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Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats
Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*
부산·경남지역 사찰에서 수집된 차나무(Camellia sinensis L.)잎 형태별 분류
제순자(Je, Soon-Ja)・,이용호(Lee, Yong-Ho)・,오주성(Oh, Ju-Sung3)・,정영수(Chung, Yong-Soo)・,정대수(Chung, Dae-Soo) 한국차학회 2007 한국차학회지 Vol.13 No.1
본 시험은 부산·경남지역 사찰 주변에 자생하고 있는(또는 30년 이상 재배되어 온) 차나무를 중심으로 14개 지역을 선정하여 산지별로 차나무 잎을 채취, 수집하여 잎 형태별 특성을 조사하였다. 차나무 잎의 형태적 특성 중 엽장(葉長) (12.04㎝), 엽폭(葉幅)(4.90㎝), 엽면적(葉面積)(41.33㎝)은 사천 배방사지 차나무 잎이 가장 크게 나타났으며, 가장 작게 나타난 곳은 부산 전등사 차나무가 엽장(6.30㎝)과 엽폭(2.48㎝)이 작으며, 엽면적은 거제 봉곡사(14.80㎝) 차나무가 가장 작게 나타났다. 엽병장(葉柄長)은 밀양의 엄광사지(0.57㎝)와 사천 배방사지(0.57㎝) 차나무 잎이 가장 길게 나타났으며, 거치수(鋸齒數)는 사천 배방사지(30.13개) 차나무였으며, 엽맥수(葉脈數)는 하동 쌍계사(11.27개) 차나무로 가장 많게 나타났다. 생엽중(生葉重)과 건엽중(乾葉重)은 사천 배방사지 차나무가 생엽중(0.81 g), 건엽중(0.34 g)으로 가장 무거웠으며, 가장 작은 지역은 부산 전등사가 생엽중(0.25 g)이며, 건엽중은 전등사와 범어사(0.10 g)가 가장 가볍게 나타났다. 차나무 잎의 형질간 상관관계는 수량성을 나타내는 건엽중은 엽장, 엽폭, 엽면적, 생엽중과 고도의 유의성을 보였으며 0.8이상의 매우 높은 유의차를 보였다. 본 연구 결과 사천 배방사지 차나무가 엽장(葉長), 엽폭(葉幅), 엽면적(葉面積), 엽병장(葉柄長), 거치수(鋸齒數), 생엽무게, 건조무게 등은 전체적으로 가장 크고 무거웠으며, 엽맥수(葉脈數)는 하동 쌍계사 주변의 차나무가 가장 많았다. 자생하는 차나무의 다양한 유전적 변이를 이용하여 유용한 유전자원으로 이용한다면 우량 다수성 계통의 신품종 개발 가능성이 높을 것으로 판단되며, 금후 자생지역별로 생육환경이나 지리적 특성도 검토 분석 한다면 기능성 신품종 개발 보급에 기초 자료로서 활용성이 높을 것으로 판단된다. We collected the tea leaves from the vicinity of 14 Buddhist temple located in Busan and Gyeongnam area where wild tea trees and more than 30 year old tee trees were commonly found. The characteristics of the tea leaves were surveyed. Among those tee leaves from 14 areas, Baebangsaji (Goseong) tea leaves had the highest value of leaf length (12.04 ㎝), leaf width(4.90 cm), and leaf area (41.33 ㎝). The tea leaves of Jeondeungsa (Busan) had the lowest value of leaf length (6.30 ㎝) and leaf width (2.48 ㎝). The tea leaves of Bonggoksa (Geoje) had the lowest value of leaf area (14.80 ㎝). The longest leaf stalk length was Umgwangsaji (Miryang) tea leaves (0.57 ㎝) and Baedangsaji (Goseong) tea leaf (0.57 ㎝). The largest No. of serrate was Baedangsaji (Goseong) tea leaves (30.13) and for leaf vein was Ssanggyesa (Hadong) as a number of 11.27. The heaviest weight of fresh leaf was Baedangsaji (Goseong) tea leaf (0.81 g) and, also, the dry tea leaf of Baedangsaji (Goseong) was the heaviest (0.34 g). The lightest weight of fresh leaf was Jeondeungsa (Busan) tea leaves (0.25 g) and the lightest weight of dry leaf was Jeondeungsa. In this study, leaf length, leaf width, leaf area, leaf stalk length, No. of serrate, weight of fresh leaf and weight of dry leaf value of the leaves collected from Baedangsaji (Goseong) was generally high and The highest value of No. of leaf vein was from Ssanggyesa (Hadong).
Lee, Sang-Yeop,Oh, Man Hwan,Yun, Sung Ho,Choi, Chi-Won,Park, Edmond Changkyun,Song, Hyun Seok,Lee, Hayoung,Yi, Yoon-Sun,Shin, Juhyun,Chung, Chaeuk,Moon, Jae Young,Lee, Je Chul,Kim, Gun-Hwa,Kim, Seung Elsevier 2018 INFECTION GENETICS AND EVOLUTION Vol.65 No.-
<P><B>Abstract</B></P> <P>Extensively drug-resistant (XDR) <I>Acinetobacter baumannii</I> strains have emerged rapidly worldwide. The antibiotic resistance characteristics of XDR <I>A. baumannii</I> strains show regional differences; therefore, it is necessary to analyze both genomic and proteomic characteristics of emerging XDR <I>A. baumannii</I> clinical strains isolated in Korea to elucidate their multidrug resistance. Here, we isolated new sequence type of XDR <I>A. baumannii</I> clinical strain (KAB03) from Korean hospitals and performed comprehensive genome analyses. The strain belongs to new sequence type, ST451. Single nucleotide polymorphism (SNP) analysis with other types of <I>A. baumannii</I> strains revealed that KAB03 has unique SNP pattern in the regions of <I>gyrB</I> and <I>gpi</I> of MLST profiles. <I>A. baumannii</I> KAB03 harbours three antibiotic resistance islands (AbGRI1, 2, and 3). AbGRI1 harbours two copies of Tn<I>2006</I> containing <I>bla</I> <SUB>OXA-23</SUB>, which play an important role in antibiotic resistance. AbGRI2 possesses aminoglycoside resistant gene <I>aph(3′)-Ic</I> and class A β-lactamase <I>bla</I> <SUB>TEM</SUB>. AbGIR3 has macrolide resistant genes and aminoglycoside resistant gene <I>armA</I>. <I>A. baumannii</I> KAB03 harbours mutations in <I>pmrB</I> and <I>pmrC</I>, which are believed to confer colistin resistance. In addition, proteomic and transcriptional analysis of KAB03 confirmed that β-lactamases (ADC-73 and OXA-23), Ade efflux pumps (AdeIJK), outer membrane proteins (OmpA and OmpW), and colistin resistance genes (PmrCAB) were major proteins responsible for antibiotic resistance. Our proteogenomic results provide valuable information for multi-drug resistance in emerging XDR <I>A. baumannii</I> strains belonging to ST451.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Whole genome of an XDR <I>A. baumannii</I> KAB03 belonging to ST451, isolated in South Korea, was analyzed </LI> <LI> <I>A.s baumannii</I> strains belonging to ST451 have unique SNP pattern in the regions of <I>gyrB</I> and <I>gpi</I> of MLST profiles </LI> <LI> Antibiotic resistance proteins of <I>A. baumannii</I> KAB03 were suggested by proteomic and transcriptomic analysis </LI> </UL> </P>
Lee, Chu,Chun, Wonjoo,Zhao, Rongjie,Kim, Young Dae,Nam, Myung Mo,Jung, Dae Hwa,Cho, Il Je,Jegal, Kyung Hwan,Lee, Tae Hoon,Kim, Young Woo,Park, Sang Mi,Ju, Seong A.,Lee, Chul Won,Kim, Sang Chan,An, Won D.A. Spandidos 2017 Oncology letters Vol.14 No.2
<P><I>Patinopecten yessoensis</I>, is a species of scallop and a marine bivalve mollusk. In traditional East Asian medicine, scallop meat is used as a drug for the treatment of diabetes, pollakisuria, and indigestion. The present study was conducted in order to examine the potential anticancer effects of scallop flesh extract (SE) on MCF-7 human breast cancer cells. An MTT assay was used to evaluate cell viability and flow cytometry was used for the assessment of cell cycle distribution and apoptosis. The alteration in protein expression level was determined by western blot analysis, and the amounts of docosahexaenoic acid and eicosapentaenoic acid in the SE were measured by gas chromatography. SE inhibited the growth of MCF-7 human breast cancer cells in a dose-dependent manner by inducing G0/G1 phase arrest. The cell cycle arrest was associated with the upregulation of p53 and p21, and downregulation of G1 phase-associated cyclin D1/cyclin-dependent kinase (Cdk) 4 and cyclin E1/Cdk 2. In addition, SE-mediated cell cycle arrest was associated with the promotion of apoptosis, as indicated by the expression of apoptosis-associated proteins and changes in nuclear morphology. SE appeared to induce the mitochondrial apoptotic cascade, as indicated by a decreased expression of Bcl-2, activation of Bcl-2 associated X protein, release of cytochrome c, decrease in procaspase-3, and an increase in cleaved-poly (ADP-ribose) polymerase (PARP). Furthermore, the expression levels of Fas-associated via death domain and cleaved caspase-8 were increased in a SE dose-dependent manner. Taken together, these results suggest that the intrinsic and extrinsic pathways of apoptosis are associated with the anticancer effects of SE on MCF-7 cells. Thus, SE may be a suitable candidate for the treatment and prevention of human breast cancer.</P>
Lee, Jinwoo,Lee, Dohoon,Oh, Eunkeu,Kim, Jaeyun,Kim, Young-Pil,Jin, Sunmi,Kim, Hak-Sung,Hwang, Yosun,Kwak, Ja Hun,Park, Je-Geun,Shin, Chae-Ho,Kim, Jungbae,Hyeon, Taeghwan WILEY-VCH Verlag 2005 Angewandte Chemie Vol.44 No.45
<B>Graphic Abstract</B> <P>Glucose oxidase (GOx) was immobilized in magnetic mesocellular carbon foam by cross-linking with glutaraldehyde. This material has been utilized to construct a magnetically switchable bio-electrocatalytic system in which the catalytic oxidation of glucose could be switched on and off by the positioning of an external magnet near and away from the electrode (see scheme). <img src='wiley_img/14337851-2005-44-45-ANIE200502995-content.gif' alt='wiley_img/14337851-2005-44-45-ANIE200502995-content'> </P>
Combination of honokiol and magnolol inhibits hepatic steatosis through AMPK-SREBP-1 c pathway
Lee, Ju-Hee,Jung, Ji Yun,Jang, Eun Jeong,Jegal, Kyung Hwan,Moon, Soo Young,Ku, Sae Kwang,Kang, Seung Ho,Cho, Il Je,Park, Sook Jahr,Lee, Jong Rok,Zhao, Rong Jie,Kim, Sang Chan,Kim, Young Woo SAGE Publications 2015 Experimental biology and medicine Vol.240 No.4
Lee, Ki-Sung,Kim, Jun-Seob,Heo, Paul,Yang, Tae-Jun,Sung, Young-Je,Cheon, Yuna,Koo, Hyun Min,Yu, Byung Jo,Seo, Jin-Ho,Jin, Yong-Su,Park, Jae Chan,Kweon, Dae-Hyuk Springer International 2013 Applied microbiology and biotechnology Vol.97 No.5
<P>Kluyveromyces marxianus is now considered one of the best choices of option for industrial applications of yeast because the strain is able to grow at high temperature, utilizes various carbon sources, and grows fast. However, the use of K. marxianus as a host for industrial applications is still limited. This limitation is largely due to a lack of knowledge on the characteristics of the promoters since the time and amount of protein expression is strongly dependent on the promoter employed. In this study, four well-known constitutive promoters (P(CYC), P(TEF), P(GPD), and P(ADH)) of Saccharomyces cerevisiae were characterized in K. marxianus in terms of protein expression level and their stochastic behavior. After constructing five URA3-auxotrophic K. marxianus strains and a plasmid vector, four cassettes each comprising one of the promoters--the gene for the green fluorescence protein (GFP)--CYC1 terminator (T(CYC)) were inserted into the vector. GFP expression under the control of each one of the promoters was analyzed by reverse transcription PCR, fluorescence microscopy, and flow cytometer. Using these combined methods, the promoter strength was determined to be in the order of P(GPD) > P(ADH) P(TEF) > P(CYC). All promoters except for the P(CYC) exhibited three distinctive populations, including non-expressing cells, weakly expressing cells, and strongly expressing cells. The relative ratios between populations were strongly dependent on the promoter and culture time. Forward scattering was independent of GFP fluorescence intensity, indicating that the different fluorescence intensities were not just due to different cell sizes derived from budding. It also excluded the possibility that the non-expressing cells resulted from plasmid loss because plasmid stability was maintained at almost 100 % over the culture time. The same cassettes, cloned into a single copy plasmid pRS416 and transformed into S. cerevisiae, showed only one population. When the cassettes were integrated into the chromosome, the stochastic behavior was markedly reduced. These combined results imply that the gene expression stochasticity should be overcome in order to use this strain for delicate metabolic engineering, which would require the co-expression of several genes.</P>