http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Expression of the Dipeptidyl Peptidase-4 Gene in Porcine Cumulus Cells and Parthenogenetic Embryos
Ji-Hyun Lim,Hak-Jae Chung,Kyong-Woon Kim,Sun-Young Back,Won-Gil Lee,You-Mi Cho,Soon-Hyuk Kim,Narae Son,Jin-Ki Park,Mi-Ryung Park 한국동물생명공학회(구 한국동물번식학회) 2013 발생공학 국제심포지엄 및 학술대회 Vol.2013 No.1
DPP-4 (Dipeptidyl peptidase IV/CD26) is a 110 kDa cell surface glycoprotein that belongs to the serine protease family and is expressed on a variety of tissues, including T lymphocytes, endothelial cells, and epithelial cells. It is composed of a short cytoplasmic domain, a transmembrane region, and an extracellular domain with dipeptidyl peptidase IV activity. The present study aimed to investigate the localization of DPP-4 in porcine ovary and parthenogenetic embryos. DPP-4 was detected not only in immatured and matured COCs but also until blastocysts stage by immunocytochemical analysis. Especially, the cumulus cells were higher expressed than that of the oocytes. We attempted DPP-4 downregulation of porcine embryos by siRNA, and evaluated DPP-4 suppression of developmental competencies. Although the porcine embryos injected with DPP-4 siRNA were able to develop to the early stage, these embryos were decreased to form blastocysts. Our results indicated that DPP-4 is an important factor for the regulation of development of porcine embryos.
( Ji Hyun Lee ),( Jin Huh ),( Duk Kyung Kim ),( Jea Ryoung Gil ),( Sung Won Min ),( Sun Sook Han ) 대한마취과학회 2010 Korean Journal of Anesthesiology Vol.59 No.6
Background: Laparoscopic cholecystectomy (LC) has traditionally been performed under general anesthesia, however, owing in part to the advancement of surgical and anesthetic techniques, many laparoscopic cholecystectomies have been successfully performed under the spinal anesthetic technique. We hoped to determine the feasibility of segmental epidural anesthesia for LC. Methods: Twelve American Society of Anesthesiologists class I or II patients received an epidural block for LC. The level of epidural block and the satisfaction score of patients and the surgeon were checked to evaluate the efficacy of epidural block for LC. Results: LC was performed successfully under epidural block, with the exception of 1 patient who required a conversion to general anesthesia owing to severe referred pain. There were no special postoperative complications, with the exception of one case of urinary retention. Conclusions: Epidural anesthesia might be applicable for LC. However, the incidence of intraoperative referred shoulder pain is high, and so careful patient recruitment and management of shoulder pain should be considered.
Asymmetric distribution of mitochondria in the vegetal marginal zone of the ascidian embryo
Ji Won Lee,Gil Jung Kim 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8
The mechanisms by which embryo exactly distributes mitochondria into the blastomeres during embryogenesis are one of the important issues in developmental biology. Although the mechanisms has been thought to be important for the proper embryonic development, our understanding has remained limited. In the present study, the distribution of mitochondria was examined in embryos of the ascidian, Halocynthia roretzi, by immunohistochemical staining with three-types of the mitochondria-specific antibodies and vital staining of mitochondria with a fluorescent probe, DiOC2(3). Results of the immunohistochemical staining coincided with that of vital staining, which is able to detect the distribution of mitochondria in cytoplasm of the embryo. Mitochondria was mainly segregated into the B4.1 posterior-vegetal blastomeres at the 8-cell stage. During the next stages, mitochondria was preferentially partitioned into cells of the B-line muscle and the A-line nerve cord precursor compared with each sister cell, endoderm in the 5th cleavage stage, and mesenchyme and notochord in the 6th cleavage stage. However, the mitochondria-rich cytoplasm is divided equally among the blastomeres of the animal hemisphere between the 8-cell and the 64-cell stages. When B6.2 blastomeres were isolated at the early 32-cell stage embryo and cultured in seawater, until control embryos reached the 64-cell stage, pattern of mitochondria distribution was similar to results of the coisolated B7.3 and B7.4 blastomeres from the 64-cell stage embryos. Therefore, it is likely that mitochondria are asymmetrically segregated into the marginal cells in the vegetal hemisphere of the ascidian embryo without cell-cell interaction.
Bioassay Associated with Tau-fluvalinate Resistance in the Mite, Varroa destructor, from Korea
Won Tae Kim,Myung Ryul Lee,Ji Young Choi,Jong Gill Kim,Young Cheol Choi,Kwan Ho Park,Gil Sang Jeong,Dae-Weon Lee,Young Ho Koh 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Varroa destructor is an ecto-parasite mite and worldwide pest of the honey bee Apis mellifera L. The pyrethroid tau-fluvalinate (Apistan), an acaricide that is tolerated by honey bees, has been used for varroa mite control since the mid 1980s. Even though various resistances to tau-fluvalinate in varroa mites have been reported from Europe, Israel, and USA, the nature of tau-fluvalinate resistance in varroa mites in Korea has never been investigated. To investigate and understand tau-fluvalinate resistance in varroa mites in Korea, we conducted bioassay in several apiaries located different regions in Korea. To understand the molecular mechanisms underlying the difference of tau-fluvalinate resistances in varroa mites, partial genomic DNA fragments of a voltage-sensitive sodium channel gene from varroa mites were cloned and sequenced, since tau-fluvalinate is known to act on the sodium channels directly. Two novel mutations in sodium channels of varroa mites were present in eight apiaries. Two mutations might be a geographical polymorphism of sodium channel of varroa mites in Korea.
( Gil-sun Hong ),( Jae Ho Byun ),( Heon-ju Kwon ),( Ji Yeon Kim ),( So Yeon Kim ),( Hyung Jin Won ),( Kyung Won Kim ),( Young Moon Shin ),( Pyo Nyun Kim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: To identify imaging characteristics that differentiate small (£2 cm) HCCs from small (£2 cm) benign nodules in cirrhotic liver on gadoxetic acidenhanced and diffusion-weighted (DW) magnetic resonance (MR) images. Methods: On gadoxetic acid-enhanced and DW MR images, we analysed signal intensity of 222 small HCCs and 61 benign nodules (diameter, 0.5-2 cm) at each sequence and rim enhancement during portal or equilibrium phases. Univariate and multivariate logistic regression analyses identified predictors of HCC. Combinations of significant MR findings in multivariate analysis were compared with American Association for the Study of Liver Disease (AASLD) practice guidelines. Results: In multivariate analysis, arterial enhancement (adjusted odds ratio [aOR], 8.6), T2 hyperintensity (aOR,5.8), and hyperintensity on DW images (aOR, 3.8) were significant for differentiating small HCCs from benign nodules (p £ 0.004). When two or all three findings were applied as diagnostic criteria for differentiating small HCCs from benign nodules, sensitivity and accuracy were significantly higher compared with AASLD practice guidelines (91% vs. 78% and 89% vs. 81%, respectively;each P<0.0001). Conclusions: On gadoxetic acid-enhanced MR imaging, arterial enhancement and hyperintensity on T2-weighted and DW MR images are helpful for differentiating small HCCs from benign nodules in liver cirrhosis.
Design and Architecture of Low-Latency High-Speed Turbo Decoders
Ji Won Jung,Duk Gun Choi,Jin Hee Jeong,Ki Man Kim,Eun-A Choi,Deock-Gil Oh,In Ki Lee 한국전자통신연구원 2005 ETRI Journal Vol.27 No.5
In this paper, we propose and present implementation results of a high-speed turbo decoding algorithm. The latency caused by (de)interleaving and iterative decoding in a conventional maximum a posteriori turbo decoder can be dramatically reduced with the proposed design. The source of the latency reduction is from the combination of the radix-4, center to top, parallel decoding, and early-stop algorithms. This reduced latency enables the use of the turbo decoder as a forward error correction scheme in real-time wireless communication services. The proposed scheme results in a slight degradation in bit error rate performance for large block sizes because the effective interleaver size in a radix-4 implementation is reduced to half, relative to the conventional method. To prove the latency reduction, we implemented the proposed scheme on a fieldprogrammable gate array and compared its decoding speed with that of a conventional decoder. The results show an improvement of at least five fold for a single iteration of turbo decoding.
Bacterial infection monitoring in the early period after liver transplantation
Ji Soo Lee,Seung Hwan Lee,Kyeong Sik Kim,Eun Mi Gil,Gyu-Seoung Choi,Jong Man Kim,Kyong Ran Peck,Choon Hyuck David Kwon,Jae-Won Joh,Suk-Koo Lee 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.94 No.3
Purpose: Infection remains the main cause of morbidity and mortality in liver transplantation (LT) recipients; however infection is notoriously difficult to diagnose because its usual signs and symptoms of infection may be masked or absent. This study comprises an analysis of bacterial infections in the early period after LT. Methods: This is a study of 129 adults who underwent LT from January 2013 to December 2013, and it includes patients who were followed daily from the day of transplantation to 1-week posttransplantation using bacteriological cultures of blood, urine, sputum, and drained ascites. Results: The following factors were significantly different between the positive and negative culture groups: living donor LT vs. deceased donor LT (odds ratio [OR], 3.269; P = 0.003), model for end-stage liver disease score (OR, 4.364; P < 0.001), and Child-Pugh classification (P = 0.007). Neither positive culture nor negative culture was associated with infection within 4 weeks of surgery (P = 0.03), and most events were due to surgical complications (75%). Conclusion: Since the full effect of immunosuppression is not yet present during the first month after LT, we suggest that the number of bacterial culture test could be reduced such that they are performed every other day depending on patient’s situation