Risk Factors for Early Recurrence of HBV-related Hepatocellular Carcinoma Meeting Milan Criteria after Curative Resection

Zhu, Wen-Jiang,Huang, Chu-Ying,Li, Chuan,Peng, Wei,Wen, Tian-Fu,Yan, Lv-Nan,Li, Bo,Wang, Wen-Tao,Xu, Ming-Qing,Yang, Jia-Yin,Jiang, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: The prognosis of patients with hepatocellular carcinoma (HCC) after curative resection varies greatly. Few studies had investigated the risk factors for early recurrence (recurrence-free time ${\leq}$ 1 year) of hepatitis B virus (HBV)-related HCCs meeting Milan criteria. Methods: A retrospective analysis was performed on the 224 patients with HCC meeting Milan criteria who underwent curative liver resection in our center between February 2007 and March 2012. The overall survival (OS) rate, recurrence-free survival (RFS) rate and risk factors for early recurrence were analyzed. Results: After a median follow-up of 33.3 months, HCC reoccurred in 105 of 224 patients and 32 died during the period. The 1-, 3- and 5-year OS rates were 97.3%, 81.6% and 75.6% respectively, and the 1-, 3- and 5-year RFS rates were 73.2%, 53.7% and 41.6%. Cox regression showed alpha-fetoprotein (AFP) > 800 ng/ml (HR 2.538, 95% CI 1.464-4.401, P=0.001), multiple tumors (HR 2.286, 95% CI 1.123-4.246, P=0.009) and microvascular invasion (HR 2.518, 95% CI 1.475-4.298, P=0.001) to be associated with early recurrence (recurrence-free time ${\leq}$ 1-year) of HCC meeting Milan criteria. Conclusions: AFP > 800 ng/ml, multiple tumors and microvascular invasion are independent risk factors affecting early postoperative recurrence of HCC. In addition resection appears capable of replacing liver transplantation in some situations with safety and a better outcome.

Mitochondrial NDUFA4L2 attenuates the apoptosis of nucleus pulposus cells induced by oxidative stress via the inhibition of mitophagy

Wen-Ning Xu,Huo-Liang Zheng,Run-Ze Yang,Tao Liu,Wei Yu,Xin-Feng Zheng,Bo Li,Sheng-Dan Jiang,Lei-Sheng Jiang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The main pathological mechanism of intervertebral disc degeneration (IVDD) is the programmed apoptosis of nucleus pulposus (NP) cells. Oxidative stress is a significant cause of IVDD. Whether mitophagy is induced by strong oxidative stress in IVDD remains to be determined. This study aimed to investigate the relationship between oxidative stress and mitophagy and to better understand the mechanism of IVDD in vivo and in vitro. To this end, we obtained primary NP cells from the human NP and subsequently exposed them to TBHP. We observed that oxidative stress induced mitophagy to cause apoptosis in NP cells, and we suppressed mitophagy and found that NP cells were protected against apoptosis. Interestingly, TBHP resulted in mitophagy through the inhibition of the HIF-1α/NDUFA4L2 pathway. Therefore, the upregulation of mitochondrial NDUFA4L2 restricted mitophagy induced by oxidative stress. Furthermore, the expression levels of HIF-1α and NDUFA4L2 were decreased in human IVDD. In conclusion, these results demonstrated that the upregulation of NDUFA4L2 ameliorated the apoptosis of NP cells by repressing excessive mitophagy, which ultimately alleviated IVDD. These findings show for the first time that NDUFA4L2 and mitophagy may be potential therapeutic targets for IVDD.

Head-on Collision Between Two Envelope Solitary Waves in a Granular Medium

Wen-Qing Du,Jian-An Sun,Juan-Fang Han,Wen-Shan Duan,Yang-Yang Yang,Xin Jiang 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.75 No.11

We investigated the head-on collision between two envelope solitary waves. Head-on collisions between two envelope solitary waves are first discussed in one-dimension (1D) granular chains. The interesting result is that no phase shift or phase delay detected after the head-on collision between two envelope solitary waves. The maximum amplitude during the head-on collision between two envelope solitary waves is also found to be less than the sum of the amplitudes of the two envelope solitary waves, but is larger than the amplitude of the either of the envelope solitary waves.

Design of Micro Biopsy Device for Wireless Autonomous Endoscope

Wen-Wen Chen,Guo-Zheng Yan,Hua Liu,Ping-Ping Jiang,Zhi-Wu Wang 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

Well-established technology that provides assistance from outside the patient may soon give way to alternative approaches that bythe wireless robotic mechanisms inside the patient through natural orifices. For this study, two kinds of micro biopsy mechanisms weredeveloped for capsule endoscope which could traverse the gut environment automatically. This mobility allows the biopsy device tomove to the area of interest to sample specific tissues. Based on the medical and mechanism considerations, we concerned on designprecise biopsy jaw with sufficient cutting force, and the biopsy process should be safe, repeatable and visible. The lead-screw linkagesystem that actuated jaw of biopsy allowing for large force production. Using one micro-motor, the biopsy process was carried outquickly by three motions: stretching to sampling point; bite and cutting off; withdrawing into capsule body with the sample. With thecontrol and vision system, the biopsy process could compete automatically. The robot was capable of traversing in the gut environmentwirelessly and had been successfully used to biopsy gut tissue in vitro. In addition, experimental analysis of the biopsy mechanismshowed good results towards more elaborate tissue manipulation in the future.

High Speed Abrasive Electrical Discharge Machining of Particulate Reinforced Metal Matrix Composites

Jiang-Wen Liu,Yong-Zhong Wu,Tai-Man Yue 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

A high speed abrasive electrical discharge machining (AEDM-HS) process has been developed to improve the performance and save the machining energy of the conventional EDM process in machining particulate reinforced metal matrix composites (MMCs). The AEDM-HS process functions under a combined action of spark erosion and direct mechanical grinding. A set of experiments was conducted and the results showed that the material removal rate (MRR) of AEDM-HS was much higher than that of EDM under the experimental conditions of this study. Moreover, the surface roughness value (Ra) measured for the AEDM-HS specimen was about six times smaller than that of the specimen machined without high speed abrasive action (i.e., EDM alone). The material removal mechanism of this novel process has been analyzed by means of single-pulse experimentation. And the relative importance of the various processing parameters on MRR was established using orthogonal analysis. The results showed that the MRR is influenced by the machining factors in the order of duty cycle > current > pulse duration. This study showed that the AEDM-HS process is superior to the EDM process for machining particulate reinforced MMCs, where a higher machining efficiency and a better surface quality can be obtained.

Anti-inflammatory effect of SR823, standardized fraction of Salvia miltiorrhiza in RAW264.7 macrophages via induction of heme oxygenage-1 expression

JIANG Wen-Yi,CHE Xian-Hua,PARAJULI Daya Ram,SOHN Dong Hwan,LEE Sung Hee 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1

Modification of a Smoking Motivation Questionnaire for Chinese Medical Students

Jiang, Chao,Sun, Wen-Jie,Wan, Yan-Chun,Wei, Ming-Wei,Mu, Yong-Ping,Tarver, Siobhan L.,Gao, Yong-Qing,Hu, Tian,Xu, Chao,Gordon, James,Feng, Cindy Xin,Wen, Yu-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Introduction: Smoking prevalence among the medical students is high in China. Therefore, understanding the smoking motivations of medical students is crucial for smoking control, but currently there are no scales questionnaires customized for probing the smoking motivations of medical students. This aim of study was to test and modify a questionnaire for investigating smoking motivations among medical students. Methods: A cross-sectional survey was conducted among 1,125 medical students at Xuzhou Medical College in China in 2012.The model fit and validity was assessed by confirmatory factor analysis (CFA) and the reliability was tested by single-item reliability, composite reliability, and item-total correlation. Results: The prevalence of smoking was 9.84 % among study population. In the modified scales, the global fit indices identified a CFI value of 0.96, TLI was 0.96, and the RMSEA was 0.063. CFA supported the two dimensional structure of the instrument. The average variance extracted ranged from 0.45 to 0.62. All single-item reliability scores were greater than 0.20, and the composite reliability ranged from 0.74 to 0.91. Conclusion: Modified scales could be the preliminary instrument used in evaluating the smoking motivations of medical students. However, it should be further assessed using other forms and methods of validity and reliability, additional motivations of smoking, and the survey of other medical colleges in China.

Temperature control for a 5kW water-cooled PEMFC using fuzzy control

Wen-Jiang Zou,Young-Bae Kim 한국에너지학회 2018 한국에너지공학회 학술발표회 Vol.2018 No.11

Protein corona on magnetite nanoparticles and internalization of nanoparticle–protein complexes into healthy and cancer cells

Wen Jiang,Kuilin Lai,Yao Wu,Zhongwei Gu 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.1

Superparamagnetic magnetite nanoparticles(MNPs) of different surface properties are incubated incomplicated living fluid, including fetal bovine serumsolution, cell complete culture medium and cell culturesystem with/without serum, to investigate the alteration ofprotein corona and its impact on cell internalization. TheMNPs prepared by co-precipitation method are functionalizedwith L-Lysine (Lys), Glucosamic acid (GA) to obtainamine, carboxyl and hydroxyl groups, separately. All theparticles adsorb serum proteins to form MNPs–proteincomplexes with the surface charge changing into negative. 1D SDS/PAGE gel images analysis indicates that thecomposition and content of hard protein corona on thesurface of NPs are related to their functional groups andagglomeration, and the total amount of protein in themedium. In cell culture system, particles not only adsorbserum proteins, but also associate with cytosolic proteinsarising from HepG2 and L02 cells. GA modified MNPs(MNPs-GA) exhibit bovine serum albumin anti-adsorptioncapability because of the terminal hydroxyl and carboxylgroups. MNPs-GA also shows the highest cellular uptakeand label efficiency compared with uncoated MNPs andLys modified MNPs, due to larger aggregates formationand specific protein corona composition, rather than commonlyapproved electrostatic interaction between particles and cells. For the first time, our results provide visualizedreports on previously neglected, but indispensable proteincorona of the MNPs after interaction with both healthy andcancer cells, suggesting that cytosolic protein corona fromcells and aggregation of particles are important factorsneeded to be account for on studying the nano–biointerface.

PF2405, standardized fraction of Scutellaria baicalensis, ameliorates colitis in vitro and in vivo

Wen-Yi Jiang,서검석,김윤철,손동환,이성희 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Standardized extraction procedures for herb areas important as their authentication to maintain theirquality and ensure their safe use. We had prepared astandardized and purified Scutellaria baicalensis Georgiextract, PF2405, which was enriched with three majorcomponents, baicalein, oroxylin A and wogonin. In thepresent study, we investigated the potential anti-inflammatoryeffects of PF2405 in vitro and in two differentexperimental animal models of inflammatory bowel disease. Effect of PF2405 studied in tumor necrosis factor(TNF)-a-induced HT-29 cells in vitro. In vivo experimentalcolitis models were induced by administration oftrinitrobenzene sulfonic acid (TNBS) or dextran sulfatesodium (DSS). PF2405 (50 lg/ml) decreased TNF-ainducedcyclooxygenase (COX)-2 expressions throughinhibition of phosphorylation of c-Jun N-terminal kinasesand p38 mitogen-activated protein kinase in HT-29 cells. Combination of baicalein (20 lg/ml), oroxylin A (8 lg/ml),and wogonin (2 lg/ml) markedly inhibits TNF-a-inducedCOX-2 expression when compared with individual components. PF2405 (25 mg/kg b.w.) treatment significantlyreduced histopathological severity; suppressed expression ofCOX-2, TNF-a, and interleukin-1b in TNBS-induced mice. Moreover, PF2405 (25 mg/kg b.w.) has both potent preventiveand therapeutic activities in DSS-induced colitis. Collectively, PF2405 shows prominent anti-inflammatoryeffect that can be used as a new therapeutic approach forintestinal inflammatory disorders.