Dynamic analysis of Ca^(2+) level during bovine oocytes maturation and early embryonic development

Su Li Liang,Qian Jun Zhao,Xiang Chen Li,Ya Ping Jin,Yi Peng Wang,Xiao Hua Su,Wei Jun Guan,Yue Hui Ma 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2

Mammalian oocyte maturation and early embryo development processes are Ca^(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca^(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca^(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca^(2+)was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca^(2+) was present throughout the blastomere. In PA embryos, Ca^(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca^(2+) in the SCNT embryos. However, Ca^(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca^(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca^(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.

Robust Model Predictive Control for Multi-phase Batch Processes with Asynchronous Switching

Hui Li,Lijun Song,Xueying Jiang,Huiyuan Shi,Chengli Su,Ping Li 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.1

A robust asynchronous switching model predictive controller is designed for multi-phase batch processes with uncertainties, unknown disturbances and time-varying set-point. Firstly, in view of the influence of timevarying set-point and disturbances, an asynchronous switching model with stable and unstable cases is established. Based on the switching model, a robust asynchronous switching model predictive control law is designed. Secondly, by using relevant theories and methods, the sufficient conditions with the form of linear matrix inequality (LMI) are given to ensure that the multi-phase batch processes are asymptotically stable at each phase and exponentially stable at each batch. Then, these LMI conditions are solved online to obtain the control gain of each phase, the shortest running time of each stable case, and the longest running time of each unstable case. Finally, the effectiveness and feasibility of the proposed method are verified by taking the injection molding process as an example.

Identification of QTLs associated with heat tolerance at the heading and flowering stage in rice (Oryza sativa L.)

Li, Mao-mao,Li, Xia,Yu, Li-qin,Wu, Jin-wen,Li, Hui,Liu, Jin,Ma, Xiao-ding,Jo, Su-min,Park, Dong-Soo,Song, Youchun,Shin, Dongjin,Han, Long-zhi Springer-Verlag 2018 Euphytica Vol.214 No.4

Decitabine in the Treatment of Acute Myeloid Leukemia and Myelodysplastic Syndromes, Which Combined with Complex Karyotype Respectively

Gao, Su,Li, Zheng,Fu, Jian-Hong,Hu, Xiao-Hui,Xu, Yang,Jin, Zheng-Ming,Tang, Xiao-Wen,Han, Yue,Chen, Su-Ning,Sun, Ai-Ning,Wu, De-Pei,Qiu, Hui-Ying Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: We conducted a study exploring the clinical safety and efficacy of decitabine in patients with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), combined with a complex karyotype. Materials and Methods: From April 2009 to September 2013, a total of 35 patients with AML/MDS combined with a complex karyotype diagnosed in the First Affiliated Hospital of Soochow University were included for retrospective analysis. All patients were treated with decitabine alone ($20mg/m^2$ daily for 5 days) or combination AAG chemotherapy (Acla 20mg qod*4d, Ara-C $10mg/m^2$ q12h*7d, G-CSF $300{\mu}g$ qd, the dose of G-CSF adjusted to the amount in blood routinely). Results: In 35 patients, 15 exhibited a complete response (CR), and 6 a partial response (PR), the overall response rate (CR+PR) being 60% (21 of 35). Median disease-free survival was 18 months and overall survival was 14 months. In the 15 MDS patients with a complex karyotype, the CR rate was 53.3% (8 of 15); in 20 AML patients with complex karyotype, the overall response rate was 65% (13 of 20). The response rate of decitabine alone (22 cases) was 56.5% (13 of 22), while in the combination chemotherapy group (13 cases), the effective rate was 61.5% (8 of 13)(P>0.05). There are 15 patients with chromosome 7 aberration, after treatment with decitabine, 7 CR, 3 PR, overall response rate was 66.7% (10 of 15). Of 18 patients with 3 to 5 kinds of chromosomal abnormalities, 66.7% demonstrated a response; of 17 with more than 5 chromosomal abnormalities, 52.9% had a response. In the total of 35 patients, with one course (23 patients) and ${\geq}$two courses (12 patients), the overall response rate was 40.9% and 92.3% (P<0.05). Grade III to IV hematological toxicity was observed in 27 cases (75%). Grade III to IV infections were clinically documented in 7 (20%). Grades I to II non-hematological toxicity were infections (18 patients), haematuria (2 patients), and bleeding (3 patients). With follow-up until September 2013, 7 patients were surviving, 18 had died and 10 were lost to follow-up. In the 6 cases who underwent allogeneic hematopoietic stem cell transplantation (HSCT) all were still relapse-free survivors. Conclusions: Decitabine alone or combination with AAG can improve outcome of AML/MDS with a complex karyotype, there being no significant difference decitabine in inducing remission rates in patients with different karyotype. Increasing the number of courses can improve efficiency. This approach with fewer treatment side effects in patients with a better tolerance should be employed in order to create an improved subsequent chance for HSCT.

The Stent-Assisted Coil-Jailing Technique Facilitates Efficient Embolization of Tiny Cerebral Aneurysms

Cong-Hui Li,Xian-Hui Su,Bo Zhang,Yong-Feng Han,Er-Wei Zhang,Lei Yang,Dong-Liang Zhang,Song-Tao Yang,Zhen-Quan Yan,Bu-Lang Gao 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

Objective: Tiny cerebral aneurysms are difficult to embolize because the aneurysm’s sac is too small for a single small coil, and coils within the aneurysm may escape from the confinement of a stent. This study was performed to introduce the stent-assisted coil-jailing technique and to investigate its effect on the coil embolization of tiny intracranial aneurysms. Materials and Methods: Sixteen patients with tiny intracranial aneurysms treated with the stent-assisted coil-jailing technique between January 2011 and December 2013 were retrospectively reviewed and followed-up. Results: All aneurysms were successfully treated with the coil-jailing technique, and at the end of embolization, complete occlusion of the aneurysm was achieved in 9 cases (56.3%), incomplete occlusion in 6 (37.5%), and partial occlusion in 1 (6.3%). Intraprocedural complications included acute thrombosis in one case (6.3%) and re-rupture in another (6.3%). Both complications were managed appropriately with no sequela. Follow-up was performed in all patients for 3–24 months (mean, 7.7 months) after embolization. Complete occlusion was sustained in the 9 aneurysms with initial complete occlusion, progressive thrombosis to complete occlusion occurred in the 6 aneurysms with initial near-complete occlusion, and one aneurysm resulted in progressive thrombosis to complete occlusion after initial partial occlusion. No migration of stents or coils occurred at follow-up as compared with their positions immediately after embolization. At follow-up, all patients had recovered with no sequela. Conclusion: The stent-assisted coil-jailing technique can be an efficient approach for tiny intracranial aneurysms, even though no definite conclusion regarding its safety can be drawn from the current data.

SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin–Rho GTPase–Hippo Pathways

Zhang, Yan-Li,Li, Qing,Yang, Xiao-Mei,Fang, Fang,Li, Jun,Wang, Ya-Hui,Yang, Qin,Zhu, Lei,Nie, Hui-Zhen,Zhang, Xue-Li,Feng, Ming-Xuan,Jiang, Shu-Heng,Tian, Guang-Ang,Hu, Li-Peng,Lee, Ho-Young,Lee, Su-J American Association for Cancer Research 2018 Cancer research Vol.78 No.9

<P>Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P>Tumor-associated macrophages (TAM) represent key regulators of the complex interplay between cancer and the immune microenvironment. Matricellular protein SPON2 is essential for recruiting lymphocytes and initiating immune responses. Recent studies have shown that SPON2 has complicated roles in cell migration and tumor progression. Here we report that, in the tumor microenvironment of hepatocellular carcinoma (HCC), SPON2 not only promotes infiltration of M1-like macrophages but also inhibits tumor metastasis. SPON2-α4β1 integrin signaling activated RhoA and Rac1, increased F-actin reorganization, and promoted M1-like macrophage recruitment. F-Actin accumulation also activated the Hippo pathway by suppressing LATS1 phosphorylation, promoting YAP nuclear translocation, and initiating downstream gene expression. However, SPON2-α5β1 integrin signaling inactivated RhoA and prevented F-actin assembly, thereby inhibiting HCC cell migration; the Hippo pathway was not noticeably involved in SPON2-mediated HCC cell migration. In HCC patients, SPON2 levels correlated positively with prognosis. Overall, our findings provide evidence that SPON2 is a critical factor in mediating the immune response against tumor cell growth and migration in HCC.</P><P><B>Significance:</B> Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P><B>Graphical Abstract:</B> http://cancerres.aacrjournals.org/content/canres/78/9/2305/F1.large.jpg. <I>Cancer Res; 78(9); 2305–17. ©2018 AACR</I>.</P><P><B>Graphical Abstract</B></P><P> [Figure]</P>

Ramp-Strike Risk Evaluation Making of Carrier-Based Aircraft

Hai-tao Jiang,Xiao-dong Su,Hui Li 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

To make “Ramp-Strike Risk” evaluation making accurately, this paper introduces a new wave-off risk assessment method based on neural network of carrier-based aircraft. On account of defining “Wave-Off Retain Clearance”, the wave-off operation system with military power and vertical elevator is established as evaluation medium. The “Wave-Off Risk State Area” established by limited wave-off envelope can reduce network training burden, and the quantitative “Ramp-Strike Risk” function with flight state is presented through BP neural network finally. Simulation results show that the risk with flight states can be predicted by using new estimation method, moreover auxiliary rectification and early-warning for landing is provided.

Nematicidal activity against Aphelenchoides besseyi and Ditylenchus destructor of three biflavonoids, isolated from roots of Stellera chamaejasme

Hui Jin,Haiyan Cui,Xiaoyan Yang,Lihong Xu,Xudong Li,Rentao Liu,Zhiqiang Yan,Xiuzhuang Li,Weili Zheng,Yuhui Zhao,Xiaoxia Song,Lihua Zhong,Anxiang Su,Bo Qin 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

Aphelenchoides besseyi and Ditylenchus destructor can cause serious problems for a number of important agricultural crops and vegetables. In this study, the ethanol extract of Stellera chamaejasme L. roots showed strong nematicidal activity against Aphelenchoides besseyi and Ditylenchus destructor. By using a bioactivity-driven fractionation, three biflavonoids were isolated from the extract and their structures were identified by mass and nuclear magnetic resonance spectral data. Nematicidal activity bioassays revealed that isoneochamaejasmin A had the strongest nematicidal activity against A. besseyi and D. destructor with LC 50 values of 2.32 and 0.18 mM at 72 h, respectively. Chamaejasmenin B displayed weaker nematicidal activity against A. besseyi with an LC 50 value of 3.94 mM at 72 h. Neochamaejasmin B induced the lowest mortality against D. destructor with an LC 50 values of 15.6 mM at 72 h. These results suggested that the kind and position of substitutions and the relative configuration of 2-H/3-H and 2”-H/3”-H could be considered as important factors responsible for the nematicidal activity of these purified C-3/C-3″ biflavonoids.

The Study on Stress in Clinical Practice Internship and Professional Commitment among Graduating Nursing Students of a 5-year Junior College in Southern Taiwan

Su Hui Cheng,Li-Ling Liao,I-Ju Lai 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

Asymmetric Variable Universe Adaptive Landing Fuzzy Controller for Carrier-Based Aircraft

Hui Li,Xiao-dong Su,Hai-tao Jiang 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.5

During the process of aircraft landing, the control manners of carrier-based aircraft are always used to study. This is one of the important ways to avoid pilot-aircraft adverse coupling. In order to keep the accuracy of landing, this paper presents an improved asymmetric variable universe adaptive landing fuzzy controller with Safe Flight Area during approach for carrier-based aircraft. By means of universe-conversion factors and contraction–expansion factors, universe of discourse can be modified online, and fuzzy rules can reproduce automatically to adapt to the modified universe of discourse. The model simulation results indicate the better performance of the new method in comparison with the traditional controller with more accuracy and practicability.