LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2

Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.

Treatment of fever with traditional Chinese medicine according to Zheng on cancer patients (based on case reports)

Peng Cao,Lan-Ying Liu,Xue-Ting Cai,Xiao-Ning Wang,Jie-Ge Huo,Zhong-Ying Zhou 셀메드 세포교정의약학회 2012 셀메드 (CellMed) Vol.2 No.2

Fever in cancer patients is often due to the following causes: evil qi and toxity stagnancy, disorders of qi and blood, deficiencies of zang and fu organs, and the disorder of yin and yang. The treatments given to cancer patients with a fever are according to five: (a) Excessive inner heat and toxicants: remove heat and the toxicant, induce purgation. We use Cheng-Qi-Tang plus Qing-Wen-Bai-Du-Yin. (b) Tangle of damp and heat, and qi stagnancy: remove damp and heat, smooth the qi channel. We use Gan-Lu-Xiao-Du-Dan or San-Ren-Tang. (c) Obvious blood and heat stagnancy: remove heat and blood stasis. We use Xue-Fu-Zhu-Yu-Tang. (d) Deficiency of spleen qi, inner heat caused by a yin deficiency: nourish spleen qi and yin to remove the inner heat. We use Bu-Zhong-Yi-Qi-Tang or Xiao-Jian-Zhong-Tang. (e) Prominent yin deficiency and hectic fever: replenish yin and remove inner heat. We use Qing-Hao-Bie-Jia-Tang or Chai-Qian-Mei-Lian-San. The pathogenesis of fever in cancer patients is complicated. We can see both deficiency and excess in one differentiation. Therefore, we must make sure of it, then we can get the most effective treatment.

Analytical solution for natural frequency of monopile supported wind turbine towers

Xue-Ning Rong,Ri-Qing Xu,Heng-Yu Wang,Su-Yang Feng 한국풍공학회 2017 Wind and Structures, An International Journal (WAS Vol.25 No.5

In this study an analytical expression is derived for the natural frequency of the wind turbine towers supported on flexible foundation. The derivation is based on a Euler-Bernoulli beam model where the foundation is represented by a stiffness matrix. Previously the natural frequency of such a model is obtained from numerical or empirical method. The new expression is based on pure physical parameters and thus can be used for a quick assessment of the natural frequencies of both the real turbines and the small-scale models. Furthermore, a relationship between the diagonal and non-diagonal element in the stiffness matrix is introduced, so that the foundation stiffness can be obtained from either the p-y analysis or the loading test. The results of the proposed expression are compared with the measured frequencies of six real or model turbines reported in the literature. The comparison shows that the proposed analytical expression predicts the natural frequency with reasonable accuracy. For two of the model turbines, some errors were observed which might be attributed to the difference between the dynamic and static modulus of saturated soils. The proposed analytical solution is quite simple to use, and it is shown to be more reasonable than the analytical and the empirical formulas available in the literature.

Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.

Adoption of Smart Sustainability Performance Measurement System (SPMS) in Hotels and Variations across Ratings, Reviews, and Operational Efficiency Scores

Ning, Xue,Yim, Dobin,Khuntia, Jiban Smart Tourism Research Center 2021 Journal of smart tourism Vol.1 No.2

Hotels have recently started to implement enterprise information systems to measure and report sustainability indicators in a smart manner. However, a complex ownership structure in a hotel chain prevents full smart systems adoption at the individual property level. This study explores how a smart sustainability performance measurement system (SPMS) for waste management adoption correlates with customer ratings, customer reviews, operational efficiency scores, and between franchised and corporate-managed properties. We derive insights from the secondary data constructed from multiple sources for a large multinational hotel chain hotel. The findings suggest that hotels that adopt SPMS have better operational efficiency scores and more customer reviews. Within the hotels that adopted SPMS, corporate-managed hotels have a lower level of ratings than franchised hotels, but they have higher operational efficiency scores and more reviews. We discuss research implications for the concept of smart tourism and hotel management literature and managerial implications.

Analytical solution for natural frequency of monopile supported wind turbine towers

Rong, Xue-Ning,Xu, Ri-Qing,Wang, Heng-Yu,Feng, Su-Yang Techno-Press 2017 Wind and Structures, An International Journal (WAS Vol.25 No.5

In this study an analytical expression is derived for the natural frequency of the wind turbine towers supported on flexible foundation. The derivation is based on a Euler-Bernoulli beam model where the foundation is represented by a stiffness matrix. Previously the natural frequency of such a model is obtained from numerical or empirical method. The new expression is based on pure physical parameters and thus can be used for a quick assessment of the natural frequencies of both the real turbines and the small-scale models. Furthermore, a relationship between the diagonal and non-diagonal element in the stiffness matrix is introduced, so that the foundation stiffness can be obtained from either the p-y analysis or the loading test. The results of the proposed expression are compared with the measured frequencies of six real or model turbines reported in the literature. The comparison shows that the proposed analytical expression predicts the natural frequency with reasonable accuracy. For two of the model turbines, some errors were observed which might be attributed to the difference between the dynamic and static modulus of saturated soils. The proposed analytical solution is quite simple to use, and it is shown to be more reasonable than the analytical and the empirical formulas available in the literature.

中国的离婚损害赔偿制度

薛??(Xue ning lan) 한국가족법학회 2008 가족법연구 Vol.22 No.1

스마트폰의 지속적 사용의도에 영향을 미치는 사용자 경험요인에 관한 실증적 연구

녕셜 ( Xue Ning ),김기수 ( Ki Su Kim ) 아시아.유럽미래학회 2012 유라시아연구 Vol.9 No.4

스마트폰은 모바일폰의 새로운 세대로 유비쿼트스 컴퓨팅 기술의 좋은 예이다. 스마트폰을 사용해서 사용자들은 편리한 터치스크린 방식의 인터페이스로 언제 어디서나 인터넷을 사용할 수 있게 되었다. 사용자들은 또한 단순한 전화나 문자 메시지보다 더 효율적인 방식으로 친구들과 연결하고 소통할 수 있게 되었다. 뿐만 아니라 우수한 성능의 하드웨어와 다양한 소프트웨어의 개발로 스마트폰은 업무나 오락 등 다양한 분야를 지원하고 있다 (Charlesworth, 2009). Verkasalo 등(2010)은 스마트폰은 단순한 모바 일폰으로 뿐만 아니라 손안에 들어가는 컴퓨터로 사용될 수 있는 기기라고 지적했다. 이들은 또한 스마 트폰을 통제하는 것은 이제 기기가 아니라 사용자라고 주장하고 있다. 사용자들이 스마트폰과 상호작용할 때 그들의 스마폰 사용 경험은 그 상호작용으로부터 나온다. Goto는 사용자경험(user experience; UX)을 “어떤 기업이나, 서비스 또는 제품에 대해 개인이 가지고 있는 전반적인 인식이나 포괄적인 상호작용”으로 정의하였다 (Wang et. al. 2008). 경험은 사람의 마음에 존재하는 사람과 세상 사이의 눈에 보이지 않는 상호작용 과정이고, 새로운 상호작용들에 의해 유발된다. 이들 상호작용은 육체적, 감각적, 인지적, 감정적, 그리고 미적과 같은 다양한 경험들을 만들어낸다 (Davis, 1992). 이와 같은 관점에서 스마트폰 사용 후 그 기능들에 대한 사용자들의 경험과 사용자들의 사회적 경험은 스마트폰의 지속적 사용에 대한 의사결정에 중요한 역할을 할 것이다. 스마트폰 사용자 수의 증가와 다양한 스마트폰 제조기업과 서비스제공 기업들 사이의 경쟁이 증가하는 상황에서 스마트폰의 지속적 사용의도를 지배하는 요인들과 그들의 역학관계를 이해하는 것은 중요하다고 판단된다. 그러나 스마트폰 이용과 관련한 대부분의 선행연구들은 효율적 인터페이스의 개발, 사용 가능한 시스템의 개발, 그리고 관련 기술의 개발과 같이 사용자의 심리적인 관점에 대해 연구하기보다는 스마트폰 자체의 기능에 더 초점을 맞추었다 (Lin and Ye, 2009; Abowd et. al., 2005; Verstockt et. al., 2009). 반면, 지난 몇 년간 사용자 경험에 대한 포괄적인 이론과 연구의 틀을 제공하기 위한 다양한 시도가 있었다 (Swallow et. al., 2005; Deng and Li, 2008; Wang et. al. 2008; Oza et. al. 2010). 사람들은 스마트폰 이용에 점점 더 많은 시간을 소비하기 때문에 사용자들의 스마트폰 사용겸험이 그들의 스마트폰에 대한 지속적 사용의도를 도출하는데 사용자들이 경험하는 인지과정에 핵심요소가 될 것이다. 이와 같은 이론적 기반 위에 선행연구들과는 다르게 스미트폰의 지속적 사용의도를 설명하기 위해 사용자경험이라는 개념을 선행변수로 사용하였다. 따라서 본 연구에서는 사용자 태도와 행위를 판단하기 위해 사용자경험에 초점을 맞추었다. 사용자경험은 기능적 경험과 사회적 경험 두 가지 관점으로 표현하였다. 기능적 경험에는 이용가능성(usability)과 보안성(security) 그리고 사회적 경험에는 사회성(sociability)과 사회적 주체성(social identity)이 포함되었다. 본 연구에서는 이들 사용자경험 요인들을 선행변수로 스마폰의 지속적 이용의도를 결과변수로 하고, 두 변수를 매개변수로 하는 하나의 인과관계(causal) 연구모형을 제시하였다. 구체적으로 인지된 즐거움(perceived enjoyment: PE)과 인지된 유용성(perceived usefulness: PU)을 선행변수인 사용자경험 요인들과 스마트폰의 지속적 이용의도 사이의 매개변수로 고려하였다. 그 이유는 최근 스마트폰은 업무를 위한 기능적 목적뿐만 아니라 오락이나 사회적 관계(형성)을 위해서도 널리 사용되고 있기 때문에 PU와 PE가 스마트폰의 지속적 이용의도에 중요한 영향을 미칠 것이라는 판단 때문이다. 이 연구모형을 실증적으로 검증하기 위해 다양한 스마트폰 사용자들을 대상으로 설문조사를 실행하였다. 실증연구 결과 기능적 경험과 사회적 경험 모두 인지된 유용성 또는 인지된 즐거움을 통해 지속적 사용의도에 영향을 미치는 것으로 나타났다. 보다 구체적으로 기능적 경험 가운데 이용가능성 요인과 사회적 경험 가운데 사회성 요인은 인지된 유용성과 인지된 즐거움 모두에 유의한 정의 영향을 미치는 것으로 나타났다. 그러나 기능적 경험 가운데 보안성은 인지된 즐거움에만, 사회적 경험 가운데 사회적 주체성은 인지된 유용성에만 유의한 정의 영향을 미치는 것으로 나타났다. 그리고 인지된 즐거움은 인지된 유용성에 유의한 정의 영향을 미치고, 인지된 유용성과 인지된 즐거움 모두 지속적 사용의도에 유의한정의 영향을 미치는 것으로 나타났다. 따라서 스마트폰의 지속적 사용의도를 높이기 위해서는 사용자들의 기능적 경험 가운데 이용가능성과 사회적 경험 가운데 사회성을 높임으로 인지된 유용성과 인지된 즐거움을 높이려는 노력이 중요하다고 판단할 수 있다. 본 연구는 다음과 같은 면에서 학술적이고 실무적인 기여를 했다고 할 수 있다. 첫째, 스마트폰의 지속적인 이용의도를 설명하기 위해 지금까지 경영정보 분야에서 상대적으로 새로운 개념인 사용자경험이란 개념을 사용했다는 점이다. 나아가서 여러 사용자경험 요인들 가운데 스마트폰의 특성을 고려해서 기능적 경험과 사회적 경험 요인들을 고려했다. 또한 본 논문은 스마트폰의 지속적 이용의도에 영향을 미치는 사용자경험 요인이 무엇인지, 그리고 이들 요인들이 어떠한 역학관계로 지속적 이용의도에 영향을 미 치는 지를 실증적으로 분석함으로써 스마트폰 서비스 제공자들에게 유용한 실무적 정보를 제공해 준다. 그러나 본 연구에는 몇 가지 한계가 있다. 우선 사용자경험은 아주 방대한 개념이지만 본 연구에서는 단지 그 일부만 다루었다. 사용자경험의 다른 측면들은 추후 연구들에서 지속적으로 다루어질 수 있을 것으로 생각한다. 또한 사용자경험의 다양한 측면들을 고려하여 사용자경험에 대한 보다 체계적인 연구 가 추후 필요할 것으로 판단된다. 본 연구에 포함된 실증 자료에는 상대적으로 많은 수의 대학생들이 포 함되어 있어 분석결과를 일반화하는 데는 한계가 있을 수 있다. 본 연구가 시행될 때에는 대학생이외 스 마트폰을 사용하는 다른 그룹의 사람들 수가 많지 않아서 상대적으로 많은 수의 대학생이 표본에 포함 되었으나 추후 연구에서는 보다 다양한 사람들을 대상으로 자료를 수집할 필요가 있다. 또한 본 연구에 서는 한국의 스마트폰 사용자를 대상으로 실증분석을 하였기 때문에 문화 및 다른 환경의 차이에 따른 편향성(bias) 문제가 발생할 수 있다. 따라서 추후 연구에서는 다양한 국가의 사용자들을 대상으로 실증 분석하거나 국가별 비교분석도 가능할 것이다. Smartphone is a new generation of mobile phones and it is a good example of ubiquitous computing technology. Users can surf the internet anytime and anywhere through the easy touch-screen interface. Also users can connect with their friends in more efficient ways than calling or texting messages. Besides, its prominent hardware and software support all kinds of applications for work and entertainment (Charlesworth, 2009). Verkasalo et al. (2010) pointed that Smartphone is a device that can be used as a mobile phone as well as a handheld computer, they also argued that it is the user rather than the device who controls the Smartphone. When users interact with Smartphone, their experience of using Smartphone is derived from such interactions. Goto, K. defines User Experience (UX) as "the overall perception and comprehensive interaction an individual has with a company, service or product" (Wang et al., 2008). Experience is an intangible process of interaction between people and the world that exists in humans`` minds and is triggered by new interactions. These may create different types of experiences such as physical, sensual, cognitive, emotional, and aesthetic (Davis, 1992). From this aspect, after using Smartphone, users`` experience on the functions of it and their social experiences with it might be very important in decid- ing if they continue to use Smartphones. Given the growth of the number of Smartphone users and the competition among Smartphone manufacturers and service providers, it is important to understand the dynamics that govern the continued usage intention of Smartphone. However, previous researches on this topic have mostly focused on problems such as creating efficient interfaces, developing usable systems, and establishing technologies, which paid more attention to the facet of functions of the Smartphone itself rather than studying from users`` psychological perspective (Lin and Ye, 2009; Abowd et al., 2005; Verstockt et al., 2009). On the other hand, over the last few years there have been various attempts to provide a comprehensive theory or framework of user experience (Swallow et al., 2005; Wang et al., 2008; Oza et al., 2010; Deng and Li, 2008). Since people spend more and more time with Smartphones, the experience users have with Smartphones is likely to be a key factor in the cognitive process users experience in developing their continued usage intention. Upon this basis, in contrast to previous researches, we employ the concept of User Experience (UX) as the antecedents in this study to explain the continued usage intention of Smartphone, Therefore, this study focuses on users`` experience to assess their further attitudes and behaviors. Users’ experience is represented in two aspects, one is functional experience which includes usability and security, and the other is social experience which includes sociability and social identity. A research model was developed to conceptualize the relationships between these UX factors as antecedent variables and continued usage intention of Smartphons as consequent variables with two intermediary variables. Specifically, we considered perceived enjoyment (PE) and perceived usefulness (PU) as mediating factors between UX factors and the continued usage intention of Smartphone (CUI) since it is widely used not only for functional purposes but for entertainment and social relations. Then our research model was tested empirically by using a survey data collected from diverse Smartphone users. Our results suggest that experience about functional factors and social factors of Smartphone after using Smartphones can influence the perceived usefulness and perceived enjoyment of their usage, which, in turn, influence users`` continued usage intention of Smartphones. More specifically, we found that usability has a significant positive impact on both PU and PE, while security has a strong effect on PE, although no such significant impact was observed on PU. Some previous researchers pointed out that Smartphones are vulnerable and, therefore, security is a very important factor influencing the perceived usefulness of Smartphones. However, security did not affect perceived usefulness of Smartphones significantly in our result. A plausible explanation for this result might be that approximately 60% of our survey respondents are students and they do not consider security issue seriously because they use Smartphones mostly for entertainment or social interactions rather than for job or work related purposes. However, it does not mean security issue is not important for usefulness of Smartphones or the current level of security of Smartphone is perfect. Moreover, sociability is found to affect both PE and PU, while social identity have significant impact on PU only. Besides, PE showed a significant effect on perceived usefulness of using Smartphone and both PE and PU positively affected CUI. Main contributions of this research are as following. First the concept of user experience, which is a relatively new concept in MIS researches, was adopted to explain users’ continued intention to use Smartphones. Moreover, not only functional experience but social experience was included as user experience factors. This paper also makes contributions in understanding the important factors affecting users’ continued usage intention of Smartphones and the dynamics of these effects, which can provide useful information for Smartphone service providers. There are, however, some limitations inherent in this study. Although user experience is a broad concept, we focused only a part of it. Other aspects of user experience may be considered in future researches. More systematic research on user experience may be also needed related with Smartphone usage. In our survey data, relatively large number of university students were included, therefore, we have limitation in generalizing the results. Since the survey data were gathered from Korean, the analysis results may be subject to culture and/or other environmental biases. In future researches, we may use survey data from users of different countries and also implement a cross country analysis.

Proline accumulation and transcriptional regulation of praline biothesynthesis and degradation in Brassica napus

( Xing Ning Xue ),( Ai Hua Liu ),( Xue Jun Hua ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.1

Mechanochemistry synthesis of high purity lithium carbonate

Ning Chen,E Zhou,Dong-ping Duan,Xue-min Yang 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.10

A technique for preparing high purity Li2CO3 powders has been developed through mechanochemical process coupled with the dissolution-filtration process. The first step as mechanochemical reaction of both Na2CO3 and LiCl mixtures was designed to obtain the primary Li2CO3 powders using a ball mill. The second step as the dissolution- filtration process was performed to obtain high purity Li2CO3 powders. Experimental results indicate that the three parameters of milling time, rotation speed, and ball-to-sample mass ratio can closely relate with purity of primary Li2CO3 powders. The XRD patterns of primary Li2CO3 powders indicate that mechanochemical reaction of both Na2CO3 and LiCl can be completed in 15 min under optimal conditions at rotation speed as 600 rpm, ball-to-sample mass ratio as 5/1, and molar ratio of Na2CO3 to LiCl as 1/2. The target products of Li2CO3 powders contain impurity of Na+ less than 0.1mass% with the minimum values as 0.073mass%. Two shapes of massive particles and smaller grains less than 1 μm in nano scale can be observed in the target products of Li2CO3 powders.