骨格筋 Protein Methylase Ⅱ에 關한 硏究

洪承元,郭相太,林圭,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

Protein methylase Ⅱ (S-adenosy-L-methionine : protein-carboxyl 0-methyltransferase) has been, purifed 6,600-fold from bovine skeletal muscle and characterized. The purification procedures involved ammonium sulfate fractionation, Sephadex G-75 chromatography, S-adenosyl-L-homocysteine-Sepharose 4B chromatography, and hydroxyapatite chromatography. The enzyme showed an optimal pH around 6.0. It was heat-labile, being inactivated by heattreatment at 60℃ for 6 minutes. However, storage of the enzyme at -20℃ in the presence of 10% glycerol prevented the activity loss for 6 months. The apparent Km for S-adenosyl-L-methionine was 2.86 × 10 exp (6) M. Copper(Cu^2+) ion was potent noncompetitive inhibitor, the activity being completely inhibited b) 0.25 mM Cu^2+ and 1 mM Zn^2+. Most of the activity loss by copper and zinc ions were recovered by the addition of ethylenediamine tetracetate. The enzyme was also completely inhibited by 0.5 mM phydroxymercuribenzoate, which could be reversed by the addition of 10 mM 2-mercaptoethanol. The molecular weight of the enzyme was 24,000, as determined by Sephadex G-75 chromatography. Histone IIA, immunoglobulin A and soybean trypsin inhibitor were efficiently carboxylmethylated by the enzyme. Sarcoplasmic reticulum(SR) and sarcolemma(SL) of the skeletal muscle were also efficiently carboxylmethylated by the enzyme at a rate of 0.96 and 0.63 pmolesl mg protein/min of 3^H-CH_3, incorporation, respectively. Polyacrylamide gel electrophoresis of the carboxylmethylated SR and SL revealed multiple carboxylmethylated subfactions : SR resolved into the subfractions with molecular weight 100,000, 55,000, 22,000 and 14, 400, and SL into the subfractions with molecular weight 48,000, 22,000 and- 14, 400. Of these subfractions those with Mr=22, 000 were most strongly carboxylmethylated. Endogenous cAMP-dependent phoshorylation of SR occurred in the subfractions with Mr=55, 000, Mr=27, 000 and Mr=~14, 400, of which the fraction with Mr=27, 000 was most strongly phosphorylated. When SR was carboxylmethylated, the endogenous cAMP-dependent phosphorylation was decreased. SR exhibited relatively high Ca^2+-ATPase activity and oxalate-facilitated ATP-dependent 45^Ca^z+uptake. The carboxylmethylation and cAMP-dependent phosphorylation of SR increased the Ca^2+TATPase activity by 15% and by 29%, respectively. However, cAMP-dependent phosphorylation of SR which had been previously carboxylmethylated led to only 23% increase in the Ca^2+-ATPase activity. Both the carboxylmethylation and cAMP-dependent phosphorylation enhanced the steady-state rate as well as the initial rate of ^45Ca^2+-uptake. Kinetic analysis showed that the apparent Km and Vmax of Ca^2+ for ATP-dependent Ca^2+-uptake were 45μM and 35 nmoles/mg protein, respectively. Both the carboxylmethylation and cAMP-dependent phosphorylation of SR decreased the Km and increased the Vmax for the Ca^2+-uptake. These results suggest that protein methylase II might have some regulatory role in the calcium transport of sarcoplasmic reticulum.

Phytochrome이 보리잎 Protoplast의 팽윤에 미치는 영향

홍경애,송성준,송필순,유장걸,문두길 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-

암조건에서 생육시킨 보리(Hordeum valgare)잎의 생장점 부근에서 분리한 Protoplast가 적색광 처리에 의해서 팽윤되었고 적외선광에 의해서는 적색광 효과과 소멸되었다. 이 사실은 Protoplast의 팽윤이 Phytochrome에 의해서 조절됨을 시사하는 것이나 Phytochrome의 작용기작을 알기 위해서는 다각적인 연구가 필요하다. 실험결과로 부터 적색광 처리시간은 2분 적색광 처리후 배양시간은 2시간이 적당함을 알았다. Protoplast 배양액의 Sorbitol 농도에 따른 크기변화는 농도가 낮을수록 커졌으며 0.4M 이하에서는 Protoplast가 파괴되었다. Light effect on the size of protoplasts isolated from etiolated barley leaves was studied. Red light irradiation caused a swelling of protoplasts while little change in size found under the dark condition. Photoreversibility by red light and far-red light was found in protoplast swelling, indicating the involvement of phytochrome. Action mechanism of phytochrome on protoplast swelling should be clarified in aspect of growth regulators and intermediates involved. Two minute irradiation time and two hour incubation after red light treatment were found sufficient to induce protoplast swelling. The protoplasts were swollen according to the sorbitol concentration of incubation media but destroyed below 0.4M-sorbitol concentration.

새로운 重合體 溶液에서 Laser 光散亂 實驗

洪性樂,金斗哲 濟州大學校 基礎科學硏究所 1988 基礎科學硏究 Vol.1 No.1

연구 대상인 중합체 분자에 염(??, ??, ??)을 일정한 비로 혼합하여 묽인 용액에 대한 광산란 실험결과로 부터 1) 산란각이 작은 영역에서 산출된 회전반경(??)이 농도가 묽어질수록, 그리고 염의 종류에 따라서는 ??>??>??순으로 증가하였다. 이는 Han이 측정한 환산점성도의 결과와 일치한다. 2) 산란각이 큰 영역에서 ??의 함수와 ??함수를 비교할 때 농도가 짙은 상태에서는 분자 모형이 코일형이며, 농도가 묽어짐에 따라 막대형으로 변하고 있음을 추정할 수 있다. 3) 광산란 실험 결과로 산출된 환산점성도 값(??)이 점성도계로 측정한 값(??)과 서로 잘 일치하며 농도가 묽어짐에 따라 분자의 축비율이 증가함을 알 수 있다. 이로부터 농도가 묽어짐에 따라 분자모양이 막대형으로 변하고 따라서 회전반경(??)이 커지고, 그 결과로서 환산 점성도가 증가된다고 볼 수 있다. A measurements of reduced viscosity and light scattering study on new polyamides solution containing crown ether moieties were made in the presence of a salt(??, ??, ??). The result was that the reduced viscosity of solution increased rapidly as the concentration of solution decreased even if it was different as a sort of salt. In study of light scattering experiment, the angular dependent intensities of scattered light from each solution were measured. The Rayleigh ratios calculated from these data suggested that a change in molecular shape from coil to rod was responsible for the increased in the reduced viscosity of the polymar solution with dilution. The reduced visosity calculated from light scattering data agreed well with those measured with a viscometer.

식품저장수명에 대한 ceramic film package의 효과

홍상필,오두환,양융,선보경 연세대학교 산업기술연구소 1993 논문집 Vol.25 No.1

The positive of effects of ceramic film package on the shelf life of foods were compared with those of P.E. film. compared with P.E. film package, ceramic film package inhibited growth of microorganisms and decreased lipid oxidation velocity. Ceramic film package extended the shelf life of Vit. C and stabilized chlorophyll pigment. Total score in the organoleptic tests for ceramic film packed foods was higher than that for P.E. film packed foods.

퍼지논리 및 다중신호를 이용한 화재감지시스템의 개발

홍성호,김두현 한국안전학회 2004 한국안전학회지 Vol.19 No.1

This study presents an analysis of comparison of P-type fire detection system with system fuzzy logic-applied fire detection system. The fuzzy logic-applied fire detection system has input variables obtained by fire experiment of small scale with K-type temperature sensor and optical smoke sensor. And the antecedent part of fuzzy rules consists of temperature and smoke density, and the consequent part consists of fire probability. Also triangular fuzzy membership function is used for input variables and fuzzy rules. To calculate the final fire probability a centroid method is introduced. A fire experiment is conducted with controlling wood crib layer, cigarette to simulate actual fire and false alarm situation. The results show that peak fire probability is 25[%] for non-fire and is more than 80[%] for fire situation, respectively. The fuzzy logic-applied fire detection system suggested here is able to distinguish fire situation and non-fire situation very precisely.

새로운 중합체 용액에서 Laser 광산란 실험

홍성락,김두철 제주대학교 1986 논문집 Vol.23 No.-

A measurements of reduced viscosity and light scattering study on new polyamides solution containing crown ether moieties were made in the presence of a salt(K^(+).Cs^(+). Rb^(+)). The result was that the reduced viscosity of solution increased rapidly as the concentration of solution decreased even if it was different as a sort of salt. In study of light scattering experiment. the angular dependent intensities of scattered light from each solution were measured. The Rayleigh ratios calculated from these data suggested that a change in molecular shape from coil to rod was responsible for the increasein the reduced viscosity of the polymar solution with dilution The reduced visosity calculated from light scattering data agreed well with those measured with a viscometer.

Cellulase생산을 위한 Verticillium sp.의 배양조건의 검토

홍대희,진효상,오두환,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The cultivation conditions on wheat bran of verticillium sp. were optimized to produce cellulase as much as possible. The highest cellulase activity was shown, as verticillium sp. was cultivated at 27℃ for 5 days on wheat bran media with 1.2㎖/g wheat bran of water content and supplementation of NaNo and FeSo, in concentration of 0.1% and 0.2-0.3%, respectively.

폐경 전후 여성의 식이섭취가 골 밀도에 미치는 여향

홍성철,김문두,김성엽 제주대학교 생명과학연구소 2003 제주생명과학연구 Vol.6 No.1

Objective : To evaluate degree of bone mineral density and prevalence rate of osteoporosis according to age and to search the preventive strategy for osteoporosis, the factors that affect on bone density were studied in pre- and postmenopausal women in korea. Methods : The subjects were 961 premenopausal and 1,167 postmenopausal women who have examined in health promotion center of Yeungnam University Hospital in Daegu city from January, 1999 to January, 2001. The diet pattern of subjectives was analysed through interview with questionnaire. Results : The bone mineral density was statistically higher in women who have taken more calorie, protein, fat, or carbohydrate than recommended amount compared with women who have taken less calorie, protein, fat, or carbohydrate, than recommended amount in pre- and postmenopausal. In the effects of mineral and vitamin intake on bone mineral density, the higher intake of vitamin B12 and niacin increased bone mineral density statistically significantly in postmenopausal women, the women who have taken calcium more than 500 mg/day were revealed increased bone mineral density compared with less intake women in pre- and postmenopausal, and the women who have taken vitamin C more than 100 mg/day were revealed increased bone mineral density compared with less intake women in pre- and postmenopausal. Conclusion : These results suggested that to prevent osteoporosis, the systemized program including balanced diet for promoting anti-osteoporotic factors have to be developed and apply from the thirties whom have maximal bone mineral density.

ZnSe-ZnS : Mn 직류 박막형 전자발광 소자의 제작 및 특성조사

洪性樂,崔致圭,金斗哲,玄東杰 제주대학교 1988 논문집 Vol.27 No.-

A ZnSe-ZnS : Mn dc thin flim electroluminescene device was fabricated electron beam evaporation method and investigated the charcteristics. The brightness-voltage characteristics for dc operation were measured . The threshold voltage was 32V and at 60V the maximum brightness was 95fL. The brightness depending on the layer thickness of thin flim increased and a brightness tended to saturate above 8000A and the optimum temperature of substrate was 200-230℃. The spectrum of this device had a maximum intenty at wavelength 585nm.

자가 Fibrin Glue와 연골세포를 이용한 연골조직 공학 기법에 관한 연구

홍성표,범진식,박재경,김진영,이두형 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Neomorphogenesis of cartilage using chondrocyte-polymer constructs is a potential source for development of cartilage reconstruction. Current tissue engineering techniques of neocartilage rely on in vivo implantation of polymer-chondrocyte constructs. The purpose of this study was to find a way to bioengineer cartilage in vitro by entrapping chondrocytes in a molded autologous fibrin glue. Chondrocytes isolated from the cartilage of rabbit joints were combined with fibrinogen extracted by a single cryoprecipitation of autologous plasma, and they were then polymerized with thrombin to create a fibrin glue with a final cell density of 2.5x10?? cells/ml. The collagen for a control study was used as a polymer. The polymer-chondrocyte constructs were cultured for 4 weeks and the fibrin-chondrocyte constructs molded in the shape of a human ear were cultured for 6 weeks in vitro. Morphometric, histochemical, and histomorphometric analysis including glycosaminoglycan quantitation confirmed the following results: 1) Highly-concentrated autologous fibrinogen was easily extracted by a single cryoprecipition of autologous olasma. 2) The fibrin-chondrocyte constructs demonstrated the presence of actively proliferating chondrocytes with the production of cartilaginous matrix(collagen and glycosaminoglycan) at 1 week after culture, as well as gross and histologic evidence similar to those of normal cartilage at 3∼4 weeks after culture. 3) The collagen-chondrocyte constructs demonstrated lower degrees of hardness and transparency, as well as a lower density of cells and glycosaminoglycan during the culture period. 4) Neocartilage generated from fibrin-chondrocyte constructs in the shape of a human ear nearly retained their original configuration and size without degeneration for 6 weeks of culture in vitro. This study demonstrated a novel method for bioengineering the molded cartilage in vitro using autologous fibrin glue as a matrix scaffold. The generated cartilage showed gross and histologic evidence similar to those of normal cartilage, retaining the original gross dimension. With further refinement, this may be a new application of tissue engineering for the reconstruction of cartilage.