세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

차단기의 50/60㎐ 공용주파수 정격의 시험방법에 관한 검토

서윤택(Yoon-Taek Suh),박승재(Seung-Jae Park),이용한(Yong-Han Lee),김맹현(Maeng-Hyun Kim),윤학동(Hak-Dong Yun),김대원(Dae-Won Kim) 대한전기학회 2004 대한전기학회 학술대회 논문집 Vol.2004 No.7

3상 90MVA 단시간전류시험 설비 구축

서윤택(Yoon-Taek Suh),김용식(Yong-Sik Kim),윤학동(Hak-Dong Yun),김맹현(Maeng-Hyun Kim) 대한전기학회 2005 대한전기학회 학술대회 논문집 Vol.2005 No.7

퓨즈 부착 고압교류 부하개폐기의 국제규격(IEC62271-105) 개정에 관한 검토

서윤택(Yoon-Taek Suh),류형기(Hyeong-Kee Rhyou),김대원(Dae-Won Kim),H.D Yoon 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7

대전력시험에서 고전압 측정시스템의 불확도 평가

서윤택(Yoon-Taek Suh),김맹현(Maeng-Hyun Kim),김대원(Dae-Won Kim),정주영(Joo-Young Jeong),강영식(Young-Sik Kang) 대한전기학회 2002 대한전기학회 학술대회 논문집 Vol.2002 No.7

대전력시험의 새로운 측정시스템에 대한 불확도 평가

서윤택(Yoon-Taek Suh),김맹현(Maeng-Hyun Kim),김대원(Dae-Won Kim),강영식(Young-Sik Kang) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7

Gilles de la Tourette氏 病의 一例

徐光潤,朴魯澤 大韓神經精神醫學會 1969 신경정신의학 Vol.8 No.1

A clinical syndrome consisting of multiple tics was frist mentioned by Itard in 1825 and subsequently recorded in greater detail by Gilles de la Tourette. He defined it as a “nervous affliction characterized by motor incoordination accompanied by echolalia and coprolalia.” It has since become known by his name. In this paper, a case of Gilles de la Tourette's disease that was observed in a Korean boy, aged 14 was presented. The characteristic features of this case were as follows: 1) His illness began at the age of 9. Characteristic symptoms consisting of multiple tics of the face, shoulder and extremities accompanied with vocal tic were observed, and also the episodes of echolalia and coprolalia could be revealed by anamnestic history. 2) The family and developmental history revealed that the parental role was characterized by domineering, rejecting and castrating one, and the sibling relationship was also competitive and rival. In this family structures with obsessive feature during his early part of life. 3) In treatment, psychotherapy and the use of prop itan, a butyrophenone derivative brought to considerable effect. 4) From the genetic and dynamic view point, it was supposed that the overwhelming anxiety originated from frequently coerced separations from both of his parents since his early childhood, the castrating fear which was reinforced by his mother in later childhood and the mothering hostility and guilt fostered by these experiences were the most important factors. In his symptomatic dealing with these anxiety, hostility and guilt, it was also supposed to be that this boy deloped hysterical and obsessive-compulsive defenses simultaneously.

성기감염환자에서 Chlamydia Trachomatis 감염율에 관한조사

서영욱,윤성도,이태성,김택훈 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.1

Chlamydia trachomatis was known as one of the important etiologic ororganisms of pelvic inflammatory disease in women, which result in pelvic peritoneal and tubal pathology or infertility. This study was conducted to assess the incidence of chalmydia trachomatis in gynecologic patient. The results were as follows: The incidence of chamydial infection in gynecology paients, tested by EIA method, was 17.1%(26/152) The incidence of chlamydial infection was 25.0% in cervicities c purulent discharge, 24.4% in vaginitis c trichomonas and 13.3% in non-specific vaginitis, but none in clean cases of pregnancy and infertility. The incidence of chlamydial infection in each age group was 14.3% in 20th, 15.4% in 30th, 20.3% in 40th and 18.8% in the age over 50.

Clinical characteristics and follow-up of Korean patients with adrenal incidentalomas

( Yoon Young Cho ),( Sung Hwan Suh ),( Ji Young Joung ),( Hyem In Jeong ),( Dong Mo Je ),( Hong Seok Yoo ),( Taek Kyu Park ),( Yong Ki Min ),( Kwang Won Kim ),( Jae Hyeon Kim ) 대한내과학회 2013 The Korean Journal of Internal Medicine Vol.28 No.5

Background/Aims: We investigated the clinical characteristics and follow-up findings of subjects with adrenal incidentalomas in a single, tertiary-care hospital in South Korea. Methods: The study consisted of a retrospective analysis of 282 adrenal incidentaloma patients who underwent radiographic and endocrinological evaluations at Samsung Medical Center in Seoul, South Korea, between January 2004 and July 2011. Results: Most (86.2%) of the subjects were found to have nonfunctioning tumors. Functioning tumors were seen in 39 patients (13.8%). Among them, 28 (9.9%) had subclinical Cushing syndrome (SCS), six (2.1%) had pheochromocytoma, and five (1.8%) had primary hyperaldosteronism. Malignant adrenal tumors were discovered in three cases: two (0.7%) were primary adrenal cancers, and one (0.4%) was a secondary metastasis from a lung cancer. Significant risk factors for functional tumors were female gender (odds ratio [OR], 3.386; 95% confidence interval [CI], 1.611 to 7.117; p = 0.0013) and a noncontrast attenuation value of > 10 Hounsfield units (OR, 2.806; 95% CI, 1.231 to 6.397; p = 0.0141). During follow-up (mean, 22.5 months) of 72 of the patients, three (4.2%) developed hormonal changes due to functional tumors. One was confirmed as pheochromocytoma by histopathology, and the others were diagnosed with SCS and followed routinely without surgical intervention. No malignant transformation was found in these patients. Conclusions: Based on these findings, initial hormonal and radiographic evaluations for adrenal incidentalomas appear to be more important than follow-up tests because functional or malignant changes are rare.

실험 : 신생 흰쥐의 산소유발성 망막증에서 항세포사멸사를 통한 Minocycline의 망막 보호효과

장윤영 ( Yoon Young Jang ),서억수 ( Eok Soo Suh ),김우택 ( Woo Taek Kim ) 대한주산의학회 2010 大韓周産醫學會雜誌 Vol.21 No.1

목적: 미숙아망막병증은 고농도 산소 투여를 받은 미숙아에서 망막에 비정상적인 신생혈관이 생기는 경우로 근래는 적절한 산소 농도 조절로 많이 감소했다가 최근 다시 신생아학 발달로 미숙아 생존이 높아짐으로 증가하고 있다. 신생혈관은 육아 조직을 동반하여 유리체 내로 들어가 섬유조직으로 변하면서 망막을 견인하여 망막을 박리하며, 심한 경우 시력이 완전 소실된다. 주요 선행인자로 미성숙과 산소치료, 산혈증, 고이산화탄소혈증, 수혈, 비타민 E 결핍, 빛 등이 알려져 있으며, 혈관 미성숙의 정도, 산소투여기간 및 산소농도 등이 매우 중요하게 작용하고 있다. 이에 연구자는 신생 흰쥐을 이용하여 생체 내·외 산소유발성 망막병증을 유발하고, 미숙아망막증의 항세포사멸사 기전을 통한 minocycline 의 망막 보호 효과를 알아보고자 하였다. 방법: 생체내 산소유발성 망막증은 생후 1일째의 신생 흰쥐를 생후 2주까지 24시간 주기로 고산소와 정상산소(산소분압 80%와 21%) 환경에 교대로 노출시킨 후(H군, HM군) 정상산소 환경으로 옮겨 사육하였고, 대조군(N군)은 정상산소 환경에서 사육하였다. Minocycline (HM군) 약물을 1주일간씩 투여하였고 생후 3주째 동물을 희생시켜 망막내 신경세포들의 형태학적 변화는 H&E 염색법을 이용하여 전자현미경으로 살펴보았다. 생체외 산소유발성 망막증은 재태기간 0-2일된 신생 흰쥐의 망막세포를 추출하여 정상산소군(N군)은 5% 이산화탄소 배양기에 두고, 고산소군(H군)과 minocycline(HM군) 약물을 투여한 군은 5% 이산화탄소 배양기내 100% 산소 용기에 두어 산소에 의한 망막세포 손상을 유발하였다. 동물모델 및 세포배양 모두 세포사멸사와 관련된 Bcl-2, Bax, caspase-3 항체 및 primer를 이용하여 Western blotting과 실시간 중합효소연쇄반응을 시행하였다. 결과: 산소유발성 망막증의 생체내·외 실험에서 Western blotting 및 실시간 중합효소연쇄반응을 실시한 결과로 Bcl-2, Bax, caspase-3의 발현은 고산소군보다 minocycline을 투여한 군에서 Bcl-2는 증가, Bax 및 caspase-3는 감소하였다. 결론: 본 연구에서 minocycline은 항세포사멸사 기전을 통하여 미숙아망막병증에서 나타나는 비정상적인 혈관 신생을 보호하는 것을 알 수 있었다. Purpose: Retinopathy of prematurity (ROP) is a leading cause of blindness with retinal detachment due to oxygen toxicity in preterm infants. Recently advances in neonatal care had to led improved survival rates in premature infants and ROP re-emerged as a significant clinical problem. In the present study, we aimed to determine the protective abilities of minocycline in a animal model of ROP and a primary retinal cell cultures of neonatal rat via anti-apoptotic actions using Western blotting and real-time PCR with Bcl-2, Bax and caspase-3 antibodies and mRNAs. Methods: In the in vivo oxygen-induced retinopathy (OIR), the cyclic hyperoxia was performed that 80% O2 for 1 day and 21% O2 for 1 day from P1-14 of newborn rats. Minocycline was injected intravitreously for 7 days and sacrificed at P21. In the in vitro OIR, primary retinal cell culture was done using P0-P2 SD rats. Hyperoxia injury was done for 100% O2 exposure for 6 hours. Western blotting and real-time PCR using Bcl-2, Bax and caspase-3 antibody and primer were done in the rat model of ROP and the dispersed retinal cell culture. To identify photoreceptors of retinal cells the immunofluorescence assay photoreceptor marker, IRBP, was used. Results: In the in vivo OIR, the expression of Bcl-2 antibody and mRNA was increased and those of Bax and caspase-3 were reduced in the minocycline-treated group. In the in vitro OIR, the result was the same as above. Conclusion: In conclusion, minocycline was suggested to have retinal protective effects for hyperoxic injury via anti-apoptotic mechanism.