A new tyrosinase biosensor based on covalent immobilizationof enzyme on N-(3-aminopropyl) pyrrole polymer film

Rajesh,K.Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polymer film of N-amino substituted pyrrole monomer has been prepared for covalent immobilization of enzyme for biosensing applications, illustrated by tyrosinase (PPO).The tyrosinase enzyme retains its bioactivity when being immobilized on N-substituted pyrrole polymer film by covalent bonding.The enzyme electrode was characterized by UV.Vis and infrared spectroscopy.Phenolic compounds were quantitatively estimated by the direct electrochemical reduction of enzymatically liberated quinone species at 0.2 V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode show sensitivity of 57.6, 71.4 and 45.8 mAM1cm2 and a linear response range of 1.8.170.2, 1.3.110.1 and 2.1.168 lM for phenol, catechol and p-cresol, respectively. The biosensor exhibits a lowest detection limit of 0.9, 0.7 and 1.1 lM, for phenol, catechol and p-cresol, respectively and a period of stable sensitivity of 3 months at 4.5 C.

Simultaneous co-immobilization of enzyme and a redox mediatorin polypyrrole film for the fabrication of an amperometricphenol biosensor

Rajesh,S.S. Pandey,W. Takashima,K. Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polypyrrole lm has been prepared on indium tin oxide coated glass plate by electropolymerization of pyrrole in anaqueous solution of enzyme, tyrosinase (PPO), and a redox mediator potassium hexacyanoferrate (II). The enzyme retains its bio-activity well within the polymer lm. Phenol concentration in aqueous solution was quantitatively estimated by monitoring the elec-tron exchange occurring in redox mediator within the polymer matrix i.e. direct reduction of Fe (III) to Fe (II) ion at +0.12V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode (PPO/Fe2+/PPY/ITO) show a sensitivityof 0.33 AM. 1 cm. 2 and a linear response range of 4.5107.4l M for phenol in aqueous medium. The electrode exhibits a responsetime of about 80 second and is stable for 4 months at 4.C..

Effect of Preoperative Vitamin D Levels on Functional Performance after Total Knee Arthroplasty

Rajesh Navin Maniar,Aniket Machindra Patil,Adit Rajesh Maniar,Bharat Gangaraju,Jaivardhan Singh 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.2

Background: Low vitamin D levels affects muscle function. Vitamin D and calcium deficiency cause osteomalacic myopathy and poor functional recovery after hip fractures. The relationship of vitamin D and functional performance after total knee arthroplasty (TKA) is not previously reported. Methods: Influence of vitamin D on functional performance before and after TKA was reviewed retrospectively in 120 patients. Of these, 64 had vitamin D deficiency (25-hydroxy vitamin D < 30 ng/mL) preoperatively. All 120 patients received vitamin D oral supplementation postoperatively. Functional parameters including Western Ontario and McMaster Osteoarthritis Index (WOMAC), Short-Form Health Survey questionnaire, and Knee Society Score were assessed pre- and postoperatively at 3 months. Results: Preoperative function was significantly lower in osteoarthritic patients with vitamin D deficiency (WOMAC score; p = 0.040), but at 3 months all functional scores were similar. Conclusions: We concluded that vitamin D deficiency has a negative effect on function in knee osteoarthritic patients. However, postoperative vitamin D supplementation can lead to functional recovery in these patients. Thus, TKA should not be delayed in vitamin D deficient patients; rather supplementation in the postoperative period is preferable to achieve comparable functional outcome at 3 months to patients with vitamin D sufficiency.

A novel hybrid technique to treat flush mesenteric arterial occlusion in acute-on-chronic mesenteric ischemia

Rajesh Girdhardas Mundhada,Atul Dharmaraj Rewatkar,Aishwarya Atul Rewatkar,Anju Rajesh Mundhada,Nikita Navin Chandak 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

Acute mesenteric occlusion is a life-threatening condition with a significant mortality rate and requires prompt diagnosis and revascularization using open repair, a hybrid procedure, or endovascular intervention. The open approach is associated with significant morbidity and mortality, while endovascular procedures are done by the antegrade or retrograde open mesenteric stent technique and have been observed to improve the prognosis and outcomes of mesenteric ischemia. We report three cases of acute-on-chronic mesenteric ischemia secondary to occlusion with failed percutaneous mesenteric artery stenting. They were successfully treated using a novel hybrid technique, without the need for mesenteric arteriotomy or subsequent arterial repair.

Ratio-Cum-Product Estimators of Population Mean Using Known Population Parameters of Auxiliary Variates

Tailor, Rajesh,Parmar, Rajesh,Kim, Jong-Min,Tailor, Ritesh The Korean Statistical Society 2011 Communications for statistical applications and me Vol.18 No.2

This paper suggests two ratio-cum-product estimators of finite population mean using known coefficient of variation and co-efficient of kurtosis of auxiliary characters. The bias and mean squared error of the proposed estimators with large sample approximation are derived. It has been shown that the estimators suggested by Upadhyaya and Singh (1999) are particular case of the suggested estimators. Almost ratio-cum product estimators of suggested estimators have also been obtained using Jackknife technique given by Quenouille (1956). An empirical study is also carried out to demonstrate the performance of the suggested estimators.

Heat Flux Measurements in High Velocity Oxygen-Fuel Torch Flow for Testing High Thermal Materials

Rajesh Kumar Chinnaraj,Seong Man Choi(최성만),Seong Min Hong(홍성민) 한국추진공학회 2021 한국추진공학회지 Vol.25 No.2

A commercial HVOF torch (originally designed for coating applications) has been modified as a high temperature flow source for material testing. In this study, a water cooled commercial Gardon gauge was used to measure heat fluxes at four locations away from the nozzle exit. The cooling water temperature data were used to calculate calorimetric heat fluxes at the same locations. The heat fluxes from both methods were compared and the calorimetric heat fluxes were found to be many times higher than the Gardon gauge heat fluxes. A hypothesis is applied to the calorimetric method to understand the discrepancy seen between the methods. The Gardon gauge heat fluxes are seen to be in the range of the hypothesized calorimetric calculations. This can be considered as a considerable validation for the hypothesis, but further refinement needed using appropriate numerical models.

Proteomic Analysis of Bovine Longissimus Muscle Satellite Cells during Adipogenic Differentiation

Rajesh, Ramanna Valmiki,Park, Mi-Rim,Heo, Kang-Nyeong,Yoon, Du-Hak,Kim, Tae-Hun,Lee, Hyun-Jeong Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.5

Satellite cells are skeletal muscle progenitor/stem cells that reside between the basal lamina and plasma membranes of skeletal fibers in vivo. These cells can give rise to both myogenic and adipogenic cells. Given the possible role for differentiation of satellite cells into adipocytes in marbling and in some pathological disorders like sarcopenia, knowledge of the proteins involved in such process remains obscure. Using two-dimensional polyacrylamide gel electrophoresis coupled with mass spectrometry, we investigated the proteins that are differentially expressed during adipogenic differentiation of satellite cells from bovine longissimus muscle. Our proteome mapping strategy to identify the differentially expressed intracellular proteins during adipogenic differentiation revealed a total of 25 different proteins. The proteins up-regulated during adipogenic differentiation of satellite cells like Cathepsin H precursor, Retinal dehydrogenase 1, Enoyl-CoA hydratase, Ubiquinol-cytochrome-c reductase, T-complex protein 1 subunit beta and ATP synthase D chain were found to be associated with lipid metabolism. The down-regulated proteins like LIM protein, annexin proteins, cofilin-1, Rho GDP-dissociation inhibitor 1 and septin-2, identified in the present study were found to be associated with myogenesis. These results clearly demonstrate that the adipogenic conversion of muscle satellite cells is associated with the up-regulated and down-regulated proteins involved in adipogenesis and myogenesis respectively.

Facile synthesis of TiO2/ZnFe2O4 nanocomposite by sol-gel auto combustion method for superior visible light photocatalytic efficiency

Rajesh Jagannath Tayade,Kalithasan Natarajan,Puspendra Singh,Hari Chand Bajaj 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.6

Photocatalytic composite materials having photon absorption capability in the range of visible light were synthesized by loading TiO2 (5, 10, 15, and 20 wt%) on ferrite nanocomposites by sol-gel auto-combustion method. The synthesized nanocomposites were analyzed using X-ray diffraction, Transmission electron microscopy, diffuse reflectance spectroscopy and N2 adsorption techniques. The generation of photo active hydroxyl radicals for all the synthesized composites was found higher under the irradiation of red LED (RLED irradiation) which was confirmed by degradation of rhodamine B dye under irradiation of RLED. Photocatalytic activity of the synthesized nanocomposites was also carried out under irradiation of ultraviolet (UVLED) and blue (BLED) light emitting diodes, which is comparatively less than for the reaction under red LED irradiation. The operational parameters like catalyst amount, pH and concentration of dye solution were studied and ESI-MS degradation pathway is proposed by analyzing the degraded samples.

Pigeonpea 의 Fusarium 시들음병에 대한 화학적 방제

Rajesh Singh 한국균학회 1998 韓國菌學會誌 Vol.26 No.4

N/A

Cup-Cage Construct Using Porous Cup with Burch-Schneider Cage in the Management of Complex Acetabular Fractures

( Rajesh Malhotra ),( Deepak Gautam ) 대한고관절학회 2019 Hip and Pelvis Vol.31 No.2

Purpose: Cup-cage construct technique was developed to address the massive acetabular defects during revision hip arthroplasty. Indications have extended to complex acetabular fractures with pelvic discontinuity necessitating acute total hip arthroplasty. However, its use is constrained in low socioeconomic countries due to non-availability of the original cages from Trabecular Metal Acetabular Revision System and high cost. We used a novel technique using the less expensive Burch-Schneider (BS) cage and Trabecular Metal Revision Shell (TMRS) to address the problem. Materials and Methods: We reviewed a consecutive series of 8 cases of acetabular fractures reconstructed using a ‘cup-cage construct’ technique using a BS cage along with a TMRS. The mean age of the patients was 61.4 years. Patients were followed up for a mean period of 50.5 months (24 to 72 months). The patients were assessed clinically with Harris Hip Score and radiologically with serial X-rays. Results: All the patients were available at the latest follow up. The mean Harris Hip Score was 87.2. There was no radiological evidence of failure. One patient had dislocation two months following the surgery, which was treated by closed reduction and hip abduction brace. One patient developed an infection at 3 weeks necessitating debridement. The same patient had sciatic nerve palsy that recovered after 4 months. Conclusion: This novel technique of the cup-cage construct seems to provide a stable construct at short to midterm follow-up. However, a long-term follow up would be required.