다동온실의 복합환경 제어시스템을 위한 소프트웨어 개발

이대원,김채웅 성균관대학교 생명공학연구소 1999 生命工學硏究 Vol.5 No.1

A hybrid environmental control system was designed and built in the multi-greenhouses. After the hardware system was developed, the software was designed and constructed for this project. A software was adequate for reading data from many sensors and operating side windows, top windows, curtains and fans in the two greenhouses. Separate programs for control of the hardware, written Visual basic, Turbo-C and Assembler computer language, were developed to make the hybrid environmental control system operate. The program was a menu driven program within Windows environment. The initial menu was easily implemented by pressing a keypad key(KPK) on the keyboard or a mouse. Continued manipulation of the KPK or a mouse enable the user to reach easily any of the sub modes in the many modes. This software proved to be a reliable program for the hybrid environmental control system in the two greenhouse.

골절단을 이용한 급속 치아이동 후 치수 및 치주조직 변화

강경화,김은철,이선경,임채웅,마쓰다 기꾸,태기출,김상철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.2

본 연구는 골절단을 이용한 치아-치조골 분절의 급속 치아이동 후 치수, 치주인대 및 치조골의 변화를 유성견에서 평가하고자 하였다. 하악 제4소구치의 근심, 원심, 치근단 부위에서 피질골을 절단하여 치아-골 분절을 형성하고 하악 제3소구치를 발거하면서 협측, 설측 부위의 피질골을 삭제하였다. 1주 휴지기 부여에 따라 휴지기군과 비휴지기군으로 나누어 치아의존형 견인장치로 6일 동안 견인하고 강화기를 거친 후 0주, 1주, 2주, 4주, 6주, 8주에 희생시켜 치수, 치주인대 및 치조골의 조직 변화를 임상적, 방사선적, 조직학적 및 면역화학적으로 평가하여 다음과 같은 결과를 얻었다. 1. 치아이동 양과 강화기 동안의 조직 치유 양상에서 휴지기 유무에 따른 차이는 없었다. 2. 견인측에서 골형성은 강화기 8주까지 계속되었는데 강화기 1-2주에 가장 활발하였으며 6-8주간의 변화는 적었다. 3. 비휴지기군의 강화기 1주에서 치조골 흡수 및 파골세포 출현, 염증세포 침윤이 가장 많았으며, 특징적으로 파상아세포가 압박측의 치주인대와 치수 내에 나타났다. 4. TGF-β는 치조골의 골기질 및 골모세포, 파골세포, 치수 내 파상아세포에서 강양성 발현을 보인 반면 치수, 백악모세포, 무세포성 백악질에서 경미한 양성 발현을 나타내는 부위 특이성이 있었다. 5. TGF-β는 견인측 치주인대의 치조골에 인접한 혈관 및 치주인대세포, 골모세포에서 강화기 초기 1-2주에 주로 발현되었으며 6주 이후에는 발현이 크게 감소하였다. The movement of tooth-bone segments by osteotomy can simultaneously shift tooth and surrounding alveolar bone in a relatively short period. The purpose of this study was to evaluate the tissue changes in pulp, periodontal ligament, and alveolar bone in rapid tooth bone movement with osteotomy. The mandibular 3rd premolar of a dog was extracted and cortical bones of the buccal and lingual area were eliminated, and then cortical bones around the mesial and distal area of root, and below the root apex of the mandibular 4th premolar were osteotomized. After a one week latency period, a tooth-borne distraction device was activated for 6 days. And pulp, periodontal ligament and alveolar bone were evaluated clinically, radiologically, histologically and immunohistochemically at 0, 1, 2, 4, 6, 8 weeks of the consolidation period and conclusions were reached as follows. 1. Latency period didn't affect total amount of tooth movement and healing process of tissue during consolidation period. 2. Bone formation continued through 8 weeks of consolidation in distracted side, with a high peak at 1-2 weeks, and the lowest at 6-8 weeks of consolidation. 3. At 1 week of consolidation, alveolar bone resorption, osteoclast appearance and inflammatory cell infiltration were the most active, and dentinoclasts characteristically appeared on the pulp and pressure side of the periodontal ligament. 4. The expression of TGF-β was area-specific, as it was strong-positive at bone matrix, osteoblast, osteoclast of alveolar bone, and dentinoclast inside pulp, but weak in pulp, cementoblast and acellular cementum. 5. The expression of TGF-β was generally observed at the initial 1-2 weeks of consolidation at vessels, periodontal ligament cells, and osteoblast near alveolar bone on the distraction side of the periodontal ligament, and was significantly decreased after 6 weeks of consolidation.

Mossbauer 분광법에 의한 Fe₂SiO₄의 연구

이성호,김웅태,채광표,정덕조 建國大學校基礎科學硏究所 1988 理學論集 Vol.13 No.-

Fe₂SiO₄에 관한 X-선 회절실험과 Mossbauer 분광실험을 하였다. X-선 회절실험 결과 본 시료는 olivine group에 속하는 fayalite로서 orthorhombic 구조(??)임을 알 수 있었다. Mossbauer 분광실험은 60K-834K의 온도구간에서 행하여 졌으며, Mossbaure 스펙트럼을 분석한 결과 철은 high spin 2+ 상태임을 알 수 있었고, X-선 회절실험 결과와는 다르게 철이 산소 이온들과 함께 일그러진 octahedron을 형성하고 있음을 알 수 있었다. The Fe₂SiO₄ has been studied by means of X-ray diffractometry and Mossbauer spectroscopy. The analysis of X-ray diffraction pattern shows that Fe₂SiO₄is fayalite of orthorhombic structure(??). The Mossbauer spectra which have been taken between 60K and 834K show that the valence state of iron is ferrous and high spin state and that the iron ions occupy sites of distorted octahedron whereas the X-ray analysis shows it is at a site of regular one.

Investigation on properties of electron-beam treated Mo back contact layer prepared by DC sputtering method.

Kim, Chae-Woong,Jeong, Chaehwan American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.8

<P>Molybdenum (Mo) has been used as back contact layer in chalcopyrite solar cells family (CulnSe2 and its alloys) because it showed the excellence of electrical properties such as low resistivity at the Mo interference. Generally, there are strong corrleations between working pressure and properites such as adhesion and conductivity during deposition of Mo layer. Electrical properites might be inversely proportional to adehsion between Mo layer and glass substrate. Several methods have been executed for improvement of electrical properties and ahesion. In this study, DC sputtered Mo back contact layers on sodalime glass were prepared with working pressure range of 1-10 mTorr, and then all samples were treated by rounded exposure of electron beam at DC power of 3 kV for 5 minutes. After electron-beam treatement, all samples showed the lower resisitivity and sheet resisitance than as-deposited Mo layers. As working pressure decreased, grain size of e-beam treated samples gradually increased up to 30.7 nm. E-beam treated Mo layer might be a good candidate as back contact layer of tandem structured CIS families thin film solar cells.</P>

Intracellular cleavage of osteopontin by caspase-8 modulates hypoxia/reoxygenation cell death through p53.

Kim, Hyo-Jin,Lee, Ho-June,Jun, Joon-Il,Oh, Yumin,Choi, Seon-Guk,Kim, Hyunjoo,Chung, Chul-Woong,Kim, In-Ki,Park, Il-Sun,Chae, Han-Jung,Kim, Hyung-Ryong,Jung, Yong-Keun National Academy of Sciences 2009 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.106 No.36

<P>Osteopontin (OPN) is highly expressed in cancer patients and plays important roles in many stages of tumor progression, such as anti-apoptosis, proliferation, and metastasis. From functional screening of human cDNA library, we isolated OPN as a caspase-8 substrate that regulates cell death during hypoxia/reoxygenation (Hyp/RO). In vitro cleavage assays demonstrate that OPN is cleaved at Asp-135 and Asp-157 by caspase-8. Cellular cleavage of OPN is observed in apoptotic cells exposed to Hyp/RO among various apoptotic stimuli and its cleavage is blocked by zVAD or IETD caspase inhibitor. Further, over-expression of OPN, the form with secretion signal, inhibits Hyp/RO-induced cell death. Caspase cleavage-defective OPN mutant (OPN D135A/D157A) is more efficient to suppress Hyp/RO-induced cell death than wild-type OPN. OPN D135A/D157A sustains AKT activity to increase cell viability through inhibition of caspase-9 during Hyp/RO. In addition, OPN is highly induced in some tumor cells during Hyp/RO, such as HeLa and Huh-7 cells, which is associated with their resistance to Hyp/RO by sustaining AKT activity. Notably, OPN C-terminal cleavage fragment produced by caspase-8 is detected in the nucleus. Plasmid-encoded expression of OPN C-terminal cleavage fragment increases p53 protein level and induces apoptosis of wild-type mouse embryonic fibroblast cells, but not p53(-/-) mouse embryonic fibroblast cells. These observations suggest that the protective function of OPN during Hyp/RO is inactivated via the proteolytic cleavage by caspase-8 and its cleavage product subsequently induces cell death via p53, postulating caspase-8 as a negative regulator of tumorigenic activity of OPN.</P>

Recombinant agarase increases the production of reducing sugars from HCl-treated <i>Gracilaria verrucosa</i>, a red algae

Kim, Se Won,Kim, Yong-Woon,Hong, Chae-Hwan,Lyo, In-Woong,Lim, Ho-Dong,Kim, Geun-Joong,Shin, Hyun-Jae Elsevier 2018 Algal research Vol.31 No.-

<P><B>Abstract</B></P> <P>One of the most intensively studied species in red algae is <I>Gracilaria</I> spp., which is also a highly abundantly produced species annually in Asian countries including Korea and Japan. Much of the recent work on production of reducing sugars from <I>Gracilaria</I> spp. has concentrated on a combined process of physicochemical and biochemical treatments using acids and commercial enzymes. To the best of our knowledge, this study is first demonstration of combined acid and enzymatic hydrolysis using recombinant agarase to produce total reducing sugar (TRS) from <I>Gracilaria verrucosa</I>. By performing acid hydrolysis under the specified condition (2% biomass, 0.1N HCl, 121°C, 15min), a 34.9% TRS yield was obtained. Subsequent enzymatic hydrolysis using two recombinant agarases (aga 50D 0.5U/mg+NABH 0.5U/mg), resulted in a 47.4% TRS yield was achieved. The TRS yield obtained from acid and enzymatic hydrolysates was 5% higher than that of previous study with commercial enzyme. These results served to demonstrate that feasibility of enzymatic hydrolysis using the recombinant agarases to produce TRS from <I>G. verrucosa</I> for bio-refinery applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recombinant agarases were used to produce reducing sugar from <I>Gracilaria verrucosa</I>. </LI> <LI> Three agarases from <I>Saccharophagus degradans</I> were successfully expressed in <I>E. coli.</I> </LI> <LI> A higher yield (47.4%) of reducing sugar was obtained than with commercial enzyme. </LI> <LI> This study proposes combined hydrolysis using weak acids and recombinant enzymes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

What are ‘true normal’ liver stiffness values using FibroScan^®?: a prospective study in healthy living liver and kidney donors in South Korea

Kim, Seung Up,Choi, Gi Hong,Han, Woong Kyu,Kim, Beom Kyung,Park, Jun Yong,Kim, Do Young,Choi, Jin Sub,Yang, Seung Choul,Choi, Eun Hee,Ahn, Sang Hoon,Han, Kwang-Hyub,Chon, Chae Yoon Blackwell Publishing Ltd 2010 Liver International Vol.30 No.2

<P>Abstract</P><P>Aims</P><P>To identify the normal range of liver stiffness (LS) values by recruiting healthy living liver and kidney donors in South Korea.</P><P>Methods</P><P>Liver stiffness measurement (LSM) was performed in 69 healthy living liver and kidney donors who were admitted for transplantation. None of the subjects suffered from diabetes mellitus, hypertension, hepatitis B virus infection, hepatitis C virus infection, heart dysfunction, liver dysfunction or metabolic syndrome.</P><P>Results</P><P>LSM failure rate was 2.7%. Among 12 liver donors (17.4%) with available liver histology, eight showed normal liver histology and four showed liver steatosis of <5% of the hepatocytes. The mean age of our study population was 38.9 ± 11.9 years (35 men and 34 women), with a mean LS value of 4.6 ± 0.5 kPa (range 3.3–5.6 kPa). LS values were not significantly different between men (4.6 ± 0.6 kPa) and women (4.5 ± 0.5 kPa, <I>P</I>=0.636), nor were they significantly different according to age (<I>P</I>=0.851). Using the fifth and 95th percentiles, we determined the normal range of LS values to be 3.9–5.3 kPa.</P><P>Conclusions</P><P>We identified the normal range of LS values and found that LS values were not significantly influenced by age and gender.</P>

Influence of grain boundaries and voids on the saturated magnetization in Fe₃O₄ films at a low magnetic field

Kim, Ki Woong,Yoon, Kap Soo,Koo, Ja Hyun,Do, Young Ho,Kim, Chae Ok,Hong, Jin Pyo IEEE 2006 IEEE transactions on magnetics Vol.42 No.5

We report the magnetic behaviors of Fe<SUB>3</SUB>O<SUB>4</SUB> thin films grown by zero field growth (ZFG) and field growth (FG) techniques during the sputtering process. In FG conditions, an in situ 300 Oe field during growth is applied to a substrate, inducing an easy axis of magnetization. Structural observations obtained by high-resolution transmission electron microscopy measurements clearly depicted a significant reduction of the grain boundaries and voids in the Fe<SUB>3</SUB>O<SUB>4</SUB> films grown under FG conditions, thus explaining the saturated magnetization of the Fe<SUB>3</SUB>O<SUB>4</SUB> films at about 0.01 T. This behavior was expected due to a remarkable reduction of the antiferromagnetic exchange couplings between grains for FG conditions. In addition, the zero-field-cooled magnetization of the ZFG samples showed an abrupt change at about 285 K, confirming the existence of defects or other phases in the ZFG films.

Characterization of Flexible CIGS Thin Film Solar Cells on Stainless Steel with Intrinsic ZnO Diffusion Barriers

Kim, Chae-Woong,Kim, Hye Jin,Kim, Jin Hyeok,Jeong, Chaehwan American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.5

<P>ZnO diffusion barrier layer was deposited by RF magnetron sputtering by using the same method as intrinsic ZnO layer. The CIGS solar cells were fabricated on stainless steel substrate. The 50 similar to 200 nm thin ZnO diffusion barriers effectively reduced the diffusion of Fe and Cr, from stainless steel substrates into the CIGS absorbers. The CIGS solar cells with ZnO diffusion barriers increased the J(sc) and FF, which resulted in an increase of cell efficiency from 5.9% up to 9.06%.</P>

Predictors of poor clinical outcomes after successful chronic total occlusion intervention with drug-eluting stents

Kim, Gwang-Sil,Kim, Byeong-Keuk,Shin, Dong-Ho,Kim, Jung-Sun,Hong, Myeong-Ki,Gwon, Hyeon-Cheol,Kim, Hyo-Soo,Yu, Cheol Woong,Park, Hun Sik,Chae, In-Ho,Rha, Seung-Woon,Jang, Yangsoo Wolters Kluwer Health, Inc. All rights reserved. 2017 Coronary artery disease Vol.28 No.5

<P>Conclusion Clinical parameters such as age, diabetes, and heart failure were independent predictors of the composite of cardiac death, myocardial infarction, and stent thrombosis, whereas angiographic or procedural parameters such as lesion length and number of implanted stents were predictors of target-vessel revascularization. Clinical outcomes after CTO intervention were worse in patients with multiple risk factors. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.</P>