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        Panaxadiol saponins treatment caused the subtle variations in the global transcriptional state of Asiatic corn borer, Ostrinia furnacalis

        Liu, Shuangli,Xu, Yonghua,Gao, Yugang,Zhao, Yan,Zhang, Aihua,Zang, Liansheng,Wu, Chunsheng,Zhang, Lianxue The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

        Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused huge economic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used for the control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypic pesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins (PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary to reveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assembly method. Results: PDS treatment could cause the changes of many gene expressions which regulate its signal pathways. The genes in peroxisome proliferator-activated receptor (PPAR) signaling pathway were significantly downregulated, and then, the downstream fatty acid degradation pathway had also been greatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACB is because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR, and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL, SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradation pathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression of genes associated with catalytic activity, such as cytochrome P450 and other similar genes.

      • KCI등재
      • KCI등재

        Panaxadiol saponins treatment caused the subtle variations in the global transcriptional state of Asiatic corn borer, Ostrinia furnacalis

        Shuangli Liu,Yonghua Xu,Yugang Gao,Yan Zhao,Aihua Zhang,Liansheng Zang,Chunsheng Wu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

        Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused hugeeconomic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used forthe control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypicpesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins(PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary toreveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assemblymethod. Results: PDS treatment could cause the changes of many gene expressions which regulate its signalpathways. The genes in peroxisome proliferatoreactivated receptor (PPAR) signaling pathway weresignificantly downregulated, and then, the downstream fatty acid degradation pathway had also beengreatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACBis because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR,and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL,SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradationpathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression ofgenes associated with catalytic activity, such as cytochrome P450 and other similar genes.

      • KCI등재

        Embryological and genetic evidence of amphimixis and apomixis in Boehmeria tricuspis

        Qing Tang,Gonggu Zang,Lining Zhao,Chaohua Cheng,Zhixue Dong,Chunsheng Gao 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

        Apomixis is a widespread alternative mode of sexual reproduction resulting in offspring that are genetically identical to the maternal plant. Boehmeria tricuspis (Hance) Makino is a perennial, wind-pollinated, herbaceous plant in the nettle family Urticaceae. The diploid B. tricuspis is monoecious but the triploid B. tricuspis is gynoecious, bearing female inflorescences only. Apomixis in B. tricuspis was first reported 50 years ago, but the mode of apomixis in the species has not been described yet. Here, we provide embryological observations of the embryo sac formation proving that triploid B. tricuspis reproduced apomictically following the Antennaria type of diplospory, and that the diploid individuals were the sexual genotype with the classical Polygonum-type maturation pattern of embryo sac development. A subsequent flow cytometry seed screen (FCSS) showed that the triploids were obligate apomicts with autonomous endosperm development, and the diploids reproduced sexually. In addition, a progeny test by molecular marker assays further demonstrated the above results.

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