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RISS 인기검색어
- 검색키워드
- 저자 : Ning Shi (검색결과 61 건)
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The modal characteristics of non-uniform multi-span continuous beam bridges
Shi, Lu-Ning,Yan, Wei-Ming,He, Hao-Xiang Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.5
According to the structure characteristics of the non-uniform beam bridge, a practical model for calculating the vibration equation of the non-uniform beam bridge is given and the application scope of the model includes not only the beam bridge structure but also the non-uniform beam with added masses and elastic supports. Based on the Bernoulli-Euler beam theory, extending the application of the modal perturbation method and establishment of a semi-analytical method for solving the vibration equation of the non-uniform beam with added masses and elastic supports based is able to be made. In the modal subspace of the uniform beam with the elastic supports, the variable coefficient differential equation that describes the dynamic behavior of the non-uniform beam is converted to nonlinear algebraic equations. Extending the application of the modal perturbation method is suitable for solving the vibration equation of the simply supported and continuous non-uniform beam with its arbitrary added masses and elastic supports. The examples, that are analyzed, demonstrate the high precision and fast convergence speed of the method. Further study of the timesaving method for the dynamic characteristics of symmetrical beam and the symmetry of mode shape should be developed. Eventually, the effects of elastic supports and added masses on dynamic characteristics of the three-span non-uniform beam bridge are reported.
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Ning-Ning Dou,Hao-lin Wang,Shao-Zhen Hu,Zheng-Nan Huang,Jun Zhong,Shi-Ting Li 대한척추신경외과학회 2023 Neurospine Vol.20 No.3
Objective: Although endoscopic drill has the advantages in manipulation and hemostasis, whose low efficiency and blurred vision reduce the efficacy of lumbar endoscopic unilateral laminotomy with bilateral decompression (LE-ULBD). The present study was designed to evaluate the safety and efficacy of full-visualized trephine/osteotome in the LE-ULBD surgery for severe lumbar stenosis. Methods: Fifty-seven severe lumbar stenosis patients who underwent LE-ULBD between January 2020 to January 2023 were enrolled, who were divided into drill and visualized trephine groups. The medical records including demographics, operative duration, intraoperative electrophysiological findings, postoperative hospital stay or hospital stay, postoperative outcomes and complications were retrospectively reviewed and analyzed. Results: A total of 57 patients included 15 in drill and 42 in trephine group were enrolled in the study. There was significant difference in the pre- and postoperative visual analogue scale and Oswestry Disability Index scores in both groups (p < 0.05). The mean operative duration in the trephine group (101.05 ± 12.18 minutes) was shorter than that in the drill group (134.67 ± 9.68 minutes) (p < 0.05). There was no statistical difference between the 2 groups in electrophysiological monitoring, posthospital stays, postoperative outcomes and complications. Abnormal free-electromyography (EMG) were recorded in 2 (13.3%) and 5 patients (11.9%) in the drill and trephine group. Intraoperative somatosensory evoked potential changes occurred in 3 (20%) and 3 patients (7.1%) in the drill and trephine group and all patients recovered immediately when surgery ended. No serious complications and recurrence occurred in all the patients. Conclusion: Full-visualized trephine/osteotome has been approved to be convenient, safe and efficient in our study, which combined with translaminar inside-out technique and EMG monitoring especially free-EMG may offer a new choice in LE-ULBD surgery for lumbar stenosis patients.
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Ning-Ning Ji,Zhi-Qiang Shi,Ren-Gao Zhao,Ze-Bao Zheng,Zhi-Feng Li 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.4
A novel Schiff base N-(2,4-dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine has been synthesized and structurally characterized by X-ray single crystal diffraction, elemental analysis, IR spectra and UV-vis spectrum. The crystal belongs to monoclinic with space group P21/n. The molecules are connected via intermolecular O-H···O hydrogen bonds into 1D infinite chains. The crystal structure is consolidated by the intramolecular N-H···O hydrogen bonds. weak intermolecular C-H···O hydrogen bonds link the molecules into intriguing 3D framework. Furthermore, Density functional theory (DFT) calculations of the structure, stabilities, orbital energies, composition characteristics of some frontier molecular orbitals and Mulliken charge distributions of the title compound were performed by means of Gaussian 03W package and taking B3LYP/6-31G(d) basis set. The time-dependent DFT calculations have been employed to calculate the electronic spectrum of the title compound, and the UV-vis spectra has been discussed on this basis. The results show that DFT method at B3LYP/6-31G(d) level can well reproduce the structure of the title compound.
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Ning-Ning Song,Yan Zheng,Shi-Jin E,Duo-Chuan Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.1
A superoxide dismutase (SOD) gene of Thermoascus aurantiacus var. levisporus, a thermophilic fungus, was cloned, sequenced, and expressed in Pichia pastoris and its gene product was characterized. The coding sequence predicted a 231 residues protein with a unique 35 amino acids extension at the N-terminus indicating a mitochondrial-targeting sequence. The content of Mn was 2.46 µg/mg of protein and Fe was not detected in the purified enzyme. The enzyme was found to be inhibited by NaN3, but not by KCN or H2O2. These results suggested that the SOD in Thermoascus aurantiacus var. levisporus was the manganese superoxide dismutase type. In comparison with other MnSODs, all manganese-binding sites were also conserved in the sequence (H88, H136, D222, H226). The molecular mass of a single band of the enzyme was estimated to be 21.7 kDa. The protein was expressed in tetramer form with molecular weight of 68.0 kDa. The activity of purified protein was 2,324 U/mg. The optimum temperature of the enzyme was 55°C and it exhibited maximal activity at pH 7.5. The enzyme was thermostable at 50 and 60°C and the half-life at 80°C was approximately 40 min.
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Ji, Ning-Ning,Shi, Zhi-Qiang,Zhao, Ren-Gao,Zheng, Ze-Bao,Li, Zhi-Feng Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.4
A novel Schiff base N-(2,4-dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine has been synthesized and structurally characterized by X-ray single crystal diffraction, elemental analysis, IR spectra and UV-vis spectrum. The crystal belongs to monoclinic with space group P21/n. The molecules are connected via intermolecular O-$H{\cdots}O$ hydrogen bonds into 1D infinite chains. The crystal structure is consolidated by the intramolecular N-$H{\cdots}O$ hydrogen bonds. weak intermolecular C-$H{\cdots}O$ hydrogen bonds link the molecules into intriguing 3D framework. Furthermore, Density functional theory (DFT) calculations of the structure, stabilities, orbital energies, composition characteristics of some frontier molecular orbitals and Mulliken charge distributions of the title compound were performed by means of Gaussian 03W package and taking B3LYP/6-31G(d) basis set. The time-dependent DFT calculations have been employed to calculate the electronic spectrum of the title compound, and the UV-vis spectra has been discussed on this basis. The results show that DFT method at B3LYP/6-31G(d) level can well reproduce the structure of the title compound.
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The modal characteristics of non-uniform multi-span continuous beam bridges
Lu-ning Shi,Wei-ming Yan,Hao-xiang He 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.5
According to the structure characteristics of the non-uniform beam bridge, a practical model for calculating the vibration equation of the non-uniform beam bridge is given and the application scope of the model includes not only the beam bridge structure but also the non-uniform beam with added masses andelastic supports. Based on the Bernoulli-Euler beam theory, extending the application of the modal perturbation method and establishment of a semi-analytical method for solving the vibration equation of thenon-uniform beam with added masses and elastic supports based is able to be made. In the modal subspace of the uniform beam with the elastic supports, the variable coefficient differential equation that describes the dynamic behavior of the non-uniform beam is converted to nonlinear algebraic equations. Extending the application of the modal perturbation method is suitable for solving the vibration equation of the simply supported and continuous non-uniform beam with its arbitrary added masses and elastic supports. The examples, that are analyzed, demonstrate the high precision and fast convergence speed of the method. Further study of the timesaving method for the dynamic characteristics of symmetrical beam and the symmetry of mode shape should be developed. Eventually, the effects of elastic supports and added masseson dynamic characteristics of the three-span non-uniform beam bridge are reported.
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Effects of moderate pressure on premeability and viability of Saccharomyces cerevisiae cells
Shi-Ru Jia,Na-Chen,Yu-Jie Dai,Chang-Sheng Qiao,Jian-Dong Cui,Bo-Ning Liu 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3
With CO2 and N2 as the pressure media, the effects of the moderate pressure (0.1-1.0MPa) and the holding time on the conductivities of the cell suspension of Saccharomyces cerevisiae CICC1447 and Saccharomyces cerevisiae CICC1339, as well as the absorbances of the supernatant (after centrifuged) at 280 nm (A280) and 260 nm (A260) were determined. The membrane permeability of Saccharomyces cerevisiae CICC1447 increased significantly and the cell leakage was aggravated with the pressure increase. For Saccharomyces cerevisiae CICC1339, the conductivity of the cell suspension, A280 and A260 of the supernatant fluctuated with the pressure increase; as a whole, they increased with pressure. Different from high pressure, a moderate pressure not only remarkably improved the permeability of the yeast cell membrane, but also kept yeast cell viability; moreover, the integrity of the yeast cell membrane could be maintained.
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Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1
Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.
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