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Jia-Qi Chu,Xu-Min Hu,김명철,박창식,전무형 한국미생물학회 2009 The journal of microbiology Vol.47 No.5
Three indirect enzyme-linked immunosorbent assays (iELISA) based on the North American like (NA-like), European like (EU-like) and co-expressed NA- and EU-like recombinant nucleocapsid proteins (N-protein) of porcine reproductive and respiratory syndrome virus (PRRSV) were validated for the detection of the antibodies in porcine sera. A total of 422 serum samples from unvaccinated pigs were tested. The cut-off value was optimized by a two-graph receiver operating characteristics analysis at a 95% confidence level. This assay was validated with Western blot analysis and IDEXX HerdChek™ ELISA. Cross-reactivity results showed that iELISA was PRRSV-specific. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were less than 10%. The results indicate that iELISA is simpler to produce and perform, time-saving and suitable for large scale surveys of PRRSV infection at low cost, and is potentially useful to evaluate the efficiency of various vaccines against PRRSV.
Electronic and optical properties of graphane, silicane, MoS2 homo-bilayers and hetero-bilayers
Jia-Qi Hu,Lin-Han Xu,Shun-Qing Wu,Zi-Zhong Zhu 한국물리학회 2019 Current Applied Physics Vol.19 No.11
The electronic and optical properties of graphane, silicane and MoS2 bilayers, as well as the graphane/MoS2 and silicane/MoS2 hetero-bilayers, are calculated by the first-principles method. The interlayer interactions of all the bilayer systems are shown to be mainly van der Waals. Both the graphane/MoS2 and silicane/MoS2 heterobilayers belong to the type-II heterostructure, which can be utilized in photo-voltaic devices due to the efficient spatial separation of electrons and holes. For optical properties, the distinctions for the imaginary parts of the dielectric function 2 ( ) between the monolayer and bilayer systems for both the graphane and silicane are more evident in electric vector E||z. However, the differences between 2 ( ) of the monolayer and bilayer MoS2 materials are more significant in E||x. Broader light absorption ranges of the hetero-bilayers are reached, which can also improve the charge separation of the electron-hole pairs.
Jia-Qi Chu,박창식,유명조,전무형,김명철 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.3
To investigate the genotypic diversity of the porcine reproductive and respiratory syndrome viruses (PRRSV) in Korea, we examined 92 clinical samples from three provinces by RT-PCR and a nested PCR, and the complete open-reading frame 7 (ORF 7) sequences of 15 samples selected from 72 PCR-positive specimens were analyzed. When we compared nucleotide (amino acid) sequences of 80 isolates from Korea and overseas countries, the sequences of 7 samples belonged to North American (NA)-genotype, and those of 8 samples, to European (EU)-genotype. The nucleotide (amino acid) identities between two genotypes were 63.7% (59.8%) to 65.1% (63.1%). When compared with NA prototype VR-2332, the 7 strains of NA-genotype shared 89.8% (93.6%) to 91.2% (96.0%) identity of nucleotide (amino acid) sequence. The 8 strains of EU-type shared 93.6% (92.3%) to 94.3% (93.8%) identity of nucleotide (amino acid) sequence as compared to EU prototype Lelystad. In phylogenetic tree analysis by neighborjoining method, all of the 8 EU-type strains were clustered into group 4 distinct from EU-prototype Lelystad (group 1). In NA-genotype, 24 domestic isolates reported previously and the 7 strains of NA-type determined in this study were clustered into group 1, while US prototype VR 2332 was classified into different group (group 2). These results suggest that emergence of EU-genotype and the dual-infection of NA- and EU-genotypes may be prevalent in the pig farms in Korea. The high degree of genetic diversity of field PRRSVs should be taken into consideration for control and preventive measures.
Qi Jia,Gangui Yan,Wenbo Hu,Kan Liu,Junxi Wang 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.1
Aiming at the sub-synchronous oscillation (SSO) problem that direct-drive permanent magnetic synchronous generator (PMSG) based wind farm under low operating conditions connects to the grid, based on the idea of graphical block modeing, the wind turbine generation (WTG), collector circuit and grid are regarded as discrete components. On the basis of establishing detailed models of each component, two linearized models of direct-drive PMSG based wind farm under different collector circuit topological structure is constructed. Wind farm impedance model is constructed based on state space equation of linearized model. The infl uences of current inner loop control parameters, grid strength, operating conditions on SSO characteristics are analysed. The diff erence between the two models in the study of SSO is compared. Finally, a time-domain simulation model of direct drive PMSG based wind farm connected to grid is built in EMTDC/PSCAD and the validity of the theoretical analysis is verifi ed.