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The Membrane-Bound Form of IL-17A Promotes the Growth and Tumorigenicity of Colon Cancer Cells
Van Anh Do Thi,박상민,Hayyoung Lee,김영상 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.7
Interleukin-17A is a member of the IL-17 family, and is known as CTLA8 in the mouse. It is produced by T lym-phocytes and NK cells and has proinflammatory roles, inducing cytokine and chemokine production. However, its role in tumor biology remains controversial. We investigated the effects of locally produced IL-17A by transferring the gene encoding it into CT26 colon cancer cells, either in a secretory or a membrane-bound form. Expression of the membrane-bound form on CT26 cells dramatically enhanced their proliferation in vitro. The enhanced growth was shown to be due to an increased rate of cell cycle progression: after synchronizing cells by adding and withdrawing colcemid, the rate of cell cycle progression in the cells expressing the membrane-bound form of IL-17A was much faster than that of the control cells. Both secretory and membrane-bound IL-17A induced the expression of Sca-1 in the cancer cells. When tumor clones were grafted into syngeneic BALB/c mice, the tumor clones expressing the membrane-bound form IL-17A grew rapidly; those expressing the secretory form also grew faster than the wild type CT26 cells, but slower than the clones expressing the membrane-bound form. These results indicate that IL-17A promotes tumorigenicity by enhancing cell cycle progression. This finding should be considered in treating tumors and immune-related diseases.
The Membrane-Bound Form of IL-17A Promotes the Growth and Tumorigenicity of Colon Cancer Cells
Thi, Van Anh Do,Park, Sang Min,Lee, Hayyoung,Kim, Young Sang Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.7
Interleukin-17A is a member of the IL-17 family, and is known as CTLA8 in the mouse. It is produced by T lymphocytes and NK cells and has proinflammatory roles, inducing cytokine and chemokine production. However, its role in tumor biology remains controversial. We investigated the effects of locally produced IL-17A by transferring the gene encoding it into CT26 colon cancer cells, either in a secretory or a membrane-bound form. Expression of the membrane-bound form on CT26 cells dramatically enhanced their proliferation in vitro. The enhanced growth was shown to be due to an increased rate of cell cycle progression: after synchronizing cells by adding and withdrawing colcemid, the rate of cell cycle progression in the cells expressing the membrane-bound form of IL-17A was much faster than that of the control cells. Both secretory and membrane-bound IL-17A induced the expression of Sca-1 in the cancer cells. When tumor clones were grafted into syngeneic BALB/c mice, the tumor clones expressing the membrane-bound form IL-17A grew rapidly; those expressing the secretory form also grew faster than the wild type CT26 cells, but slower than the clones expressing the membrane-bound form. These results indicate that IL-17A promotes tumorigenicity by enhancing cell cycle progression. This finding should be considered in treating tumors and immune-related diseases.
Cell-Based IL-15:IL-15Rα Secreting Vaccine as an Effective Therapy for CT26 Colon Cancer in Mice
Thi, Van Anh Do,Jeon, Hyung Min,Park, Sang Min,Lee, Hayyoung,Kim, Young Sang Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.12
Interleukin (IL)-15 is an essential immune-modulator with high potential for use in cancer treatment. Natural IL-15 has a low biological potency because of its short half-life and difficulties in mass-production. IL-15Rα, a member of the IL-15 receptor complex, is famous for its high affinity to IL-15 and its ability to lengthen the half-life of IL-15. We have double-transfected IL-15 and its truncated receptor IL-15Rα into CT26 colon cancer cells to target them for intracellular assembly. The secreted IL-15:IL-15Rα complexes were confirmed in ELISA and Co-IP experiments. IL-15:IL-15Rα secreting clones showed a higher anti-tumor effect than IL-15 secreting clones. Furthermore, we also evaluated the vaccine and therapeutic efficacy of the whole cancer-cell vaccine using mitomycin C (MMC)-treated IL-15:IL-15Rα secreting CT26 clones. Three sets of experiments were evaluated; (1) therapeutics, (2) vaccination, and (3) long-term protection. Wild-type CT26-bearing mice treated with a single dose of MMC-inactivated secreted IL-15:IL-15Rα clones prolonged survival compared to the control group. Survival of MMC-inactivated IL-15:IL-15Rα clone-vaccinated mice (without any further adjuvant) exceeded up to 100%. This protection effect even lasted for at least three months after the immunization. Secreted IL-15:IL-15Rα clones challenging trigger anti-tumor response via CD4<SUP>+</SUP> T, CD8<SUP>+</SUP> T, and natural killer (NK) cell-dependent cytotoxicity. Our result suggested that cell-based vaccine secreting IL-15:IL-15Rα, may offer the new tools for immunotherapy to treat cancer.
Crosstalk between the Producers and Immune Targets of IL-9
Van Anh Do-Thi,Jie-Oh Lee,이혜영,김영상 대한면역학회 2020 Immune Network Vol.20 No.6
IL-9 has been reported to play dual roles in the pathogenesis of autoimmune disorders and cancers. The collaboration of IL-9 with microenvironmental factors including the broader cytokine milieu and other cellular components may provide important keys to explain its conflicting effects in chronic conditions. In this review, we summarize recent findings on the cellular sources of, and immunological responders to IL-9, in order to interpret the role of IL-9 in the regulation of immune responses. This knowledge will provide new perspectives to improve clinical benefits and limit adverse effects of IL-9 when treating pathologic conditions.
Do Thi, Van Anh,Park, Sang Min,Lee, Hayyoung,Kim, Young Sang 한국조명·전기설비학회 2018 한국조명·전기설비학회 학술대회논문집 Vol. No.
<P>IL-9 is a known T cell growth factor with pleiotropic immunological functions, especially in parasite infection and colitis. However, its role in tumor growth is controversial. In this study, we generated tumor clones expressing the membrane-bound form of IL-9 (MB-IL-9) and investigated their influences on immune system. MB-IL-9 tumor clones showed reduced tumorigenicity but shortened survival accompanied with severe body weight loss in mice. MB-IL-9 expression on tumor cells had no effect on cell proliferation or major histocompatibility complex class I expression <I>in vitro</I>. MB-IL-9 tumor clones were effective in amplifying CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cells and increasing cytotoxic activity against CT26 cells <I>in vivo</I>. We also observed a prominent reduction in body weights and survival period of mice injected intraperitoneally with MB-IL-9 clones compared with control groups. Ratios of IL-17 to interferon (IFN)-γ in serum level and tumor mass were higher in mice implanted with MB-IL-9 tumor clones than those observed in mice implanted with control cells. These results indicate that the ectopic expression of the MB-IL-9 on tumor cells exerts an immune-stimulatory effect with toxicity. To exploit its benefits as a tumor vaccine, a strategy to control the toxicity of MB-IL-9 tumor clones should be developed.</P>
Relaxor Behaviors in xBaTiO₃–(1-x)CoFe₂O₄ Materials
Cao Thi My Dung,Nhu Hoa Tran Thi,Kieu Hanh Thi Ta,Vinh Cao Tran,Bao Thu Le Nguyen,Van Hieu Le,Phuong Anh Do,Anh Tuan Dang,Heongkyu Ju,Bach Thang Phan 한국자기학회 2015 Journal of Magnetics Vol.20 No.4
Dielectric properties of xBaTiO₃–(1-x)CoFe₂O₄ composite materials have been investigated. Dielectric properties of BaTiO₃, CoFe₂O₄ and 0.5BaTiO₃–0.5CoFe₂O₄ samples show frequency dependence, which is classified as relaxor behavior with different relaxing degree. The relaxor behaviors were described using the modified Curier-Weiss and Vogel–Fulcher laws. Among three above samples, the BaTiO₃ sample has highest relaxing degree. Photoluminescence spectral indicated defects, which might in turn control relaxing degree.
The complete chloroplast genome of purple longan (Dimocarpus longan Lour., Sapindaceae)
Pham Anh Thi Nguyen,Tan Khang Do,Thi Pha Nguyen,Van Be Nam Tran,Hoang Dang Khoa Do 국립중앙과학관 2024 Journal of Asia-Pacific Biodiversity Vol.17 No.1
Purple longan (Dimocarpus longan Lour.) is a new longan cultivar that arose in Soc Trang province,Vietnam. Although the economic value of purple longan has been revealed, genomic data have not beenexplored. In this study, the complete chloroplast genome of purple longan was sequenced and charac terized, which was 160,763 bp in length. The genome had a quadripartite structure, including a largesingle copy (85,628 bp), a small single copy (18,247 bp), and two inverted repeat (28,444 bp) regions. This genome contained 79 protein-coding genes, 30 transfer RNAs, and four ribosomal RNAs, of which, 16genes were duplicated in inverted repeat regions. Comparative genomic analysis revealed 11 highlyvariable regions (including matK-trnK_UUU, psbK-trnG_UCC, atpH-atpI, psbZ-rps14, pafI-trnL_UAA, psbJ petG, clpP, rpl36-rpl14, rpl32-ccsA, rps15-ycf1, and ycf1). Additionally, 100 insertion and deletion sites wereidentified in the purple longan chloroplast genome. This study provided the first report on the completechloroplast genome of purple longan, which is useful for further studies on cultivar identification,germplasm conservation, and plant breeding in the Dimocarpus genus and Sapindaceae family.
New Alkaloids and Anti-inflammatory Constituents from the Leaves of Antidesma ghaesembilla
Van Kiem, Phan,Cuong, Le Canh Viet,Trang, Do Thi,Nhiem, Nguyen Xuan,Le Tuan Anh, Hoang,Tai, Bui Huu,Huong, Le Mai,Van Minh, Chau,Lee, Taek Hwan,Kim, Sun Yeou NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.1