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Box-Wilson 계획법에 의한 沈降炭酸칼슘의 製造條件에 關한 硏究
김영윤,신화우,최광식,안세민,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-
Medicinal Precipitated Clacium Carbonate is prepared by the interaction of Calcium Chloride and Ammonium Carbonate Solutions in this study. It has been known that the particle size of Precipitated Calcium Carbonate is greatly affected by the concentration, temperature, and moli-ratio of reactant solutions as well as the temperature at which the precipitate is dried and the temperature of washing water. The purpose of this study is to determine the optimum preparation Condition of medicinal Precipitated Calcium Carbonate. A randomized complete block design wuggested by G.E.P. Box and K.B.Wilson was for this purpose. The optimum preparation condition of light and fine precipitated Calcium Carbonate obtained from this study is as follows : 1) The reacting temperature range is 25∼45℃ 2) The concentration range of reactant solutions is 10∼30% 3) The optimum mole-ratio (CaCl/(NH) CO) is 1.0∼2.0. 4) The drying temperature range is 60∼80℃. 5) Temperature range of washing water is 25∼60℃.
Cho, Seong-Keun,Kim, Jae-Hwan,Park, Jong-Yi,Choi, Yun-Jung,Bang, Jae-Il,Hwang, Kyu-Chan,Cho, Eun-Jeong,Sohn, Sea-Hwan,Uhm, Sang Jun,Koo, Deog-Bon,Lee, Kyung-Kwang,Kim, Teoan,Kim, Jin-Hoi Wiley-Liss,Inc 2007 Developmental dynamics Vol.236 No.12
<P>Somatic cell nuclear transfer (scNT) is a useful way to create cloned animals. However, scNT clones exhibit high levels of phenotypic instability. This instability may be due to epigenetic reprogramming and/or genomic damage in the donor cells. To test this, we produced transgenic pig fibroblasts harboring the truncated human thrombopoietin (hTPO) gene and used them as donor cells in scNT to produce first-generation (G1) cloned piglets. In this study, 2,818 scNT embryos were transferred to 11 recipients and five G1 piglets were obtained. Among them, a clone had a dimorphic facial appearance with severe hypertelorism and a broad prominent nasal bridge. The other clones looked normal. Second-generation (G2) scNT piglets were then produced using ear cells from a G1 piglet that had an abnormal nose phenotype. We reasoned that, if the phenotypic abnormality of the G1 clone was not present in the G2 and third-generation (G3) clones, or was absent in the G2 clones but reappeared in the G3 clones, the phenotypic instability of the G1 clone could be attributed to faulty epigenetic reprogramming rather than to inherent/accidental genomic damage to the donor cells. Blastocyst rates, cell numbers in blastocyst, pregnancy rates, term placenta weight and ponderal index, and birth weight between G1 and G2 clones did not differ, but were significantly (P < 0.05) lower than control age- and sex-matched piglets. Next, we analyzed global methylation changes during development of the preimplantation embryos reconstructed by donor cells used for the production of G1 and G2 clones and could not find any significant differences in the methylation patterns between G1 and G2 clones. Indeed, we failed to detect the phenotypic abnormality in the G2 and G3 clones. Thus, the phenotypic abnormality of the G1 clone is likely to be due to epigenetic dysregulation. Additional observations then suggested that expression of the hTPO gene in the transgenic clones did not appear to be the cause of the phenotypic abnormality in the G1 clones and that the abnormality was acquired by only a few of the G1 clone's cells during its gestational development. Developmental Dynamics 236:3369–3382, 2007. © 2007 Wiley-Liss, Inc.</P>
家蠶에서 分離된 새로운 微胞子蟲(S_85)의 特性 및 分類學籍 位置(1) : 胞子의 微細構造
趙世衍,孫海龍,林種聲 한국잠사학회 1989 한국잠사곤충학회지 Vol.31 No.2
A new microsporidia (S85) was isolated from the silkworm, Bombyx mori L. in Suwon, Korea in 1985. The electron microscopic observation of the fine structure of the spore was studied for the characterization of the new microsporidia and its taxonomic position. The sporewall was composed of three different electron dense layers and the spore surface was rouph and wrinkled. The polaroplast has two parts which differed in the arrangement of the lamellae. The polarfilament was isofilar with thirteen coils and it was consisted of eight concentric layers of different electron density. The spore contained a single nucleus and the pansporoblast appeared as a thin balloon-like structure surrounding the spore. The membrane was generally smooth and shown occationally wrinkled.
천식 환자에서 증상의 정도에 따른 IL-4 유전자 다형에 관한 연구
강세용 ( Sea Yong Kang ),심재정 ( Jae Jeong Shim ),조재연 ( Jae Yun Cho ),권영환 ( Young Hwan Kwon ),이승룡 ( Seung Yong Lee ),김제형 ( Je Hyeong Kim ),이상엽 ( Sang Youb Lee ),이소라 ( So Ra Lee ),한선애 ( Seon Ae Han ),김한겸 ( 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.3
蔡洙根,朴大陽,趙世衍,長大洙 한국잠사학회 1986 한국잠사곤충학회지 Vol.28 No.1
Silkworms have been found cross infected with other microsporidia of insects in mulberry trees, forest and fruit gardens. Even the unidentified microsporidian species were not seriously pathogenic to silkworms, the silkworm egg producers lose their profit because of the elimination of eggs laid from moths which are infected with any kind of microsporidian species. Recently, the microsporidian cross infection to silkworm is in tendency of increase and the authors have investigated the field insects to examine the microsporidia. The number of species of insects infected with microsporidia was 10 and they were Boellcherisca peregrina (Rebineau-Desvoidy), Apis melifera Linnacus, Artogenia rapae Linnacus, Tipula aino Alexander, Altica caeruleseens (Baly), Anomela daimiana Harold, Eilema griseola (Hubner), Rbalbistylun speciosum Uller, Anisodactylus signatus Illiger, Oulema oryzae (Kuwayama). From the Boettcherisca peregrina (Robineau-Desvoidy), three different species of microsporidia were isolated and the microsporidia isolated from Boettcherisca peregrina (Robineau-Desvoidy), Apis melifera Linnacus, Artogenia rapae Linnacus donot have infectivity to inflectivity to silkworm larvae, Bombyx mori L.
자료(資料) : 1984년도(年度) 추잠기(秋蠶期) 잠병발생(蠶病發生) 실태조사(實態調査)
채수군 ( Soo Kun Chae ),박대양 ( Dae Yang Park ),조세연 ( Sea Yun Cho ),이길재 ( Kil Jae Lee ) 한국잠사학회 1984 한국잠사곤충학회지 Vol.26 No.2
전국 621개 양잠시범부락의 84가을 누에병 발생율과 그 원인을 조사한 결과는 다음과 같다. 1. 누에 사육 기간 중 누에병 발생율은 약 17%이었다. 2. 누에병 종류별 발생율은 농병(38.5%), 경화병(20.3%), 연화병(17.9%) 및 쉬파리병(9.4%)의 순이었다. 3. 누에병은 종잠기보다 장잠기 이후에 주로 발생하였다. 4. 누에병 발생의 주원인은 기상환경불량, 농약피해 및 소독불량이었고, 엽질불량, 사육기술부족 및 공해는 큰 문제가 되지 않았다. The survey of silkworm disease occurrence in autumn rearing season, 1984, was carried out and the results are as follows. 1. The percentage of diseased larvae in the current season was about 17%. 2. Among silkworm diseases, grassarie was predominant, as it took 38.5% of total diseased larvae, followed by muscardine which took 20.3% and flacherie was taken by 11.9% 3. The infection of silkworm larvae with pathogens occurred more at grown larva stages than at young larva stages. 4. The loss of cocoon yield was mainly caused by unsuitable climate conditions and agricultural insecticides applied in the rice paddy fields and fruit gardens near mulberry fields. The cocoon production also was reduced by the failure of silkworm disease control due to the incomplete disinfection of rearing rooms and tools.