외과적 보조의 급속상악확장술 : 치험 5예 REPORT OF 5 CASES

박충열,이용욱,송종운,김영운,박홍주,오희균,유선열,이기현,황현식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Five adults (3 cases of bilateral posterior crossbite, 2 cases of unilateral posterior crossbite) with intermaxillary discrepancy of over 5㎜ due to maxillary transverse deficiency were treated by surgically assisted rapid maxillary expansion (SARME). Bilateral maxillary horizontal osteotomies, osteotomies of pterygomaxillary junction and anterior lateral nasal wall, ans anterior palatal osteotomy were performed in 4 cases, and unilateral osteotomies in 1case, followed by palatal expansion with tooth-borne orthopedic applicance. No significant complication was observed. The mean palatal expansion was 7.2㎜ (5.8∼10.0㎜) in the maxillary first molar region. During the mean follow-up period of 4 years (5 months∼8 years 7 months), no recurrence of crossbite was observed in all cases. The SARME seemed to be a simple, stable, and reliable procedure for achieving a permanent increase of over 5㎜ in skeletal maxillary transverse width of adults.

Validating of the pre-clinical mouse model for metastatic breast cancer to the mandible

HWANG, Young Sun,HAN, Sang-Sun,KIM, Ki-Rim,Ye-Jin, LEE,Sun-Kyung, LEE,Kwang-Kyun, PARK,Won-Yoon, CHUNG Faculdade de Odontologia de Bauru da Universidade 2015 Journal of applied oral science Vol.23 No.1

<P>Metastatic breast carcinoma has a great tendency to spread to the mandible. It is concomitantly associated with bone destruction, food intake disorder, and a poorer prognosis. Appropriate animal models need to be developed for a better understanding of the mechanisms underlying the metastatic process of breast cancer cells to mandible and to test the effects of potential lead compounds. Here, we assessed the metastasis model of intracardiac injection using luciferase-transfected metastatic breast cancer cells (MDA-MB-231<SUP>Luc+</SUP>) by determining the incidences of metastasis, mCT images, and histopathological results. A high bioluminescence signal mainly detected mandibular lesions with less frequent distal femora and proximal tibiae lesions. Extensive mandibular bone destruction occurred in nude mice grafted with metastatic breast cancer cells. This type of animal model might be a useful tool in assessing therapeutic implications and the efficacy of anti-cancer drugs for osteolytic cancers.</P>

김치로부터 분리된 Leuconostoc sp. strain YSK 균주에 의한 덱스트란 생산 조건의 최적화

황승균(Seung Kyun Hwang),홍준택(Jun Taek Hong),정경환(Kyung Hwan Jung),장병철(Byung Chul Chang),황경숙(Kyung Suk Hwang),신정희(Jung Hee Shin),임성팔(Sung Paal Yim),유선균(Sun Kyun Yoo) 한국생명과학회 2008 생명과학회지 Vol.18 No.10

본 연구는 발효 김치 액으로부터 덱스트란 생산 균주를 분리하고 생산 최적 생산 조건을 설정하기 위하여 반응표면분석법을 이용하였다. 발효 조건의 독립변수들은 배양 온도, pH, 효모 추출물의 농도, 온도, 기질의 농도로 정하고 Box-Benken 디자인를 이용하여 실험을 설계하였다. 최종 분리된 균주는 Leuconost℃ sp. strain SKY로 잠정적으로 명하였다. 연구 결과 덱스트란 생산은 3.90 - 22.40 g/l이고, 균체량 생산은 0.69-2.85 g/l, 수율은 0.10-0.64, 생산 속도 0.16-0.85 g/l-hr의 범위에서 분석이 되었다. 표면반응분석 결과 덱스트란 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 균체량 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 생산 수율에 가장 영향을 미치는 것은 배양 pH 이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 덱스트란 생산 속도에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 결론적으로 최적 생산 조건은 온도는 27-28℃이고, pH는 7.0이며, 효묘 추출물은 6-7%의 범위에서 결정이 되었다. 이러한 조건에서 생산된 덱스트란양은 22g/l이고, 생산 수율은 약 60%정도이며, 생산 속도는 0.8g/l/hr이었다. A bacterium producing non- or partially digestible dextran was isolated from kimchi broth by enrichment culture technique. The bacterium was identified tentatively as Leuconostoc sp. strain SKY. We established the response surface methodology (Box-Behnken design) to optimize the principle parameters such as culture pH, temperature, and yeast extract concentration for maximizing production of dextran. The ranges of parameters were determined based on prior screening works done at our laboratory and accordingly chosen as 5.5, 6.5, and 7.5 for pH, 25, 30, and 35℃ for temperature, and 1, 5, and 9 g/l yeast extract. Initial concentration of sucrose was 100 g/l. The mineral medium consisted of 3.0 g KH₂PO₄, 0.01 g FeSO₄ㆍH₂O, 0.01 g MnSO₄ㆍ4H₂O, 0.2 g MgSO₄ㆍ7H₂O, 0.01 g NaCl, and 0.05 g CaCO₃ per 1 liter deionized water. The optimum values of pH and temperature, and yeast extract concentration were obtained at pH (around 7.0), temperature (27 to 28℃), and yeast extract (6 to 7 g/l). The best dextran yield was 60% (dextran/g sucrose). The best dextran productivity was 0.8 g/h-l.

저 전압스트레스 및 다채널 전류평형을 위한 Floating 전압 스택형 단일스위치 LED 구동회로

황원선(Won-sun Hwang),류동균(Dong-kyun Ryu),최흥균(Heung-kyun Choi),김희욱(Hugh Kim),한상규(Sang-kyoo Han) 전력전자학회 2014 전력전자학술대회 논문집 Vol.2014 No.7

Comparison of Cytokine Expression in Mesenchymal Stem Cells from Human Placenta, Cord Blood, and Bone Marrow

Hwang, Jong Ha,Shim, Soung Shin,Seok, Oye Sun,Lee, Hang Young,Woo, Sang Kyu,Kim, Bong Hui,Song, Hae Ryong,Lee, Jae Kwan,Park, Yong Kyun The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.4

<P>Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROα(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.</P>

만성담마진환자(慢性蕁麻疹患者)의 단자시험성적에 관한 연구

황선욱,김형균,임춘평 대한피부과학회 1979 大韓皮膚科學會誌 Vol.17 No.4

In a large proportion of patients with chronic urticaria, at present, the definite tiology can't be found. There have been occasional attempts to identify the aIIergic causes uaing allergic skin tests, only ta suggest the Candicla albicans and some food yeasts ae probabIe causes. This atady was designed to investgate how often patients with chronic urticaria from Chonnam Province react positively to skin tests with extracts of 42 common. allergene in the standard group Al-13 produced by Bencard Ltd. in England. The authora alao surveyed the clinical characteristics of 63 patients with chronic urtimria of more than 3 months duration, who bad visited the skin clinic of Chosun Univeresity HcepitaI from May. 1977 throug 3. Apr. 1979. The results were summarized as follows; 1. Among 63 patients, the maIe to female ratio was 1: l.2, and the peak incidence was in the 20s and 30s. 2. In regard to the duration of chroic urticaria, the highst frequency waa below one year duration. 3. Among 60% of the pa,tients who experienced seasonal changes, 32.4% had suffered aggravation during the winter. 4. The most common diseases combined with chronic urticaria were allergic rhinitis and atopic dermatitis, occuring in 9.5% of all subjects. 5. Of thase examined, 69.8% showed positive reactions to the prick test with the peak incidence in the 20s and 30s. 6. Among 42 allergens used far the prick test, 28 allergens turned out to be possibly casal agents, the rnost frequent group of allergens involved were grain du sts (27.1%), animal hairs(21.8%) and moulds(16.5%) in that order of frequency and occupying over 65% of the total positive reactions. And the most frequently involved specific allergens were hay dust, mixed grain dust straw dust and tree in that order of frequency. Moreover, 90 of the involved allergens were inhalants. 7. In teen-agers, the reactions to grain dusts, animal ha.irs, rnoulds and pollens were the rnost frequent while reactions to food allergens were most frequent in the 20s, and cotton and house dust are most frequently involved in tbe 40s. 8. Multiple sensitivity was not:d in a majority of cases and the most frequent combination was 2 to 5 allergens. 9. The rnost common abnormal hematologic finding in chronic urticaria was eosinophilia.

Preparation of $ZrO_2-CaO$ fiber by using a chemical solution process

Hwang, Kyu-Seog,Jeon, Young-Sun,Kim, Sang-Bok,Kim, Chi-Kyun,Oh, Jeong-Sun,An, Jun-Hyung,Kim, Byung-Hoon The Korea Association of Crystal Growth 2003 韓國結晶成長學會誌 Vol.13 No.6

In this work, chemical solution derived Ca-doped zirconia fiber has been prepared by using calcium- and zirconium-naphthenate. Fibrous $ZrO_2$-CaO was drawn from a sticky mixture. Dried gel fibers were finally annealed at $1000^{\circ}C$ for 1 h in argon. 91 mol%$ZrO_2$-9 mol%CaO fiber consisted of tetragonal, monoclinic and $CaZrO_3$ phases after annealing at $1000^{\circ}C$. On the other hand, samples annealed at $500^{\circ}C$ consisted of almost tetragonal single phase. Homogeneous fibers surface at $500^{\circ}C$ became rougher after $1000^{\circ}C$-annealing. The sample annealed at $1000^{\circ}C$ with relatively rough surface structure showed a high Calcium phocphate forming ability.

Functional invadopodia formation through stabilization of the PDPN transcript by IMP-3 and cancer-stromal crosstalk for PDPN expression.

Hwang, Young Sun,Xianglan, Zhang,Park, Kwang-Kyun,Chung, Won-Yoon IRL Press] ; Oxford University Press 2012 Carcinogenesis Vol.33 No.11

<P>We previously reported that insulin-like growth factor-II mRNA-binding protein-3 (IMP-3) depletion (IMP-3(δ)) was shown to inhibit invadopodia formation and extracellular matrix degradation capacity in oral squamous cell carcinoma (OSCC) cells. In this study, we found that IMP-3(δ) cells significantly downregulated the podoplanin (PDPN) level, which resulted in a loss of extracellular matrix degradation activity, although invadopodia was still thriving. From RNA in situ hybridization using a digoxigenin-labeled 3'UTR recognition probe of PDPN and reporter assay with 3'UTR of the PDPN gene cloned downstream from the luciferase reporter gene, we revealed that IMP-3 depletion was shown to be downregulated, which most probably lowered PDPN gene expression by reducing mRNA stabilization. In a xenograft model, PDPN depletion was the cause of a decrease in tumor volume and regional infiltration into nearby stroma. Taken together, transforming growth factor beta 1 increased PDPN expression, which potentiated cancer invasion through increased invadopodia formation and extracellular matrix degradation in the low invasive OSCC cell line. Reciprocally, interleukin-1 beta secreted by OSCC cells, stimulated transforming growth factor beta 1 secretion from stromal fibroblasts to induce PDPN expression in OSCC cells. In addition, a retrospective investigation of OSCC patients found that IMP-3 and PDPN expression significantly correlated with lymph node metastasis of OSCC patients. Moreover, co-expression of IMP-3 and PDPN were frequently detected both in primary and lymph nodes metastatic OSCC cells using immunohistochemical dual staining. Thus, the IMP-3-PDPN axis may be a sensitive target molecule in anti-invadopodia therapy for the treatment of metastatic cancers.</P>

Changes in prevalence and perception of complementary and alternative medicine use in Korean inflammatory bowel disease patients: results of an 8-year follow-up survey

( Sun-ho Lee ),( Kiju Chang ),( Ki Seok Seo ),( Yun Kyung Cho ),( Eun Mi Song ),( Sung Wook Hwang ),( Dong-hoon Yang ),( Byong Duk Ye ),( Jeong-sik Byeon ),( Seung-jae Myung ),( Suk-kyun Yang ),( Sang 대한장연구학회 2020 Intestinal Research Vol.18 No.2

Background/Aims: The use of complementary and alternative medicine (CAM) is a global phenomenon, including inflammatory bowel disease (IBD) patients. We aimed to assess the change in prevalence and patterns of CAM use, and attitudes towards CAM over an 8-year time interval (2006 vs. 2014) among IBD patients in Korea. Methods: A total of 221 IBD patients (CD=142, UC=79) were asked to complete a questionnaire regarding CAM at two time points: at enrollment (2006) and 8 years later (2014/2015). Results: The proportion of patients ever using CAM increased significantly from 60.2% in 2006 to 79.6% in 2014 (P<0.001), while the proportion of current CAM users increased slightly (35.7% to 38.0%, P=0.635); 21.7% used CAM consistently at both time points. The proportion of patients who felt CAM was less effective (P<0.001) and more expensive (P=0.04) than conventional treatments increased over time. Also, the proportion among ever CAM users who perceived a positive effect from CAM significantly decreased in 2014 compared to 2006 (P=0.004). Higher education (adjusted odds ratio [aOR], 2.10), prior side effects to conventional therapies (aOR, 2.23), and prior use of corticosteroids (aOR, 2.51) were associated with CAM use. Interestingly, use of CAM before IBD diagnosis (aOR, 2.73) was significantly associated with consistent CAM use. Conclusions: Although the attitudes toward CAM have become less favorable, the majority of IBD patients have experienced CAM with an overall increase of current CAM users over time. Moreover, more than half of current CAM users used CAM consistently over time. (Intest Res 2020;18:192-199)

EPM 법에 의해 제조된 Ti-46.6Al-1.4Mn-2Mo 합금의 기공 제어

황선근,김종균 대한금속재료학회(대한금속학회) 1998 대한금속·재료학회지 Vol.36 No.9

Formation mechanism and control of pores in a gamma alloy synthesized by elemental powder metallurgy(EPM) was investigated. The chemical composition of the experimental alloy was Ti-46.6Al-1.4Mn-2Mo(at.%) and elemental powders were used as raw materials to synthesize the alloy by hot extrusion. Retained molybdenum was observed in as-extruded alloy, and formation of pores at molybdenum-rich regions was confirmed in heat treated alloy by back scattered electron imaging as well as by energy dispersive spectroscopy, The porosity increased with the average particle size of Mo powder. Depending on the processing condition the density of pores varied. Pores could not be easily detectable in as-extruded condition ; however, subsequent heat treatment brought them out. Pre-extrusion treatment affected the severity of porosity in extruded and heat-treated condition. Extended soaking or partial sintering prior to extrusion was found to be effective for suppressing porosity during the heat treatment to produce a fully lamellar structure.