급성 충수염 - 2006년 대한소아외과학회회원 대상 전국조사 -

이석구,김대연,김상윤,김성철,김신곤,김우기,김인구,김재억,김재천,박귀원,박우현,서정민,송영택,오정탁,이남혁,이두선,이명덕,이성철,장수일,전용순,정상영,정성은,정을삼,정풍만,Lee, Suk-Koo,Kim, D.Y.,Kim, S.Y.,Kim, S.C.,Kim, S.G.,Kim, W.K.,Kim, I.K.,Kim, J.E.,Kim, J.C.,Park, K.W. 대한소아외과학회 2007 소아외과 Vol.13 No.2

본 조사 결과는 21개 병원에서 수술 받은 환자 476명의 기록에 대한 전향적인 분석과 23명의 회원에 대한 설문 응답지에 의한 것이다. 미리 환자등록지를 배부하여 6개월 간 전향적으로 기록한 것으로 그 의의가 크다고 할 수 있다. 그러나 전향적 연구계획에 의한 것이 아니므로 통계적인 비교는 시도하지 않았고 급성충수염에 대한 회원들의 전체적인 의견과 성향을 파악하는데 중심을 두었다. 복강경수술은 아직 전체 충수절제술의 30 %에 못 미치고 있으나 설문지에 답한 회원 약 50 %가 복강경 충수절제술을 시행하고 있으며 이 숫자는 계속 증가할 것으로 보인다. 그러나 복강경수술의 결과는 아직 개복수술과 크게 차이가 없는 것으로 보인다. 한편 단순충수염에 대한 예방적 항생제투여 방법과 기간 등에 대한 설문결과에 큰 차이가 있어 이에 대한 학회 중심의 논의가 필요하다고 생각한다. A nationwide survey on acute appendicitis (AAP) was undertaken among members of the Korean Association of Pediatric Surgeons. The members were required to complete a questionnaire and the case registration form for each patient during the 6 months period from the $1^{st}$ October, 2005 to $31^{st}$ March, 2006. Questionnaires were collected from 23 members. Four hundred seventy six patients were registered from 21 hospitals where the members were working. The male to female ratio was 1.67:1. Average age was 9 years and 5 months (range 21 months-20 years). Operations were performed on average 10.4 hours (range 1-230 hours) from arrival at hospital. The average operation time was 59.1 minutes. The average admission days were 5.8 days (range 2-45 days). The most frequent symptoms were abdominal pain (95.1 %), vomiting (50.6 %) and fever (43.7%). The average duration of symptoms was 42.2 hours (range 1 hour-22 days). Leukocytosis (WBC count > 10,000) was found in 85.9%. The most popular diagnostic tools were ultrasonography and CT. Open surgery was performed in 72.1 % and laparoscopic surgery in 27.5 %. Two laparoscopic surgeries were converted to open surgery (0.4 %). Simple appendicitis was found in 54.5 % and complicated appendicitis such as abscess, gangrenous change and perforation in 45.5%. According to the questionnaires 12 hospitals were performing open surgery only in all patients. Four hospitals were performing laparoscopic surgery in all patients. Seven hospitals are performing both surgical methods according to surgeon or occasion. Regarding the use of antibiotics in acute appendicitis, three kinds of antibiotics were used in 40 % of total simple appendicitis patients. The results showed the trend of management in acute appendicitis in Korea. Especially it is necessary for the members to discuss the use of antibiotics for prophylaxis in the simple appendicitis.

괴사성 장염 - 대한소아외과학회회원 대상 전국조사 -

이석구,김대연,김신곤,김우기,김인구,김상윤,김성철,김재억,김재천,박귀원,박우현,서정민,송영택,오수명,오정탁,이남혁,이두선,이성철,전용순,정상영,정성은,최금자,최순옥,최승훈,한석주,Lee, Suk-Koo,Kim, D.Y.,Kim, S.G.,Kim, W.K.,Kim, I.K.,Kim, S.Y.,Kim, S.C.,Kim, J.E.,Kim, J.C.,Park, K 대한소아외과학회 2006 소아외과 Vol.12 No.1

본 조사 결과는 16개 병원에서 21명의 회원에 의해 수술 받은 환자 71명의 기록과 전체 설문응답지의 대상인 81명에 대한 설문 응답지를 후향적으로 분석한 것으로 자료로서의 정확성에 한계가 있으나 괴사성 장염이라는 질병에 대한 회원들의 전체적인 의견과 성향을 파악하는데 만족할 수 있었으며 회원들이 동일한 관심에 대하여 논의하는 과정 중에 각자의 관점을 정리하는 계기가 되었기를 기대한다. 앞으로 첨부한 설문지에 따라 환자마다 전향적으로 기술하여 학회에 등록하여 향후 괴사성장염에 대하여 다시 한번 논의하는 자리를 갖기를 바란다. A nationwide survey on necrotizing enterocolitis (NEC) was undertaken among members of the Korean Association of Pediatric Surgeons. The members were required to complete a questionnaire and the case registration form for each patient during the three-year period from July 2001 to June 2004. Eighty one patients were included in the questionnaire, but only 71 patients were registered from 22 members in 16 hospitals. At the same time survey on focal intestinal perforation (FIP) was undertaken and 17 patients were registered from 11 members in 10 hospitals. Total 19,041 newborns were admitted to neonatal intensive care unit during the study period. Eighty one patients (17.27 %) were underwent surgery among 469 babies who were managed under the impression of NEC. The male to female ratio was 2.1:1. The premature were 60.6 %. The most common site of involvement was ileum. Overall and operation survival of NEC were 72 % and 79 %, respectively. The survival was lower in smaller babies, multiple segments involvement and involvement of both the small and large intestine. But there was no difference in survival according to sex or prematurity. The FIP showed very similarity with NEC in terms of incidence, and the age of diagnosis and operation. But the survival was much better and 100 %. The results showed the clinical characteristics of NEC and the trend of management in NEC in Korea. In the future we hope we can discuss about this topic in prospective manner.

3個 島嶼地域 Pinus densiflora 天然生 集団의 Isoperoxidase 変異

H. G. Chung(鄭憲官),S. K. Lee(李錫求),K. J. Lee(李景俊) 한국육종학회 1982 한국육종학회지 Vol.14 No.1

The objective of this study was to identify and compare the genetic variations in natural populations of Pinus densiflora in three islands in Korea. Using starch gel electrophoresis, number and frequency of isoperoxidase bands in mature needle leaves were observed in three subpopulations each from three islands, Jeju, Hongdo, and Ulreung. A total of 11 different bands were observed. Among the three islands, Jeju populations showed highest number of bands(7.8 bands per sample) in average, and 6.2 and 6.1 bands per sample were obtained from Ulreung and Hongdo, respectively. The bands, G(Rf 0,3) and Y (Rf 0.65) were observed in all the samples with no variation. The S band showed the largest variation in frequency which ranged from 10 to 66 %. Jeju populations were considered to be the best natural stands among the populations studied and are recommended to be preserved for gene conservation, judged by their good growth, straight stem, and large genetic variation.

구연 / 제 3 회의장 / 간 26 : 간이식을 받은 B 형 간염 환자에서 lamivudine / B 형 간염 면역글로불린 단기 병합투여군과 면역글로불린 장기 단독투여군 간의 B 형 간염 재발을 비교

박상종(S . W . Park),백승운(S . W . Paik),최문석(M . S . Choi),이준혁(J . H . Lee),이풍렬(P . L . Rhee),고광철(K . C . Kho),이종철(J . C . Rhee),최규완(K . W . Choi),김성주(S . J .Kim),조재원(J . W . Joh),이석구(S . K . Lee) 대한소화기학회 1999 대한소화기학회 추계학술대회 Vol.1999 No.-

1999년도 한국 신생아외과 현황 -대한소아외과학회 회원대상 전국조사(제 2 차)-

김우기,김상윤,김신곤,김인구,김재천,김현학,박귀원,박우현,송영택,오수명,유수영,이두선,이명덕,이성철,이석구,서정민,정상영,정성은,정을삼,정풍만,Kim, W.K.,Kim, S.Y.,Kim, S.K.,Kim, I.K.,Kim, J.C.,Kim, H.H.,Park, K.W.,Park, W.H.,Song, Y.T.,Oh, S.M.,Yoo, Y.S.,Lee, D.S 대한소아외과학회 2001 소아외과 Vol.7 No.1

To understand the current status of neonatal surgery in Korea, a survey was made among the 36 members of the Korean Association of Pediatric Surgeons. The response rate was 75 % (26 surgeons in 20 hospitals). Five hundred fifty three neonatal surgical patients treated in 1999 were analyzed. Regional numbers of patients were closely related to the regional population in most areas. Ano-rectal malformations (17 %), pyloric stenosis (16 %), Hirschsprung's disease (13 %), atresia/stenosis of the gut (11 %), esophageal atresia (8 %) were the most common anomalies treated. The majority of operations were done within the first week of life. Seventy one per cent of cases were major life threatening or so-called neonatal index cases. Over-all mortality was 8 per cent. Higher mortality was observed in patients with diaphragmatic hernia (26 %), gastro-intestinal perforation (18 %), NEC (18 %), and esophageal atresia(14 %). Higher mortality was observed in patients with extremely low birth weight (33%) and low birth weight (18 %). Associated anomalies were observed in 20 %. Prenatal ultrasound was performed in 36 per cent with sensitivity of 20 %. Result of this study was compared to the previous report (1994) and that of Japan (1998).

人爲倍數性 林木에 關한 硏究 XII 人爲 4倍體 아까시나무 次代의 減數分裂

C. S. Kim(金鼎錫),S. K. Lee(李錫求),김승진 한국육종학회 1974 한국육종학회지 Vol.6 No.1

The chromosome configuration and its behavior in the meiosis of pollen mother cell were observed on four individuals of Robinia pseudoacacia; one was colchitetraploid and the others were one of triploid and two of tetraploid selected from the progenies of colchitetraploid. 1. Chromosome configuration for colchitetraploid, mother tree, was appeared to be slightly irregular and however, most of nuclear plates showed not only chromosome pairing of 20 Ⅱ at metaphaseⅠbut chromosome distribution of 20 at metaphase Ⅱ. 2. At metaphaseⅠ, the numbers of nuclear plates with early separation chromosome were observed with the lowest proportion in the progeny-3 of triploid and with the highest proportion in progeny-5 of tetraploid with the appearance rate of 39.87% 3. At metaphase Ⅱ, the numbers of nuclear plates with early separation chromosome were observed with the lowest proportion in the progeny-16 of tetraploid and with the highest proportion in the progeny -3 of triploid with the appearance of 8.33%. The appearance rate of chromosome bridge between chromosome groups was minimum in the progeny-16 of tetraploid. 4. At anaphaseⅠ, the numbers of nuclear plates with lagging chromosome were observed with the lowest proportion in the progeny-5 of tetraploid and with the highest proportion in the progeny -3 of triploid with the appearance of 27.87%. The maximum appearance rate of chromosome bridge was also in the progeny-3 of the triploid. 5. At anaphase Ⅱ, the number of nuclear plates with lagging chromosome were observed with the lowest proportion in the colchitetraploid, mother tree and with the highest proportion in the progeny-3 of triploid with the appearance rate of 33.45%. The appearance rate of chromosome bridge was maximum in the progeny-3 of triploid. 6. The number of normal pollen sporad was maximum in the colchitetraploid, mother tree.

자작나무 성숙목의 근주맹아를 이용한 기내증식

문흥규(H . K . Moon),윤양(Y . Youn),현영일(Y . I . Hyun),이석구(S . K . Lee) 한국산림과학회 1991 한국산림과학회지 Vol.80 No.4

Effective micropropagation was achieved by axillary bud culture from stool shoots of 15-year-old Betula platyphylla var. japonica. Shoot development and proliferation from the explants were successful on WPM supplemented with 0.5 or 1.0㎎/ℓ BAP. All the regenerated shoots rooted when transfered to GD medium containing 0.2㎎/ℓ IBA. After transplaning to soil more than 95% of the plantlets survived and showed normal growth. The results demonstrate that masspropagation of selected mature trees is feasible using tissue culture technique.

RFLP 기법을 이용한 포플러 엽록체 DNA 의 분석

이재순(J . S . Lee),노은운(E . W . Noh),이석구(S . K . Lee),권기원(K . W . Kwon) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.1

In woody species with a long life span, the studies on inheritance of any trait may be very time consuming and laborious. Chloroplast DNA(cpDNA) has been a valuable tool in such studies since it has several unique features such as limited genome size and cytoplasmic inheritance. In the present study, cpDNAs from five different species of Populus (P. alba, P. glandulosa, P. alba x P. glandulosa, P. davidiana, and P. nigra), and Nicotiana tabacuna were compared with regard to restriction fragment length polymophism. The results showed that cpDNAs among the species were very conserved, although some polymorphisms were observed when the DNAs were digested with restriction enzyme EcoRI or KphI. The other enzymes (Bgl II, and PstI) tested produced identical restriction fragmentation pattern among the species. However, cpDNAs from all the five Populus species showed different restriction fragmentation pattern from that of tobacco with the four restriction enzymes tested. Southern hybridization with tobacco rbcL gene fragment as a probe also produced identical pattern among Populus species. The results indicate that cpDNAs in the genus are very well conserved during evolution.

한국의 항문-직장 기형 -1999년도 대한소아외과학회 회원 대상 전국 조사-

이명덕,김상윤,김우기,김인구,김재억,김재천,김현학,박귀원,박우현,송영택,오수명,유수영,이두선,이석구,이성철,장수일,정상영,정성은,정을삼,정풍만,Lee, M.D.,Kim, S.Y.,Kim, W.K.,Kim, I.K.,Kim, J.U.,Kim, J.C.,Kim, H.H.,Park, K.W.,Park, W.H.,Song, Y.T.,Oh, S.M.,Yoo, S.Y 대한소아외과학회 2000 소아외과 Vol.6 No.2

The purpose of the survey was to clarify the clinical features and management of the anorectal malformations (ARM) in Korea. Twenty-seven members of the Korean Association of Pediatric Surgeons were surveyed. The members completed a case registration form for each patient during the two years period of 1996-1997, and a questionnaire that contained their management preferences for primary and complicated patients. Twenty-four members of the 22 institutions registered 295 cases of the ARM, and 27 out of 34 members (77.1 %) responded to the questionnaire. The patients were classified by the Wingspread classification of ARM(1984). The case registration form was a modification of Wingspread workshop for the postoperative assessment and case registration. The male to female ratio was 1.7 : 1. The average number of patients per surgeon was 6.1 cases per year. Prematurity(< 36 weeks) was present in 1.9 % of cases and low birth weight (< 2.6 Kg) in 12.1 %. Among 187 male patients, 62 were high(H) type, 29 intermediate(l) type, 88 low(L), and 8 unclassified. In female, there were 2 cases of cloacal anomalies(C), 4 H type, 30 I type, 66 L type and 6 unclass ified. For male, 87.9 % of H and I type were operated by the Pena procedure, but only 7.9 % in L types. In female, all of C, H and I types, and 40.9 % of L type were done by the Pena procedure. One case expired (MR; 0.003%) as a result of surgical complication. Over-all complication rate was 12.5 %. Among 6 cases of reoperation, 4 were failed Pena procedures. Among 140 colostomies sigmoid colon was utilized in 75.7%, and loop colostomy was commonly used. Ten surgeons prefered primary maturation of the stoma. In conclusion, posterior sagittal anorectoplasty is popular for high types of ARM in this country, but considerable number of patients are still suffering from failed operations. For better understanding and analysis, necessity of prospective study by new classification was discussed.

소아외과의 지수 질환 - 대한소아외과학회 정회원을 대상으로 한 2000년도 전국 조사 -

이명덕,김상윤,김우기,김인구,김성철,김신곤,김재억,김재천,김현학,박귀원,박우현,서정민,송영택,오수명,유수영,이두선,이석구,이성철,정상영,정성은,정을삼,정풍만,조마해,Lee, M.D.,Kim, S.Y.,Kim, W.K.,Kim, I.K.,Kim, S.C.,Kim, S.K.,Kim, J.E.,Kim, J.C.,Kim, H.H.,Park, K.W.,Park, W 대한소아외과학회 2001 소아외과 Vol.7 No.2

Membership qualifications and recognition of the subspecialty training programs by the Korean Association of Pediatric Surgeons(KAPS) have been determined by criteria based on the number of neonatal and total pediatric surgical cases registered, since 1989 (Index cases). The numbers are based on a survey of the 14 founding members of the society by Jung et al. in 1987. The current survey is to review the present status of the index cases from 1997 to 1999 among 36 members of the KAPS. Two surveys were undertaken for this study. In the first survey, cases were collected by the registration form, composed of neonatal(N), important pediatric surgical cases (I), tumor and other similar operations(T) and other common pediatric surgical cases(O). Thirty members responded. and the result was discussed at the Topic Discussion section of the 15th Annual Congress of KAPS, 2000. The second survey analyzed additive data, proposed during discussion at the Congress. Twenty-three members responded. This report is the analysis of the both surveys. The average numbers of the cases/year/surgeon of N, I, T, O and total were 19.5, 51.8, 9.5, 77.1 and 356.5. respectively. The number of index cases(N+I+T)/year/surgeon was 80.8. The ratio of (N+I+T)/total cases was 0.3. Seventeen of 30 members have more than 20 cases of N per year. Twelve members have more than 150 cases, and 13 do more than 100 cases of I per year. Fourteen members have more than 10 cases of T per year. Nineteen and 10 members experienced more than 150 and 100 of index cases(N+I+T) per year. A new list of the index cases and coding system are proposed for the future regular update. 대한소아외과학회의 연례 행사인 2000년도 주제토의를 소아외과의 지수 질환으로 정하여 1999년 말 현재 3년 이상의 진료 실적을 가진 대한소아외과학회 정회원 36명을 대상으로 미리 작성된 조사서에 의하여 2차례의 수술 증례 수 등록을 시행하였다. 대상 환자는 1997년부터 1999년 말까지 3년간 수술이 시행된 환자로 하였으며, 1차 조사에서 30명이 응답하였고, 미비점 보완을 위한 2차 조사에는 26명이 응답하여 이 성적을 요약하였다. 본 성적을 바탕으로 향 후 대한소아외과학회의 지수 질환 대상 목록표를 작성 제시하였으며, 이를 코드화 하고자 제안하였다.