Interactions of Strawberry with Fungus Pathology and New Germplasm Enhancement in SAAS-FFRI in China

Qing-Hua Gao 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

Microstructure and Properties of Er - SiOX Films Synthesized by Ion Beam Assisted Deposition

Gao Song Duan,Shu Qing Zheng,Xiao Juan Zhang,Qing Yu,Wang Liang 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.2

Er doped SiO_x films have been synthesized by ion beam assisted deposition (IBAD). The morphology and microstructure of films and their annealing behaviors have been examined by using scanning electron microscopy and x-ray diffraction. The composition and properties of films have been systematically investigated.

Neutrophil Count and the Inflammation-based Glasgow Prognostic Score Predict Survival in Patients with Advanced Gastric Cancer Receiving First-line Chemotherapy

Li, Qing-Qing,Lu, Zhi-Hao,Yang, Li,Lu, Ming,Zhang, Xiao-Tian,Li, Jian,Zhou, Jun,Wang, Xi-Cheng,Gong, Ji-Fang,Gao, Jing,Li, Jie,Li, Yan,Shen, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Purpose: To explore the value of systemic inflammatory markers as independent prognostic factors and the extent these markers improve prognostic classification for patients with inoperable advanced or metastatic gastric cancer (GC) receiving palliative chemotherapy. Methods: We studied the prognostic value of systemic inflammatory factors such as circulating white blood cell count and its components as well as that combined to form inflammation-based prognostic scores (Glasgow Prognostic Score (GPS), Neutrophil-Lymphocyte Ratio (NLR), Platelet Lymphocyte Ratio (PLR), Prognostic Index (PI) and Prognostic Nutritional Index (PNI)) in 384 patients with inoperable advanced or metastatic gastric cancer (GC) receiving first-line chemotherapy. Univariate and multivariate analyses were performed to examine the impact of inflammatory markers on overall survival (OS). Results: Univariate analysis revealed that an elevated white blood cell, neutrophil and/or platelet count, a decreased lymphocyte count, a low serum albumin concentration, and high CRP concentration, as well as elevated NLR/PLR, GPS, PI, PNI were significant predictors of shorter OS. Multivariate analysis demonstrated that only elevated neutrophil count (HR 3.696, p=0.003) and higher GPS (HR 1.621, p=0.01) were independent predictors of poor OS. Conclusion: This study demonstrated elevated pretreatment neutrophil count and high GPS to be independent predictors of shorter OS in inoperable advanced or metastatic GC patients treated with first-line chemotherapy. Upon validation of these data in independent studies, stratification of patients using these markers in future clinical trials is recommended.

Ram semen preserved at 0℃ with soybean lecithin Tris-based extender substituted for egg yolk

Zhao, Jian-qing,Xiao, Guo-liang,Zhu, Wen-liang,Fang, Di,Li, Na,Han, Chun-mei,Gao, Qing-hua Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.2

Objective: The present study evaluated the preservation of ram semen at 0℃ using soybean lecithin with a Tris-fructose extender. Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0℃ in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination. Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05). Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0℃ with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

OrMKK3 Influences Morphology and Grain Size in Rice

Ying Hua Pan,Li Jun Gao,Yun Tao Liang,Yan Zhao,Hai Fu Liang,Wei Wei Chen,Xing Hai Yang,Dong Jin Qing,Ju Gao,Hao Wu,Juan Huang,Wei Yong Zhou,Cheng Cui Huang,Gao Xing Dai,Guo Fu Deng 한국식물학회 2023 Journal of Plant Biology Vol.66 No.3

Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from Oryza officinalis Wall. ex Watt encoded by OrMKK3. Different alternative splicing variants occurred in OrMKK3. Green fluorescent protein (GFP)–OrMKK3 fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of OrMKK3 influenced the expression levels of the grain size-related genes SMG1, GW8, GL3, GW2, and DEP3. Phylogenetic analysis showed that OrMKK3 is well conserved in plants while showing large amounts of variation between indica, japonica, and wild rice. In addition, OrMKK3 slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, OrMKK3, influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.

Sputasol (Dithiothreitol 0.54%) Improves the Detection of Human Papillomaviruses Using the Cobas 4800 System

Qing-Yong Wang,He-Rui Zhang,Yu Gao,Rong-Hai Li,Xiao-Hong Shang 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.5

Dear Editor, Persistent infection with human papillomavirus (HPV) is the main cause of cervical cancer. Screening of cervical specimens for high-risk human papillomaviruses (HR-HPV) has become a routine part of patient care in most countries. The Roche cobas 4800 system (Roche Molecular Systems Inc., Pleasanton, CA, USA), a fully automated system that uses in vitro qualitative real-time PCR technology, displays high sensitivity and specificity for HR-HPV detection [1]. However, a small proportion of invalid results may occur in specimens showing mucopurulent discharge because of pipetting errors, clot formation, or failed amplification, which could cause a delay in the results and lead to additional waiting time for patients and extra work hours for staff, although most cases are resolved by vortexing the specimens and retesting. To the best of our knowledge, no researcher has tried to overcome this problem by pretreating specimens with an appropriate concentration of Sputasol (Oxoid Ltd., Basingstoke, United Kingdom); Sputasol (dithiothreitol 1.4%) has been used to aid culturing and DNA extraction from sputum specimens in some laboratories [2]. Here, we propose a standard method to improve HR-HPV detection without affecting the performance of the cobas 4800 HPV system.

Research on the Model, Structure and Characteristics of a New Vibration Generator

Qing-Xin Zhang,Li Yu,Yun-Hong Gao,Lu-Ping Wang 한국전기전자재료학회 2016 Transactions on Electrical and Electronic Material Vol.17 No.6

The vibrational energy is prevalent in the natural environment, which is studied by energy researchers as a new energyresource in recent years. Vibration generation utilizes electromagnetic induction technology, piezoelectric technologyand certain characteristics of smart materials to convert mechanical energy into electrical energy. In this paper, anew method of using MSMA (magnetic shape memory alloy) to generate electricity is proposed and the principleof generating electricity is demonstrated. Martensitic variants and magnetic domain characteristics of MSMA areanalyzed. Combining with Gibbs free energy function thermal theory, the mathematics model of MSMA vibrationgenerator is established. The basic structure of MSMA vibration generator is designed and simulation is done toanalyze that the effects of generator output voltage when the input amplitude and frequency of vibration stresschange. The simulation experiments verify the feasibility of using MSMA to make the micro vibration generators andthe correctness of the mathematical model, which lays a good foundation for the further research and application ofMSMA vibration generator.

Expression profile and estrogenic regulation of Amh during gonadal sex differentiation in northern snakehead (Channa argus)

Qing Luo,Mi Ou,Jian Zhao,Haiyang Liu,Dandan Gao,Yanduo Wu,Lanlan Zhang,Kunci Chen 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background Anti-Müllerian hormone (Amh) plays a critical role in both early sex determination and later gonad development in vertebrate species. However, it remains unknown in northern snakehead (Channa argus), which is economically important freshwater fish with sexual dimorphism. Objective This study aimed to identify the expression profiles and estrogenic regulation of CaAmh during gonadal sex differentiation in C. argus. Methods The cDNA and genomic DNA sequences of CaAmh were identified by PCR and RACE techniques. The expression patterns of CaAmh were detected by qRT-PCR during the gonadal sex differentiation and after 17α-ethinyloestradiol (EE2) treatments. Results CaAmh is composed of seven exons and six introns, and its full-length cDNA is 2413 bp in length, with 1635 bp open reading frame (ORF) that encodes a 544 amino acid protein. Tissues expression patterns revealed that CaAmh display the highest expression in testis of XY males (40.36 folds, p < 0.01). The spatio-temporal expression patterns during gonadal sex differentiation indicated that CaAmh expression differed between XX females and XY males at 30 day after hatching (dah), and reached to the peak (36.03 folds, p < 0.01) at 90 dah in XY gonads. However, CaAmh expression in XX gonads remained low throughout the sampling period. Furthermore, CaAmh expression in the gonads (ovaries) of the sex-reversed XY fish (XY-F) by the administration of estrogen EE2 was downregulated to low level, similar to that in ovaries of normal XX females (XX-F). Conclusions These results show that Amh plays a critical role in testicular differentiation of C. argus and it is apparently modulated by estrogens in this species.

Dynamic and Damage Characteristics of Mortar Composite under Impact Load

Qing Li,Tong-de Zhao,Chen Huang,Bing-bing Yu,Zheng-Hua Gao,Kai Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.3

In the process of underground construction, it is common to spray concrete on the working surface, which will form a concrete-rock composite structure, to achieve the expected reinforcement effect. Compared with pure concrete or rock structures, composite structures have different mechanical properties, energy evolution and damage characteristics. Understanding the dynamic mechanics and damage characteristics of composite structures under impact loads is important to the safety of underground engineering construction. First, dynamic compression tests on different grades of mortar-sandstone composite specimens were carried out by using a Hopkinson pressure bar system to discuss the effect of impact velocities on the mechanical properties and failure modes of composite specimens. The results show that the dynamic peak stress, strain energy density and fragmentation block of the mortar-sandstone composite have an obvious strain rate effect. With an increase in impact velocity, the internal cracks of the sample do not have sufficient time to expand, and the energy absorbed by the sample leads to the continuous initiation of new cracks in the sample, resulting in an increasing damage degree of composite specimens. The increase in mortar strength can significantly increase the peak stress, improve the load-bearing capacity of the sample, and reduce the degree of sample damage. At the same impact velocity, the fragmentation degree of the mortar sample is more severe than that of sandstone, and the mortar sample is broken into powder debris, while the sandstone is broken into fragments. In addition, according to the failure properties of the composite sample, the mortar and sandstone samples are described by different damage models, and the dynamic damage constitutive model of the mortar-sandstone composite is established based on the overlay principle. The dynamic test results were fitted. The research shows that the fitting curves of the model are well fitted with the test curves and that the model accurately reflects the changes in the mechanical parameters of the composite specimens.

Embryological and genetic evidence of amphimixis and apomixis in Boehmeria tricuspis

Qing Tang,Gonggu Zang,Lining Zhao,Chaohua Cheng,Zhixue Dong,Chunsheng Gao 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

Apomixis is a widespread alternative mode of sexual reproduction resulting in offspring that are genetically identical to the maternal plant. Boehmeria tricuspis (Hance) Makino is a perennial, wind-pollinated, herbaceous plant in the nettle family Urticaceae. The diploid B. tricuspis is monoecious but the triploid B. tricuspis is gynoecious, bearing female inflorescences only. Apomixis in B. tricuspis was first reported 50 years ago, but the mode of apomixis in the species has not been described yet. Here, we provide embryological observations of the embryo sac formation proving that triploid B. tricuspis reproduced apomictically following the Antennaria type of diplospory, and that the diploid individuals were the sexual genotype with the classical Polygonum-type maturation pattern of embryo sac development. A subsequent flow cytometry seed screen (FCSS) showed that the triploids were obligate apomicts with autonomous endosperm development, and the diploids reproduced sexually. In addition, a progeny test by molecular marker assays further demonstrated the above results.