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Uterine metastatic rhabdomyosarcoma in a scimitar-horned oryx ( <i>Oryx dammah</i> )
GO, Du-Min,WOO, Sang-Ho,LEE, Su-Hyung,KWON, Soo-Whan,CHUNG, Dong-Hee,YOUN, Soong-Hee,JUNG, Eun,KIM, Dae-Yong The Japanese Society of Veterinary Science 2018 The Journal of veterinary medical science Vol.80 No.10
<P>A 13-year-old female scimitar-horned oryx (<I>Oryx dammah</I>) died after progressive anorexia, weight loss, and depression. The necropsy showed that the retroperitoneum was compressed by a large white-to-tan uterine mass and on several sections of the mass, the uterine wall was markedly thickened because of ill-defined transmural tumor tissue. Metastatic nodules were detected in the omentum, mesentery, diaphragm, and lung. The genital tract and pulmonary and abdominal nodules exhibited highly pleomorphic sarcoma. The primary and metastatic neoplastic cells showed positive results for vimentin, desmin, and sarcomeric actin, and negative results for smooth muscle actin. Uterine metastatic rhabdomyosarcoma was diagnosed on the basis of the gross, histopathology and immunohistochemistry results.</P>
Hee Sang Hwang,박윤용,Su-Jin Shin,Heounjeong Go,Ja-Min Park,Sun Young Yoon,Jae Lyun Lee,Yong Mee Cho 대한의학회 2020 Journal of Korean medical science Vol.35 No.5
Background: Mechanism and predictive biomarkers for tyrosine kinase inhibitor (TKI) resistance of advanced clear cell renal cell carcinoma (ccRCC) have not been fully evaluated. Methods: We performed gene expression profiling on samples from an acquired TKI resistance cohort that consisted of 10 cases of TKI-treated ccRCC patients with matched tumor tissues harvested at pre-treatment and TKI-resistant post-treatment periods. In addition, a public microarray dataset from patient-derived xenograft model for TKI-treated ccRCC (GSE76068) was retrieved. Commonly altered pathways between the datasets were investigated by Ingenuity Pathway Analysis using commonly regulated differently expressed genes (DEGs). The significance of candidate DEG on intrinsic TKI resistance was assessed through immunohistochemistry in a separate cohort of 101 TKI-treated ccRCC cases. Results: TNFRSF1A gene expression and tumor necrosis factor (TNF)-α pathway were upregulated in ccRCCs with acquired TKI resistance in both microarray datasets. Also, high expression (> 10% of labeled tumor cells) of TNF receptor 1 (TNFR1), the protein product of TNFRSF1A gene, was correlated with sarcomatoid dedifferentiation and was an independent predictive factor of clinically unfavorable response and shorter survivals in separated TKI- treated ccRCC cohort. Conclusion: TNF-α signaling may play a role in TKI resistance, and TNFR1 expression may serve as a predictive biomarker for clinically unfavorable TKI responses in ccRCC.
Analysis of Diapause-Associated Changes in Silkworm Egg Proteins
Go, Hyun-Jeong,Hwang, Jae-Sam,Kim, Young-Tae,Kim, Hyun-Su,Kang, Seok-Woo,Chang, Jong-Su,Lee, Sang-Mong,Lee, Bong-Hee,Seong, Su-Il Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.1
The patterns of diapause-associated proteins of silkworm eggs were analyzed by two-dimensional (2-D) gel electrophoresis. Among the hundreds of spots on the 2-D gels, at least two proteins were considered to be associated with diapause. A protein, spot 4, with an approximate molecular weight of 38 kDa and pI 6.1 was observed in the HCI-treated, cold-treated, and diapause eggs, respectively. Spot 4 was undetectable in unfertilized eggs and non-diapause eggs at two days after oviposition, suggesting that this protein may be associated with the entrance to diapause. A protein, spot 11, with an approximate molecular weight of 21 kDa and pI of 61 was detected in the unfertilized, HCl-treated, and cold-treated eggs, respectively, after oviposition by normal moths. In diapausing eggs, a protein corresponding to spot 11 was observed in 3-, 5-, and 30-day-old eggs, while the protein was not detected one-day-old eggs. The protein corresponding to spot 11 was not detected in unfertilized and non-diapause eggs obtained from subesophabeal ganglion (SG)-extirpated moths either. Spot 11 was also considered to be a diapause specific protein, which occurred at only early embryonic stage under the control of diapause-downregulated gene.
Resistive Switching Properties of Pt/TiO2/n+-Si ReRAM for Nonvolatile Memory Application
( Sang Hee Won ),( Seung Hee Go ),( Kwan Woo Lee ),( Jae Gab Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2008 ELECTRONIC MATERIALS LETTERS Vol.4 No.1
We fabricated sputter-Pt/atomic layer deposition (ALD) TiO2/n+-Si structures for resistive random access memory (ReRAM). The use of n+-Si bottom electrode provides a simple way of fabricating ReRAMs, and offers compatibility with the conventional CMOS process. After annealing Pt (100 nm)/ ALD TiO2(38 nm)/ n+-Si structures in an O2 ambient at a temperature range of 100 to 500°C, we examined their effects on the switching properties. The as-deposited ALD TiO2 thin films showed no switching behavior. However, annealing at 100°C enabled the thin films to switch between a low- resistance state and a high-resistance state, thereby revealing the resistive switching behavior. In addition, electric pulse-induced resistance switching was repeated (about twenty times) in the 100°C-annealed TiO2 films. When the annealing temperature was increased to 300°C, the switching properties of the TiO2 thin films were significantly improved in terms of the resistive switching cycle and the dispersion of the set/reset voltages, probably due to the improved crystallization of the TiO2 films and the enhanced anode interface properties. As a result, the simple structure of Pt/ALD TiO2/n+-Si can be applied to nonvolatile memory devices.
Sang-Yeon Lee,Hyun Been Choi,Mina Park,Il Soon Choi,Jieun An,Ami Kim,Eunku Kim,Nahyun Kim,Jin Hee Han,Min young Kim,Seung min Lee,Doo-Yi Oh,Bong Jik Kim,Nayoung Yi,Nayoung, K. D. Kim,Chung Lee,Woong-Y 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Loss-of-function variant in the gene encoding the KCNQ4 potassium channel causes autosomal dominant nonsyndromic hearing loss (DFNA2), and no effective pharmacotherapeutics have been developed to reverse channel activity impairment. Phosphatidylinositol 4,5-bisphosphate (PIP2), an obligatory phospholipid for maintaining KCNQ channel activity, confers differentialpharmacological sensitivity of channels to KCNQ openers. Through whole-exome sequencing of DFNA2 families, we identified three novel KCNQ4 variants related to diverse auditory phenotypes in the proximal C-terminus (p.Arg331Gln), the C-terminus of the S6 segment (p.Gly319Asp), and the pore region (p.Ala271_Asp272del). Potassium currents in HEK293T cells expressing each KCNQ4 variant were recorded by patch-clamp, and functional recovery by PIP2 expression or KCNQ openers was examined. In the homomeric expression setting, the three novel KCNQ4 mutant proteins lost conductance and were unresponsive to KCNQ openers or PIP2 expression. Loss of p.Arg331Gln conductance was slightly restored by a tandem concatemer channel (WT-p.R331Q), and increased PIP2 expression further increased the concatemer current to the level of the WT channel. Strikingly, an impaired homomeric p.Gly319Asp channel exhibited hyperactivity when a concatemer (WT-p.G319D), with a negative shift in the voltage dependence of activation. Correspondingly, a KCNQ inhibitor and chelation of PIP2 effectively downregulated the hyperactive WTp. G319D concatemer channel. Conversely, the pore-region variant (p.Ala271_Asp272del) was nonrescuable under any condition. Collectively, these novel KCNQ4 variants may constitute therapeutic targets that can be manipulated by the PIP2 level and KCNQregulating drugs under the physiological context of heterozygous expression. Our research contributes to the establishment of a genotype/mechanism-based therapeutic portfolio for DFNA2.
( Go Eun Yang ),( Dong Sun Park ),( Sun Hee Lee ),( Dae Kwon Bae ),( Yun Hui Yang ),( Jang Been Kyung ),( Da Jeong Kim ),( Ehn Kyoung Choi ),( Jin Tae Hong ),( Heon Sang Jeong ),( Hee Jung Kim ),( Su 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.6
The neuroprotective effects of a butanol fraction of white rose petal extract (WRPE-BF) were investigated in a middle cerebral artery occlusion (MCAO) model. Seven week-old male rats were orally administered WRPE-BF for 2 weeks and subjected to MCAO for 2 h, followed by reperfusion. Twenty-four h later, MCAO-induced behavioral dysfunctions were markedly improved in a dose-dependent manner by pretreatment with WRPE-BF. Moreover, higher dose of WRPE-BF not only decreased infarction area but also effectively reduced astrogliosis. The expression of inducible nitric oxide synthase, cyclooxygenase-2, and glial fi brillary acidic protein in MCAO model were markedly inhibited by WRPE-BF treatment. Notably, WRPE-BF decreased nitric oxide and malondialdehyde levels in the striatum and subventricular zone of stroke-challenged brains. These data suggested that WRPE-BF may exert its neuroprotective effects via anti-oxidative and anti-infl ammatory activities against ischemia-reperfusion brain injury and could be a good candidate as a therapeutic target for ischemic stroke.