Sesamin induces A549 cell mitophagy and mitochondrial apoptosis via a reactive oxygen species-mediated reduction in mitochondrial membrane potential

Shasha Yang,Xiangdan Li,Haowen Dou,Yulai Hu,Chengri Che,Dongyuan Xu 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.3

Sesamin, a lipid-soluble lignin originally isolated from sesame seeds, which induces cancer cell apoptosis and autophagy. In the present study, has been reported that sesamin induces apoptosis via several pathways in human lung cancer cells. However, whether mitophagy is involved in sesamin induced lung cancer cell apotosis remains unclear. This study, the anticancer activity of sesamin in lung cancer was studied by reactive oxygen species (ROS) and mitophagy. A549 cells were treated with sesamin, and cell viability, migration ability, and cell cycle were assessed using the CCK8 assay, scratch-wound test, and flow cytometry, respectively. ROS levels, mitochondrial membrane potential, and apoptosis were examined by flow cytometric detection of DCFH-DA fluorescence and by using JC-1 and TUNEL assays. The results indicated that sesamin treatment inhibited the cell viability and migration ability of A549 cells and induced G0/G1 phase arrest. Furthermore, sesamin induced an increase in ROS levels, a reduction in mitochondrial membrane potential, and apoptosis accompanied by an increase in cleaved caspase-3 and cleaved caspase-9. Additionally, sesamin triggered mitophagy and increased the expression of PINK1 and translocation of Parkin from the cytoplasm to the mitochondria. However, the antioxidant N-acetyl-L-cysteine clearly reduced the oxidative stress and mitophagy induced by sesamin. Furthermore, we found that cyclosporine A (an inhibitor of mitophagy) decreased the inhibitory effect of sesamin on A549 cell viability. Collectively, our data indicate that sesamin exerts lethal effects on lung cancer cells through the induction of ROS-mediated mitophagy and mitochondrial apoptosis.

Investigation on Microstructure, Hardness and Wear Resistance of Electron Beam Wire-Feeding Deposited Inconel 718 Alloy Coatings

Dongheng Xu,Han Wang,Xuewei Tao,Zhengjun Yao,Shasha Zhang,Moliar Oleksander 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5

The Inconel 718 (IN718) alloy coatings were successfully fabricated using electron beam wire-feeding deposition technology.The macrostructure, microstructure and elemental analysis of the deposited coatings were characterized by OM, SEMand EDS. Moreover, the hardness and wear resistance were also investigated experimentally. The results showed that thecross section of the deposited coatings can be divided into three different regions: clad zone (CZ), fusion zone (FZ) and heataffected zone. Equiaxed dendrites appeared in the CZ while columnar dendrites occurred in the FZ, and discrete fine Lavesphase particles were formed under low beam current while continuous coarse Laves phase particles were found under highbeam current. The EDS results showed that the degree of Nb segregation in FZ is higher than that in CZ. More importantly,the microstructure coarsened and the degree of Nb segregation increased with the increase of beam current. The depositedcoating under the lowest beam current (10 mA) has the highest hardness (263 HV0.2) and the minimum specific wear rate(3.95391 × 10−15 m3/Nm), which is corresponding to the fine microstructure, discrete Laves phase particles and low degreeof Nb segregation under low beam current.

Association of a novel antisense lncRNA TP73-AS1 polymorphisms and expression with colorectal cancer susceptibility and prognosis

Fan Jiayao,Xu Huiqing,Liu Bing,Jing Fangyuan,He Qingfang,Zheng Shasha,Shi Haining,Jiao Lefei,Fan Chunhong 한국유전학회 2022 Genes & Genomics Vol.44 No.7

Background: TP73-AS1 is a novel antisense long noncoding RNA and plays an important role in cell proliferation and cancer development. However, the link between TP73-AS1 and colorectal cancer (CRC) has not yet been reported. Objective: To explore the association of genetic variants in TP73-AS1 and its expression with CRC susceptibility and prognosis. Methods: A case-control study (including 507 CRC cases and 503 controls) and bioinformatics analysis were conducted. Results: rs9800 polymorphism was significantly related to higher risk in CRC [adjusted odds ratio (AOR) = 1.33, 95% confidence interval (CI) = 1.02-1.75, P = 0.034 in heterozygote codominant model]. There was no difference between TP73-AS1 polymorphisms and different tumor node metastasis (TNM) stages in the adjusted model. Moreover, TP73-AS1 expression level was positively related to different TNM stages. After adjusted for age, gender and TNM, higher TP73-AS1 expression levels were related to shorter recurrence-free survival time [hazard ratio (HR) = 1.66, 95% CI = 1.02-2.71, P = 0.043]. Conclusion: TP73-AS1 polymorphisms and expression may be associated with susceptibility and prognosis of CRC.

Gradient folding metasurfaces with simultaneous phase and amplitude modulation

Zibin Lin,Weikai Xu,Wei Wang,Shasha Yuan 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.12

An elastic metasurface with a gradient index of functional units, namely gradient folding metasurface (GFMS), is proposed to simultaneously manipulate the transmission phase and amplitude of flexural waves. By varying the gradient index common ratio (CR) between each adjacent unit, the GFMS was found to cover an entire 2π span of phase shift. At the same time, the amplitude of the transmitted flexural wave can also be modulated. Based on the generalized Snell's law, two different cases of the wave focusing effect are realized, which illustrate CR dependent wave propagation mode from symmetric to asymmetric and verify the ability of the proposed metasurface to manipulate complete elastic wave through simultaneous phase and amplitude modulation. This study expands phase-amplitude modulation from acoustics to elastic waves and provides more degrees of freedom in elastic metasurface designing.

Synthesis of CdS Nanocrystals with Different Shapes via a Colloidal Method

Jie Bai,Changsong Liu,Jinzhong Niu,Hongzhe Wang,Shasha Xu,Huaibin Shen,Lin Song Li 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

Size- and shape-controlled monodisperse wurtzite structured CdS nanorods have been successfully synthesized using a facile solution-based colloidal method. Depending on the control of injection/growth temperatures and the variation of Cd-to-S molar ratios, the morphology of the CdS nanocrystals (NCs) can be adjusted into bullet-like, rod-like, and dot-like shapes. X-ray diffraction (XRD), transition electron microscopy (TEM), and absorption spectroscopy were used to characterize the structure, morphology, and optical properties of assynthesized CdS NCs. It was found that uniform CdS nanorods could be successfully synthesized when the injection and growth temperatures were very high (> 360 °C). The aspect ratios of different shaped (bullet-like or rod-like) CdS NCs could be controlled by simply adjusting the molar ratios between Cd and S.

miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells

Xuehua Liu,Mengmeng Li,Yuzhu Peng,Xiaoshan Hu,Jing Xu,Shasha Zhu,Zhangbin Yu,Shuping Han 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that suppress protein expression by binding to the 3′ untranslated regions of their target genes. Many studies have shown that miRNAs have important roles in congenital heart diseases (CHDs) by regulating gene expression and signaling pathways. We previously found that miR-30c was highly expressed in the heart tissues of aborted embryos with ventricular septal defects. Therefore, this study aimed to explore the effects of miR-30c in CHDs. miR-30c was overexpressed or knocked down in P19 cells, a myocardial cell model that is widely used to study cardiogenesis. We found that miR-30c overexpression not only increased cell proliferation by promoting cell entry into S phase but also suppressed cell apoptosis. In addition, we found that miR-30c inhibited dimethyl sulfoxide-induced differentiation of P19 cells. miR-30c knockdown, in contrast, inhibited cell proliferation and increased apoptosis and differentiation. The Sonic hedgehog (Shh) signaling pathway is essential for normal embryonic development. Western blotting and luciferase assays revealed that Gli2, a transcriptional factor that has essential roles in the Shh signaling pathway, was a potential target gene of miR-30c. Ptch1, another important player in the Shh signaling pathway and a transcriptional target of Gli2, was downregulated by miR-30c overexpression and upregulated by miR-30c knockdown. Collectively, our study revealed that miR-30c suppressed P19 cell differentiation by inhibiting the Shh signaling pathway and altered the balance between cell proliferation and apoptosis, which may result in embryonic cardiac malfunctions.

Synthesis of CdS Nanocrystals with Different Shapes via a Colloidal Method

Bai, Jie,Liu, Changsong,Niu, Jinzhong,Wang, Hongzhe,Xu, Shasha,Shen, Huaibin,Li, Lin Song Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

Size- and shape-controlled monodisperse wurtzite structured CdS nanorods have been successfully synthesized using a facile solution-based colloidal method. Depending on the control of injection/growth temperatures and the variation of Cd-to-S molar ratios, the morphology of the CdS nanocrystals (NCs) can be adjusted into bullet-like, rod-like, and dot-like shapes. X-ray diffraction (XRD), transition electron microscopy (TEM), and absorption spectroscopy were used to characterize the structure, morphology, and optical properties of as-synthesized CdS NCs. It was found that uniform CdS nanorods could be successfully synthesized when the injection and growth temperatures were very high (> $360^{\circ}C$). The aspect ratios of different shaped (bullet-like or rod-like) CdS NCs could be controlled by simply adjusting the molar ratios between Cd and S.

Different Characteristics of Psychological and Sleep Symptoms Across Social Media Addiction and Internet Gaming Disorder in Chinese Adolescents- A Network Analysis

Wanling Zhang,Liwen Jiang,Minglan Yu,Rong Ma,Tingting Wang,Xuemei Liang,Rongfang He,Chun Xu,Shasha Hu,Youguo Tan,Kezhi Liu,Bo Xiang 대한신경정신의학회 2024 PSYCHIATRY INVESTIGATION Vol.21 No.7

Objective Previous research has explored a variety of mental disorders associated with Internet Gaming Disoder (IGD) and Social Media Addiction (SMA). To date, few studies focused on the network characteristics and investigated mood and sleep symptoms across SMA and IGD of adolescence at a group-specific level. This study aims to identify different characteristics of IGD and SMA and further determine the group-specific psychopathology process among adolescents. Methods We conducted a cross-sectional study to recruit a cohort of 7,246 adolescents who were scored passing the cutoff point of Internet Gaming Disorder Scale-Short Form and Bergen Social Media Addiction Scale, as grouped in IGD and SMA, or otherwise into the control group. Patient Health Questionnaire-9, Generalized Anxiety Disorder 7-item, and Pittsburgh Sleep Quality Index were assessed for the current study, and all assessed items were investigated using network analysis. Results Based on the analytical procedure, the participants were divided into three groups, the IGD group (n=789), SMA group (n=713) and control group (n=5,744). The edge weight bootstrapping analysis shows that different groups of networks reach certain accuracy, and the network structures of the three groups are statistically different (pcontrol-IGD=0.004, pcontrol-SMA<0.001, pIGD-SMA<0.001). The core symptom of SMA is “feeling down, depressed, or hopeless”, while IGD is “feeling tired or having little energy”. Conclusion Although IGD and SMA are both subtypes of internet addiction, the psychopathology processes of IGD and SMA are different. When dealing with IGD and SMA, different symptoms should be addressed. Psychiatry Investig 2024;21(7):782-791