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잔류 항균물질에 대한 미생물학적 간이검사법의 검출감도 비교
정승희,김진우,손상규,JUNG Sung Hee,KIM Jin Woo,SOHN Sang-Gyu 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3
본 연구는 EEC 4-plate법과 그 변법 그리고 식품공전상 간이검사법으로써 어류질병의 예방 및 치료에 사용되는 28종류의 항균물질에 대한 최저 검출한계를 서로 비교하여 어체내 잔류 항균물질의 최적 간이검사법 (bioassay)으로서의 유효성을 확인하고자 하였다. 식품공전상 간이검사법은 PCs에 뛰어난 검출감도를 나타내었으며 AGs에 대하여 좋은 감도를 보였으나 TCs, MLs, NFs, QNs에 대해서는 낮은 검출감도를 나타내었다. 한편, SAs에 대하여는 대단히 저조한 감도를 보였다. EEC 4-plate변법은 TCs에 대하여 우수한 검출감도를 나타내었다. EEC 4-plate법 및 그 변법은 PCs, MLs, NFs, QNs, SAs에 대하여 상대적으로 높은 검출감도를 나타내었다. 시험법들은 모두 CMs에 대하여는 검출감도가 낮았다. 결국 EEC 4-plate 변법이 여러계열의 항균물질에 대해 검출감도가 뛰어나고 항균활성의 범위가 넓어 어체내 잔류 항균성물질을 가장 유효하게 스크리닝할 수 있는 간이검사법으로 확인되었다. Minimun-detectable levels to 28 antibacterial agents used for the prevention and the treatment of fish diseases were determined to establish optimal detective method of bioassay in fish by the EEC 4-plate method, the modified method of EEC 4-plate and the standard method of analysis in food safety regulation. The test organisms used in the methods of bioassay were as follows: Bacillus subtilis BGA (B. subtilis) and Micrococcus luteus ATCC 9341 (M. luteus) in the EEC 4-plate method, B. subtilis, M. luteus and Bacillus cereus var. mycoides ATCC 11778 (B. cereus) in the modified of EEC 4-plate, and B. subtilis, M. luteus, B. cereus and Bacillus stearothermophilis var. calidolactis C-953 (B. stearothermophilis) in the standard method. The standard method showed predominant sensitivity in the detection of penicillins (PCs), and was also highly sensitive to aminoglycosides (AGs). The sensitivity of standard method in the detection of tetracyclines (TCs), marrolides (MLs), nitrofuran derivatives(NFs) and quinolones (QNs) was very low, and against sulfonamides (SAs), however, was extremely low. The modified method of EEC 4-plate showed very high sensitivity to TCs. Both the EEC 4-plate and the modified method of EEC 4-plate showed competitively high sensitivity in the detection of PCs, MLs, NFs, QNs and SAs. All the methods studied in the experiment showed very low sensitivity against chloramphenicol (CMs). Consequently, the modified method of EEC 4-Plate was the best bioassay method with a wide range of sensitivity for the optimal detection of the residual antibacterial agents in fish.
식물성 생약재 열수추출물이 어병 원인세균에 대한 항균활성 및 넙치 ( Paralichthys olivaceus ) 식세포의 활성산소 생산에 미치는 in vitro 효과
정승희(Sung Hee Jung),손영찬(Young Chan Sohn),김이청(Yi Cheong Kim) 한국어병학회 2001 한국어병학회지 Vol.14 No.1
The present study was to obtain a basic research data about medicinal herbs by screening in vitro antimicrobial activity and the production of superoxide anion(O₂^-) from the head kidney phagocytes of olive flounder, Paralichthys olivaceus. The following fourteen kinds of medicinal herbs extracted by boiling water were used : Gosam, Gwijeonu, Gujeolcho, Bagha, Bangpung, Yeongyo, Yagssug, Jiyu, Sambaegcho, Samjiguyeobcho, Sangbaegpi, Sohwehyang, Pyeonchug, Palgag. Antimicrobial activity against fish pathogenic bacteria, Listonella anguillarum, Vibrio sp., Vibrio alginolyticus, Edwardsiella tarda, Streptococcus sp. and Lactococcus garvieae, and the production of superoxide in kidney macrophage of olive flounder were examined by disk method and nitroblue tetrazolium(NBT) reaction, respectively. Among the tested herbs, Yagssug showed the highest antimicrobial activity against those fish pathogenic bacteria and stimulation of O₂^- production.
2007년∼2011년 하절기에 양식 넙치, 조피볼락, 참돔, 새우의 세균 및 기생충 감염 현황
정승희 ( Sung Hee Jung ),최혜승 ( Hye Sung Choi ),도정완 ( Jeung Wan Do ),김명석 ( Myoung Sug Kim ),권문경 ( Mun Gyeong Kwon ),서정수 ( Jung Soo Seo ),황지연 ( Jee Youn Hwang ),김석렬 ( Seok Ryel Kim ),조영록 ( Yeong Rok Cho ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Diagnostic monitoring in fish farms with land-based tanks and netpen cases were conducted in eastern, western, southern and Jeju island of Korea during summer of 2007∼2011. In total, 2413-fish samples of 4 marine fish species were tested for the detection of bacteria and parasite. Fish species tested were olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), red sea bream (Pagrus major), pacific white shrimp (Litopenaeus vannamei). During the diagnostic monitoring from 2007 to 2011, the infection rates by single infection of bacterial or parasitic pathogens were relatively higher than the mixed infections. The main bacterial pathogens in olive flounder, black rockfish and pacific white shrimp were Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.). The main bacterial pathogens in red sea bream were also Vibrio sp. and Photobacterium damselae subsp. damselae. The main parasitic pathogens were both Miamiensis avidus and Trichodina sp. in olive flounder, Microcotyle sebastes in black rockfish, Microcotyle tai in red sea bream and Zoothamnium sp. in pacific white shrimp.
Ampicillin의 경구, 주사 및 약욕 투약에 따른 넙치 혈액에서의 잔류량 변화
정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),박명애 ( Myoung Ae Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
The residue levels of ampicillin (AM) in cultured olive flounder, Paralichthys olivaceus (average 300g) at 20±1.0℃ were studied by oral, intramuscular and dipping administration (routes). The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01∼10 ppm were ranging from 84.45% to 91.26% for plasma. The limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 ㎎/㎏body weight), intramuscular injection (5, 10 and 20 ㎎/㎏body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-administration. In oral dosage of 10, 20 and 40 ㎎/㎏, it`s peak concentrations were 3.62±0.97, 5.20±0.70 and 11.18±0.87 ㎍/㎖, respectively at 10 h post-administration, but AM was not measurable at 144, 360 and 360 h post-administration, respectively. In intramuscular injection of 5, 10 and 20 ㎎/㎏, it`s peak concentrations were 6.92±1.29 ㎍/㎖, 9.89±2.22 ㎍/㎖and 19.85±2.97 ㎍/㎖, respectively at 5 h post-administration, but AM was not measurable at 216, 264 and 264 h post-administration, respectively. In dipping of 10, 20 and 40 ppm, it`s peak concentrations were 4.39±1.10, 9.57±1.51 and 11.61±1.92 ㎍/㎖, respectively at 3 h post-administration, but AM was not measurable at 264, 264 and 360 h post-administration, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.
조피볼락, Sebastes schlegeli에 경구투여된 nalidixic acid 및 piromidic acid의 약물동태에 미치는 수온의 영향
정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ),김진우 ( Jin Woo Kim ),박명애 ( Myoung Ae Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1
Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of nalidixic acid (NA) and piromidic acid (PA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of NA and PA were determined using HPLC-UV detector after a single dosage of 60 ㎎/㎏body weight. At 23±1.5℃, the peak serum concentrations of NA and PA, which attained at 24 h post-dose, were 5.87 and 0.43 ㎍/㎖, respectively. At 13±1.5℃, the peak serum concentrations of NA and PA, which attained at 10 h post-dose, were 6.22 and 1.57 ㎍/㎖, respectively. Better absorption of PA was noted at 13±1.5℃ compared to 23±1.5℃. However, absorption of NA was not affected significantly by temperature. The elimination of NA and PA from serum of black rockfish was considerably faster at 23±1.5℃ than at 13±1.5℃. The kinetic profile of absorption, distribution and elimination of NA and PA in serum were analyzed by fitting to a one compartment model, with WinNonlin program. The AUC, T1/2, Tmax and Cmax, respectively, were: 161.25 ㎍·h/㎖, 0.15 h, 12.29 h and 8.91 ㎍/㎖at 23±1.5℃, and 134.12 ㎍·h/㎖, 0.18 h, 8.79 h and 5.00 ㎍/㎖at 13±1.5℃ with NA; 41.57 ㎍·h/㎖, 0.58 h, 8.24 h and 0.21 ㎍/㎖at 23±1.5℃, and 40.36 ㎍·h/㎖, 0.59 h, 5.04 h and 1.20 ㎍/㎖at 13±1.5℃ with PA.
Thiamphenicol의 단일 경구투여에 따른 넙치와 조피볼락의 혈액내 잔류 분석
정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.3
This study was aimed at investigating the residual patterns of thiamphenicol (TP) in the cultured olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes schlegeli) following oral administration. TP concentration were detected by high performance liquid chromatography with UV detector. The recovery rates of TP in serums ranged 77.05~97.23% (olive flounder) and 89.96~97.11% (black rockfish) for the concentration of 0.1, 1.0, 10 ㎍/mL, respectively. After single administration of TP (100 ㎎/㎏ body weight) by oral route in olive flounder (700±50 g, 23±1.5℃) and black rockfish (500±30 g, 23±1.5℃), the concentration in the serum was determined at 1, 5, 10, 15, 24, 30, 50, 168, 264 and 432 h post-dose. Two-compartment model was applied to analyze in the pharmacokinetics of TP administered to the fishes. In the serum of olive flounder, TP was detected on 10 and 15 hours after treatment were 10.08 ㎍/mL and 10.06 ㎍/mL as maximum level, respectively. In the serum of black rockfish, TP was detected on 15 hours after treatment were 8.88 ㎍/mL as maximum level. Concentrations of TP to the fishes were not measurable at 432 hours (18 days) after treatment in all samples. Similar residual patterns of TP were revealed between the fishes. These results are helpful for estimating withdrawal time of TP which has been already in use for farmed fish treatment.
Florfenicol의 어류 병원성 세균에 대한 항균 효과와 경구 투여 후 넙치 혈액에서의 약물동태학적 특성
정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO) 한국수산해양교육학회 2013 水産海洋敎育硏究 Vol.25 No.5
The pharmacokinetics of florfenicol (FF) after oral administration was studied in the cultured olive flounder, Paralichthys olivaceus, using high performance liquid chromatography (HPLC). After single administration of FF (20 ㎎/㎏ body weight) by oral route in olive flounder (700±50 g, 23±1.5℃), the concentration in the serum was determined at 1, 5, 10, 15, 24, 30, 50 and 168 h post-dose. The kinetic profile of absorption, distribution and elimination of FF in serum were analyzed fitting to a two-compartment model by WinNonlin program. The area under the concentration-time curve (AUC), maximum concentration (Cmax), time for maximum concentration (Tmax) and elimination time were 22.51 μ g h/mL, 0.84 g/mL, 8.62 h and 447 h, respectively. The results of this study related to dosage and withdrawal times could be used for prescription of FF in field for the treatment of bacterial diseases in olive flounder.
사육방법 및 크기에 따른 조피볼락의 혈청 화학성분 변화
정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO),최혜승(Hye-Sung CHOI) 한국수산해양교육학회 2014 水産海洋敎育硏究 Vol.26 No.4
The purpose of this study was to obtain reference values of serum chemistry in Korean rockfish (Sebastes schlegeli), 5 juvenile (n=25) and 3 broodstock (n=15) subpopulations which were differentiated by cultured method. Serum samples were analyzed for the following commercial clinical kits: total protein, albumin, glucose, AST (aspartate aminotransferase), ALT (alanine aminotransferase), total cholesterol, free cholesterol, LDH (lactate dehydrogenase) and ALP (alkaline phosphatase). Significant differences were observed in the values of total protein, albumin, total cholesterol, AST, ALT, LDH and ALP between juvenile and broodstock subpopulations. In the comparison of total protein, albumin, total cholesterol and ALP, broodstock subpopulations showed higher levels. On the other hand, increased AST, ALT and LDH were observed in juvenile subpopulations. These results indicate that cultured method are major contributors to health-related physiology of Korean rockfish, juveniles and broodstocks.