Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

( Zhuang Ding ),( Tao Tao ),( Lili Wang ),( Yanna Zhao ),( Huiming Huang ),( Demeng Zhang ),( Min Liu ),( Zhengping Wang ),( Jun Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5

Endophytic fungi are an important component of plant microbiota, and have the excellent capacity for producing a broad variety of bioactive metabolites. These bioactive metabolites not only affect the survival of the host plant, but also provide valuable lead compounds for novel drug discovery. In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi was evaluated against five pathogenic microorganisms. Two strains, Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum), displayed broad-spectrum bioactivities. Our following study emphasizes the isolation, identification and bioactivity testing of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3-5) were isolated, and their structures were elucidated using NMR and MS. Compounds 1 and 2 exhibited inhibitory activity against Escherichia coli. Our findings reveal that endophytic fungi from the rubber tree F. elastica leaves exhibit unique characteristics and are potential producers of novel natural bioactive products.

Design of a Modular Green Closed Internal Cooling Turning Tool for Applications

Tao Wu,Tianjian Li,Xiaohong Ding,Hong Chen,Lei Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.5 No.2

Green machining has been aggressively pursued in the manufacturing industry in recent years. Dry or clean cutting without the hazards of cooling liquid, and their abilities to monitor thermal impact on turning tools in real time have been very appealed to the manufacturing industries. In this study, a modular green closed internal cooling turning tool is presented, in which coolant channel is designed only between an insert and an adaptor. The insert is grouped with the adaptor and designed as a Plug-and-Play component in cooling circuit. Thermal-fluid-structural coupling analysis is employed to evaluate the effects of the internal cooling system with machining process constraints. The numerical simulation results demonstrate that the newly designed internal cooing turning tool could effectively decrease the tool temperature. Preliminary experiments have further proved the effectiveness of the modular green closed internal cooling turning tool system. The study has potentially great significance in improving tooling performance, as well as achieving environmental friendly, economical and sustainable machining.

Behavior of polygonal concrete-filled steel tubular stub columns under axial loading

Tao Zhang,Fa-Xing Ding,Liping Wang,Xue-mei Liu,Guo-shuai Jiang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.5

The objective of this paper is to investigate the mechanical performances of polygonal concrete-filled circular steel tubular (CFT) stub columns under axial loading through combined experimental and numerical study. A total of 32 specimens were designed to investigate the effect of the concrete strength and steel ratio on the compressive behavior of polygonal CFT stub columns. The ultimate bearing capacity, ductility and confinement effect were analyzed based on the experimental results and the failure modes were discussed in detail. Besides, ABAQUS was adopted to establish the three dimensional FE model. The composite action between the core concrete and steel tube was further discussed and clarified. It was found that the behavior of CFT stub column changes with the change of the cross-section, and the change is continuous. Finally, based on both experimental and numerical results, a unified formula was developed to estimate the ultimate bearing capacity of polygonal CFT stub columns according to the superposition principle with rational simplification. The predicted results showed satisfactory agreement with both experimental and FE results.

Expression Characteristics of Proteins of the Insulin-like Growth Factor Axis in Non-small Cell Lung Cancer Patients with Preexisting Type 2 Diabetes Mellitus

Ding, Jing,Tang, Jie,Chen, Xin,Men, Hai-Tao,Luo, Wu-Xia,Du, Yang,Ge, Jun,Li, Cong,Chen, Ye,Cheng, Ke,Qiu, Meng,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Preexisting type 2 diabetes mellitus (T2DM) affects the prognosis and mortality of patients with some cancers. Insulin like growth factor (IGF) and insulin receptor (IR) signaling axes play important roles in both cancer and diabetes development. We aimed to explore the expression characteristics of proteins in IGF/IR axis in non-small cell lung cancer (NSCLC) cases with preexisting T2DM. Methods: Fifty-five NSCLC patients with preexisting T2DM were retrospectively included and matched by 55 NSCLC without diabetes at a 1:1 ratio. The expression of proteins in IGF/IR axis was detected by immunohistochemical staining. Clinicopathological data were collected to analyze their relationship with the protein expression. Results: Both IGF 1 receptor (IGF-1R) and insulin receptor substrate 2 (IRS-2) showed higher expression in the NSCLC with T2DM group, compared with those without T2DM. The high expression of IGF-1R and IRS-2 were found to be negatively associated with lymph node metastases and T staging in the T2DM group, respectively, and IRS-2 expression was also found more in the subgroup whose T2DM duration was more than 4 years. No difference was detected in the expression of IRS-1, IGF-1, IGF-2, IGFBP3, IR and mTOR between groups with or without T2DM. Conclusion: Our study found higher expression of IGF-1R and IRS-2 proteins in NSCLC patients with preexisting T2DM, and that there was an association with early stage NSCLC, which suggested that IGF signaling may play an important early event in development of NSCLC associated with diabetes.

Icariside II Promotes the Differentiation of Adipose Tissue-Derived Stem Cells to Schwann Cells to Preserve Erectile Function after Cavernous Nerve Injury

Tao Zheng,Tian-biao Zhang,Chao-liang Wang,Wei-xing Zhang,Dong-hui Jia,Fan Yang,Yang-yang Sun,Xiao-ju Ding,Rui Wang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.6

Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection. A Dual-Luciferase Reporter Assay was performed to determine the interaction between miR-34a and STAT3. Rat models with bilateral cavernous nerve injuries (BCNIs) were used to assess erectile function through the detection of mean arterial pressure (MAP) and intracavernosal pressure (ICP). ICA II promoted ADSCs’ proliferation and differentiation to Schwann cells (SCs) through the inhibition of miR-34a. Suppressed miR-34a promoted the differentiation of ADSCs to SCs by up-regulating STAT3. ICA II promoted the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway. The combination of ICA II and ADSCs preserved the erectile function of the BCNI model rats. ADSCs treated with ICA II markedly preserved the erectile function of the BCNI model rats, which was reversed through miR-34a overexpression. ICA II pro-motes the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway, contributing to erectile function preservation after the occurrence of a cavernous nerve injury.

Characteristic of SiC Slurry in Ultra Precision Lapping of Sapphire Substrates

Tao Yin,ZhiDa Wang,Toshiro Doi,Syuhei Kurokawa,Zhe Tan,XiaoKang Ding,Huan Lin 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.6

A method is proposed in this paper to prepare a SiC slurry with SiC particles selected by an ultrasonic-assisted elutriation method to reduce substrate surface damage caused by abrasive particles during lapping. Sapphire substrate lapping experiments were carried out using the prepared SiC slurry, and the lapping performance of the slurry was analyzed. The experimental results show that the SiC particle size is a factor that directly affects the material removal rate and surface roughness Ra, of sapphire substrates. When a SiC slurry with a particle size of 630 nm was used, the material removal rate was 508 nm/h, and the surface roughness Ra was 1.9 nm; increasing the slurry concentration and the platen rotating speed can improve the material removal rate. In addition, the agglomeration of SiC particles in the slurry depends on the pH of the slurry. Efficient precision lapping of sapphire substrates can be achieved by selecting appropriately sized SiC particles and by adjusting the slurry pH to control the agglomeration and dispersion of SiC particlesto further reduce the scratches on the substrate surface during the lapping process.

Construction and analysis of Sip1Aa insecticidal protein random recombination library

Ding Yue-ming,Wang Jing,Wang Lin,Gao Ji-Guo,Liu Rong-Mei,Li Hai-Tao 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2

The Sip1Aa protein from Bacillus thuringiensis is highly toxic to Colaphellus bowringi Baly. In order to obtain mutant proteins with higher insecticidal activity, a random recombinant library of Sip1Aa protein was con structed using error-prone PCR. A total number of 100 positive transformants were randomly selected for sequence determination, and 25 mutants (M1 to M25) were selected and expressed the respective Sip1Aa mu tants. These Sip1Aa variants had a total of 29 base mutations, with an average of 1.2 base mutations per mutant. Compared with that of the wild-type Sip1Aa protein, the insecticidal activity of the mutants M1 (A31G, Y118C, D227E), M5 (K168R) and M21 (I307T) was significantly decreased, with and LC 50 values 4 to 6 times higher than the Sip1Aa protein. The mutant M8 (R174S) showed increase in the insecticidal activity against the Colaphellus bowringi Baly was obtained, with an LC 50 value 4-fold less than the Sip1Aa protein. The results of this study provide reference for the molecular modification of Sip1Aa protein and the study of key sites of its insecticidal activity.

Study of the structural damage identification method based on multi-mode information fusion

Tao Liu,AiQun Li,YouLiang Ding,DaLiang Zhao 국제구조공학회 2009 Structural Engineering and Mechanics, An Int'l Jou Vol.31 No.3

Due to structural complicacy, structural health monitoring for civil engineering needs more accurate and effectual methods of damage identification. This study aims to import multi-source information fusion (MSIF) into structural damage diagnosis to improve the validity of damage detection. Firstly, the essential theory and applied mathematic methods of MSIF are introduced. And then, the structural damage identification method based on multi-mode information fusion is put forward. Later, on the basis of a numerical simulation of a concrete continuous box beam bridge, it is obviously indicated that the improved modal strain energy method based on multi-mode information fusion has nicer sensitivity to structural initial damage and favorable robusticity to noise. Compared with the classical modal strain energy method, this damage identification method needs much less modal information to detect structural initial damage. When the noise intensity is less than or equal to 10%, this method can identify structural initial damage well and truly. In a word, this structural damage identification method based on multi-mode information fusion has better effects of structural damage identification and good practicability to actual structures.

Variants on ESR1 and their Association with Prostate Cancer Risk: A Meta-analysis

Ding, Xiang,Cui, Feng-Mei,Xu, Song-Tao,Pu, Jin-Xian,Huang, Yu-Hua,Zhang, Jiang-Lei,Wei, Xue-Dong,Hou, Jian-Quan,Yan, Chun-Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. Methods: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. Results: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). Conclusion: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.

Nitrogen Fixation by Ru Single-Atom Electrocatalytic Reduction

Tao, Hengcong,Choi, Changhyeok,Ding, Liang-Xin,Jiang, Zheng,Han, Zishan,Jia, Mingwen,Fan, Qun,Gao, Yunnan,Wang, Haihui,Robertson, Alex W.,Hong, Song,Jung, Yousung,Liu, Shizhen,Sun, Zhenyu Elsevier 2019 Chem Vol.5 No.1