Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

Wang, Chao,Ma, Hong-Xi,Jin, Mei-Shan,Zou, Ya-Bin,Teng, Yong-Liang,Tian, Zhuang,Wang, Hai-Ying,Wang, Yin-Ping,Duan, Xiu-Mei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Matrix metalloproteinase (MMP)-2 and MMP-9 are important proteases involved in invasion and metastasis of various tumors. Extra-gastrointestinal stromal tumors (EGISTs) are rare neoplasms. This study was performed to assess MMP-2 and MMP-9 expression in EGIST tissue samples for association with clinicopathological data from the patients. Twenty-one surgical EGIST tissue specimens were collected for analysis of MMP-2 and MMP-9 expression using immunohistochemistry. MMP-2 and MMP-9 proteins were expressed in all of the epithelial cell types of EGISTs, whereas they were only expressed in 75% of the spindle cell type, although there was no statistically significant difference (p>0.05). Expression of MMP-2 and MMP-9 proteins was associated with tumor size, mitotic rate, tumor necrosis, and distant metastasis (p<0.05). MMP-2 expression was linked with MMP-9 levels (p<0.05). However, there was no correlation between MMP-9 expression and age, sex, primary site, or cell morphology in any of these 21 EGIST patients (p>0.05). Moreover, expression of MMP-2 and MMP-9 proteins increased with the degree of EGIST risk. This study provided evidence of an association of MMP-2 and MMP-9 expression with advanced EGIST behavior.

Is Surgical Resection Justified for Hepatocellular Carcinoma with Portal Vein Tumor Thrombus? (A Systematic Review and Meta-Analysis)

( Liang Lei ),( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Zhen-li Li ),( Jun Han ),( Han Zhang ),( Hao Xing ),( Han Wu ),( Ming-da Wang ),( Chao Li ),( Zheng Wang ),( Feng Shen ),( Meng-chao Wu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The prognosis of hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is very poor. According to the BCLC treatment recommendations, sorafenib or other palliative treatment (PT) is recommended as the first-line therapy when it happens. In real world, however, a significant number of selected patients with HCC and PVTT suffered from surgical resection (SR). Methods: PubMed, Embase, Medline and Cochrane library were searched for studies comparing SR with PT (including TACE, sorafenib, etc.) for HCC with PVTT, which were published before September 2017. Results: 4,810 patients from 7 studies were enrolled in this meta-analysis, which divided into the SR group (n = 2,344) and the PT group (n = 2476). When compared with the PT group, the pooled hazard ratio (HR) for the 1, 3 and 5-year OS rates of the SR group were 0.56 (95% CI 0.52-0.60, P=0.03), 0.56 (95% CI 0.53-0.59, P<0.001) and 0.55 (95% CI 0.54-0.57, P<0.001). For subgroup analysis, when compared with the mere TACE group, the pooled HR for the 1, 3 and 5-year OS rates of the SR group were 0.54 (95% CI 0.43-0.67, P=0.81), 0.75 (95% CI 0.65-0.87, P=0.25) and 0.76 (95% CI 0.67-0.88, P=0.25). Conclusions: This meta-analysis demonstrated SR had better OS than TACE or other palliative therapy for HCC with PVTT. SR may be suitable as the first-line treatment for selected patients with resectable HCC and removable PVTT.

Mechanical Stretch Promotes Invasion of Lung Cancer Cells via Activation of Tumor Necrosis Factor-alpha

Liang-Kun Chen,Ching-Chi Hsieh,Yi-Chao Huang,Yuan-Jung Huang,Chun-Fan Lung,Wei-En Hsu,Chao-Ling Yao,Tsung-Yu Tseng,Chi-Chung Wang,Yi-Chiung Hsu 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3

Most of the gas exchange in the human body is carried out by the lungs, and the physiological activities of the lungs are uninterrupted. Due to the deterioration of the external environment, pulmonary cell lesions are common clinical lung diseases. Mechanical cyclic stretching is one kind of bionic technology to observe lung cancer cells. The A549 cell line is the human lung adenocarcinoma cell line derived from a primary lung tumor. This study investigated the effects of mechanical cyclic stretching on A549 cell activity and gene expression profile. Whereas mechanical cyclic stretching had no significant difference in colony formation and cell migration of A549 cells, the cell invasion increased significantly in A549 cells after stretching. In addition, the microarray data showed that mechanical cyclic stretching altered gene expression, induced inflammation of cells, and activation of Wnt/β- catenin and tumor necrosis factor pathways. More importantly, mechanical cyclic stretching activated the expression of tumor necrosis factor-alpha (TNF-α) protein. Therefore, the increase of cell invasion induced by mechanical cyclic stretching might be associated with the activation of TNF-α in human lung adenocarcinoma cells.

Multi-institutional development and validation of modified albumin-bilirubin grade for predicting prognosis of hepatocellular carcinoma patients after hepatectomy

Chao LI,Ming-Da WANG,Lei LIANG,Hao XING,Lan-Qing YAO,Zi-Xiang CHEN,Feng SHEN,Dong-Sheng HUANG,Tian YANG 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.-

Numerical prediction analysis of propeller bearing force for full-scale hull-propeller-rudder system

Wang, Chao,Sun, Shuai,Li, Liang,Ye, Liyu The Society of Naval Architects of Korea 2016 International Journal of Naval Architecture and Oc Vol.8 No.6

The hybrid grid was adopted and numerical prediction analysis of propeller unsteady bearing force considering free surface was performed for mode and full-scale KCS hull-propeller-rudder system by employing RANS method and VOF model. In order to obtain the propeller velocity under self-propulsion point, firstly, the numerical simulation for self-propulsion test of full-scale ship is carried out. The results show that the scale effect of velocity at self-propulsion point and wake fraction is obvious. Then, the transient two-phase flow calculations are performed for model and full-scale KCS hull-propeller-rudder systems. According to the monitoring data, it is found that the propeller unsteady bearing force is fluctuating periodically over time and full-scale propeller's time-average value is smaller than model-scale's. The frequency spectrum curves are also provided after fast Fourier transform. By analyzing the frequency spectrum data, it is easy to summarize that each component of the propeller bearing force have the same fluctuation frequency and the peak in BFP is maximum. What's more, each component of full-scale bearing force's fluctuation value is bigger than model-scale's except the bending moment coefficient about the Y-axis.

Gender Differences Associated with Pain Characteristics and Treatment in Taiwanese Oncology Outpatients

Liang, Shu-Yuan,Wang, Tsae-Jyy,Wu, Shu-Fang,Chao, Ta-Chung,Chuang, Yeu-Hui,Tsay, Shiow-Luan,Tung, Heng-Hsin,Lee, Ming-Der Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

The purpose of this descriptive and comparative study was to examine gender differences relevant to pain intensity, opioid prescription patterns and opioid consumption in Taiwanese oncology outpatients. The 92 participants had been prescribed opioid analgesics for cancer-related pain at least once in the past week and were asked to complete the Brief Pain Inventory - Chinese questionnaire and to recall the dosage of each opioid analgesic that they had ingested within the previous 24 hours. For opioid prescriptions and consumption, all analgesics were converted to morphine equivalents. The results revealed a significant difference between males and female minimum pain thresholds (t = 2.38, p = 0.02) and current pain thresholds (t = 2.12, p = 0.04), with males reporting a higher intensity of pain than females. In addition, this study found that males tended to use prescribed opioid analgesics more frequently than females on the bases of both around the clock (ATC) (t = 1.90, p = 0.06) and ATC plus as needed (ATC + PRN) (t = 2.33, p = 0.02). However, there was no difference between males and females in opioid prescriptions on an ATC basis (t = 0.52, p = 0.60) or at an ATC + PRN basis (t = 0.40, p = 0.69). The results suggest that there may be a gender bias in the treatment of cancer pain, supporting the proposal of routine examination of the effect of gender on cancer pain management. These findings suggest that clinicians should be particularly aware of potential gender differences during pain monitoring and the consumption of prescribed opioid analgesics.

Numerical prediction analysis of propeller bearing force for full-scale hullepropellererudder system

Chao Wang,Shuai Sun,Liang Li,Liyu Ye 대한조선학회 2016 International Journal of Naval Architecture and Oc Vol.8 No.6

The hybrid grid was adopted and numerical prediction analysis of propeller unsteady bearing force considering free surface was performed for mode and full-scale KCS hullepropellererudder system by employing RANS method and VOF model. In order to obtain the propeller velocity under self-propulsion point, firstly, the numerical simulation for self-propulsion test of full-scale ship is carried out. The results show that the scale effect of velocity at self-propulsion point and wake fraction is obvious. Then, the transient two-phase flow calculations are performed for model and full-scale KCS hullepropellererudder systems. According to the monitoring data, it is found that the propeller unsteady bearing force is fluctuating periodically over time and full-scale propeller's time-average value is smaller than model-scale's. The frequency spectrum curves are also provided after fast Fourier transform. By analyzing the frequency spectrum data, it is easy to summarize that each component of the propeller bearing force have the same fluctuation frequency and the peak in BFP is maximum. What's more, each component of full-scale bearing force's fluctuation value is bigger than model-scale's except the bending moment coefficient about the Y-axis.

Experimental Investigation on the Bond Behavior of Deformed Bars in a Class of Structural Expanded Polystyrene (EPS) Concrete

Chao Li,Linchang Miao,Quan You,Lijian Lei,Xiaodong Liang,Junjie Wang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.12

The structural expanded polystyrene (EPS) concrete (above 35 MPa) has the potential to be used as metro track bed to reduce and isolate vibration, but its bond behavior is still unclear. This inhibits its application because it is often used in combination with reinforcing bar. In this paper, a class of structural EPS concrete (above 35 MPa) was developed. Based on this, a series of pull-out tests were conducted and the bond behavior of deformed bars in these structural EPS concretes was investigated. In addition, effects of rebar bond length and rebar diameter were analyzed in detail. Experimental results indicate that the structural EPS concrete suffers splitting failure more easily than the conventional concrete (CC). Increase of EPS replacement, rebar bond length or rebar diameter tends to cause the splitting failure of structural EPS concrete. Moreover, with increase of EPS replacement, the failure process is less explosive and more gradual. The stress-slip relationships of the most specimens show three stages, namely linear ascending, non-linear progression, and descending stages. The bond strength of structural EPS concrete is found depended linearly on the power function of its compressive strength, relative cover thickness (c/d) and relative rebar bond length (L/d), respectively. Accordingly, a bond strength prediction model was formulated, and it can precisely predict the bond strength of deformed bars in structural EPS concrete, specimens failing in splitting or splitting-pull out modes, and no fiber added. The results can provide reference for the design of structural EPS concrete.

Toll-Like Receptor 4 Mutation Protects Obese Mice Against Endothelial Dysfunction by Decreasing NADPH Oxidase Isoforms 1 and 4

Liang, Chao-Fan,Liu, Jacky TC,Wang, Yu,Xu, Aimin,Vanhoutte, Paul M. American Heart Association, Inc. 2013 Arteriosclerosis, thrombosis, and vascular biology Vol.33 No.4

<P><B>Objective—</B></P><P>To analyze the role of toll-like receptor 4 in modulating metabolism and endothelial function.</P><P><B>Approach and Results—</B></P><P>Type 2 diabetic mice with mutated toll-like receptor 4 (DWM) were protected from hyperglycemia and hypertension, despite an increased body weight. Isometric tension was measured in arterial rings with endothelium. Relaxations to acetylcholine were blunted in aortae and mesenteric arteries of Lepr<SUP>db/db</SUP> mice, but not in DWM mice; the endothelial NO synthase dimer/monomer ratio and endothelial NO synthase phosphorylation levels were higher in DWM preparations. These differences were abolished by apocynin. Contractions to acetylcholine (in the presence of L-NAME) were larger in carotid arteries from Lepr<SUP>db/db</SUP> mice than from DWM mice and were inhibited by indomethacin and SC560, demonstrating involvement of cyclooxygenase-1. The release of 6-ketoprostaglandin F<SUB>1α</SUB> was lower in DWM mice arteries, implying lower cyclooxygenase-1 activity. Apocynin, manganese(III) tetrakis(1-methyl-4-pyridyl) porphyrin, catalase, and diethyldithiocarbamate inhibited endothelium-dependent contractions. The mRNA and protein levels of NADPH oxidase isoforms NOX1 and NOX4 were downregulated in DWM mice arteries. The in vivo and in vitro administration of lipopolysaccharide caused endothelial dysfunction in the arteries of wild-type, but not toll-like receptor 4–mutated mice.</P><P><B>Conclusions—</B></P><P>Toll-like receptor 4 plays a key role in obesity and diabetes-associated endothelial dysfunction by increasing oxidative stress.</P>

Construction of a Protein-Protein Interaction Network for Chronic Myelocytic Leukemia and Pathway Prediction of Molecular Complexes

Zhou, Chao,Teng, Wen-Jing,Yang, Jing,Hu, Zhen-Bo,Wang, Cong-Cong,Qin, Bao-Ning,Lv, Qing-Liang,Liu, Ze-Wang,Sun, Chang-Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: Chronic myelocytic leukemia is a disease that threatens both adults and children. Great progress has been achieved in treatment but protein-protein interaction networks underlining chronic myelocytic leukemia are less known. Objective: To develop a protein-protein interaction network for chronic myelocytic leukemia based on gene expression and to predict biological pathways underlying molecular complexes in the network. Materials and Methods: Genes involved in chronic myelocytic leukemia were selected from OMIM database. Literature mining was performed by Agilent Literature Search plugin and a protein-protein interaction network of chronic myelocytic leukemia was established by Cytoscape. The molecular complexes in the network were detected by Clusterviz plugin and pathway enrichment of molecular complexes were performed by DAVID online. Results and Discussion: There are seventy-nine chronic myelocytic leukemia genes in the Mendelian Inheritance In Man Database. The protein-protein interaction network of chronic myelocytic leukemia contained 638 nodes, 1830 edges and perhaps 5 molecular complexes. Among them, complex 1 is involved in pathways that are related to cytokine secretion, cytokine-receptor binding, cytokine receptor signaling, while complex 3 is related to biological behavior of tumors which can provide the bioinformatic foundation for further understanding the mechanisms of chronic myelocytic leukemia.