A Basic Study on Leaf and Stem Production of Angelica acutiloba

Choi,Seong-Kyu,Yun,Kyeong-won,Chon,Sang-Uk,Lee,Jong-Ill,Seo,Young-Nam,Seo,Kyoung-Sun,Choi,Kyeong-Ju 한국자원식물학회 2004 Plant Resources Vol.7 No.3

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10%, while germination rate of seeds collected in 2004 was above 90%. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4×4×5cm(length×width×height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.

전신성 백색증(Oculocutaneous albinism) 환아의 증례 보고

최지은,최남기,김선미,양규호 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

백색증(albinism)이란 신체 내 melanin 합성의 부분적,혹은 완전한 결핍에 의해 일어나는 질환이다. Melanin은 신체 내에서 머리카락,눈,피부 등에 분포하기 때문에 백색증 환자는 밝은 금발이나 갈색의 머리카락과 하얀 피부,붉거나 갈색의 홍채를 갖는 특징적인 외모를 지닌다. 백색증 환자는 변이된 유전자 및 임상적 특징에 따라 크게 전신성 백색증,안성 백색증,부분적 백색증으로 나뉘며 그 밖에 많은 하위군이 존재하고 그에 따른 치료법이나 치과적 주의사항도 달라진다. 본 증례는치아의 발육지연 여부평가를 주소로 내원한정신 지체가 동반된 전신성 백색증 환아로서 치아발거 등의 관혈적 시술및 보존수복,공간유지장치 등의 치료를 통해 양호한 결과를 보였기에 이를 보고하는 바이다. Albinism is a disease caused by partial or complete failure of melanin production in the skin,hair and eyes despite the presence of normal number,structure and distribution of melanocytes. Typical aspects are white-colored skin,blonde-brown hair,blue-brown irides and a prominent red reflex. Three main categories of albinism are oculocutaneous,ocular and localized albinism,and also they are divided into many subgroups. Therefore, appropriate treatment plan and dental direction would be differentiated according to them. This case report was about oral conditions and treatment of the oculocutaneous albinism patient with mental retardation who was refered due to developmental delay of teeth and treated with teeth extraction,restoration and space maintenance etc.

4세 유아에 발생한 몰입성 하마종(plunging ranula); 증례보고

최진호,양동환,김일규,오남식 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.4

Whereas oral ranula is relatively common and presents as a cyst in the mouth, the plunging ranula is rare and manifests itself as a mass in the neck with or without an associated oral lesion. When there is a soft anterior neck swelling without oral swelling, diagnosis is still difficult. In such a case, clinical suspicion may be low and the pathologist may be misled by the histologic appearance. Recognition of the diagnosis of plunging ranula is essential for the correct treatment of these lesions We report a case of a 4-year-old girl and review the literatures to discuss the differential diagnosis and treatment modalities.

흰쥐에서 심정지후 뇌의 허혈-재관류 손상과 Tumor Necrosis Factor-α의 상관관계

최승필,박규남,박승현,박상현,정시경,김세경 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : Tumor necrosis factor-α(TNF-α) has been thought to play a major role in neurological injury during global brain ischemia and subsequent reperfusion following resuscitation in cardiac arrest. So, we hypothesized that the elevation in TNF-α was dependent upon the duration of the global brain ischemia, and related to delayed neuronal damage. Methods : Fourteen rats were divided two groups ; 1 minute-cardiac arrest group(n=7) and 3 minute-cardiac arrest group(n=7). we induced cardiac arrest by chest compression and clamping of tracheal tube for 1 minute and 3 minutes respectively. And then, resuscitation was initiated. To measure the plasma activity of TNF-α, blood samples were drawn before and at the end of cardiac arrest, and 30, 60, 90, and 120 minutes after initiation reperfusion. At 72 hours after resuscitation, the ND(neurologic deficit) score was determined and the histopathologic outcome of hippocampal CA1 neuron was observed by the percent dead hippocampal CA1 neurons. Results : 1. TNF-α level during the early reperfusion period(<2h) was significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(p=0.0001). 2. There was a no significant difference of neurologic deficit score between 1 min-and 3 min-cardiac arrest. 3. Percent dead hippocampal neurons were significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(9.1±1.2% vs 1.2±0.9%, p<0.05). Conclusions : The results suggest that longer duration of global brain ischemia causes a more profound increase in plasma TNF-α level during the early reperfusion period(<2h) and more delayed neuronal damage than lessor duration of global brain ischemia, and that increase in TNF-α level during the early reperfusion period(<2h) is related to delayed neuronal damage.

백서의 원위부 피편에 동맥유입의 중요성에 관한 실험적 연구

최규남,최희윤,강희문,김한중 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.2

Microsurgery and myocutaneous flap have been developed recently for wide employment in plastic and reconstructive surgery. Therefore, the delay procedure has been declined in its use recently, because it has many difficult problems. If the flap is longer and larger, it should be delayed for complete survival, but delay requires considerable time. Therefore, as a substitute for delay, vascular anastomosis in the distal part of a flap will theoretically give the same effect. In this study, we investigated the importance of arterial inflow in the distal side of a flap in rats. The experiment was done in 3 different groups according to the design of flaps and in each group 3 subgroups were made. In A flap, the superficial epigastric artery was preserved or anastomosis. In V flap. the superficial epigastric vein was preserved or anastomosis. In C flap, as a control flap, both artery and vein were severed. In result, A flaps in all 3 groups were completely survived. Both V and C flaps in all 3 groups were showed distal necrosis. But C flaps were showed more necrosis than V flaps. In conclusion, we suggest the possibility that a large flap may be transposed in one stage with vascular anastomosis in the distal part-preferably artery and vein, or artery only.

Effect of Activated Charcoal on Growth of Zingiber mioga ROSC

Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-Sun,Kwon,Byung-Sun 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Activate charcoal (AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Zingiber mioga ROSC as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated charcoal added with 10-30%, suggesting that optimum amount of activated charcoal was ranged from 10 to 30%. Growth and enlargement of the root were improved by 10% AC with higher rhizome length and weight.

이유백서에서 교근절제후 하악과두의 면역조직화학적 연구

최남기,양규호,최홍란 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2

The purpose of this study was to determine whether there was any correlation between temporomandibular joint dysfunction and structure of the mandibular condyle. Weanling rats had their masseter muscles resected and immunohstochemical findings were observed with a light microscope. The results obtained were as follows: 1.The condylar cartilage region was divided into articular,proliferating,cartilage cell and hypertrophic cell layers according to cell morphology. 2.In light microscopic views,the proliferating and cartilage cell layers of the experimental group decreased gradually and at the 8th week significantly. 3.In immunohistochemical staining for type Ⅰand Ⅱ collagen,a reaction was detected in the lower part of proliferating cell and cartilage cell layers.In the cartilage cell layers,a stronger cellular reaction was present.Immunohistochemical staining for type Ⅱ collagen reacted more strongly than that Ⅰ collagen. 4.In immunohistochemical staining for proteoglycan,the staining of the experimental group resembled the control group and gradually showed a weak reaction.The proliferating and cartilage cell layers reacted more strongly then hypertrophic cell layer. 5.In immunohistochemical staining for proliferating cell nuclear antigen(PCNA),the strong reaction was detected in the nucleus of the proliferating cell layer both in control and experimental groups.But the thickness of the proliferating layer decreased in experimental group,consequently the reaction of the experimental group was reduced more than that of the control group.

Effect of Storage Condition on Yield and Quality of Angelica acutiloba Radix

Choi,Seong-kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-nam 한국자원식물학회 2002 Plant Resources Vol.5 No.1

To develop an optimal storage method of root of Angelica acutiloba Radix, which has been grown as major herbal medicine material to be cultivated, root of Angelica acutiloba Radix, was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. In conclusion, vacuum packing and complete sealing with polyethylene film appears to be most optimal for storage of Angelica acutiloba Radix.

이유백서에서 교근절제후 하악과두의 면역조직화학적 연구

최남기,양규호 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

The purpose of this study was to determine whether there was any correlation between temporomandibular joint dysfunction and structure of the mandibular condyle. Weanling rats had their masseter muscles resected and immunohistochemical findings were observed a light microscope. The results obtained were as follows : 1. The condylar cartilage region was divided into articular, proliferating, cartilage cell and hypertrophic cell layers according to cell morphology. 2. In light microscopic views, the proliferating and cartilage cell layers of the experimental group decreased gradually and at the 8the week significantly. 3. In immunohistochemical staining for type Ⅰ and Ⅱ collagen, a reaction was detected in the lower part of proliferating cell and cartilage cell layers. In the acrtilage cell layers, a stronger cellular reaction was present. Immunohistochemical staining for type Ⅱ collagen reacted more strongly than that of type Ⅰ collagen. 4. In immunohistochemical staining for proteoglycan, the staining of the experimental group resembled the control group and gradually showed a weak reaction. The proliferating and cartilage cell layers reacted more strongly than the hypertrophic cell layer. 5. In immunohistochemical staining for proliferating cell nuclear antigen(PCNA), the strong reaction was detected in the nucleus of the proliferating cell layer both in control and experimental groups. But the thickness of the proliferating layer decreased in experimental group, consequently the reaction of the experimental group was reduced more than that of the control group.

Study on Leaf Production of Angelica acutiloba by Mulching with Polyethylene Film

Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-sun,Kwon,Byung-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3

This study was conducted to produce effectively more leaves of Angelica acutiloba through year-round production system by using various mulching materials and polyethylene(PE) tunnel. Soil temperature by covering treatment was higher in PE mulching and PE tunnel than in rice straw mulching, especially showing the highest temperature in PE tunnel from April through May. Water content of soil was higher in PE mulching than in non-mulching. Leaf production of Angelica acutiloba through year-round production showed the highest in PE tunnel and root production in PE mulching. These results suggest that leaf production of Angelica acutiloba can be maximized through PE tunnel cultivation system.