Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

Mode III fracture analysis of piezoelectric materials by Trefftz BEM

Qing-Hua Qin 국제구조공학회 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.2

Mode III fracture analysis of piezoelectric materials by Trefftz BEM

Qin, Qing-Hua Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.2

Applications of the Trefftz boundary element method (BEM) to anti-plane electroelastic problems are presented in this paper. Both direct and indirect methods with domain decomposition are discussed in details. Each crack is treated as semi-infinite thin slit defined in a subregion, for which a particular solution of the anti-plane problem, satisfying exactly the crack-face condition, is derived. The stress intensity factors defined at each crack tip can be directly computed from the coefficients of the particular solution. The performance of the proposed formulation is assessed by two examples and comparison is made with results obtained by other approaches. The Trefftz boundary element approach is demonstrated to be suitable for the analysis of the anti-plane problem of piezoelectric materials.

Low Loading of Rh<i>_x</i> P and RuP on N, P Codoped Carbon as Two Trifunctional Electrocatalysts for the Oxygen and Hydrogen Electrode Reactions

Qin, Qing,Jang, Haeseong,Chen, Lulu,Nam, Gyutae,Liu, Xien,Cho, Jaephil Wiley (John WileySons) 2018 ADVANCED ENERGY MATERIALS Vol.8 No.29

Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China from 2012 to 2013

Han-Qin Shen,Zhuan-Qiang Yan,Fan-Gui Zeng,Chang-Tao Liao,Qing-Feng Zhou,Jian-Ping Qin,Qingmei Xie,Yingzuo Bi,Feng Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) wereisolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes ofthese strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, whilethe other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineageh9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at thesame position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200–202, and had an additional oneat residues 295–297 in HA1. Notably, 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that theviruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of newH9N2 strains to better prepare for the next epidemic or pandemic outbreak of H9N2 AIV infections in chicken flocks.

Genetic Effects of Polymorphisms in Myogenic Regulatory Factors on Chicken Muscle Fiber Traits

Yang, Zhi-Qin,Qing, Ying,Zhu, Qing,Zhao, Xiao-Ling,Wang, Yan,Li, Di-Yan,Liu, Yi-Ping,Yin, Hua-Dong Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6

The myogenic regulatory factors is a family of transcription factors that play a key role in the development of skeletal muscle fibers, which are the main factors to affect the meat taste and texture. In the present study, we performed candidate gene analysis to identify single-nucleotide polymorphisms in the MyoD, Myf5, MyoG, and Mrf4 genes using polymerase chain reaction-single strand conformation polymorphism in 360 Erlang Mountain Chickens from three different housing systems (cage, pen, and free-range). The general linear model procedure was used to estimate the statistical significance of association between combined genotypes and muscle fiber traits of chickens. Two polymorphisms (g.39928301T>G and g.11579368C>T) were detected in the Mrf4 and MyoD gene, respectively. The diameters of thigh and pectoralis muscle fibers were higher in birds with the combined genotypes of GG-TT and TTCT (p<0.05). Moreover, the interaction between housing system and combined genotypes has no significant effect on the traits of muscle fiber (p>0.05). Our findings suggest that the combined genotypes of TT-CT and GG-TT might be advantageous for muscle fiber traits, and could be the potential genetic markers for breeding program in Erlang Mountain Chickens.

Degradation Model and Evaluation Criteria for the Seismic Behavior of Corrosion H-section Steel Column

Guang-chong Qin,Tao Lan,Qing Qin,Shan-hua Xu,Zong-xing Zhang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.8

The cross section would be reduced and the material properties of the steel structure would be deteriorated under the corrosion environment, which will have different effects on the earthquake resistant behavior. This paper takes corroded H-steel column as the research object. Firstly, the influence on the failure mode, bearing capacity, hysteretic properties, ductility and hysteretic energy is analyzed from specific factors which consist of different width-to-thickness on flange, different height-to-thickness on web and different axial compression ratios by FEA. The results show that, the area of the hysteresis loop diminishes gradually, the bearing capacity decrease clearly, the accumulative energy dissipation and ductility coefficient decreased continuously, and the degradation laws of buckling load, peak load, hysteretic energy and ductility coefficient of corroded H-steel columns are thereby established. When the thickness of flange decreased by 30%, the peak load decreased by 32% and hysteretic energy decreased by 77%. However, the peak load decreased by 14% and hysteretic energy decreased by 40% when the thickness of web decreased by 30%. When the axial compression ratio is 0.6, and the mass loss ratio of corroded H-steel columns reached 22%, the peak load and hysteretic energy decreased by 28% and 51% respectively. Secondly, the hysteretic model of load-displacement of corroded H-steel members under cyclic loading is proposed based on the simulation results and the degradation model of characteristic point of skeleton line was given. Finally, the evaluation criteria are proposed based on the seismic performance objectives, and the classification of failure types as well as the overall performance evaluation of corroded steel members are carried out.

The Association between Educational Attainment and the Risk of Nonalcoholic Fatty Liver Disease among Chinese Adults: Findings from the REACTION Study

( Yuanyue Zhu ),( Long Wang ),( Lin Lin ),( Yanan Huo ),( Qin Wan ),( Yingfen Qin ),( Ruying Hu ),( Lixin Shi ),( Qing Su ),( Xuefeng Yu ),( Li Yan ),( Guijun Qin ),( Xulei Tang ),( Gang Chen ),( Shua 대한소화기학회 2024 Gut and Liver Vol.18 No.4

Background/Aims: Low educational attainment is a well-established risk factor for nonalcoholic fatty liver disease (NAFLD) in developed areas. However, the association between educational attainment and the risk of NAFLD is less clear in China. Methods: A cross-sectional study including over 200,000 Chinese adults across mainland China was conducted. Information on education level and lifestyle factors were obtained through standard questionnaires, while NAFLD and advanced fibrosis were diagnosed using validated formulas. Outcomes included the risk of NAFLD in the general population and high probability of fibrosis among patients with NAFLD. Logistic regression analysis was employed to estimate the risk of NAFLD and fibrosis across education levels. A causal mediation model was used to explore the potential mediators. Results: Comparing with those receiving primary school education, the multi-adjusted odds ratios (95% confidence intervals) for NAFLD were 1.28 (1.16 to 1.41) for men and 0.94 (0.89 to 0.99) for women with college education after accounting for body mass index. When considering waist circumference, the odds ratios (95% CIs) were 0.94 (0.86 to 1.04) for men and 0.88 (0.80 to 0.97) for women, respectively. The proportions mediated by general and central obesity were 51.00% and 68.04% for men, while for women the proportions were 48.58% and 32.58%, respectively. Furthermore, NAFLD patients with lower educational attainment showed an incremental increased risk of advanced fibrosis in both genders. Conclusions: In China, a low education level was associated with a higher risk of prevalent NAFLD in women, as well as high probability of fibrosis in both genders. (Gut Liver 2024;18:719-728)

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Yemen’s Cholera Epidemic Is a One Health Issue

Qin Xiang Ng,Michelle Lee Zhi Qing De Deyn,Wayren Loke,Wee Song Yeo 대한예방의학회 2020 Journal of Preventive Medicine and Public Health Vol.53 No.4

Yemen has been faced with the worst cholera epidemic of modern times, with more than 1 million suspected cases and 3000 deaths at the time of writing. This problem is largely due to the longstanding civil war between pro-government forces and the Houthi armed movement, which has severely damaged already vulnerable sanitation and healthcare facilities and systems in the country. It is further compounded by a dire lack of basic amenities, chronic malnutrition, and unfavourable weather conditions. Another contributory component may be aerial transfer by cholera-infected chironomid insects. To contain the spread of cholera in Yemen, a nation-wide armistice should be negotiated, and national and local committees must be convened to coordinate efforts on the ground. Community isolation facilities with proper sanitation, reliable disposal systems, and a clean water supply should be set up to isolate and treat sick patients. The continuity of vaccination programmes should be ensured. Public health campaigns to educate local communities about good hygiene practices and nutrition are also necessary. The One Health paradigm emphasizes a multi-sectoral and transdisciplinary understanding and approach to prevent and mitigate the threat of communicable diseases. This paradigm is highly applicable to the ongoing cholera crisis in Yemen, as it demands a holistic and whole-of-society approach at the local, regional, and national levels. The key stakeholders and warring parties in Yemen must work towards a lasting ceasefire during these trying times, especially given the extra burden from the mounting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak worldwide.