Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization

Moon, Seyoung,Kim, Dong Jun,Kim, Kyujung,Kim, Donghyun,Lee, Hosub,Lee, Kangtaek,Haam, Seungjoo The Optical Society 2010 Applied optics Vol.49 No.3

<P>We have investigated surface-enhanced plasmon resonance detection of DNA hybridization. Surface enhancement was based on the excitation of localized surface plasmon using subwavelength nanogratings, at a 300 nm period, coated with 24-mer ssDNA oligonucleotide, while optical signatures of DNA were amplified at the same time by gold nanoparticles conjugated with complementary ssDNA strands. When using nanoparticles of different sizes, maximum sensitivity enhancement, of more than 18 times, was obtained with nanoparticles of 20 nm diameter. This enhancement is mainly due to nanoparticle-associated signal amplification. Additional surface enhancement boosted the detection sensitivity by 57%. We have also confirmed the sensitivity enhancement to be linearly related to nanoparticle volume.</P>

Fitting-based determination of an effective medium of a metallic periodic structure and application to photonic crystals.

Moon, Seyoung,Kim, Donghyun Optical Society of America 2006 Journal of the Optical Society of America A Vol.23 No.1

<P>Effective permittivities of a metallic periodic structure for which the second-order effective-medium theory does not yield correct results were obtained by numerically fitting to rigorous-coupled-wave analysis (RCWA). The calculated effective medium showed good agreement with RCWA and minimal deviation in the long-wavelength limit with variation in angle of incidence, grating depth, superstrate, and fill factor. In terms of the standard deviation, the effective medium was least affected by the change in grating depths. The calculated effective permittivities were applied to a three-dimensional metallic photonic-crystal structure and produced a photonic bandgap that is consistent with published experimental data.</P>

의용소방대원들의 재난에 대한 인식과 만족이 자원봉사활동 지속성에 미치는 영향

Seyoung Lim,Hyeonji Lee,Miyoung Choi,Jeonghyeon Hwang,Munui Kim,Taeyoung Moon 한국재난정보학회 2015 한국재난정보학회 논문집 Vol.11 No.2

본 연구의 목적은 의용소방대원들의 재난에 대한 인식이 자원봉사활동 지속성에 미치는 영향을 규명하기 위하여 강원도 시⦁군에 거주하고 의용소방대원으로 활동하고 있는 의용 소방대원 남녀 163명을 대상으로 조사하였다. 수집된 자료는 Windows용 20.0을 사용하여 빈도분석, 상관관계 및 다중회귀분석을 실시하였다. 모든 통계분석은 유의수준 p<.05로 설 정하였다. 결과는 다음과 같다. 첫째, 의용소방대원들의 재난에 대한 인식, 만족, 자원봉사활 동 지속성 간의 상관관계는 재난대책과 만족 및 참여정도에서 부적 상관을 보였고, 재난훈 련, 재난준비, 지역재난, 지속시간, 지속의지 등은 정적 상관관계를 나타났다. 둘째, 의용소 방대원들의 재난에 대한 인식도에서는 재난대책을 부분적으로 알고 있다고 가장 많이 답했 으며, 재난훈련 교육경험 및 중증도 분류에 질문에 대체적으로 있었음으로 가장 높았다. 또한, 재난 시 비상연락체계 및 비상근무시간표에 대한 질문에 대체적으로 잘 알고 있는 것 으로 나타났고, 인적 재난발생에 대한 질문에 대체적으로 보통이 가장 높았다. 셋째, 의용소 방대들의 만족도가 자원봉사활동 지속성에 미치는 영향으로 만족도의 하위요인인 중 경험 욕구, 사회접촉, 사회인정이 지속시간에 영향을 미치는 것으로 나타났고, 지속의지에는 사회 접촉, 성취욕구, 지속정도에는 경험욕구 및 성취욕구만 영향이 미치는 것으로 나타났다. The purpose of this study was to examine the influence of the disaster perception and satisfaction level of volunteer fire-fighters on the continuity of their volunteering. The 163 subjects in this study were selected from male and female volunteer fire-fighters who resided in urban and rural regions in Gangwon Province. After a this survey was conducted, the collected data were analyzed by a statistical package SPSS WIN 20.0, and frequency analysis, correlation analysis and multiple regression analysis were made. The level of statistical significance was all set at p<.05. The findings of the study were as follows: First, as for the correlation of disaster perception, satisfaction and volunteering continuity, perception of disaster countermeasures, satisfaction and the level of participation were negatively correlated with one another, and there was a positive correlation among disaster training, disaster preparation, regional disaster, the period of volunteering, and will of persistent volunteering. Second, as for perception of disaster, the volunteer fire-fighters were asked a question about disaster countermeasures, and the largest group replied they were partially aware of the countermeasures. Concerning questions about disaster training/education experience and triage, the biggest group replied they underwent the training and knew about triage on the whole. Regarding questions on the emergency contact system and emergency work schedule, they knew about the two in general. As to a question on the occurrence of human disaster, the greatest group answered that they knew about it yet not well. Third, in regard to the impact of satisfaction level on volunteering continuity, the period of volunteering was affected by needs for experience, social contact and social recognition among the subfactors of satisfaction level, and will of persistent volunteering was under the influence of social contact and achievement needs. The level of persistent volunteering was affected only by needs for experience and achievement needs.

Fairness scale development in the tourism domain

SeYoung Park,Won Seok Lee,Joonho Moon 한국관광연구학회 2019 관광연구저널 Vol.33 No.4

의용소방대원들의 재난에 대한 인식과 만족이 자원봉사활동 지속성에 미치는 영향

Lim, Seyoung,Lee, Hyeonji,Choi, Miyoung,Hwang, Jeonghyeon,Kim, Munui,Moon, Taeyoung 한국재난정보학회 2015 한국재난정보학회 논문집 Vol.11 No.2

본 연구의 목적은 의용소방대원들의 재난에 대한 인식이 자원봉사활동 지속성에 미치는 영향을 규명하기 위하여 강원도 시 군에 거주하고 의용소방대원으로 활동하고 있는 의용소방대원 남녀 163명을 대상으로 조사하였다. 수집된 자료는 Windows용 20.0을 사용하여 빈도분석, 상관관계 및 다중회귀분석을 실시하였다. 모든 통계분석은 유의수준 p<.05로 설정하였다. 결과는 다음과 같다. 첫째, 의용소방대원들의 재난에 대한 인식, 만족, 자원봉사활동 지속성 간의 상관관계는 재난대책과 만족 및 참여정도에서 부적 상관을 보였고, 재난훈련, 재난준비, 지역재난, 지속시간, 지속의지 등은 정적 상관관계를 나타났다. 둘째, 의용소방대원들의 재난에 대한 인식도에서는 재난대책을 부분적으로 알고 있다고 가장 많이 답했으며, 재난훈련 교육경험 및 중증도 분류에 질문에 대체적으로 있었음으로 가장 높았다. 또한, 재난 시 비상연락체계 및 비상근무시간표에 대한 질문에 대체적으로 잘 알고 있는 것으로 나타났고, 인적 재난발생에 대한 질문에 대체적으로 보통이 가장 높았다. 셋째, 의용소방대들의 만족도가 자원봉사활동 지속성에 미치는 영향으로 만족도의 하위요인인 중 경험욕구, 사회접촉, 사회인정이 지속시간에 영향을 미치는 것으로 나타났고, 지속의지에는 사회접촉, 성취욕구, 지속정도에는 경험욕구 및 성취욕구만 영향이 미치는 것으로 나타났다. The purpose of this study was to examine the influence of the disaster perception and satisfaction level of volunteer fire-fighters on the continuity of their volunteering. The 163 subjects in this study were selected from male and female volunteer fire-fighters who resided in urban and rural regions in Gangwon Province. After a this survey was conducted, the collected data were analyzed by a statistical package SPSS WIN 20.0, and frequency analysis, correlation analysis and multiple regression analysis were made. The level of statistical significance was all set at p<.05. The findings of the study were as follows: First, as for the correlation of disaster perception, satisfaction and volunteering continuity, perception of disaster countermeasures, satisfaction and the level of participation were negatively correlated with one another, and there was a positive correlation among disaster training, disaster preparation, regional disaster, the period of volunteering, and will of persistent volunteering. Second, as for perception of disaster, the volunteer fire-fighters were asked a question about disaster countermeasures, and the largest group replied they were partially aware of the countermeasures. Concerning questions about disaster training/education experience and triage, the biggest group replied they underwent the training and knew about triage on the whole. Regarding questions on the emergency contact system and emergency work schedule, they knew about the two in general. As to a question on the occurrence of human disaster, the greatest group answered that they knew about it yet not well. Third, in regard to the impact of satisfaction level on volunteering continuity, the period of volunteering was affected by needs for experience, social contact and social recognition among the subfactors of satisfaction level, and will of persistent volunteering was under the influence of social contact and achievement needs. The level of persistent volunteering was affected only by needs for experience and achievement needs.

Clinical outcomes of patients with resectable pancreatic acinar cell carcinoma

Seo, Seyoung,Yoo, Changhoon,Kim, Kyu‐,poy,Ryoo, Baek‐,Yeol,Chang, Heung‐,Moon,Hong, Seung‐,Mo,Lee, Jae Hoon,Song, Ki Byung,Hwang, Dae Wook,Kim, Ki‐,Hun,Hwang, Shin,Kim, S Wiley Publishing Asia Pty Ltd 2017 Journal of digestive diseases Vol.18 No.8

<P><B>OBJECTIVE</B></P><P>Given the rarity of the disease, the post‐resection clinical course of localized pancreatic acinar cell carcinoma (ACC) is largely unknown. Therefore, we aimed to analyze the outcomes of patients with localized pancreatic ACC who underwent curative surgical resection.</P><P><B>METHODS</B></P><P>We retrospectively analyzed the outcomes of 20 patients with resectable pancreatic ACC who underwent surgery.</P><P><B>RESULTS</B></P><P>Altogether 20 patients were included in the study, with a median age of 57 years and a male predominance. There were eight pure ACC, 10 mixed acinar–neuroendocrine carcinomas and two mixed acinar–ductal adenocarcinomas. Among the 15 patients who were staged histologically, 3, 8 and 4 were at stages IB, IIA and IIB, respectively. Eleven patients received adjuvant chemotherapy (5‐fluorouracil‐based [<I>n</I> = 9]; gemcitabine [<I>n</I> = 1]; etoposide plus cisplatin [<I>n</I> = 1]). In a median follow‐up period of 27.1 months, disease recurred in 10 patients, most commonly in the liver (90%). Median recurrence‐free survival and overall survival were 16.9 months and 75.0 months, respectively. Elevation of cancer antigen 19‐9 (CA19‐9), lymph node metastasis and neural invasion were significantly associated with poor overall survival (<I>P</I> = 0.007, <I>P</I> = 0.027 and <I>P</I> = 0.016, respectively).</P><P><B>CONCLUSIONS</B></P><P>Compared with ductal adenocarcinoma, resectable pancreatic ACC has a favorable prognosis after surgery. Considering that distant metastasis is the most common pattern of recurrence, further studies are necessary to define the role of adjuvant chemotherapy for improving survival outcomes.</P>

NB-IoT 시스템에서의 채널 추정 기법

조세영(Seyoung Cho),장갑석(Kapseok Chang),이문식(Moon-Sik Lee) 한국통신학회 2018 한국통신학회 학술대회논문집 Vol.2018 No.1

Effect of coupled graphene oxide on the sensitivity of surface plasmon resonance detection

Ryu, Yeonsoo,Moon, Seyoung,Oh, Youngjin,Kim, Yonghwi,Lee, Taewoong,Kim, Dong Ha,Kim, Donghyun The Optical Society 2014 Applied Optics Vol.53 No.7

<P>We investigated graphene-oxide-(GO-) coupled surface plasmon resonance (SPR) detection sensitivity for sandwiched antigen-antibody interaction between human and antihuman immunoglobulin G molecules. GO was prepared in a Langmuir-Blodgett solution on gold and dielectric surfaces. Theoretical and experimental data suggest that an increased dielectric spacer thickness reduces resonance shifts for GO-coupled SPR detection as dielectric properties of GO appear to prevail. In general, a metal-enhanced structure was shown to provide a larger resonance shift by plasmonic field enhancement. The far-field properties were described in terms of near-field overlap. The peak resonance shift that was obtained with GO-coupled SPR detection was enhanced to 113% of the resonance shift obtained by conventional thin-film-based SPR detection and may further be improved by GO stacking.</P>

Simple one-pot synthesis of Rh–Fe₃O₄ heterodimer nanocrystals and their applications to a magnetically recyclable catalyst for efficient and selective reduction of nitroarenes and alkenes

Jang, Youngjin,Kim, Seyoung,Jun, Samuel Woojoo,Kim, Byung Hyo,Hwang, Sunhwan,Song, In Kyu,Kim, B. Moon,Hyeon, Taeghwan Royal Society of Chemistry 2011 Chemical communications Vol.47 No.12

<P>A simple synthesis of Rh–Fe<SUB>3</SUB>O<SUB>4</SUB> heterodimer nanocrystals was achieved by controlled one-pot thermolysis. The nanocrystals exhibited excellent activities for the selective reduction of nitroarenes and alkenes. Furthermore the nanocrystal catalyst could be easily separated by a magnet, and recycled eight times without losing the catalytic activity.</P> <P>Graphic Abstract</P><P>Rh–Fe<SUB>3</SUB>O<SUB>4</SUB> heterodimer nanocrystals, made by controlled one-pot thermolysis, show excellent activities for reduction of nitroarenes and alkenes. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0cc04816j'> </P>

Development of ERE/DRE-dual CALUX Bioassays System for Monitoring Estrogen- and Dioxin-like Persistent Organic Pollutants

권미선,이효진,김선희,Seyoung Lim,JONG MOON PARK,Sung Ho Ryu,Jae-Ho Yang,Pann-Ghill Suh 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

Estrogen- and dioxin-like responsive element mediated Chemically Activated LUciferine gene eXpression (ERE/DRE-dual CALUX) was utilized to develop dualbioassays for simultaneously determining estrogen and dioxin levels. The devised ERE/DRE-dual CALUX bioassay system, was applied to the analysis of 103 estrogen and dioxin-related isomers, PAHs, PCBs, and pesticides. The nonylphenol highly induced estrogen-like activity (9.95 times) while benzo(k)fluoranthene, chrysene (zonerefined),and 3,3',4,4',5-pentachlorobiphenyl, highly induced dioxin-like activity (32.23, 42.39, and 34.47 times) respectively as compared with the untreated control. To confirm the validity of the devised ERE/DRE-dual CALUX bioassay system, we compared this bioassay with high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) to assess five air samples taken in Korea. The correlation coefficient between the ERE/DRE-dual CALUX bioassay system and HRGC/HRMS method showed a good relationship (r2 = 0.92). This study indicates that ERE/DRE-dual CALUX is a simple, high-throughput, valid,and inexpensive bioassay method for screening large numbers of environmental samples.