Chemical Constituents from the Aerial Parts of Isodon coetsa and their Cytotoxicity

Wei Zhao,Jian Xin Pu,Xue Du,Yong Zhao,Fei He,Hai Bo Zhang,Yong Bo Xue,Wei Lie Xiao,Han Dong Sun,Ying Li Wu,Guo Qiang Chen 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

Three new compounds (1-3), including a neolignan, a triterpenoid, and a diterpenoid, together with twenty known compounds (4-23), were isolated from the aerial parts of Isodon coetsa. Their structures and relative configurations were elucidated on the basis of spectroscopic data. Compounds 1, 3, 5-9, 11-13, 16-17, and 19-23 were evaluated for their cytotoxicity against HT-29, BEL-7402, and SK-OV-3 human tumor cell lines. Compound 7 showed significant inhibitory effects on all three types of cells, with IC50 values of 2.52, 3.06, 2.14 μM, respectively.

Numerical simulation on mining effect influenced by a normal fault and its induced effect on rock burst

Jiang, Jin-Quan,Wang, Pu,Jiang, Li-Shuai,Zheng, Peng-Qiang,Feng, Fan Techno-Press 2018 Geomechanics & engineering Vol.14 No.4

The study of the mining effect influenced by a normal fault has great significance concerning the prediction and prevention of fault rock burst. According to the occurrence condition of a normal fault, the stress evolution of the working face and fault plane, the movement characteristics of overlying strata, and the law of fault slipping when the working face advances from footwall to hanging wall are studied utilizing UDEC numerical simulation. Then the inducing-mechanism of fault rock burst is revealed. Results show that in pre-mining, the in situ stress distribution of two fault walls in the fault-affected zone is notably different. When the working face mines in the footwall, the abutment stress distributes in a "double peak" pattern. The ratio of shear stress to normal stress and the fault slipping have the obvious spatial and temporal characteristics because they vary gradually from the higher layer to the lower one orderly. The variation of roof subsidence is in S-shape which includes slow deformation, violent slipping, deformation induced by the hanging wall strata rotation, and movement stability. The simulation results are verified via several engineering cases of fault rock burst. Moreover, it can provide a reference for prevention and control of rock burst in a fault-affected zone under similar conditions.

Heterologous overexpression of the Arabidopsis SnRK2.8 gene enhances drought and salt tolerance in Populus × euramericana cv ‘Nanlin895’

Hui Wei,Ali Movahedi,Chen Xu,Pu Wang,Weibo Sun,Tongming Yin,Qiang Zhuge 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

The SnRK (sucrose non-fermenting-1 related protein kinase) families respond to salt tolerance, nutritional stress, disease stress, and carbon metabolic regulation. In this study, the SnRK2.8 gene was cloned from Arabidopsis thaliana and nominated AtSnRK2.8. Bioinformatics characterization of AtSnRK2.8 showed that it contained an ATP binding site and a serine/ threonine protein kinase binding site at the N-terminal catalytic domain. In addition, the AtSnRK2.8 protein contained special domain I at the C-terminal catalytic domain. Real-time polymerase chain reaction (PCR) results showed that the AtSnRK2.8 gene was expressed in all tested tissues and was highly expressed in seeds. Our analyses proved that the AtSnRK2.8 gene expression was upregulated by salt and drought stresses. The recombinant AtSnRK2.8 protein was expressed using a pET-28a system and purified with HisTrap HP affinity columns. The recombinant AtSnRK2.8 protein has the properties of a protein kinase. The results of overexpressing AtSnRK2.8 in Nanlin895 poplar showed that the transgenic poplar plants had a better ability to resist drought and salt stress compared to the wild type, and adapted well to drought and a high-salt environment.

MLH1 Polymorphisms and Cancer risk: a Meta-analysis Based on 33 Case-control Studies

Xu, Jia-Li,Yin, Zhi-Qiang,Huang, Ming-De,Wang, Xie-Feng,Gao, Wen,Liu, Ling-Xiang,Wang, Rong-Sheng,Huang, Pu-Wen,Yin, Yong-Mei,Liu, Ping,Shu, Yong-Qian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Objective: Cumulative evidence suggests that MLH1, the key component in the mismatch pathway, plays an important role in human cancers. Two potential functional polymorphisms (-93G>A and I219V) of MLH1 have been implicated in cancer risk. The aim of this meta-analysis was to summarize the evidence for associations. Methods: Eligible studies were identified by searching the electronic literature PubMed, ScienceDirect and Embase databases for relevant reports and bibliographies. Studies were included if of case-control design investigating MLH1 polymorphisms (-93G>A and I219V) and cancer risk with sufficient raw data for analysis. Odds ratios (OR) and 95% confidence intervals (95% CI) were used to evaluate the strength of associations. Results: Our meta-analysis from 33 published case-control studies showed the variant A allele of -93G>A polymorphism to be associated with increased risk in all genetic models (AA vs. GG: OR = 1.22, 95% CI: 1.03-1.44), especially among non-Asians (AA vs. GG: OR = 1.28, 95% CI: 1.04-1.58). For the I219V polymorphism, however, there was no main effect associated with overall cancer risk in any genetic model. Conclusions: The meta-analysis suggested that the MLH1 -93G>A polymorphism may be a biomarker of cancer susceptibility. Large sample association studies and assessment of gene-to-gene as well as gene-to-environment interactions are required to confirm these findings.

Superhydrophobic and smart MgAl-LDH anti-corrosion coating on AZ31 Mg surface

Manyi Huang,Guangming Lu,Jibin Pu,Yujie Qiang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

Layered double hydroxides (LDHs) have been widely used as ‘‘smart” containers in the field of metal corrosionprotection, and have broad industrial prospects. It is environmentally friendly and feasible to usealiphatic carboxylates and corresponding acids as substitutes for harmful corrosion inhibitors. However,previous studies rarely involved comparing the anti-corrosion mechanisms of different aliphatic acidsmodified on the LDH surface, and the durability of the prepared coatings also needs to be improved. Inthis work, MgAl-LDH laminates were grown in situ on AZ31 substrates by a hydrothermal method,and then modified by sodium laurate (SL) and sodium dodecylbenzene sulfonate (SDBS). Due to the physicalbarrier effect of the LDH layers, the spatial repulsion effect of the air film and the ion exchange reactionsin the interlayer galleries, the functional coatings prepared exhibit smart and superior anticorrosionperformance on the magnesium substrates in 3.5 wt.% NaCl solution. Compared with LDHSDBS-8 coating, the obtained superhydrophobic LDH-SL-8 coating shows more excellent long-term corrosionprotection owing to the stronger intercalation capacity.

Profile of disposition, tissue distribution and excretion of the novel anti-human immunodeficiency virus (HIV) agent W-1 in rats

Ying-Yuan Lu,Xiao-Wei Wang,Xin Wang,Wen-Bing Dai,Qiang Zhang,Pu Li,Ya-Qing Lou,Chuang Lu,Jun-Yi Liu,Guo-Liang Zhang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

The purpose of this study was to characterize the disposition, distribution, excretion and plasma protein binding of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W- 1) in rats. Concentrations of W-1 within biological samples were determined using a validated high performance liquid chromatography method. The plasma protein binding of W-1 was examined by equilibrium dialysis method. After oral administration of W-1 (50, 100 and 200 mg/kg, respectively) in self-microemulsifying drug delivery system formulation, the pharmacokinetic parameters of W-1 were as follows: the peak plasma concentrations (Cmax) were 0.42, 1.50 and 2.55 μg/mL, the area under the curve (AUC0-t) were 0.89, 2.27 and 3.96 lg/h mL and the plasma half-life (t1/2) were 5.15, 3.77 and 3.77 h, respectively. Moreover, the prototype of W-1 was rapidly and extensively distributed into fifteen tissues, especially higher concentrations were detected in intestine, stomach and liver, respectively. The plasma protein binding of W-1 in rat, beagle dog and human were in the range of 97.96–99.13 %. This study suggested that W-1 has an appropriate pharmacokinetics in rats, such as rapid absorption, moderate clearance, and rapid distribution to multiple tissues. Those properties provide important information for further development W-1 as an anti-HIV-1 drug candidate.

Estimation of Growth Curves and Suitable Slaughter Weight of the Liangshan Pig

Jia Luo,Huaigang Lei,Linyuan Shen,Runlin Yang,Qiang Pu,Kangping Zhu,Mingzhou Li,Guoqing Tang,Xuewei Li,Shunhua Zhang,Li Zhu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9

The Liangshan pig is a traditional Chinese small-sized breed; it has a relatively long feeding period and low meat production ability but superior meat quality. This study utilized three non-linear growth models (Von Bertalanffy, Gompertz, and logistic) to fit the growth curve of Liangshan pigs from an unselected, random-bred pig population and estimate the pigs most suitable slaughter weight. The growth development data at 20 time points of 275 Liangshan pigs (from birth to 250 d) were collected. To analyze the relative gene expression related to development, seven slaughter weight phases (50, 58, 66, 74, 82, 90, and 98 kg) (20 pigs per phase) were examined. We found that the Liangshan pig growth curve fit the typical S-curve well and that their growth turning point was 193.4 days at a weight of 62.5 kg, according to the best fit Von Bertalanffy model based on the goodness of fit criteria. Furthermore, we estimated that the most suitable slaughter weight was 62.5 to 74.9 kg based on the growth curve and the relative expression levels of growth-related genes.

Intersubband Transitions in Nonpolar GaN-based Resonant Phonon Depopulation Multiple-Quantum Wells for Terahertz Emissions

Ya-Feng Song,Xiong-Xiong Kong,Wei-Bin Tang,Zhong-Qiang Suo,Huan Zhang,Chen-Yang Li,Qian Jia,Cai-Xia Xue,Yan-Wu Lu,Chao-Pu Yang 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.11

We investigate the polarization effect in intersubband transitions in polar and nonpolar GaN-based multiple-quantum well (MQW) structures for terahertz (THz) emissions by using systematic comparisons and design a nonpolar GaN/Al$_{0.2}$Ga$_{0.8}$N two-well-based MQW structure with an emitting photon of 7.27 THz (30.07 meV). Its lower energy separation (92.7 meV) matches the resonant phonon depopulation condition for better population inversion. It shows a lower threshold current density $J\rm_{th}$ at all temperatures (1.548 kA/cm$^2$ at 90 K) and a higher output power of up to 86.1 mW at 5.8 K and 33.6 mW at 100 K. Our results for the polar GaN MQW are very close to the experimental data in the literature. We find that the $J\rm_{th}$ of the nonpolar GaN MQW increases more slowly than that of the polar GaN MQW as temperature increases, indicating the nonpolar GaN MQW may be a worth-trying direction for improving the operation temperature. These results can provide meaningful references for the design and fabrication of nonpolar GaN-based THz MQW or quantum cascade structures.

Enhanced anticorrosion of methyl acrylate by covalent bonded N-alkylpyridinium bromide for X70 steel in 5 M HCl

Guangqiang Xia,Xiaohui Jiang,Limei Zhou,Yinwen Liao,Ming Duan,Hu Wang,Qiang Pu,Jie Zhou 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-

Methyl acrylate connects covalently with alkylpyridinium bromide to build N-alkyl-3-(2-methox-ycarbonyl-vinyl)pyridinium bromide (MPA-n, n = 8–14). MPA-n inhibited X70 steel from corrosion in5 M HCl with inhibition efficiency of 98% and the inhibition capacity was in the order of MPA-14 > MPA-12 > MPA-10 > MPA-8. Quantum chemical calculation unveiled that MPA-n adsorbed on iron surface byO atom in MA moiety offering electrons to d orbital of iron and by p* orbital of pyridinium acceptingelectrons from iron, and MPA-n with longer alkylchain possessed higher EHOMO, lower DE, largermolecular volume and dipole moment, all suggesting the same corrosion inhibition order as that inexperiments

Estimation of Growth Curves and Suitable Slaughter Weight of the Liangshan Pig

Luo, Jia,Lei, Huaigang,Shen, Linyuan,Yang, Runlin,Pu, Qiang,Zhu, Kangping,Li, Mingzhou,Tang, Guoqing,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The Liangshan pig is a traditional Chinese small-sized breed; it has a relatively long feeding period and low meat production ability but superior meat quality. This study utilized three non-linear growth models (Von Bertalanffy, Gompertz, and logistic) to fit the growth curve of Liangshan pigs from an unselected, random-bred pig population and estimate the pigs most suitable slaughter weight. The growth development data at 20 time points of 275 Liangshan pigs (from birth to 250 d) were collected. To analyze the relative gene expression related to development, seven slaughter weight phases (50, 58, 66, 74, 82, 90, and 98 kg) (20 pigs per phase) were examined. We found that the Liangshan pig growth curve fit the typical S-curve well and that their growth turning point was 193.4 days at a weight of 62.5 kg, according to the best fit Von Bertalanffy model based on the goodness of fit criteria. Furthermore, we estimated that the most suitable slaughter weight was 62.5 to 74.9 kg based on the growth curve and the relative expression levels of growth-related genes.