호알칼리성 Bacillus sp. No. 4의 Cyclodextrin Glycosyltransferase에 의한 Glycosyl Sucrose의 생산과 저충치성 당으로서의 응용

손천배,유미경,김명희,문숙경 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Action of a cyclodextrin glycosyltransferase (CGTase) produce from alkalophilic Bacillus sp. No. 4 was studied in a solution containing starch and sucrose to prepare glycosyl sucrose syrup with good sweetness and antidecaying properties of teeth. In the initial stage of the reaction the CGTase produced cyclodextrin, however, the cyclodextrin disappeared and glycosyl sucrose was formed with the lapse of reaction time. The best proportion of sucrose to starch for prodution of glycosyl sucrose was about 1 : 1. The optimum pH and temperature of the coupling reaction was pH 6.0 and 60℃, respectively. Main composition of glycosyl sucrose syrup prepared with 20% starch and 20% sucrose was sucrose 18%, glucosyl sucrose (G_2F) 15.3% and amltosyl sucorse (G_3F) 11.3%. And glucose, maltose and maltotriose were produced very little. Smaller amounts of acid and insoluble glucan were formed in the syrup by Streptococcus mtans OMZ176 than in the sucrose. Therefore, the prepared glycosyl sucrose sucrose syrup is expected to prevent teeth from decaying.

지속적 및 간헐적 가압력이 치주인대 배양세포의 Alkaline Phosphatase 활성도에 미치는 영향

성재현,경희문,배성민,권숙이 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.4

지속적 및 간헐적인 가압력이 배양치주인대 세포의 ALP활성도에 미치는 영향을 알아보기 위하여 치주인대세포를 24 well 배양접시에 배양한 후 밀생상태가 되었을 때, 세포배양기 속의 특수제작된 사각상자에 배양접시를 넣은 후 실험기간동안 300g/㎠의 압력을 10분간 가압시킨 후 10분간 가압이 중지되도록 한 간헐적 가압군, 그리고 압력을 가하지 않은 군을 대조군으로 하여 각각 실험 24시간, 72시간 후의 alkaline phosphatase의 활성도를 측정하여 다음과 같은 결과를 얻었다. 1. 가압 24시간 군에서는 간헐적 가압군이 대조군에 비하여 ALP활성도가 낮게 나타났다(p>0.05). 2. 가압 48시간 군에서는 실험군은 대조군에 비하여 ALP활성도에 유의한 차이는 나타나지 않았다. 3. 가압 72시간 군에서는 지속적 가압군이 대조군에 비하여 ALP활성도가 높게 나타났다(p>0.01). The purpose of this study was to evaluate the effect of cellular activity on PDL cells dependent on intermittent and continuous compressive force by determining the alkaline phosphatase activity. An intermittent and continuous compressive force were applied on PDL cells at the confluent stage. The alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. The experimental group were consist of continuous and intermittent compressive group which were compressed by 300g/㎠ of diaphram pump. The intermittent compressive group was connected by timer which was worked on 10 minutes and off 10minutes. The results were as follows; 1. The alkaline Phosphatase activity of intermittent compressive group was lower than control at 24 hours(p>0.05). 2. The alkaline Phosphatase activity between each groups showed no significant differences at 48hours. 3. The alkaline Phosphatase activity of continuous compressive group was significantly higher than control group at 72 hours(p>0.01).

特用資源植物의 硏究 : 한국임학회지에 게재된 논문을 중심으로 Based on the Articles Published in the Journal of Korean Forestry

이재선,김철우,배찬호,송재모,강효진,황석인,문흥규 江原大學校 森林科學硏究所 2003 Journal of Forest Science Vol.19 No.-

한국임학회지 (1962년의 1호~2002년의 91권 6호)에 게재된 특용자원식물 관련 논문에서 식·음용식물, 약용식물, 사료식물, 조경식물, 섬유식물, 산업용식물 및 밀원식물로 분류하고 그 내용에 따라 입지환경, 생태, 생리, 번식, 조림(또는 무육, 재배), 유전·육종, 분류(또는 식별), 병·충해와 방제, 동물피해와 방제, 성분분석, 식생정보, 생물공학, 경영·경제 및 총설로 나누었다. 총 1,434편의 발표 논문 중 유용식물자원을 대상으로 한 논문은 27.6%를 차지하는 396편이었고, 내용은 식생정보 60편(15.2%), 생리 56편(14.1%), 유전·육종 56편(14.1%), 번식 53편(13.4%) 및 생태 37편(9.3%)이었다. 조림(무육·재배)은 11편(2.8%)으로 특용자원식물의 규모경영이 이루어지지 않고 있음을 시사하고 있으며, 이것은 경영·경제 분야가 6편(1.5%)인 것과 맥을 같이 하고 있었다. 연구대상 식물은 잣나무(42편), 아까시나무(23편), 밤나무(14편) 및 은행나무(14편) 순으로 높았고, 이 식물들과 관련한 연구내용은 번식, 생리, 유전·육종, 생태 및 병·충해 순이었다. 연구의 목적, 내용, 결과 및 대상식물 등을 고려하여 보면, 1) 초본식물에 대한 보다 많은 연구, 2) 타산업 또는 학분 분야와의 공동연구, 3) 자원 보전적 연구 및 4) 연구주체자 간의 연구결과의 공유 등이 매우 요망된다. The articles, published in the Journal of Korean Forestry from Number 1(1962) to number 6, Volume 91(2002), were surveyed and investigated for the research trend analysis about resource-plants for special use or purpose, i.e edible plants, medicinal plants, feed resource, landscape plants, fiber plants, industrial usage, and bee plants. If the purpose or subject matter of the research was construction or furniture timber production, mushrooms and/or pulp and Paper, such research was not included in this study. These articles were classified again depending on the content of research into 14 categories : habitat environment, ecology, physiology, propagation, silviculture(tending and culture), genetics and breeding, identification, insect and disease control, animal-related research, cemponent analysis, vegetation survey, biotechnology, management, and review. Among the total 1,434 articles published, 396 ones (27,6%) were related with plants for special use or purpose. Vegetation survey was 60(15.2%) ; physiology 56(04.1%) ; genetics and breeding 56(14.1%) ; propagation 53(13.4%) ; and ecology 37(9.3%). Siviculture research field included 11 articles (2.8%), which indicates that the management of resource-plants is to far from economic income as seen in the low number of management research filed articles, i. e., only 6 reports(1.5%). Korean white Pine was most popular for research and included 42 articles ; Robinia pseudoacacia 23 Castanea crenata 14 : and ginkgo tree 14, Research related with these species had focused mainly or propagation, physiotogy, genetics and breeding, ecology and pest control. Based on this survey and analysis, the followings are suggested : 1. More research is required on forest herbaceous plants. 2. Cooperative research work with other industrial and/or scientific area is recommendable for commercialization including medicine, cosmetics, and food etc. 3. Research resource-plant conservation. which includes biology, social education and policy, should be supported for next generation. 4. Mutual correspondence and information exchange about the research results between researchers and institutes is more necessary than now.

Migrating motor complex changes after side-to-side ileal bypass in mouse ileum ex-vivo

Suk-Bae Moon,Kyu-Joo Park,Jung-Sun Moon,Eun-Kyoung Choe,In-Suk So,Sung-Eun Jung 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.80 No.4

Purpose: This study was intended to investigate the migrating motor complex (MMC) changes after ileal bypass in ex-vivo mouse models. Methods: Partial (side-to-side) and total bypass (occlusion of proximal part of bypassed loop) were performed on ileums of female Institute of Cancer Research mice. After 2 and 4 weeks, the bypassed segments were harvested and MMCs were recorded at 4 different sites ex-vivo. Amplitude, duration, interval, direction of propagation, and the area under the curve (AUC) of MMCs were measured and compared to those of the controls. Results: In control mice (n = 7), most MMCs propagated aborally (91.1%). After 2 weeks of partial bypass (n = 4), there was a significant decrease in both amplitude and AUC, and orally-propagating MMCs increased significantly (45%, P = 0.002). Bidirectional MMCs (originating in the bypassed loop and propagating in both directions) were also observed (10%). The amplitude of the MMCs remained decreased at 4 weeks after partial bypass (n = 4), and neither the AUC nor the direction of propagation showed significant changes compared to 2 weeks. Similarly, in the total bypass model, both the amplitude and AUC of the MMCs decreased significantly compared to controls. In contrast to partial bypass, 95% of the MMCs within the bypassed loop propagated aborally after 2 weeks (n = 6), which was similar to the control state. After 4 weeks (n = 5), however, MMCs either lost their temporal relationship or completely disappeared. Conclusion: The changes in propagation direction of the MMCs in the partially bypassed loop may contribute to stagnation of bowel contents and the development of blind loop syndrome.

[Pediatric] Outcomes of Pediatric Appendicitis in the Era of COVID-19 Pandemic: Comparison Between before and during the Pandemic

Suk Bae Moon,Gibong Chae,Sang-Ji Choi,Sung-Bae Park,Seong Kweon Hong,Yang Hee Kim,Miyoung Jang,Hwansoo Kim 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

Hepatoblastoma

Suk-Bae Moon,Hyun-Baek Shin,Jeong-Meen Seo,Suk-Koo Lee 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.81 No.2

Purpose: Hepatoblastoma is the most common malignant liver tumor in children. The aim of this study was to review our results of hepatoblastoma treatment and to determine the role of surgical treatment in hepatoblastoma. Methods: This is a retrospective clinical study. The medical records of patients with hepatoblastoma, treated between October 1994 and October 2009, were reviewed. The patients were classified according to the pretreatment extent of disease (PRETEXT) grouping system. The main outcome variable was survival. Secondary outcome variables were complete, partial and no response to chemotherapy and surgery, when indicated. Results: Twenty-seven patients were treated during the observation period. Eighteen were males. Five were PRETEXT group I, 8 group II, 13 group III and 1 group IV. Complete excision was achieved in all patients except in one case that underwent liver transplantation (group IV). Median follow-up and survival rate were 2.3 years and 100%, 6.6 years and 75%, 5.8 years and 92%, 7.7 years and 100%, for groups I to IV, respectively. Twenty patients are currently considered to be in complete response status and three patients are receiving postoperative chemotherapy. Four patients died; the causes of death were cytomegalovirus hepatitis, bone marrow suppression during adjuvant chemotherapy, primarynonfunction after the transplantation for recurrent tumor and metachronous rectal cancer, respectively. Conclusion: Favorable long-term outcome could be expected for hepatoblastoma with complete tumor excision and adjuvant chemotherapy.

인간 면역결핍 바이러스 양성환자의 항문부 첨규 콘딜로마에서 관찰되는 인유두종 바이러스의 아형에 관한 연구

문석배(Suk-Bae Moon),문상희(Sang-Hee Moon),박규주(Kyu-Joo Park) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.2

Purpose: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray. Methods: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (M:F=15:2, mean age 35 years, 22∼59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip. Results: Fourteen patients (9 HIV (+), 5 HIV (?)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (?) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV. Conclusion: Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (?) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.

극소 저출생 체중아에서 SMOFlipid의 투여가 미숙아 질환 예방에 미치는 영향: Cholestasis 예방 중심으로

김문진 ( Moon Jin Kim ),배혜정 ( Hye Jung Bae ),조윤숙 ( Yoon Sook Cho ),김귀숙 ( Kwi Suk Kim ),김향숙 ( Hyang Suk Kim ),이혜숙 ( Hye Suk Lee ),이주영 ( Ju Young Lee ),김이경 ( Ee Kyung Kim ),김한석 ( Han Suk Kim ) 한국정맥경장영양학회 2013 한국정맥경장영양학회지 Vol.5 No.2

Purpose: Very low birth weight infants (VLBWI) are found to have a higher incidence of cholestasis due to long-term total parenteral nutrition (TPN). Although there have been some studies on relationships between omega-3 fatty acids and reduced incidence of cholestasis, the advantages that the fatty acids provide for premature infant diseases have not yet been fully understood. The aim of this study was to evaluate the advantages of SMOFlipid, a lipid emulsion product that contains omega-3 over other lipid emulsion products that do not. Methods: The medical records of 182 newborn (127 boys and 55 girls) were reviewed retrospectively between April 2010 and August 2012. These infants were born with a birth weight of <1,500 g and administered either of lipid emulsions (Intra MCT 20% or SMOFlipid) from birth to a full-feeding condition at our neonatal intensive care unit. Cholestasis is defined as serum direct bilirubin (DB) >1.0 mg/dL when total bilirubin (TB) is <5 mg/dL or DB/TB ratio is >20% when TB >5 mg/dL. Results: The incidence of cholestasis was found to be lower in the SMOFlipid group than in the medium-chain triglyceride/long chain triglyceride (MCT/LCT) group (7.3% [4/55] versus 18.9% [24/127], P=0.046). At the start of lipid emulsion administration (day 0), the baseline bilirubin level showed no significant difference between the two groups. However, the maximum value of DB on days 7∼ 14 was lower in the SMOFlipid group than in the MCT/ LCT group (0.8 [0.3∼2.2] versus 1.1 [0.3∼2.8] mg/dL, P= 0.030). The DB/TB ratio was also lower in the SMOFlipid group than in the MCT/LCT group (10.2% [4.9%∼40.0%] versus 24.1% [5.1%∼62.5%], P=0.002). Conclusion: This study concluded that SMOFlipid was effective in the prevention of cholestasis in VLBWI. Therefore, omega-3-containing lipid emulsion can be highly recommended in premature babies, especially in VLBWI who require TPN support. (JKSPEN 2013;5(2):67-75)

학술 7 특별구두세션 : F2. 학술세션 QM 적용: 국방 ; 국방 군수업체 품질경영 수준 평가 모델 개발

배석주 ( Suk Joo Bae ),정규석 ( Kyu Suk Chung ),류문찬 ( Moon Charn Riew ),임성욱 ( Sung Uk Lim ),김명준 ( Myung Joon Kim ),김성도 ( Sung Do Kim ),양지응 ( Ji Eung Yang ),박상호 ( Sang Ho Park ) 한국품질경영학회 2014 한국품질경영학회 학술대회 Vol.2014 No.2

1. 목적 · 기존 모델을 조사하고 분석하여 국내 국방 군수업체 품질경영 수준을 진단하고 평가하기 위한 모델 제시 2. 연구설계/ 방법론/ 접근방법 · 기존 ME, EQA, JQA 모델을 비롯하여 국내 모델들의 특징을 살펴보고 국내 실정에 맞는 정량 지표도출과 정성적 지표의 객관화 평가를 위한 표준척도 마련 3. 연구결과 · 국방 분야의 특징을 살피고 수준진단을 통해 정책제시 및 로드맵 작성 방안 제시 4. 실무적 시사점 · 국내 국방분야 특성을 반영한 모델 제시 및 객관화된 지표 마련 5. 독창성/ 가치 · 과정과 결괴를 따로 측정하는 방식에서 탈피하여 평가항목의 인과관계를 강화하고 러닝 개념을 도입하여 현재 상태뿐만 아닌 과거와 미래까지 트렌드를 파악하여 평가할 수 있는 모델 확장

요양보호사의 구강보건행태에 관한 연구

배성숙(Sung-Suk Bae),문하영(Ha-Young moon),문윤미(Yun-Mi Moon) 한국엔터테인먼트산업학회 2014 한국엔터테인먼트산업학회논문지 Vol.8 No.4

이 연구는 충청지역의 노인요양병원과 시설 11개소에서 근무하는 요양보호사 127명을 대상으로 요양보호사의 구강보건행태를 파악하고 행태에 영향을 미치는 요인을 분석함으로써 노인건강복지정책을 위한 구강보건교육프로그램 개발과 노인을 위한 구강건강증진사업 접근방법을 모색하고자 조사가 실시되었다. 1. 구강보건지식과 구강보건행태의 상관관계를 분석한 결과로서 양(+)의 관련성이 있고, 유의한 상관관계가 나타났다(p=.001). 2. 구강보건행태에 영향을 미치는 관련요인으로는 구강보건지식 p=.029, 구강건강교육경험 p=.000로 나타나 두 변수 모두 유의한 결과가 나타났다. 수정된 결정계수(Adj.R²)는 .250으로 나타났고, 분산분석 결과(f=22.001) p-v가 .000이므로 의미가 있음을 알 수 있었다. 이상을 종합해보면, 현대사회는 생활수준과 삶의 질향상으로 건강에 대한 관심이 높아지고 있으며 구강보건관리가 중요한 부분으로 인식되어 지고 있다. 특히 요양병원과 시설에서 근무하는 요양보호사들의 노인의 구강보건관리의 역할을 담당하고 있으므로 그들을 위한 구강보건교육프로그램 개발과 구강보건전문가들의 지도 및 관리가 필요할 것으로 사료된다. This study was conducted to develop an oral health education program for senior citizen"s health & welfare policy and search for an oral health enhancement program for senior citizens by understanding the oral healthcare behavior of care workers and analyzing the influencing factors of behavior with 127 care workers that are working at 11 convalescence hospitals for senior citizens and facilities in Chungcheong region. In the analysis of the correlation between oral health behavior and oral health knowledge, meaningful correlation was revealed with positive correlation(p=.001). In terms of related influencing factors on oral health behavior, meaningful results were shown for both variables with p=.029 for oral health knowledge and p=.000 for oral health education experience. To summarize, it would be necessary to develop an oral health education program for care workers working at convalescence hospitals and facilities playing the role of caring for the oral health of senior citizens with related guidance and supervision for oral health experts.