain cancer. In order to gain a better understanding of the molecular mechanisms of glioma cell death and survival, we previously established an alkylating agent 1, 3‐bis(2‐chloroethyl)‐1‐nitrosourea (BCNU)‐resistant variant of C6 rat glioma cells. Proteomic analysis indicated a significant down‐regulation of integrin beta 3 (ITGB3) in the BCNU‐resistant C6R cells. Re‐expression of ITGB3 in C6R cells restored the BCNU sensitivity. In U87MG, U373MG, and T98G human glioma cells, there was a positive correlation between ITGB3 expression and the sensitivity to BCNU and etoposide, suggesting an important role of ITGB3 in glioma cell death. Over‐expression of ITGB3 cDNA significantly increased the sensitivity of the human glioma cells to the anticancer drug‐induced apoptosis. Nitric oxide showed an additive effect on the anticancer drug‐induced glioma cell death by increasing ITGB3 expression. Subsequent dissection of signaling pathways indicated that extracellular signal‐regulated kinase and unligated integrin‐mediated cell death pathway may be involved in the pro‐apoptotic role of ITGB3 in glioma cells. These results implicate ITGB3 in glioma cell death/survival and drug resistance.</P>
Yeo, Minx2010,Kyung,Liang, Zhe Long,Oh, Taejeong,Moon, Youngho,An, Sungwhan,Kim, Min Kyeong,Kim, Koon Soon,Shong, Minho,Kim, Jinx2010,Man,Jo, Young Suk Blackwell Publishing Ltd 2011 Clinical endocrinology Vol.75 No.4
<P><B>Summary</B></P><P><B>Context </B> Recently, tremendous efforts have been made towards the development of sensitive techniques to detect the BRAF<SUP>V600E</SUP> mutation in fine needle aspiration biopsy (FNAB) samples. However, newly developed quantitative and semi‐quantitative methods, such as dual‐priming oligonucleotide (DPO)‐based multiplex polymerase chain reaction (PCR), have the potential to generate false‐positive (FP) results.</P><P><B>Objectives </B> To eliminate the possibility of FP results, we generated a receiver operating characteristic (ROC) curve to investigate the diagnostic accuracy of pyrosequencing using quantitative data.</P><P><B>Design </B> Cytological diagnoses of 983 thyroid nodules were made according to the Bethesda System 2007. The BRAF<SUP>V600E</SUP> mutation was analysed by pyrosequencing, and statistical analyses were performed.</P><P><B>Results </B> Of the 983 nodules, 902 were adopted to evaluate the diagnostic value of pyrosequencing. The number of pathologically confirmed malignancies was 192, of which 182 were papillary thyroid cancer (PTC). By generating an ROC curve, we defined the optimal cut‐off value of the mutant allele peak as 5·95% (area under the curve, 0·849; sensitivity, 0·55; 1‐specificity, 0). When we applied this selective cut‐off value, the number of PTCs positive for BRAF<SUP>V600E</SUP> was 99 (54·4% of the total number of PTCs). With cytology alone, the diagnostic sensitivity and specificity of detecting malignancy were 71·2% and 100%, respectively. Pyrosequencing improved the diagnostic sensitivity from 71·2% to 78·5% (McNemar’s test, <I>P</I> < 0·001), without any change in the diagnostic specificity. When ‘suspicious for malignancy’ was considered a positive cytological outcome, pyrosequencing increased the diagnostic sensitivity of cytology from 95·8% to 96·9%; however, this improvement did not show statistical significance (McNemar’s test, <I>P</I> > 0·05).</P><P><B>Conclusions </B> Pyrosequencing is an effective method for detecting the BRAF<SUP>V600E</SUP> mutation in FNAB samples. By allowing the optimal cut‐off value to be determined, pyrosequencing improves the diagnostic sensitivity while eliminating the possibility of FP results.</P>
Nanoparticle additions for enhanced flux pinning in YBCO HTS films
N.M. Strickland,N.J. Long,E.F. Talantsev,P. Hoefakker,J.A. Xia,M.W. Rupich,W. Zhang,X. Li,T. Kodenkandath,Y. Huang 한국물리학회 2008 Current Applied Physics Vol.8 No.3,4
We describe a method for forming a nanoparticle dispersion in YBa₂Cu₃O7 superconducting lms grown by metal-organic depositionon RABiTS substrates. Two separate precursor modications have been shown by transmission electron microscopy to give nanopar-ticles of (Y,Dy)₂O₃ and BaZrO₃ with size distributions of 10-50 nm and 10-25 nm, respectively and that are well dispersed in the lm.These particles are eective ux pinning centers and enhance the transport critical current by up to 100% at 77 K for a magnetic eld of 1.5 T applied perpendicular to the tape. The processing required to grow these lms is not greatly altered from that of unmodied YBCO and can be readily transferred to continuous processing of long-length wires.
Zou, Jun,Fu, Donghui,Gong, Huihui,Qian, Wei,Xia, Wei,Pires, J. Chris,Li, RuiYuan,Long, Yan,Mason, Annaliese S.,Yang, Taex2010,Jin,Lim, Yong P.,Park, Beom S.,Meng, Jinling Blackwell Publishing Ltd 2011 The Plant journal Vol.68 No.2
<P><B>Summary</B></P><P>Interspecific hybridization is a significant evolutionary force as well as a powerful method for crop breeding. Partial substitution of the AA subgenome in <I>Brassica napus</I> (A<SUP>n</SUP>A<SUP>n</SUP>C<SUP>n</SUP>C<SUP>n</SUP>) with the <I>Brassica rapa</I> (A<SUP>r</SUP>A<SUP>r</SUP>) genome by two rounds of interspecific hybridization resulted in a new introgressed type of <I>B.?napus</I> (A<SUP>r</SUP>A<SUP>r</SUP>C<SUP>n</SUP>C<SUP>n</SUP>). In this study, we construct a population of recombinant inbred lines of the new introgressed type of <I>B.?napus</I>. Microsatellite, intron‐based and retrotransposon markers were used to characterize this experimental population with genetic mapping, genetic map comparison and specific marker cloning analysis. Yield‐related traits were also recorded for identification of quantitative trait loci (QTLs). A remarkable range of novel genomic alterations was observed in the population, including simple sequence repeat (SSR) mutations, chromosomal rearrangements and retrotransposon activations. Most of these changes occurred immediately after interspecific hybridization, in the early stages of genome stabilization and derivation of experimental lines. These novel genomic alterations affected yield‐related traits in the introgressed <I>B.?napus</I> to an even greater extent than the alleles alone that were introgressed from the A<SUP>r</SUP> subgenome of <I>B.?rapa</I>, suggesting that genomic changes induced by interspecific hybridization are highly significant in both genome evolution and crop improvement.</P>
Phan, The-Long,Ho, T.A.,Thang, P.D.,Tran, Q.T.,Thanh, T.D.,Phuc, N.X.,Phan, M.H.,Huy, B.T.,Yu, S.C. Elsevier 2014 JOURNAL OF ALLOYS AND COMPOUNDS Vol.615 No.-
<P><B>Abstract</B></P> <P>We have determined the values of critical exponents of two polycrystalline samples (Nd<SUB>1−</SUB> <I> <SUB>x</SUB> </I>Y<I> <SUB>x</SUB> </I>)<SUB>0.7</SUB>Sr<SUB>0.3</SUB>MnO<SUB>3</SUB> (<I>x </I>=0 and 0.07) from the magnetization data versus temperature and magnetic field, <I>M</I>(<I>H</I>, <I>T</I>), to learn about their magnetic and magnetocaloric (MC) properties. The results reveal the samples exhibiting the crossover of first-order and second-order phase transitions, where the exponent values <I>β </I>=0.271 and <I>γ </I>=0.922 for <I>x </I>=0, and <I>β </I>=0.234–0.236 and <I>γ </I>=1.044–1.063 for <I>x </I>=0.07 determined by using modified Arrott plots and static-scaling hypothesis are close to those expected for the tricritical mean-field theory (<I>β </I>=0.25 and <I>γ </I>=1.0). Particularly, the <I>T</I> <SUB>C</SUB> of <I>x </I>=0 and 0.07 can be any value in the temperature ranges of 240–255K and 170–278K, respectively, depending on the magnitude of applied magnetic field and determination techniques. Around the <I>T</I> <SUB>C</SUB>, studying the MC effect of the samples has revealed a large magnetic-entropy change (Δ<I>S</I> <SUB>m</SUB>) up to ∼8J/kgK for the applied field interval Δ<I>H </I>=50kOe, corresponding to refrigerant capacity values of 200–245J/kg. These phenomena are related to the crossover nature and the persisting of FM/anti-FM interactions even above the <I>T</I> <SUB>C</SUB>, as further confirmed by electron-spin-resonance data, Curie–Weiss law-based analyses, and an exponential parameter characteristic of magnetic order <I>n </I>=dLn|Δ<I>S</I> <SUB>m</SUB>|/dLn<I>H</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Tricritical point in Y-doped Nd<SUB>0.7</SUB>Sr<SUB>0.3</SUB>MnO<SUB>3</SUB> manganites. </LI> <LI> A large magnetic-entropy change. </LI> <LI> Magnetic inhomogeneity and phase separation. </LI> </UL> </P>
Spracklen, C. N.,Chen, P.,Kim, Y. J.,Wang, X.,Cai, H.,Li, S.,Long, J.,Wu, Y.,Wang, Y. X.,Takeuchi, F. IRL Press 2017 Human molecular genetics Vol.26 No.9
<P>Large-scale meta-analyses of genome-wide association studies (GWAS) have identified > 175 loci associated with fasting cholesterol levels, including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG). With differences in linkage disequilibrium (LD) structure and allele frequencies between ancestry groups, studies in additional large samples may detect new associations. We conducted staged GWAS meta-analyses in up to 69,414 East Asian individuals from 24 studies with participants from Japan, the Philippines, Korea, China, Singapore, and Taiwan. These meta-analyses identified (P < 5 x 10(-8)) three novel loci associated with HDL-C near CD163-APOBEC1 (P = 7.4 x 10(-9)), NCOA2 (P = 1.6 x 10(-8)), and NID2-PTGDR (P = 4.2 x 10(-8)), and one novel locus associated with TG near WDR11-FGFR2 (P = 2.7 x 10(-10)). Conditional analyses identified a second signal near CD163-APOBEC1. We then combined results from the East Asian meta-analysis with association results from up to 187,365 European individuals from the Global Lipids Genetics Consortium in a trans-ancestry meta-analysis. This analysis identified (log(10)Bayes Factor a 6.1) eight additional novel lipid loci. Among the twelve total loci identified, the index variants at eight loci have demonstrated at least nominal significance with other metabolic traits in prior studies, and two loci exhibited coincident eQTLs (P < 1 x 10(-5)) in subcutaneous adipose tissue for BPTF and PDGFC. Taken together, these analyses identified multiple novel lipid loci, providing new potential therapeutic targets.</P>
Long, D.X.,Xu, Y.,Kang, S.J.,Park, W.T.,Choi, E.Y.,Nah, Y.C.,Liu, C.,Noh, Y.Y. Elsevier Science 2015 ORGANIC ELECTRONICS Vol.17 No.-
We report on high-performance organic field-effect transistors (OFETs) with low-cost Mo source/drain electrode by incorporating solution-processed V<SUB>2</SUB>O<SUB>5</SUB> thin-film interlayers. The original V<SUB>2</SUB>O<SUB>5</SUB> powder of orange color is dissolved in NH<SUB>3</SUB> solvent (0.5wt%) and changed into a transparent solution after moderate heating. We are able to fabricate V<SUB>2</SUB>O<SUB>5</SUB> thin films (1-6nm) on Mo electrodes by simple spin-coating. Kelvin probe measurements reveal that the work function of the Mo electrodes progressively changed from 4.4 to 4.9eV. With the insertion of the V<SUB>2</SUB>O<SUB>5</SUB> layer, OFETs gained significantly improved p-type performance for both regioregular poly(3-hexylthiophene) (rr-P3HT) and ambipolar 3,6-Bis-(5bromo-thiophen-2-yl)-N,N-bis(2-octyl-1-dodecyl)-1,4-dioxo-pyrrolo[3,4-c]pyrrole (DPPT-TT) polymer semiconductors. OFETs on the basis of rr-P3HT or DPPT-TT with V<SUB>2</SUB>O<SUB>5</SUB>/Mo electrodes exhibit field-effect mobilities of 0.15 and 1.91cm<SUP>2</SUP>/Vs, respectively, highly improved from 0.05 and 1.2cm<SUP>2</SUP>/Vs in case of OFETs with bare Mo. This performance is already comparable with that of OFETs employing much more expensive Au electrodes. After investigating the contact resistance, we propose that the improvement relies on balancing two factors: modulating the injection barrier by tuning the work function, and manipulating the tunnel distance by controlling the film thickness.