Gold Nanoclusters Promote Electrocatalytic Water Oxidation at the Nanocluster/CoSe₂ Interface

Zhao, Shuo,Jin, Renxi,Abroshan, Hadi,Zeng, Chenjie,Zhang, Hui,House, Stephen D.,Gottlieb, Eric,Kim, Hyung J.,Yang, Judith C.,Jin, Rongchao American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.3

<P>Electrocatalytic water splitting to produce hydrogen comprises the hydrogen and oxygen evolution half reactions (HER and OER), with the latter as the bottleneck process. Thus, enhancing the OER performance and understanding the mechanism are critically important. Herein, we report a strategy for OER enhancement by utilizing gold nanoclusters to form cluster/CoSe2 composites; the latter exhibit largely enhanced OER activity in alkaline solutions. The Au-25/CoSe2 composite affords a current density of 10 mA cm(-2) at small overpotential of, similar to 0.43 V (cf. CoSe2: similar to 0.52 V). The ligand and gold cluster size can also tune the catalytic performance of the composites. Based upon XPS analysis and DFT simulations, we attribute the activity enhancement to electronic interactions between nanocluster and CoSe2, which favors the formation of the important intermediate (OOH) as well as the desorption of oxygen molecules over Au-11/CoSe2 composites in the process of water oxidation. Such an atomic level understanding may provide some guidelines for design of OER catalysts.</P>

Using SiNx double-layer deposition to reduce electrode incidence of short circuits due to impurity particles in thin film transistor preparation

Zhao Shuo,Yu Xiang,Pei Jingxuan,Yang Lei,Zhang Zhiqiang,Zhang Pingping 한국물리학회 2023 Current Applied Physics Vol.48 No.-

Aviation safety relies on accurate scope presentation of air navigation displays, and lack of display information due to poor dot line may cause the pilot to misjudge the air conditions. Impurity particles occurred during preparing thin film transistor (TFT) circuit may be a leading reason for poor dot line. This work attempts to replace existing SiNx single-layer deposition with double-layer, so that the incidence risk can be lower through reducing number and size of the impurity particles. Double-layer deposition approach has the dot line structures and performances to that of single-layer, and can meet the requirements of display usage. Results of field test demonstrate that double-layer deposition reduces defect rate of display more efficient than single-layer. Proposed mechanism of double-layer deposition illuminates that 2nd-SiNx can cover holes generated by falling impurity particles to avoid short circuits in both indium tin oxide (ITO) and source-drain (SD) contact.

A detection method of Escherichia coli O157:H7 based on immunomagnetic separation and aptamers-gold nanoparticle probe quenching Rhodamine B’s fluorescence

Fengnan Lian,Dan Wang,Shuo Yao,Lirui Ge,Yue Wang,Yuyi Zhao,Jinbin Zhao,Xiuling Song,Chao Zhao,Jinhua Li,Yajuan Liu,Minghua Jin,Kun Xu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8

This research aimed to detect Escherichia coli O157:H7 in milk based on immunomagnetic probe separation technology and quenching effect of gold nanoparticles to Rhodamine B. Streptavidin-modified magnetic beads (MBs) were combined with biotin-modified antibodies to capture E. coli O157:H7 specifically. Gold nanoparticle (AuNPs) was incubated with sulfhydryl-modified aptamers (SH-Aptamers) to obtain the Aptamers-AuNPs probe. After magnetic beads captured target bacteria and formed a sandwich structure with the gold nanoprobe, Rhodamine B was added into complex to obtain fluorescent signal changes. Our results demonstrated that the established method could detect E. coli O157:H7 in the range of 101–107 CFU/mL, and the limit of detection (LOD) was 0.35 CFU/mL in TBST buffer (pH = 7.4). In milk simulation samples, the LOD of this method was 1.03 CFU/mL. Our research provides a promising approach on the detection of E. coli O157:H7.

Functional Investigation on Aromatase in Endometrial Hyperplasia in Polycystic Ovary Syndrome Cases

Zhao, Pan-Lin,Zhang, Qiu-Fang,Yan, Li-Ying,Huang, Shuo,Chen, Yuan,Qiao, Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Objective: To explore the possible significance of aromatase P450 in endometrial hyperplasia with a background of polycystic ovary syndrome (PCOS). Methods: Immunohistochemistry was used to determine the expression of aromatase P450 in endometrium of PCOS patients. Semiquantitative analysis of aromatase P450 expression of mRNA and protein level wasalso carried out by real-time quantitative RT-PCR method. After endometrial cells were stimulated by testosterone and letrozole in vitro, the estradiol ($E_2$) level was determined, and the expression of cell aromatase P450 mRNA was assessed. Results: The aromatase P450 mRNA level was increased in endometria of PCOS patients. When endometrial cells were cultured with $10^{-6}M$ testosterone, the $E_2$ level in the culture medium increased. An inhibitory effect on $E_2$ generation and expression of aromatase P450 mRNA was observed when the endometrial cells were treated with $10^{-5}M$ letrozole. Conclusions: There is an increased expression of aromatase P450 in PCOS patient endometrium. Androgen stimulation could enhance the synthesis of aromatase P450 mRNA and the production of $E_2$ in endometrial cells in vitro while letrozole could do the reverse.

Fabrication of Protective Textile with N-doped TiO2 Embedded Citral Microcapsule Coating and Its Air Purification Properties

Shuo Wang,Huihui Ding,Yishi Zhao,Yonggui Li,Wei Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2

The protective textile was successfully prepared using N-doped TiO2 (N-TiO2) embedded citral microcapsule coating. N-TiO2 loaded citral microcapsules were synthesized through interfacial polymerization. Results showed the average diameter of N-TiO2 loaded citral microcapsule with core/shell (Mcitral : MHDI) 2:1 was 1.069 μm. Its encapsulation efficiency was 75.63 %. The citral content from finished cotton fabric measured by gas chromatography was up to 2.90 mg/g (weight of essence: weight of fabric). The essence release content of citral was nearly 50 % of their aroma after 12 weeks, exhibiting good physical function for air purification. Additionally, aromatic textile coated with N-TiO2 embedded citral microcapsules also exhibited photocatalytic activity for the degradation of formaldehyde under visible light irradiation. This functionalfabric would have potential applications in the air purification filed due to the chemical and physical dual effect.

Thioredoxin A of Streptococcus suis Serotype 2 Contributes to Virulence by Inhibiting the Expression of Pentraxin 3 to Promote Survival Within Macrophages

Zhao Chijun,Jia Xinglin,Pan Yanying,Liao Simeng,Zhang Shuo,Ji Chunxiao,Kuang Guangwei,Wu Xin,Liu Quan,Tang Yulong,Fang Lihua 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that can infect humans in contact with infected pigs or their byproducts. It can employ different types of genes to defend against oxidative stress and ensure its survival. The thioredoxin (Trx) system is a key antioxidant system that contributes adversity adaptation and pathogenicity. SS2 has been shown to encode putative thioredoxin genes, but the biological roles, coding sequence, and underlying mechanisms remains uncharacterized. Here, we demonstrated that SSU05_0237-ORF, from a clinical SS2 strain, ZJ081101, encodes a protein of 104 amino acids with a canonical CGPC active motif and an identity 70–85% similar to the thioredoxin A (TrxA) in other microorganisms. Recombinant TrxA efficiently catalyzed the thiol-disulfide oxidoreduction of insulin. The deletion of TrxA led to a significantly slow growth and markedly compromised tolerance of the pathogen to temperature stress, as well as impaired adhesion ability to pig intestinal epithelial cells (IPEC-J2). However, it was not involved in H2O2 and paraquat-induced oxidative stress. Compared with the wild-type strain, the ΔTrxA strain was more susceptible to killing by macrophages through increasing NO production. Treatment with TrxA mutant strain also significantly attenuated cytotoxic effects on RAW 264.7 cells by inhibiting inflammatory response and apoptosis. Knockdown of pentraxin 3 in RAW 264.7 cells was more vulnerable to phagocytic activity, and TrxA promoted SS2 survival in phagocytic cells depending on pentraxin 3 activity compared with the wild-type strain. Moreover, a co-inoculation experiment in mice revealed that TrxA mutant strain is far more easily cleared from the body than the wild type strain in the period from 8–24 h, and exhibits significantly attenuated oxidative stress and liver injury. In summary, we reveal the important role of TrxA in the pathogenesis of SS2.

Evaluation and Comparation of a Novel Surgical Technique and Hemivertebra Resection to the Correction of Congenital Cervical Scoliosis in Lower Cervical and Cervicothoracic Spine

Shuo Cao,Xin Chen,Shengfa Pan,Yinze Diao,Yanbin Zhao,Tian Xia,Weishi Li,Feifei Zhou,Yu Sun 대한척추신경외과학회 2022 Neurospine Vol.19 No.4

Objective: To report concave-side distraction technique to treat congenital cervical scoliosis in lower cervical and cervicothoracic spine. To evaluate and compare clinical and radiographic results of this procedure with classic hemivertebra resection procedure. Methods: This study reviewed 29 patients in last 13 years. These patients were divided into convex-side resection group (group R) and concave-side distraction group (group D). Radiographic assessment was based on parameter changes preoperatively, postoperatively and at last follow-up. Demographic data, surgical data and complications were also evaluated and compared between the 2 groups. Results: In group R, mean age was 8.9 ± 3.3 years and follow-up was 46 ± 18 months. Operation time and blood loss averaged 500 ± 100 minutes, 703 ± 367 mL. In group D, mean age was 9.9 ± 2.8 years and follow-up was 34 ± 14 months. Operation time and blood loss averaged 501 ± 112 minutes, 374 ± 181 mL. Structural Cobb angle was corrected from 29.4° ± 12.5° to 5.3° (2.1°–18.1°) (p = 0.001) and 33.7° ± 14.1° to 12.8° ± 11.4° (p < 0.001) in groups R and D. Compensatory Cobb angle had a spontaneous correction rate of 59.6% (40.0%–80.8%) and 59.7% ± 23.0% in groups R and D. Mandibular incline, clavicle angle and spine coronal balance were significantly improved at last follow-up in both groups. All correction rates were not statistically different between groups. However, group D had significant less blood loss (p < 0.001) and operation time (p = 0.004) per vertebra than group R. Seven patients developed C5 nerve root palsy and recovered by 6 months of follow-up. Conclusion: Both surgical procedures are safe and effective in correcting congenital cervical scoliosis. But concave-side distraction technique has less blood loss and time-consuming during surgery, which provides a better option for the treatment of congenital cervical scoliosis.

Selection of inspection path optimization scheme based on analytic hierarchy process and inspection experimental study

Shuo Zhang,Jiantao Yao,Ruochao Wang,Yu Tian,Jiaxin Wang,Yongsheng Zhao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.1

This paper mainly studies the optimization of the patrol route of a fire inspection robot, based on analytic hierarchy process (AHP) and fire source detection. First, the A* algorithm is improved based on two aspects of the heuristic function and of the obstacle boundary setting, while the suboptimal path is obtained in MATLAB. Next, the path planned according to the improved A* algorithm is smoothed and optimized, by means of gradient descent method, Bezier curve and B-spline curve, while the index parameters are optimized by means of MATLAB simulation. In view of the simulation results, the trajectory optimization performance index evaluation system, established by five decision criteria, including running time, path length, ride comfort, no-collision effect and quadratic optimization space, is put forward. The three kinds of optimization methods are analyzed qualitatively and quantitatively, and the results show that, in the total hierarchical ranking, the B-spline curve trajectory optimization scheme has the largest weight and is more important than the other two schemes. Finally, the superiority of B-spline curve is verified experimentally.

Photosynthetic inhibition and oxidative stress to the toxic Phaeocystis globosa caused by a diketopiperazine isolated from products of algicidal bacterium metabolism

Shuo Tan,Xiaoli Hu,Pinghe Yin,Ling Zhao 한국미생물학회 2016 The journal of microbiology Vol.54 No.5

Algicidal bacteria have been turned out to be available for inhibiting Phaeocystis globosa which frequently caused harmful algal blooms and threatened to economic development and ecological balance. A marine bacterium Bacillus sp. Ts-12 exhibited significant algicidal activity against P. globosa by indirect attack. In present study, an algicidal compound was isolated by silica gel column, Sephadex G-15 column and HPLC, further identified as hexahydropyrrolo[1,2-a]pyrazine- 1,4-dione, cyclo-(Pro-Gly), by GC-MS and 1H-NMR. Cyclo-(Pro-Gly) significantly increased the level of reactive oxygen species (ROS) within P. globosa cells, further activating the enzymatic and non-enzymatic antioxidant systems, including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and ascorbic acid (AsA). The increase in methane dicarboxylic aldehyde (MDA) content showed that the surplus ROS induced lipid peroxidation on membrane system. Transmission electron microscope (TEM) and flow cytometry (FCM) analysis revealed that cyclo-(Pro-Gly) caused reduction of Chl-a content, destruction of cell membrane integrity, chloroplasts and nuclear structure. Real-time PCR assay showed that the transcriptions of photosynthesis related genes (psbA, psbD, rbcL) were significantly inhibited. This study indicated that cyclo-(Pro-Gly) from marine Bacillus sp. Ts-12 exerted photosynthetic inhibition and oxidative stress to P. globosa and eventually led to the algal cells lysis. This algicidal compound might be potential bio-agent for controlling P. globosa red tide.

Dynamic Visual Performance of LED with Different Color Temperature

Zhao Jiandong,Ma Shuo 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6

In order to research the dynamic visual performance of LED in mesopic vision conditions, based on the visual performance method, the software systems were developed to set different test parameters. And the hardware systems were used to collect the reaction time of testers. Color temperature of LED, ambient luminance, luminance contrast and target speed were selected as the experimental variables, and each variable had three variances. 60 sets of experiments were carried out by 20 testers. The experiment results show that reaction time of high color temperature LED is shorter than the other two. And reaction time decreases with the increasing of the ambient luminance and luminance contrast. And target speed has significant influence on visual performance in the speed range of 60km/h to 80km/h.