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Bin Dong,Yanjun Lin,Zhiwei Su,Chunlong Sun,Jun Wang,Shijun Fu,Wen Du,Tao Wu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.7
Foodborne pathogens causing food poisoning and infections are detrimental to human health, and the abuse of antibiotics induced severe antibiotic resistance in past decades. Thus, it is urgent to develop new antimicrobial agents. In the current study, human β-defensin 130 (hBD130), which is an antimicrobial peptide identified in human macrophages in 2017, was initially produced in Pichia pastoris. The purified hBD130 demonstrated broad bactericidal spectrum against foodborne pathogens through a membrane disruption, with concentrations ranging from 10 to 45 μg/mL. Moreover, hBD130 showed a low hemolytic effect and nearly no cytotoxicity to mammalian cells with a dosage of 400 μg/mL. In addition, the secretion amounts and mRNA levels of NO, IL-6, IL-1β, and TNF-α in LPS-induced mouse macrophage were significantly decreased with 1 mg/mL of hBD130. Taken together, these results showed that hBD130 is a promising antimicrobial agent to treat foodborne bacterial infections and inflammation.
Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3
Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.
Bin Dong,Yanjun Lin,Jun Wang,Wen Du,Chunlong Sun,Tao Wu,Shijun Fu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.5
Pork and its products are preferably contaminated by bacteria; thus, it is essential to develop low-cost, high-efficiency and biologically safe preservatives to prevent the growth of bacteria during storage. In the current study, grass carp β-defensin 1 (gcDefb1) was produced and purified from Pichia pastoris through the heterologous expression method. The in vitro antimicrobial assay demonstrated that yeast-derived gcDefb1 possesses a broad antibacterial spectrum, including both Gram-positive and -negative bacteria, and the MIC values against Escherichia coli ATCC 25,922 were as low as 30 μg/mL and showed no cytotoxicity or hemolytic activity. The bactericidal rate of gcDefb1 was less than 60 min by disrupting the cell membranes, and it inhibited the formation of bacterial biofilms. Moreover, gcDefb1 was used as a biopreservative for pork storage, indicating that the physicochemical and sensory qualities were improved. This study provides an efficient method to prepare and utilize gcDefb1 as a novel biopreservative.
Bin Yang Wu,Qiang Zhan,Xiao Yang Yu,Wen Yu Gu,Min Zhang,Wanhua Su 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.4
In-cylinder charge density at top dead center is an important parameter of diesel engines and is influenced by intake pressure, intake temperature, and compression ratio. The effects of charge density on fuel spray, combustion process, and emissions were investigated by using a constant volume bomb and a heavy-duty diesel engine. Spray development resistance increased with the increase of the charge density in the constant volume bomb. It was found that short spray penetration was accompanied by a large spray cone angle in the former stage with high charge density. However, the equivalence ratio was lowered and the degree of homogeneity of the mixture was increased in the later stage owing to the rapid interaction of fuel and gas at a high mixing rate. Combining the first law of thermodynamics and the second law of thermodynamics for analysis, as the charge density increased, the gross indicated thermal efficiency (ITEg) was improved. However, pumping loss had to be considered with higher charge density. Under this condition, the brake thermal efficiency (BTE) trend was increased initially and decreased subsequently. Under high-load operation (1200 r/min BMEP, 2.0 MPa), the minimum charge density value of 44.8 kg/m3 was found to be reasonable. This charge density was suitable for combustion and brought about minimum exhaust energy and trade-off emissions. Moreover, by analyzing two operation conditions in terms of the maximum BTE with the Miller and the conventional cycles, compression temperature and combustion temperature were reduced in the Miller cycle with the charge density 44.8 kg/m3. A high C p/C v could improve the cylinder exergy/power conversion process by its positive effect of increasing the specific heat ratio. Owing to the interaction between a high C p/C v and exergy loss to heat transfer, the condition with the minimal charge density could produce more piston work.
Wu, Jiong,Zhou, Yan,Zhang, Chun-Yan,Song, Bin-Bin,Wang, Bei-Li,Pan, Bai-Shen,Lou, Wen-Hui,Guo, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Background: The aim of our study was to establish COLD-PCR combined with an unlabeled-probe HRM approach for detecting KRAS codon 12 and 13 mutations in plasma-circulating DNA of pancreatic adenocarcinoma (PA) cases as a novel and effective diagnostic technique. Materials and Methods: We tested the sensitivity and specificity of this approach with dilutions of known mutated cell lines. We screened 36 plasma-circulating DNA samples, 24 from the disease control group and 25 of a healthy group, to be subsequently sequenced to confirm mutations. Simultaneously, we tested the specimens using conventional PCR followed by HRM and then used target-DNA cloning and sequencing for verification. The ROC and respective AUC were calculated for KRAS mutations and/or serum CA 19-9. Results: It was found that the sensitivity of Sanger reached 0.5% with COLD-PCR, whereas that obtained after conventional PCR did 20%; that of COLD-PCR based on unlabeled-probe HRM, 0.1%. KRAS mutations were identified in 26 of 36 PA cases (72.2%), while none were detected in the disease control and/or healthy group. KRAS mutations were identified both in 26 PA tissues and plasma samples. The AUC of COLD-PCR based unlabeled probe HRM turned out to be 0.861, which when combined with CA 19-9 increased to 0.934. Conclusions: It was concluded that COLD-PCR with unlabeled-probe HRM can be a sensitive and accurate screening technique to detect KRAS codon 12 and 13 mutations in plasma-circulating DNA for diagnosing and treating PA.
Research on the Correlation between Wavy Modeling of Garment and Fabric Properties
( Wen Bin Zhang ),( Wu Tuo ),( Jing Ru Liu ) 한국패션비즈니스학회 2004 패션 비즈니스 Vol.8 No.6
On the basis of garment spatial configuration technology, the correlation of fabric properties and garment modeling was studied in this paper. With abundant experimental data, practical modeling and the judgment of fabric properties and physical shape, we analyzed the change rules that restricting all kinds of parameters of fabric properties and garment modeling, and discussed the relation of wavy modeling and all correlated factors of fabric properties. The result showed the basis of choosing fabric for different style garment, which would help designers choosing fabric for modeling design or structure design.
Wu, Xiao-Yuan,Lu, Can-Zhong,Xia, Chang-Kun,Chen, Shu-Mei,Liu, Jiu-Hui,Chen, Li-Juan,Yang, Wen-Bin Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.8
Four isostructural amino-acid-based polyoxomolybdates, {$M(H_2O)_3(pro)Mo_4O_{13}$}$_2{\cdot}2H_2O$ (pro = proline, M = Co (1), Ni (2), Cu (3), Zn (4)), have been synthesized and structurally characterized by single crystal X-ray diffraction, elemental analysis, IR spectrum, TG analysis. The structures of 1-4 are layered networks built up from {$Mo_8O_{26}(pro)_2$}$^{4-}$ units and {$M(H_2O)_3O_3$} octahedra, the uncoordinated water molecules occupying the interlayer regions.
Novel Phage Display-Derived H5N1-Specific scFvs with Potential Use in Rapid Avian Flu Diagnosis
( Jie Wu ),( Xian Qiao Zeng ),( Hong Bin Zhang ),( Han Zhong Ni ),( Lei Pei ),( Li Rong Zou ),( Li Jun Liang ),( Xin Zhang ),( Jin Yan Lin ),( Chang Wen Ke ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5
The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.
A Comparative Study of Camouflage Printing Color Matching Based on Monitor and Paper Card
Yu-wen Wang,Qing-zhu Yi,Yi Ding,Guang-xin Wu,Jing-bin Zhang,Ni Wang 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4
To improve the accuracy of color reproduction for camouflage printing, a new color matching method, namedmonitor-paper-fabric, was proposed by importing color management into textiles, which matched the printing pastesaccording to the color of the paper card printed by the ink-jet printer after color management. Not like the traditional colormatching method, it matched the printing pastes according to the color from computer monitor after color correction. Twocolor matching methods were analyzed by comparing the color difference. It was found that the “monitor-paper-fabric” colormatching method could be considered as a convenient and feasible color matching method. Most color differences betweenthe monitor and the fabric in the camouflage pattern were reduced to lower than 4, except for color blocks 5 and 6. Inaddition, the color differences of six color blocks between the paper and the fabric were all less than 3.5, and were lower thanthose between the monitor and the fabric. The color consistency between the paper and the fabric was better than thatbetween the monitor and the fabric.
Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China
Yuan Fang,Bin Li,Fang Wang,Baoping Liu,Zhiyi Wu,Ting Su,Wen Qiu,Guanlin Xie 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4
An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.