Ginsenoside Rg3 nanoparticles with permeation enhancing based chitosan derivatives were encapsulated with doxorubicin by thermosensitive hydrogel and anti-cancer evaluation of peritumoral hydrogel injection combined with PD-L1 antibody

Wu Hao,Wei Guoli,Luo Lixia,Li Lingchang,Gao Yibo,Tan Xiaobin,Wang Sen,Chang Haoxiao,Liu Yuxi,Wei Yingjie,Song Jie,Zhang Zhenhai,Huo Jiege 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Combination of chemotherapy and immune checkpoint inhibitor therapy has greatly improved the anticancer effect on multiple malignancies. However, the efficiency on triple-negative breast cancer (TNBC) is limited, since most patients bear “cold” tumors with low tumor immunogenicity. Doxorubicin (DOX), one of the most effective chemotherapy agents, can induce immunogenic cell death (ICD) and thus initiating immune response.In this study, to maximize the ICD effect induced by DOX, chitosan and cell-penetrating peptide (R6F3)-modified nanoparticles (PNPs) loaded with ginsenoside Rg3 (Rg3) were fabricated using the self-assembly technique, followed by co-encapsulation with DOX based on thermo-sensitive hydrogel. Orthotopic tumor model and contralateral tumor model were established to observe the antitumor efficacy of the thermo-sensitive hydrogel combined with anti-PD-L1 immunotherapy, besides, the biocompatibility was also evaluated by histopathological.Rg3-PNPs strengthened the immunogenic cell death (ICD) effect induced by DOX. Moreover, the hydrogel co-loading Rg3-PNPs and DOX provoked stronger immune response in originally nonimmunogenic 4T1 tumors than DOX monotherapy. Following combination with PD-L1 blocking, substantial antitumor effect was achieved due to the recruitment of memory T cells and the decline of adaptive PD-L1 enrichment.The hydrogel encapsulating DOX and highly permeable Rg3-PNPs provided an efficient strategy for remodeling immunosuppressive tumor microenvironment and converting immune “cold” 4T1 into “hot” tumors.

Embossed Structural Skin for Tall Buildings

Song, Jin Young,Lee, Donghun,Erikson, James,Hao, Jianming,Wu, Teng,Kim, Bonghwan Council on Tall Building and Urban Habitat Korea 2018 International journal of high-rise buildings Vol.7 No.1

This paper explores the function of a structural skin with an embossed surface applicable to use for tall building structures. The major diagrid system with a secondary embossed surface structure provides an enhanced perimeter structural system by increasing tube section areas and reduces aerodynamic loads by disorienting major organized structure of winds. A parametric study used to investigate an optimized configuration of the embossed structure revealed that the embossed structure has a structural advantage in stiffening the structure, reducing lateral drift to 90% compared to a non-embossed diagrid baseline model, and results of wind load analysis using computational fluid dynamics, demonstrated the proposed embossed system can reduce. The resulting undulating embossed skin geometry presents both opportunities for incorporating versatile interior environments as well as unique challenges for daylighting and thermal control of the envelope. Solar and thermal control requires multiple daylighting solutions to address each local façade surface condition in order to reduce energy loads and meet occupant comfort standards. These findings illustrate that although more complex in geometry, architects and engineers can produce tall buildings that have less impact on our environment by utilizing structural forms that reduce structural steel needed for stiffening, thus reducing embodied $CO^2$, while positively affecting indoor quality and energy performance, all possible while creating a unique urban iconography derived from the performance of building skin.

Concurrent Hypermethylation of SFRP2 and DKK2 Activates the Wnt/β-Catenin Pathway and Is Associated with Poor Prognosis in Patients with Gastric Cancer

Hao Wang,Xiang-Long Duan,Xiao-Li Qi,Lei Meng,Yi-Song Xu,Tong Wu,Peng-Gao Dai 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.1

Aberrant hypermethylation of Wnt antagonists has been observed in gastric cancer. A number of studies have focused on the hypermethylation of a single Wnt antagonist and its role in regulating the activation of signaling. However, how the Wnt antagonists interacted to regulate the signaling pathway has not been reported. In the present study, we systematically investigated the methylation of some Wnt antagonist genes (SFRP2, SFRP4, SFRP5, DKK1, DKK2, and APC) and their regulatory role in carcinogenesis. We found that aberrant promoter methylation of SFRP2, SFRP4, DKK1, and DKK2 was significantly increased in gastric cancer. Moreover, concurrent hypermethylation of SFRP2 and DKK2 was observed in gastric cancer and this was significantly associated with increased expression of -catenin, indicating that the joint inactivation of these two genes promoted the activation of the Wnt signaling pathway. Further analysis using a multivariate Cox proportional hazards model showed that DKK2 methylation was an independent prognostic factor for poor overall survival, and the predictive value was markedly enhanced when the combined methylation status of SFRP2 and DKK2 was con-sidered. In addition, the methylation level of SFRP4 and DKK2 was correlated with the patient’s age and tumor differentiation, respectively. In conclusion, epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival.

WHEN AI INFLUENCERS AS AD ENDORSERS: THE INTERACTIVE EFFECTS OF PRODUCT CATEGORIES AND AD APPEALS ON AD ATTITUDE

Yushi Song,Hao Wu,Guoxin Li 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07

This study constructs a model to predict ad attitude when AI influencers act as ad endorsers. In the results, search products and rational ad appeal have more positive ad attitude, perceived empathy and perceived expertise as mediator. These three variables can be reinforced by the consistency of ad appeals and product categories.

The characteristic analysis of the operational overvoltage caused by the vacuum circuit breakers on High-speed trains

Xiao Song,Tong Mengyuan,Rao Yang,Li Yuhang,Ye Zhizong,Shen Yixiang,Hou Hao,Gao Guoqiang,Wu Guangning,Jin Yaoyao,Zhou Jie,Liu Jie 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.1

Along with the operating velocity and traction power increasing continuously, the issue related to the safety and reliability of the high-voltage traction power system for high-speed trains have gradually been exposed during the practical service process. When the train passes a neutral section settled roughly per 15 km between two neighboring substations, the on-board vacuum circuit breaker (VCB) must to be operated for changing the input powers with diff erent phases. At the moment of switching on or off the VCB, the arc between the contacts in the arc-extinguishing chamber may be triggered, which tends to result in the operational overvoltage. Due to numerous inductances and capacitances existing in the traction power supply system, the overvoltage may lead to high-frequency electromagnetic oscillation spreading along the transmission routes. The on-board high-voltage equipments within the power supply system may suff er from the impulse brought from the VCB operational overvoltage frequently, which is possible to cause the insulation aging or breakdown issue. To investigate the characteristic of the operational overvoltage of VCBs, a ‘train-rail-catenary’ power supply model is built based on the measured impedance parameters, which has been verifi ed by the experimental tests. The generating mechanism with the infl uence factors of the operational overvoltage when switching on or off the VCB has been explored, meanwhile the distribution of the train body overvoltage on each carriage has also been presented.

Effect of oxidizing treatment on electrocatalytic activity of boron‑doped amorphous carbon thin films

Chen‑Song Wang,Ni Suo,Hao Huang,Ai‑min Wu,Guo‑Zhong Cao,Gui‑Feng Zhang 한국탄소학회 2019 Carbon Letters Vol.29 No.5

Boron-doped amorphous carbon (BDAC) thin films with a regular oxygen reduction reaction (ORR) catalytic activity were synthesized in a hot filament chemical vapor deposition device using a mixture of CH4 and H2 as a gas source and B2O3 as a boron source and then oxidized in air at 380–470 °C for 15–75 min. Scanning electron microscope, transmission electron microscope, Raman spectroscopy, X-ray photoelectron spectroscopy, and electrochemical tests were used to characterize the physical and electrochemical properties of the BDAC catalysts. It was concluded that the BDAC catalyst oxidized at 450 °C for 45 min showed the best ORR catalytic activity in alkaline medium. The oxygen reduction potential and the transfer electron number n, respectively, are − 0.286 V versus Ag/AgCl and 3.24 from the rotating disk electrode experiments. The treated carbon film has better methanol resistance and stability than the commercial Pt/C catalyst.

Concurrent Hypermethylation of SFRP2 and DKK2 Activates the Wnt/β-Catenin Pathway and Is Associated with Poor Prognosis in Patients with Gastric Cancer

Wang, Hao,Duan, Xiang-Long,Qi, Xiao-Li,Meng, Lei,Xu, Yi-Song,Wu, Tong,Dai, Peng-Gao Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.1

Aberrant hypermethylation of Wnt antagonists has been observed in gastric cancer. A number of studies have focused on the hypermethylation of a single Wnt antagonist and its role in regulating the activation of signaling. However, how the Wnt antagonists interacted to regulate the signaling pathway has not been reported. In the present study, we systematically investigated the methylation of some Wnt antagonist genes (SFRP2, SFRP4, SFRP5, DKK1, DKK2, and APC) and their regulatory role in carcinogenesis. We found that aberrant promoter methylation of SFRP2, SFRP4, DKK1, and DKK2 was significantly increased in gastric cancer. Moreover, concurrent hypermethylation of SFRP2 and DKK2 was observed in gastric cancer and this was significantly associated with increased expression of ${\beta}-catenin$, indicating that the joint inactivation of these two genes promoted the activation of the Wnt signaling pathway. Further analysis using a multivariate Cox proportional hazards model showed that DKK2 methylation was an independent prognostic factor for poor overall survival, and the predictive value was markedly enhanced when the combined methylation status of SFRP2 and DKK2 was considered. In addition, the methylation level of SFRP4 and DKK2 was correlated with the patient's age and tumor differentiation, respectively. In conclusion, epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival.

Effect of oxidizing treatment on electrocatalytic activity of boron-doped amorphous carbon thin films

Wang Chen-Song,Suo Ni,Huang Hao,Wu Ai-min,Cao Guo-Zhong,Zhang Gui-Feng 한국탄소학회 2019 Carbon Letters Vol.29 No.5

Boron-doped amorphous carbon (BDAC) thin films with a regular oxygen reduction reaction (ORR) catalytic activity were synthesized in a hot filament chemical vapor deposition device using a mixture of CH4 and H2 as a gas source and B2O3 as a boron source and then oxidized in air at 380–470 °C for 15–75 min. Scanning electron microscope, transmission electron microscope, Raman spectroscopy, X-ray photoelectron spectroscopy, and electrochemical tests were used to characterize the physical and electrochemical properties of the BDAC catalysts. It was concluded that the BDAC catalyst oxidized at 450 °C for 45 min showed the best ORR catalytic activity in alkaline medium. The oxygen reduction potential and the transfer electron number n, respectively, are − 0.286 V versus Ag/AgCl and 3.24 from the rotating disk electrode experiments. The treated carbon film has better methanol resistance and stability than the commercial Pt/C catalyst.

Plasma metabolites associated with physiological and biochemical indexes indicate the effect of caging stress on mallard ducks (Anas platyrhynchos)

Zheng Chao,Wu Yan,Liang Zhen Hua,Pi Jin Song,Cheng Shi Bin,Wei Wen Zhuo,Liu Jing Bo,Lu Li Zhi,Zhang Hao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks. Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database. Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted. Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks.Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database.Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism.Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted.

Inhalation of panaxadiol alleviates lung infl ammation via inhibiting TNFA/ TNFAR and IL7/IL7R signaling between macrophages and epithelial cells

Yifan Wang,Hao Wei,Zhen Song,Liqun Jiang,Mi Zhang,Xiao Lu,Wei Li,Yuqing Zhao,Lei Wu,Shuxian Li,Huijuan Shen,Qiang Shu,Yicheng Xie 고려인삼학회 2024 Journal of Ginseng Research Vol.48 No.1

Background: Lung inflammation occurs in many lung diseases, but has limited effective therapeutics. Ginseng andits derivatives have anti-inflammatory effects, but their unstable physicochemical and metabolic propertieshinder their application in the treatment. Panaxadiol (PD) is a stable saponin among ginsenosides. Inhalationadministration may solve these issues, and the specific mechanism of action needs to be studied. Methods: A mouse model of lung inflammation induced by lipopolysaccharide (LPS), an in vitro macrophageinflammation model, and a coculture model of epithelial cells and macrophages were used to study the effectsand mechanisms of inhalation delivery of PD. Pathology and molecular assessments were used to evaluate efficacy. Transcriptome sequencing was used to screen the mechanism and target. Finally, the efficacy andmechanism were verified in a human BALF cell model. Results: Inhaled PD reduced LPS-induced lung inflammation in mice in a dose-dependent manner, includinginflammatory cell infiltration, lung tissue pathology, and inflammatory factor expression. Meanwhile, the dose ofinhalation was much lower than that of intragastric administration under the same therapeutic effect, which maybe related to its higher bioavailability and superior pharmacokinetic parameters. Using transcriptome analysisand verification by a coculture model of macrophage and epithelial cells, we found that PD may act by inhibitingTNFA/TNFAR and IL7/IL7R signaling to reduce macrophage inflammatory factor-induced epithelial apoptosisand promote proliferation. Conclusion: PD inhalation alleviates lung inflammation and pathology by inhibiting TNFA/TNFAR and IL7/IL7Rsignaling between macrophages and epithelial cells. PD may be a novel drug for the clinical treatment of lunginflammation.