난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium

Lee, E.H.,Moon, K.E.,Kim, T.G.,Lee, S.D.,Cho, K.S. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.120 No.6

Kinetic and enzymatic inhibition experiments were performed to investigate the effects of methanethiol (MT) and hydrogen sulfide (H<SUB>2</SUB>S) on methane oxidation by a methane-oxidizing consortium. In the coexistence of MT and H<SUB>2</SUB>S, the oxidation of methane was delayed until MT and H<SUB>2</SUB>S were completely degraded. MT and H<SUB>2</SUB>S could be degraded, both with and without methane. The kinetic analysis revealed that the methane-oxidizing consortium showed a maximum methane oxidation rate (V<SUB>max</SUB>) of 3.7 mmol g-dry cell weight (DCW)<SUP>-1</SUP> h<SUP>-1</SUP> and a saturation constant (K<SUB>m</SUB>) of 184.1 μM. MT and H<SUB>2</SUB>S show competitive inhibition on methane oxidation, with inhibition values (K<SUB>i</SUB>) of 1504.8 and 359.8 μM, respectively. MT was primary removed by particulate methane monooxygenases (pMMO) of the consortium, while H<SUB>2</SUB>S was degraded by the other microorganisms or enzymes in the consortium. DNA and mRNA transcript levels of the pmoA gene expressions were decreased to ~10<SUP>6</SUP> and 10<SUP>3</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after MT and H<SUB>2</SUB>S degradation, respectively; however, both the amount of the DNA and mRNA transcript recovered their initial levels of ~10<SUP>7</SUP> and 10<SUP>5</SUP>pmoA gene copy number g-DCW<SUP>-1</SUP> after methane oxidation, respectively. The gene expression results indicate that the pmoA gene could be rapidly reproducible after methane oxidation. This study provides comprehensive information of kinetic interactions between methane and sulfur compounds.

H-beam 압연 최종패스에서 소재 후단부의 상·하 압하력 변화에 대한 연구

김성기(S. G. Kim),정현석(H. S. Jung),위창현(C. H. Wee),문홍길(H.G. Moon),서경호(K. H. Seo),변상민(S. M. Byon),이영석(Y. S. Lee) 한국소성·가공학회 2023 한국소성가공학회 학술대회 논문집 Vol.2023 No.5

Purification and characterization of a novel fibrinolytic α chymotrypsin like serine metalloprotease from the edible mushroom, Lyophyllum shimeji

Moon, S.M.,Kim, J.S.,Kim, H.J.,Choi, M.S.,Park, B.R.,Kim, S.G.,Ahn, H.,Chun, H.S.,Shin, Y.K.,Kim, J.J.,Kim, D.K.,Lee, S.Y.,Seo, Y.W.,Kim, Y.H.,Kim, C.S. Society for Bioscience and Bioengineering, Japan ; 2014 Journal of bioscience and bioengineering Vol.117 No.5

A novel fibrinolytic enzyme was purified from Lyophyllum shimeji, a popular edible mushroom in Asia. The enzyme was purified using combination of anion exchange chromatography on a Mono Q 5/5 column and size exclusion gel filtration chromatography on Superdex 200 100/300 column. This purification protocol resulted 80.9-fold purification of the enzyme and a final yield of 5.7%. The molecular weight of the purified enzyme was estimated to be 21 kDa by SDS-PAGE and size exclusion gel filtration. The N-terminal amino acid sequence was found to be ITFQSASP, which is dissimilar from that of known fibrinolytic enzymes. The purified enzyme was a neutral protease with an optimal reaction pH and temperature of 8.0 and 37<SUP>o</SUP>C, respectively. Enzymatic activity was inhibited by Cu<SUP>2+</SUP> and Co<SUP>2+</SUP>. It was also significantly inhibited by PMSF and TPCK. Furthermore, it was found to exhibit a higher specificity for S-7388, a well-known chymotrypsin chromogenic substrate, indicating chymotrypsin like serine metalloprotease. The relative fibrinolytic activity of 5 μg purified enzyme have two fold more activity than 1 unit/ml of plasmin on fibrin plate. Furthermore, purified enzyme preferentially hydrolyzed the Aα-chain followed by the Bβ- and γ-chain of fibrinogen, which is precursor of fibrin. Therefore, these data suggests that the fibrinolytic enzyme derived from edible mushroom, L. shimeji, might be useful for thrombolytic therapy and preventing thrombotic disease.

H형강 롤포스 예측 모델 개발

정현석(H. S. Jung),위창현(C. H. Wee),문홍길(H. K. Moon),서경호(K. H. Seo),김태형(T. H. Kim),배병철(B. C. Bae),이영석(Y. S. Lee) 한국소성·가공학회 2023 한국소성가공학회 학술대회 논문집 Vol.2023 No.11

H대학교 아음속 풍동 개념설계

장조원,전창수,김문상,이열,문희장,송병흠,김학봉,Chang, J.W.,Jeon, C.S.,Kim, M.S.,Lee, Y.,Moon, H.J.,Song, B.H.,Kim, H.B. 한국항공운항학회 2005 한국항공운항학회지 Vol.13 No.4

A closed-circuit type wind tunnel is designed, which has a test section with the dimensions $1.2(W){\times}1.2(H){\times}3.4(L)$. A subsonic wind tunnel is designed to improves educational circumstances and promote ground tests. It is constituted of an exchangeable test section, first and second diffusers, a fan, a settling chamber, a contraction, and 4 corners. The maximum velocity in the test section is 70m/s and the contraction ratio is 6.25:1. Input power in the wind tunnel is about 96.1 kw (128.8 hp) and its energy ratio is 3.89. It has the dimension of about $7.4(W){\times}3.6(H){\times}21.7m(L)$. The wind tunnel designed in this investigation will be an effective educational and investigational equipment.

Micropropagation of Superior Variety of Japanese Pepper Tree (Zanthoxylum piperitum Dc.)

Son, S. H 한국식물조직배양학회 1995 식물생명공학회지 Vol.22 No.3

Myo-inositol이 돼지 난모세포의 체외성숙에 미치는 영향

조인식,한효원,이상미,박효영,정영희,문승주,강승률,강만종 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

본 연구는 미성숙 돼지 난모세포의 체외 성숙에 있어서 myo-inositol의 영향을 알아보기 위하여 실시하였다. 미성숙 돼지 난모세포을 myo-inositol을 포함하는 또는 포함하지 않은 체외 성숙배지에서 44시간 체외 성숙을 유도하였을 때 성숙율은 myo-inositol을 첨가한 성숙배지에서 성숙시킨 실험구에 유의하게 높았다(P<0.05). Myo-inositol에 의한 성숙율 향상에 있어서 난구세포의 영향을 알아보기 위하여 난추세포의 치밀도에 따라 분류하여 미성숙 난모세포을 myo-inositol을 포함하는 체외 성숙배지에서 배양하였을 때 난구세포가 치밀한 미성숙 난모세포에서가 더 높은 성숙율을 나타내었다(P<0.05). 그러나 난구세포가 치밀하지 않은 미성숙 난모세포도 myo-inositol을 포함하는 성숙배지에서 배양하면 성숙율은 대조구에 비하여 유의하게 높았다(P<0.05). 이러한 결과는 돼지 난모세포의 체외 성숙배지에 myo-inositol의 첨가는 체외 성숙율을 향상시킬 수 있음을 나타내고 있다. This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing lO1U/ml PMSG, 10 IU/ml HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase II oocytes 44h after culture was higher in the myo-inositol group(P<O.05). To study effects of cumulus cell on the maturation induced by myo-inositol, we examined the maturation status of cumulus-enclosed or cumulus-denuded porcine follicular oocytes. The rates of maturation were significantly higher in the cumulus-enclosed oocytes(P<O.05). However, the maturation rates of cumulus-denuded oocytes cultured in medium containing myo-inositol were higher than those of control group(P<O.05). Our results suggest that myo-inositol may affect meiotic progression of porcine follicular oocytes and supplementation of myo-inositol in maturation medium may be useful for the in vitro maturation of porcine follicular oocytes.

Kinetics determination of electrogenerated hydrogen peroxide (H₂O₂) using carbon fiber microelectrode in electroenzymatic degradation of phenolic compounds

Cho, S.H.,Jang, A.,Bishop, P.L.,Moon, S.H. Elsevier Scientific Pub. Co 2010 Journal of hazardous materials Vol.175 No.1

The kinetics of electrogenerated hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>), which can activate peroxidases in an electroenzymatic process, was examined by an amperometric technique using a carbon fiber microelectrode that was modified by polyaniline (PAn) film and platinum particles. The electrogeneration of H<SUB>2</SUB>O<SUB>2</SUB> was found to be dependent on the pH and applied potential, and resulting in a variable current response of the carbon fiber microelectrode. The highest amount of H<SUB>2</SUB>O<SUB>2</SUB> was electrogenerated when 2.3V was applied between the Pt/Ti anode and a reticulated vitreous carbon (RVC) cathode at pH 6.0, with a current response of 0.0190μAmin<SUP>-1</SUP>. Phenol was completely degraded by the electroenzymatic reaction of the immobilized horseradish peroxidase (HRP), and the time required for the electrogeneration of H<SUB>2</SUB>O<SUB>2</SUB> increased according to the initial concentration of phenol. The degradation stoichiometric ratio between the electrogenerated H<SUB>2</SUB>O<SUB>2</SUB> and the aqueous phenol under HRP immobilized on RVC was found to be 1:1.

구개인두성형술 후 음성의 음향학적 변화

홍기환,김성완,윤희완,조윤성,문승현,이상헌 한국음성과학회 2001 음성과학 Vol.8 No.2

The primary sound produced by the vibration of vocal folds reaches the velopharyngeal isthmus and i s directed both nasally and orally. The proportions of the each component i s determined by the anatomical and functional status of the soft palate. The oral sounds composed of oral vowels and consonants according to the status of vocal tract, tongue, palate and lips. The nasal sounds composed of nasal consonants and nasal vowels, and further modified according to the status of the nasal airway, so anatomical abnormalities in the nasal cavity will influence nasal sound. The measurement of nasal sounds of speech has relied on the subjective scoring by listeners. The nasal sounds are described with nasality and nasalization. Generally, nasality has been assessed perceptually in the effect of ma illofacial procedures for Cleft palate, sleep apnea, snoring and nasal disorders. The nasalization is considered as an acoustic phenomenon. Snoring and sleep apnea i s a typical disorders due to abundant velopharyn . The sleep apnea has been known as a cessation of breathing for at least 10 seconds during sleep. Several medical and surgical methods for treating sleep apnea have been attempted. The uvulopalatopharynoplasty(UPPP) involves removal of 1.0 to 3.0 cm of soft palate tissue with removal of redundant orophatyngeal. mucosa and lateral tissue from the anterior and sometimes posterior faucial pillars. This procedure results in a shortened soft palate and a possible risk following this surgery may be velopharyngeal malfunctioning due to the shortened palate. Few researchers have systematically studied the effects of this surgery as it relates to. speech production. Some changes in the voice quality such as resonance (nasality), articulation, and phonation have been reported. In view of the conflicting reports discussed, there remains some uncertainty about the speech status in patients following the snoring and sleep apnea surgery. The study was conducted in two phases: 1) acoustic analysis of oral and nasal sounds, and 2) evaluation of nasality.